Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	23,270	G→A	G294S (GGC→AGC)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	23,270	0	G	A	98.9%	259.4 / NA	90	G294S (GGC→AGC)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (49/40);  minor base C (1/0);  total (50/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

GTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCAC‑CACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTG‑AGCTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTG  >  NC_000913/23024‑23512                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      gTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCAc                                                                                                                                                                                                                                                   >  1:284427/1‑251 (MQ=255)        ttcaggcgagcGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGTCCCAATCTCGCTGGAGATCGGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGGCAGGCCGTGATTCTGGCGAAAGATCTTGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTaaa                                                                                                                                                                                                                                             <  2:135232/240‑1 (MQ=255)              cggTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGCCGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGGTTACACCATTTTCAGGACGGGAAAAGGTggg                                                                                                                                                                                                                                      >  1:363990/3‑249 (MQ=255)              cggTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGCTTACACCATTCTCCGCACGGTACAAGGGGCg                                                                                                                                                                                                                                      >  2:479274/3‑251 (MQ=255)                    aTCAGGATGTACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTTATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCtt                                                                                                                                                                                                                                <  2:220236/251‑1 (MQ=255)                    aTCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCtt                                                                                                                                                                                                                                <  1:337737/251‑1 (MQ=255)                     tCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGGAAAAGGTGCGGAGCTTg                                                                                                                                                                                                                               >  1:524652/1‑251 (MQ=255)                      ccggATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGAATCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTAAACCATTCACAGCACGGTAAAAAGGGCGGGGCTTg                                                                                                                                                                                                                               >  2:360699/3‑250 (MQ=255)                       aGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTTGTGATCTGGACCACTCA‑GCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACTTGATTTCGACTATGCGCTGGTGCAGAGCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCGCAGCACGGTAAAAGGTGCGGAGCTTG‑Ag                                                                                                                                                                                                                            <  2:331542/251‑1 (MQ=255)                                  aaaGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCtt                                                                                                                                                                                                                 <  1:231411/251‑1 (MQ=255)                                  aaaGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCtt                                                                                                                                                                                                                 >  1:459263/1‑251 (MQ=255)                                     gCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑c                                                                                                                                                                                                             >  1:638480/1‑251 (MQ=255)                                        aaaTTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCTCCGGGCAGGCCGTGATTCTGGCGAAATATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTTACCGATTAAACCCTTCTCAACACGGTAAAATGAGCGGAGCTTG‑ATTTGCTGCGCTTTA‑ccc                                                                                                                                                                                                           >  2:669952/1‑250 (MQ=255)                                         aaTTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACGCCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCAt                                                                                                                                                                                                         <  1:292442/251‑1 (MQ=255)                                              cccGTAAGCAACGTTAACGGCCCAATCTCGCTGGGGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCGGATTTCGCCTATGCGCTGGTGCAGAGCGACGGTCAGGCCGTGATTCTGGCGAAAGAGCTGGTTGAAAGCGTAATTCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCAGCCGtt                                                                                                                                                                                                    <  2:626852/250‑1 (MQ=255)                                                  aaaGCAACGTGAACGGCCCAATCTCGCTGGGGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCAGCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATg                                                                                                                                                                                                <  2:176596/250‑1 (MQ=255)                                                     gctaCGTGAACGGCCCAATCTCTCTGTTGATCTGGACCAC‑TACTCCGTGGACTCTGCCGTCGAACCGCGCAATCTCTATGGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGTCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATTCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCTGAGCTTg                                                                                                                                                                                                                               <  2:588961/216‑1 (MQ=255)                                                          gTTAACGGCCCAATCCCGCTGGGGATCTGGACCAC‑TACGCCGTTGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGa                                                                                                                                                                                        <  2:524652/251‑1 (MQ=255)                                                            tAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGGAAAAGGGGCGGAGCTTG‑AGTGGCTGCGCTTTA‑CCCATCCGGTTAAGGGCTTCGACg                                                                                                                                                                                      >  2:137896/1‑251 (MQ=255)                                                               cGGCCCAATCTCGCTGGGTATCTGGACCCC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGGGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCAGTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTc                                                                                                                                                                                   <  2:438627/251‑1 (MQ=255)                                                                       tctcGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGAACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGGTTATGGGCTTCGACGTTCCGGCAATc                                                                                                                                                                           >  1:275842/1‑251 (MQ=255)                                                                         tcGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCt                                                                                                                                                                         >  1:288800/1‑251 (MQ=255)                                                                                  aTCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATc                                                                                                                                                                >  1:334561/1‑250 (MQ=255)                                                                                  aTCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTAACCGATTACAACATTCTCAGCACGGGAAAAGGGGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCCTCCGTTTTTGGGCTTCGACGTTACGGCAATACTCgggcattc                                                                                                                                                                >  2:83258/1‑245 (MQ=255)                                                                                         ccac‑taCGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTAcc                                                                                                                                                        >  1:339763/1‑251 (MQ=255)                                                                                           cactacGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCATTCCCCGGCA‑ATCACGTTAccc                                                                                                                                                       >  1:365022/5‑251 (MQ=255)                                                                                            actacGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCt                                                                                                                                                      >  1:343709/4‑251 (MQ=255)                                                                                            actacGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGGCCGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGCTTACAAAATTCTCAGCACGGGACAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCACTCCGTTTATGGGCCTCGACGTTCCGACAATACTCGGCG‑ATCACGTAACCCt                                                                                                                                                      >  2:624726/4‑251 (MQ=255)                                                                                                    gTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTTCACCATTCTCAGCACGGGTAAAGGTGCGGCGCTTG‑GGTTGCTGCGCTTTA‑CCCATCCGTTTCTGGGCTTAGTCGTTCCGGCAATCCTCGGCG‑ATCACGTTTCCCTggtttccg                                                                                                                                              >  2:286448/1‑245 (MQ=255)                                                                                                           ctgcctgcGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTAcc                                                                                                                                         <  1:83258/249‑1 (MQ=255)                                                                                                           ctgcctgcGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTAcc                                                                                                                                         <  2:104273/249‑1 (MQ=255)                                                                                                              cctgcgAACCGCGCTAACTTTATTGAGCCTGATTTCGACTACGCGCTGGTGCAGATCGACGGTCAGGGCGTGATTCTGGCGAAAGATCTGGTTTACAGCGTAATGCAGCGTATCGGCGTGACCGATTACGCCATTCTCAGCACGGTAAAAGGTGCGGATCTTG‑AGTGGCTGCGCTTTC‑CCTGACCGTTTATTGGCGTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCttgacgtcggtctcggtgt                                                                                                                                    <  2:449555/251‑19 (MQ=255)                                                                                                               ctgcgAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGttt                                                                                                                                                                                                   <  1:455757/188‑1 (MQ=255)                                                                                                               ctgcgAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGAAAAAGGTGCGGGGCTTG‑GGTTGCTGCGCTTTA‑CCCATCCGttt                                                                                                                                                                                                   >  2:455757/1‑188 (MQ=255)                                                                                                                   gaaCCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCAGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTc                                                                                                                               >  1:623105/2‑251 (MQ=255)                                                                                                                       cgcgAAATCTCTATTGCACCTGCTTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACAGATTACAACATTCTAAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CACATCCGTTTATGGGCTTCGACGATCCGGCAATACTCGGCG‑ATCACGTTACCCTGCATGACGGAAACGGTGCCGATCacac                                                                                                                           >  1:111556/1‑251 (MQ=255)                                                                                                                         cgcAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCCCAccc                                                                                                                         >  1:315340/1‑250 (MQ=255)                                                                                                                          gcAATCTCTATTGCACATGATTTCGACTATGCGCTGGCGCAGATCGACGGTCAGGCCGTGATTTTAGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATAACCACATTCACAGAACGGTAAAAGGCGCTGAGCTTGGATTTGC‑GCGCTTTAACCCCTC‑GCTTATTGGCTTCGACGTTACGGCAATCCACCGCGTACCA‑GTTACCCTGGATGCCGGTAccctgggcgttccacacac                                                                                                                        >  2:308210/1‑234 (MQ=255)                                                                                                                                 ctATTGCACCTGATTTCGACGATGCGCTGGTGCAGATCGACGTGCAGGCCGTGATTCTGGCGAAAGAGCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCt                                                                                                                    <  2:340027/248‑1 (MQ=255)                                                                                                                                   atgtcACCTGATTCCGATTATGCGCCGGTGCAGATCGACGGTCAGACCGGGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGTGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCTCTTTA‑CCCATCCGATTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGGGCCGTTCACACCGCGCCTGGCCa                                                                                                               <  2:31043/247‑1 (MQ=255)                                                                                                                                              tttCGACTATGCGCTGGTGCAGATCGACGGTAATGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGCCCGATTACACCATTCTCAGCACGGTAAAAGGTACGGAGCTTG‑AGATGAGTCGCTTTA‑CCCCTCCGTTTAAGGTACTCGACGTTCCGGATATAACCGGCG‑ATAACGTAACCCTGTATGCCGGAGCAGGTGCCGTTCACACAGCGcccgcgcagcgcccggccg                                                                                                    >  2:129474/1‑234 (MQ=255)                                                                                                                                                 cGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCAGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGgacgac                                                                                                  <  2:623105/250‑1 (MQ=255)                                                                                                                                                       tgcgttgggtctgATCGACGGTCTGGCCGTGATTCTGGCGAAAGATCTGGTTGCAAGCGTTATGCTGATGATCGTCTTGACCGATAACACCATGCTCAGCACGGTAAAAGTTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCACCCGTGTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTCCCCTGGATGCCGGTTCCGGGGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTAtgtg                                                                                            <  2:510985/238‑1 (MQ=255)                                                                                                                                                        gcgcTGGGGCAGATCGACGGTCAGGCCGGAATTCTGGCGAAAGATCTGGTTGAAAGCGTAAGGCAGCGTATCGGCGTGACCGATTAAACCATTCTCATCACGGTAAAAGGGGCGGAGCTTG‑GGTTGCTGCGCTTTA‑ACCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGACG‑AACACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGGAGACTAtgtgt                                                                                           >  1:228022/1‑249 (MQ=255)                                                                                                                                                         cgcTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATc                                                                                         >  1:445118/1‑251 (MQ=255)                                                                                                                                                          gcTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGAGTGACCGAATACACCATTCTCAGCACGGGAAAAGGAGCGGAGCTTG‑AGATGCTGCGCTTTA‑ACCATACGTTTATGGGCTTCGACGTTACGGAAATCCTCGGGG‑AGCCCGTTACCCTGGATGCAGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGGCCGGCCGACTATGTGATCg                                                                                        >  1:445472/1‑251 (MQ=255)                                                                                                                                                           ggggTGCATATCGTCGTTCAGGCCGTGATTCTGGCGAAAGATGTGGTTGAAAGTGTAATGCAGCGTAGCGGCGTGACCGATTACACCATTCCCAGCACGGTAATAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGGCGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCTGGACGACTATGTTATCgg                                                                                       <  2:401310/249‑1 (MQ=255)                                                                                                                                                           cTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGCCGACTATGGGATCgg                                                                                       >  1:537973/1‑251 (MQ=255)                                                                                                                                                           cTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGGTGCTGCGCTTTA‑CCCATCCGGTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCgg                                                                                       >  1:573958/1‑251 (MQ=255)                                                                                                                                                             ggTGCAGAGCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTGCCCTGGATGCCTGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGt                                                                                      <  1:308210/250‑1 (MQ=255)                                                                                                                                                                 cAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCCCGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCCTCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGCCGACTATGTTATCGGTCAGa                                                                                  >  1:274972/1‑250 (MQ=255)                                                                                                                                                                  aGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGaaa                                                                                <  1:196756/251‑1 (MQ=255)                                                                                                                                                                         cGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGcc                                                                         <  1:541079/251‑1 (MQ=255)                                                                                                                                                                             catgacGTGTTTCTGACGAAAGATCTGGTGGAAAGCGTAATGCAGCGTATCGGCTGGACCTATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGTCGTTCCGGCAATCCGCGGCG‑ATCACGTTTCCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCttg                                                                      <  1:122374/245‑3 (MQ=255)                                                                                                                                                                             cAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑TGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTgg                                                                      <  1:122376/250‑1 (MQ=255)                                                                                                                                                                             cAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGCTCGGTCAGAAATACGGCCTggg                                                                     >  1:303345/1‑250 (MQ=255)                                                                                                                                                                               ggCCTTGATTCTGGCGAAAGATCTGGGTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTTCACCATTCTCAGCACGGTAAAAGGTGCTGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑AGCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGaaa                                                                   <  2:230020/251‑1 (MQ=255)                                                                                                                                                                                 ccGTGAGTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAAcc                                                                 <  2:59892/251‑1 (MQ=255)                                                                                                                                                                                  cGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGCCGACTATGTGATCGGTCAGAAATACGGCCTGGAAAccc                                                                >  1:259523/1‑250 (MQ=255)                                                                                                                                                                                  cGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCg                                                                >  1:264894/1‑251 (MQ=255)                                                                                                                                                                                   gTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGGTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCCCCTGGCCACGGCCCGGACGCCTATTGGAGCGGTCAGAAATACAGCCTGGCAAcagc                                                               >  2:131022/1‑248 (MQ=255)                                                                                                                                                                                    tGATTCTGGCGAAAGATCTGGTTGAAAGCGAAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGCGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTAGGCG‑ATCACGTTTACCTGGATGCCGGAACCGGTGCCGTTTACACCGCGCCTTGCCACCGCCCGGACGACCATGTGATCGGTCAGAAATACCGACAGGAAACCGcc                                                              >  2:521979/1‑250 (MQ=255)                                                                                                                                                                                           ggCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCgg                                                       <  1:283476/251‑1 (MQ=255)                                                                                                                                                                                              gcaaGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACCCCATTCTCAGCACGGGAAAAGGTGCGGGGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATTGGCGTCGCCGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCCGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGTTAGGGCAGAAAGACGGCCTGGACACCGCTACACCGGttt                                                    >  2:190066/3‑250 (MQ=255)                                                                                                                                                                                              gAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGGAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTAGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAACTACGGCCTGGGAACCGCTAACCCGGTTg                                                    >  1:73295/1‑251 (MQ=255)                                                                                                                                                                                              gAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTCAACGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTTCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGAACGGGCAGGAATACGGCCTGGAAAcccataaacccgttg                                                    >  2:121969/1‑238 (MQ=255)                                                                                                                                                                                                 aGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAACGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGcc                                                 <  2:166281/251‑1 (MQ=255)                                                                                                                                                                                                     cTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGGCGACTATGTAATCGGGCAGAGATAAGGCCTGGGAAACGCTAACCCGGTTGGCCCggg                                             >  2:654273/1‑250 (MQ=255)                                                                                                                                                                                                       gggtGAAAGCGTAATGCAGCGTAGCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCTGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTGCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGGCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCgg                                              <  2:303345/245‑1 (MQ=255)                                                                                                                                                                                                       ggTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTCAAAGGTGCGGAGCTTG‑ATTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACCCCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCGCGGACg                                           <  1:268204/251‑1 (MQ=255)                                                                                                                                                                                                           gAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGGAAAAGGGGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTAAGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTAACACCGCGACTGGCCACGGCCCGGACGACTATGGGATCGGTCAGAAATACCGCCCGGAAACCGCTAACCCGGTTGGCCCGGACGGCAc                                       >  1:446427/1‑251 (MQ=255)                                                                                                                                                                                                             aaGCGTAATGCAGCGTATCGGCGTGTCCGATTTCACCATTCTCAGCACGGTAAAAGGTGCGGCGCTTG‑CTGTGCTGCGCTTTT‑ACCATCCGTTTATGTGCTTCGACGTTCCGTCAATCCACGGCG‑ATCCCGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTATCCCGGTTGGCCCGGTCGGCCCtt                                     <  2:488134/251‑1 (MQ=255)                                                                                                                                                                                                              aGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTa                                    <  1:669952/251‑1 (MQ=255)                                                                                                                                                                                                               gCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTAt                                   <  1:360699/251‑1 (MQ=255)                                                                                                                                                                                                                cGTAATGCAGCGTATCGGCGTGACCGATTACCCCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGGGCCGTTCACACCGCGCCTGGCCCCCGCCCGGACGGCTATGTGATCGGGCAGAAATAAGGCCTGGAACCCGCTAACCCGGTTGGCCCGGCAGGCACTTATc                                  >  1:413560/1‑251 (MQ=255)                                                                                                                                                                                                                 gTAATGCTGCGTATCGGCGGGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCGGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATa                                                                              <  2:269302/206‑1 (MQ=255)                                                                                                                                                                                                                 gTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGGCAGAAATa                                                                              >  1:269302/1‑206 (MQ=255)                                                                                                                                                                                                                        aGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGGAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGGGATCGGTCAGAAATACGGCCTGGAAAACGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGc                          >  1:658058/1‑251 (MQ=255)                                                                                                                                                                                                                           gTATCGGCGTGACCGATTACCCCATTCTCAGCACGGGAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACt                       <  2:138441/251‑1 (MQ=255)                                                                                                                                                                                                                           gTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACt                       <  1:233262/251‑1 (MQ=255)                                                                                                                                                                                                                               cGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTGG‑AGTTGCTGCGCTTTA‑CCCATCCGTGTATGGGCTTCGACGTACCGGCAATCCTCGGCG‑ATCCCGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTAt                    <  2:288808/250‑1 (MQ=255)                                                                                                                                                                                                                                ggCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGAGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTCACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATcc                  >  1:620648/1‑251 (MQ=255)                                                                                                                                                                                                                                  cGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCg                 >  1:675931/1‑250 (MQ=255)                                                                                                                                                                                                                                   gTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGAc               <  2:334561/251‑1 (MQ=255)                                                                                                                                                                                                                                   gTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATAAGGCCAGGAGACCGCTACCCCCGTTGGCCCGGACGGCACTTATCTGCCGGGCAATTATCCGAc               >  2:537484/1‑251 (MQ=255)                                                                                                                                                                                                                                     gACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑CGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCCAACCCGGTTGGCCCGGACGGAACTTATCTTCCGGGCACTTATCCgccc              >  2:569404/1‑247 (MQ=255)                                                                                                                                                                                                                                            tACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATg       <  2:343709/250‑1 (MQ=255)                                                                                                                                                                                                                                              cacCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCg    >  1:424719/1‑251 (MQ=255)                                                                                                                                                                                                                                              cacCATACTCAGCCCGGAAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGTCCTGGAAACAGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGCCGCTGGCTGGCg    >  2:293062/1‑251 (MQ=255)                                                                                                                                                                                                                                                ccATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCCCTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTg  >  2:341036/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      GTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCAC‑CACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTG‑AGCTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTG  >  NC_000913/23024‑23512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑