Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	23,161	A→T	P257P (CCA→CCT)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	23,161	0	A	T	97.6%	255.9 / ‑1.3	84	P257P (CCA→CCT)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base T (49/32);  total (50/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.10e-01

CACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCAC‑CACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTG‑AGCTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCA  >  NC_000913/22919‑23403                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      cACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCg                                                                                                                                                                                                                                                >  1:487567/1‑250 (MQ=255)  aCCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGAc                                                                                                                                                                                                                                              <  1:53479/251‑1 (MQ=255)   ccTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCACTAGCGGACGCGGAAGTTGAGTCTTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCCACCCGCGCCATCTCTATTGCACCTGCTTTCGACt                                                                                                                                                                                                                                             >  1:421319/1‑251 (MQ=255)      gCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAACATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATg                                                                                                                                                                                                                                          >  1:188603/1‑251 (MQ=255)      gCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATg                                                                                                                                                                                                                                          >  1:138441/1‑251 (MQ=255)         caAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGATGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATgcgg                                                                                                                                                                                                                                       >  2:233262/1‑250 (MQ=255)           aaGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTg                                                                                                                                                                                                                                     >  1:612801/1‑251 (MQ=255)                  gAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGCCGTTGAGTATTACGACCAAACTTCTCCGTCCATCGACGTTGCTTTTCCGGCGGTCGATCAGGATGCACTGCAAGCAAAATTTGCCGTAAGCAACGTTACCGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGAAATCTCTATTGCACCTGATTTCGACTAAGCGCTGGTGccgg                                                                                                                                                                                                                              >  2:283476/1‑248 (MQ=255)                  gAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCACCGTTAACGGCCCCATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGc                                                                                                                                                                                                                                 >  1:400745/1‑248 (MQ=255)                   aaGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGAt                                                                                                                                                                                                                             <  1:600369/251‑1 (MQ=255)                       cggtacACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGCGCTGATCGAc                                                                                                                                                                                                                         >  2:616229/6‑251 (MQ=255)                            cACTGGGGCGTTGCCGGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTCCAGGCAGTCGAACAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCAC‑CACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTc                                                                                                                                                                                                                     <  2:295310/250‑1 (MQ=255)                                 gggcgtggccTGCCGTGCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTGCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCg                                                                                                                                                                                                               <  2:3594/242‑1 (MQ=255)                                    cGTTGACTGCCGCTCTGCGCCGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGGGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGa                                                                                                                                                                                                            <  1:193557/251‑1 (MQ=255)                                        gtcTGCCGATCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACGTCTCCGACCAGCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTGCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTc                                                                                                                                                                                                         <  1:5346/248‑1 (MQ=255)                                          cTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTgg                                                                                                                                                                                                      >  1:449555/1‑251 (MQ=255)                                               gTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGATAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACCCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCCCCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTTTggtcaa                                                                                                                                                                                                  >  2:333784/1‑246 (MQ=255)                                                ttCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGCACCGCGCAATCTCTATTGCCCCTGCTTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGaa                                                                                                                                                                                                  >  1:408925/1‑249 (MQ=255)                                                       cTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGt                                                                                                                                                                                         >  1:331542/1‑251 (MQ=255)                                                            ggaagcggaagTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAAGTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGaaa                                                                                                                                                                                    <  1:475199/251‑1 (MQ=255)                                                              aagcggaagTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGc                                                                                                                                                                                  >  1:104273/1‑251 (MQ=255)                                                                  ggaagTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGCACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGGGCCGATCGACGGTCAGGCCGGGATTCTGGCGAAAGATCTg                                                                                                                                                                                           >  2:283003/1‑238 (MQ=255)                                                                  ggaagTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTg                                                                                                                                                                                           <  1:283003/238‑1 (MQ=255)                                                                      gTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGc                                                                                                                                                                           >  1:438627/1‑250 (MQ=255)                                                                        tGAGTATTACGAAAAAACTTCCCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTGCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAg                                                                                                                                                                         <  2:676222/250‑1 (MQ=255)                                                                           gTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGt                                                                                                                                                                      >  1:79123/1‑250 (MQ=255)                                                                              ttACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCg                                                                                                                                                                  >  1:166640/1‑251 (MQ=255)                                                                                  gACAAAACTTCTCCGTCCATCGACGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCAC‑CACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGt                                                                                                                                                              >  1:253931/1‑251 (MQ=255)                                                                                   aCAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGAGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCg                                                                                                                                                               >  1:430606/1‑249 (MQ=255)                                                                                      aaaCTTCGCCGTCCACCGACTTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGTTGCAGAGCGACGGTCAGGCCGTGAGTCTGGCGAAAGAGCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAcc                                                                                                                                                          <  2:612801/251‑1 (MQ=255)                                                                                             tcCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTac                                                                                                                                                    >  1:288808/1‑250 (MQ=255)                                                                                              ccGTCCATCGCCGTTGCTTTTCCGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAAGCTCTCTGGTGATCTGGACCAC‑TACGCCGTGGACTCGGCCTGCGAACCGCGCAATCTCTATTGCACCTTATTTCGACTATGCGCTGGTGCAGATCGACGGGCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTacac                                                                                                                                                  <  2:381234/251‑1 (MQ=255)                                                                                              ccGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTa                                                                                                                                                     <  1:242566/248‑1 (MQ=255)                                                                                               cGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACAcc                                                                                                                                                 >  1:482421/1‑251 (MQ=255)                                                                                               cGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACAcc                                                                                                                                                 >  1:166281/1‑251 (MQ=255)                                                                                                gTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGCTTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGCTCTGGTTTAAAGCGGAATGCAGCGTATCGGCgaggaccgttaaaccc                                                                                                                                                >  2:196756/1‑236 (MQ=255)                                                                                                gTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGTAGCGTATCGGCGTGAACGATTaaccc                                                                                                                                                 >  2:541079/1‑246 (MQ=255)                                                                                                  ccATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCAtt                                                                                                                                              <  1:236817/251‑1 (MQ=255)                                                                                                   cATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGTCCGTGATTCTGGCGAAAGATCGGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATtc                                                                                                                                             <  1:50283/251‑1 (MQ=255)                                                                                                     ccGACGTTGCTTTTCAGGCCGTAGATCCGGAATCACTGAAATCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTTATCTGGACCAC‑TACGCCGTTGACTCTGCCTGCGAACCGTGCAATCTCTATTGCACCTGATTTCGTCTATGCGCTGGTGCAGATCGACGTTCAGGTTGTGATTCTGGCGAAATATCTGGTTGAAAGCGTAATGCATCGTATCGGCTTGTCCGATTACTCCATtctc                                                                                                                                           <  2:468469/250‑1 (MQ=255)                                                                                                        aCGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATtctcagc                                                                                                                                        <  1:495622/251‑4 (MQ=255)                                                                                                         cGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATtctcagc                                                                                                                                        >  1:59892/1‑247 (MQ=255)                                                                                                          gTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCAc                                                                                                                                      >  1:284427/1‑251 (MQ=255)                                                                                                                 ttcaggcgagcGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGTCCCAATCTCGCTGGAGATCGGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGGCAGGCCGTGATTCTGGCGAAAGATCTTGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTaaa                                                                                                                                <  2:135232/240‑1 (MQ=255)                                                                                                                       cggTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGCCGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGGTTACACCATTTTCAGGACGGGAAAAGGTggg                                                                                                                         >  1:363990/3‑249 (MQ=255)                                                                                                                       cggTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGCTTACACCATTCTCCGCACGGTACAAGGGGCg                                                                                                                         >  2:479274/3‑251 (MQ=255)                                                                                                                             aTCAGGATGTACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTTATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCtt                                                                                                                   <  2:220236/251‑1 (MQ=255)                                                                                                                             aTCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCtt                                                                                                                   <  1:337737/251‑1 (MQ=255)                                                                                                                              tCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGGAAAAGGTGCGGAGCTTg                                                                                                                  >  1:524652/1‑251 (MQ=255)                                                                                                                               ccggATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGAATCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTAAACCATTCACAGCACGGTAAAAAGGGCGGGGCTTg                                                                                                                  >  2:360699/3‑250 (MQ=255)                                                                                                                                aGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTTGTGATCTGGACCACTCA‑GCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACTTGATTTCGACTATGCGCTGGTGCAGAGCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCGCAGCACGGTAAAAGGTGCGGAGCTTG‑Ag                                                                                                               <  2:331542/251‑1 (MQ=255)                                                                                                                                           aaaGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCtt                                                                                                    <  1:231411/251‑1 (MQ=255)                                                                                                                                           aaaGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCtt                                                                                                    >  1:459263/1‑251 (MQ=255)                                                                                                                                              gCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑c                                                                                                >  1:638480/1‑251 (MQ=255)                                                                                                                                                 aaaTTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCTCCGGGCAGGCCGTGATTCTGGCGAAATATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTTACCGATTAAACCCTTCTCAACACGGTAAAATGAGCGGAGCTTG‑ATTTGCTGCGCTTTA‑ccc                                                                                              >  2:669952/1‑250 (MQ=255)                                                                                                                                                  aaTTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACGCCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCAt                                                                                            <  1:292442/251‑1 (MQ=255)                                                                                                                                                       cccGTAAGCAACGTTAACGGCCCAATCTCGCTGGGGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCGGATTTCGCCTATGCGCTGGTGCAGAGCGACGGTCAGGCCGTGATTCTGGCGAAAGAGCTGGTTGAAAGCGTAATTCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCAGCCGtt                                                                                       <  2:626852/250‑1 (MQ=255)                                                                                                                                                           aaaGCAACGTGAACGGCCCAATCTCGCTGGGGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCAGCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATg                                                                                   <  2:176596/250‑1 (MQ=255)                                                                                                                                                            aaGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATtc                                                                                                                                             <  2:136557/194‑1 (MQ=255)                                                                                                                                                            aaGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATtc                                                                                                                                             >  1:136557/1‑194 (MQ=255)                                                                                                                                                              gctaCGTGAACGGCCCAATCTCTCTGTTGATCTGGACCAC‑TACTCCGTGGACTCTGCCGTCGAACCGCGCAATCTCTATGGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGTCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATTCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCTGAGCTTg                                                                                                                  <  2:588961/216‑1 (MQ=255)                                                                                                                                                                   gTTAACGGCCCAATCCCGCTGGGGATCTGGACCAC‑TACGCCGTTGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGa                                                                           <  2:524652/251‑1 (MQ=255)                                                                                                                                                                     tAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGGAAAAGGGGCGGAGCTTG‑AGTGGCTGCGCTTTA‑CCCATCCGGTTAAGGGCTTCGACg                                                                         >  2:137896/1‑251 (MQ=255)                                                                                                                                                                        cGGCCCAATCTCGCTGGGTATCTGGACCCC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGGGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCAGTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTc                                                                      <  2:438627/251‑1 (MQ=255)                                                                                                                                                                                tctcGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGAACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGGTTATGGGCTTCGACGTTCCGGCAATc                                                              >  1:275842/1‑251 (MQ=255)                                                                                                                                                                                  tcGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCt                                                            >  1:288800/1‑251 (MQ=255)                                                                                                                                                                                           aTCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATc                                                   >  1:334561/1‑250 (MQ=255)                                                                                                                                                                                           aTCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTAACCGATTACAACATTCTCAGCACGGGAAAAGGGGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCCTCCGTTTTTGGGCTTCGACGTTACGGCAATACTCgggcattc                                                   >  2:83258/1‑245 (MQ=255)                                                                                                                                                                                                  ccac‑taCGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTAcc                                           >  1:339763/1‑251 (MQ=255)                                                                                                                                                                                                    cactacGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCATTCCCCGGCA‑ATCACGTTAccc                                          >  1:365022/5‑251 (MQ=255)                                                                                                                                                                                                     actacGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCt                                         >  1:343709/4‑251 (MQ=255)                                                                                                                                                                                                     actacGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGGCCGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGCTTACAAAATTCTCAGCACGGGACAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCACTCCGTTTATGGGCCTCGACGTTCCGACAATACTCGGCG‑ATCACGTAACCCt                                         >  2:624726/4‑251 (MQ=255)                                                                                                                                                                                                             gTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTTCACCATTCTCAGCACGGGTAAAGGTGCGGCGCTTG‑GGTTGCTGCGCTTTA‑CCCATCCGTTTCTGGGCTTAGTCGTTCCGGCAATCCTCGGCG‑ATCACGTTTCCCTggtttccg                                 >  2:286448/1‑245 (MQ=255)                                                                                                                                                                                                                    ctgcctgcGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTAcc                            <  2:104273/249‑1 (MQ=255)                                                                                                                                                                                                                    ctgcctgcGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTAcc                            <  1:83258/249‑1 (MQ=255)                                                                                                                                                                                                                       cctgcgAACCGCGCTAACTTTATTGAGCCTGATTTCGACTACGCGCTGGTGCAGATCGACGGTCAGGGCGTGATTCTGGCGAAAGATCTGGTTTACAGCGTAATGCAGCGTATCGGCGTGACCGATTACGCCATTCTCAGCACGGTAAAAGGTGCGGATCTTG‑AGTGGCTGCGCTTTC‑CCTGACCGTTTATTGGCGTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCttgacgtcggtctcggtgt                       <  2:449555/251‑19 (MQ=255)                                                                                                                                                                                                                        ctgcgAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGttt                                                                                      <  1:455757/188‑1 (MQ=255)                                                                                                                                                                                                                        ctgcgAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGAAAAAGGTGCGGGGCTTG‑GGTTGCTGCGCTTTA‑CCCATCCGttt                                                                                      >  2:455757/1‑188 (MQ=255)                                                                                                                                                                                                                            gaaCCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCAGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTc                  >  1:623105/2‑251 (MQ=255)                                                                                                                                                                                                                                cgcgAAATCTCTATTGCACCTGCTTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACAGATTACAACATTCTAAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CACATCCGTTTATGGGCTTCGACGATCCGGCAATACTCGGCG‑ATCACGTTACCCTGCATGACGGAAACGGTGCCGATCacac              >  1:111556/1‑251 (MQ=255)                                                                                                                                                                                                                                  cgcAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCCCAccc            >  1:315340/1‑250 (MQ=255)                                                                                                                                                                                                                                   gcAATCTCTATTGCACATGATTTCGACTATGCGCTGGCGCAGATCGACGGTCAGGCCGTGATTTTAGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATAACCACATTCACAGAACGGTAAAAGGCGCTGAGCTTGGATTTGC‑GCGCTTTAACCCCTC‑GCTTATTGGCTTCGACGTTACGGCAATCCACCGCGTACCA‑GTTACCCTGGATGCCGGTAccctgggcgttccacacac           >  2:308210/1‑234 (MQ=255)                                                                                                                                                                                                                                          ctATTGCACCTGATTTCGACGATGCGCTGGTGCAGATCGACGTGCAGGCCGTGATTCTGGCGAAAGAGCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCt       <  2:340027/248‑1 (MQ=255)                                                                                                                                                                                                                                            atgtcACCTGATTCCGATTATGCGCCGGTGCAGATCGACGGTCAGACCGGGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGTGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTG‑AGTTGCTGCTCTTTA‑CCCATCCGATTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGGGCCGTTCACACCGCGCCTGGCCa  <  2:31043/247‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      CACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCAC‑CACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTG‑AGCTGCTGCGCTTTA‑CCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCG‑ATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCA  >  NC_000913/22919‑23403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑