Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	23,104	A→G	V238V (GTA→GTG)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	23,104	0	A	G	94.9%	230.5 / 1.8	81	V238V (GTA→GTG)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (2/2);  new base G (44/30);  total (46/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.70e-01

GGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCAC‑CACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCT  >  NC_000913/22861‑23346                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    ggACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAAGCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGa                                                                                                                                                                                                                                              <  1:61258/251‑1 (MQ=255)    cTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCAGCACCGGACACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCAAATCTCGCTGGTGATCTGGacc                                                                                                                                                                                                                                            >  2:97422/1‑250 (MQ=255)      tCAAAGCTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGaccac                                                                                                                                                                                                                                          <  1:518852/250‑1 (MQ=255)         aaaCTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGCCACCTGCACAAAGGCGCGAAGACGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGACGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTGTCAGGCGGTCGATCCGAATTCACTGAAAGCAAAATTTGCCGTAAGCACCGTTAACGGCCCAATCTCCCTGGTGATATGGACCAC‑TACg                                                                                                                                                                                                                                     >  2:617388/1‑251 (MQ=255)            cTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCCACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGGTGAGTATTACGACAACACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATATGGacaactaagccg                                                                                                                                                                                                                                   >  2:242566/1‑241 (MQ=255)                  ccAACATCATCCGCGCGCTGGGCAAAATAATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTATGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGATGATCTGGCCCAC‑TACGCCGTGGAct                                                                                                                                                                                                                            >  1:125952/1‑251 (MQ=255)                       aacagccgctgtcTGGGCAAAATCATCGGCCACGGTCACCGTCACAAAGGCCCGAAGCCGGCACACTGGTGCGTGGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAACCTGCTCCGGCCATCGACGTTGCTTTTCAGGCGGTCGAACAGGATGCACTGAAAGCAAAATTTGCCGTAAGCACCGTTAACGGCCCCATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTctgcc                                                                                                                                                                                                                       <  1:29330/239‑1 (MQ=255)                         catcCGCGCGCGGCGCAAAATCATCGGCAACGGTCCCCTGCACAAAGGCGCGAAGCCAGTTCCCTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCGAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCAC‑CACGCCGTGGACTctgcctg                                                                                                                                                                                                                     <  2:280087/251‑1 (MQ=255)                             cgcgcgCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCAC‑CACGCCGTGGACTCTGCCTGCCa                                                                                                                                                                                                                  >  1:295310/1‑250 (MQ=255)                                gcgcTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACcg                                                                                                                                                                                                              <  1:344042/251‑1 (MQ=255)                                         acaaTCATCGGCAACGGTCACCTGCACAAAGGCGCGACGCCGGAACACTGGTGCGTTCACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACCCAACTTCACCGTCCATAGACGTTGCTTTTCAGGCGGTCGATCAGGATGTACTGAAAGCAAAACTTGCCGTAAGCAACGTTAACGGCCCACTCTCGCTGTCGATATGGACTAC‑CACACCCTGGACTCTGCCTGCGCACCGCGCCAtctc                                                                                                                                                                                                     >  2:287759/3‑251 (MQ=255)                                          aaaTCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGAAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATctct                                                                                                                                                                                                    >  1:488134/1‑251 (MQ=255)                                           aaTCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTa                                                                                                                                                                                                   <  1:97422/251‑1 (MQ=255)                                                           cACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCg                                                                                                                                                                                    >  1:487567/1‑250 (MQ=255)                                                            aCCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGAc                                                                                                                                                                                  <  1:53479/251‑1 (MQ=255)                                                             ccTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCACTAGCGGACGCGGAAGTTGAGTCTTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCCACCCGCGCCATCTCTATTGCACCTGCTTTCGACt                                                                                                                                                                                 >  1:421319/1‑251 (MQ=255)                                                                gCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAACATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATg                                                                                                                                                                              >  1:188603/1‑251 (MQ=255)                                                                gCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATg                                                                                                                                                                              >  1:138441/1‑251 (MQ=255)                                                                   caAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGATGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATgcgg                                                                                                                                                                           >  2:233262/1‑250 (MQ=255)                                                                     aaGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTg                                                                                                                                                                         >  1:612801/1‑251 (MQ=255)                                                                            gAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGCCGTTGAGTATTACGACCAAACTTCTCCGTCCATCGACGTTGCTTTTCCGGCGGTCGATCAGGATGCACTGCAAGCAAAATTTGCCGTAAGCAACGTTACCGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGAAATCTCTATTGCACCTGATTTCGACTAAGCGCTGGTGccgg                                                                                                                                                                  >  2:283476/1‑248 (MQ=255)                                                                            gAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCACCGTTAACGGCCCCATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGc                                                                                                                                                                     >  1:400745/1‑248 (MQ=255)                                                                             aaGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGAt                                                                                                                                                                 <  1:600369/251‑1 (MQ=255)                                                                                 cggtacACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGCGCTGATCGAc                                                                                                                                                             >  2:616229/6‑251 (MQ=255)                                                                                      cACTGGGGCGTTGCCGGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTCCAGGCAGTCGAACAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCAC‑CACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTc                                                                                                                                                         <  2:295310/250‑1 (MQ=255)                                                                                           gggcgtggccTGCCGTGCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTGCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCg                                                                                                                                                   <  2:3594/242‑1 (MQ=255)                                                                                              cGTTGACTGCCGCTCTGCGCCGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGGGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGa                                                                                                                                                <  1:193557/251‑1 (MQ=255)                                                                                                  gtcTGCCGATCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACGTCTCCGACCAGCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTGCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTc                                                                                                                                             <  1:5346/248‑1 (MQ=255)                                                                                                    cTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTgg                                                                                                                                          >  1:449555/1‑251 (MQ=255)                                                                                                         gTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGATAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACCCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCCCCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTTTggtcaa                                                                                                                                      >  2:333784/1‑246 (MQ=255)                                                                                                          ttCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGCACCGCGCAATCTCTATTGCCCCTGCTTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGaa                                                                                                                                      >  1:408925/1‑249 (MQ=255)                                                                                                                 cTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGt                                                                                                                             >  1:331542/1‑251 (MQ=255)                                                                                                                      ggaagcggaagTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAAGTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGaaa                                                                                                                        <  1:475199/251‑1 (MQ=255)                                                                                                                        aagcggaagTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGc                                                                                                                      >  1:104273/1‑251 (MQ=255)                                                                                                                            ggaagTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGCACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGGGCCGATCGACGGTCAGGCCGGGATTCTGGCGAAAGATCTg                                                                                                                               >  2:283003/1‑238 (MQ=255)                                                                                                                            ggaagTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTg                                                                                                                               <  1:283003/238‑1 (MQ=255)                                                                                                                                gTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGc                                                                                                               >  1:438627/1‑250 (MQ=255)                                                                                                                                  tGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCCATCTCGCTGGGGATCTGGACCAA‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATctct                                                                                                                                                                                                    >  2:539503/1‑163 (MQ=255)                                                                                                                                  tGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATctct                                                                                                                                                                                                    <  1:539503/163‑1 (MQ=255)                                                                                                                                  tGAGTATTACGAAAAAACTTCCCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTGCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAg                                                                                                             <  2:676222/250‑1 (MQ=255)                                                                                                                                     gTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGt                                                                                                          >  1:79123/1‑250 (MQ=255)                                                                                                                                        ttACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCg                                                                                                      >  1:166640/1‑251 (MQ=255)                                                                                                                                            gACAAAACTTCTCCGTCCATCGACGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCAC‑CACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGt                                                                                                  >  1:253931/1‑251 (MQ=255)                                                                                                                                             aCAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGAGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCg                                                                                                   >  1:430606/1‑249 (MQ=255)                                                                                                                                                aaaCTTCGCCGTCCACCGACTTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGTTGCAGAGCGACGGTCAGGCCGTGAGTCTGGCGAAAGAGCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAcc                                                                                              <  2:612801/251‑1 (MQ=255)                                                                                                                                                       tcCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTac                                                                                        >  1:288808/1‑250 (MQ=255)                                                                                                                                                        ccGTCCATCGCCGTTGCTTTTCCGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAAGCTCTCTGGTGATCTGGACCAC‑TACGCCGTGGACTCGGCCTGCGAACCGCGCAATCTCTATTGCACCTTATTTCGACTATGCGCTGGTGCAGATCGACGGGCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTacac                                                                                      <  2:381234/251‑1 (MQ=255)                                                                                                                                                        ccGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTa                                                                                         <  1:242566/248‑1 (MQ=255)                                                                                                                                                         cGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACAcc                                                                                     >  1:166281/1‑251 (MQ=255)                                                                                                                                                         cGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACAcc                                                                                     >  1:482421/1‑251 (MQ=255)                                                                                                                                                          gTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGCTTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGCTCTGGTTTAAAGCGGAATGCAGCGTATCGGCgaggaccgttaaaccc                                                                                    >  2:196756/1‑236 (MQ=255)                                                                                                                                                          gTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGTAGCGTATCGGCGTGAACGATTaaccc                                                                                     >  2:541079/1‑246 (MQ=255)                                                                                                                                                            ccATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCAtt                                                                                  <  1:236817/251‑1 (MQ=255)                                                                                                                                                             cATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGTCCGTGATTCTGGCGAAAGATCGGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATtc                                                                                 <  1:50283/251‑1 (MQ=255)                                                                                                                                                               ccGACGTTGCTTTTCAGGCCGTAGATCCGGAATCACTGAAATCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTTATCTGGACCAC‑TACGCCGTTGACTCTGCCTGCGAACCGTGCAATCTCTATTGCACCTGATTTCGTCTATGCGCTGGTGCAGATCGACGTTCAGGTTGTGATTCTGGCGAAATATCTGGTTGAAAGCGTAATGCATCGTATCGGCTTGTCCGATTACTCCATtctc                                                                               <  2:468469/250‑1 (MQ=255)                                                                                                                                                                  aCGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATtctcagc                                                                            <  1:495622/251‑4 (MQ=255)                                                                                                                                                                   cGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATtctcagc                                                                            >  1:59892/1‑247 (MQ=255)                                                                                                                                                                    gTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCAc                                                                          >  1:284427/1‑251 (MQ=255)                                                                                                                                                                           ttcaggcgagcGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGTCCCAATCTCGCTGGAGATCGGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGGCAGGCCGTGATTCTGGCGAAAGATCTTGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTaaa                                                                    <  2:135232/240‑1 (MQ=255)                                                                                                                                                                                 cggTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGCCGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGGTTACACCATTTTCAGGACGGGAAAAGGTggg                                                             >  1:363990/3‑249 (MQ=255)                                                                                                                                                                                 cggTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGCTTACACCATTCTCCGCACGGTACAAGGGGCg                                                             >  2:479274/3‑251 (MQ=255)                                                                                                                                                                                       aTCAGGATGTACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTTATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCtt                                                       <  2:220236/251‑1 (MQ=255)                                                                                                                                                                                       aTCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCtt                                                       <  1:337737/251‑1 (MQ=255)                                                                                                                                                                                        tCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGGAAAAGGTGCGGAGCTTg                                                      >  1:524652/1‑251 (MQ=255)                                                                                                                                                                                         ccggATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGAATCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTAAACCATTCACAGCACGGTAAAAAGGGCGGGGCTTg                                                      >  2:360699/3‑250 (MQ=255)                                                                                                                                                                                          aGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTTGTGATCTGGACCACTCA‑GCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACTTGATTTCGACTATGCGCTGGTGCAGAGCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCGCAGCACGGTAAAAGGTGCGGAGCTTGAg                                                    <  2:331542/251‑1 (MQ=255)                                                                                                                                                                                                     aaaGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCtt                                         >  1:459263/1‑251 (MQ=255)                                                                                                                                                                                                     aaaGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCtt                                         <  1:231411/251‑1 (MQ=255)                                                                                                                                                                                                        gCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTAc                                      >  1:638480/1‑251 (MQ=255)                                                                                                                                                                                                           aaaTTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCTCCGGGCAGGCCGTGATTCTGGCGAAATATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTTACCGATTAAACCCTTCTCAACACGGTAAAATGAGCGGAGCTTGATTTGCTGCGCTTTAccc                                    >  2:669952/1‑250 (MQ=255)                                                                                                                                                                                                            aaTTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACGCCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCAt                                  <  1:292442/251‑1 (MQ=255)                                                                                                                                                                                                                 cccGTAAGCAACGTTAACGGCCCAATCTCGCTGGGGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCGGATTTCGCCTATGCGCTGGTGCAGAGCGACGGTCAGGCCGTGATTCTGGCGAAAGAGCTGGTTGAAAGCGTAATTCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCAGCCGtt                             <  2:626852/250‑1 (MQ=255)                                                                                                                                                                                                                     aaaGCAACGTGAACGGCCCAATCTCGCTGGGGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCAGCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATg                         <  2:176596/250‑1 (MQ=255)                                                                                                                                                                                                                      aaGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATtc                                                                                 <  2:136557/194‑1 (MQ=255)                                                                                                                                                                                                                      aaGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATtc                                                                                 >  1:136557/1‑194 (MQ=255)                                                                                                                                                                                                                        gctaCGTGAACGGCCCAATCTCTCTGTTGATCTGGACCAC‑TACTCCGTGGACTCTGCCGTCGAACCGCGCAATCTCTATGGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGTCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATTCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCTGAGCTTg                                                      <  2:588961/216‑1 (MQ=255)                                                                                                                                                                                                                             gTTAACGGCCCAATCCCGCTGGGGATCTGGACCAC‑TACGCCGTTGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGa                 <  2:524652/251‑1 (MQ=255)                                                                                                                                                                                                                               tAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGGAAAAGGGGCGGAGCTTGAGTGGCTGCGCTTTACCCATCCGGTTAAGGGCTTCGACg               >  2:137896/1‑251 (MQ=255)                                                                                                                                                                                                                                  cGGCCCAATCTCGCTGGGTATCTGGACCCC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGGGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCAGTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTc            <  2:438627/251‑1 (MQ=255)                                                                                                                                                                                                                                          tctcGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGAACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGGTTATGGGCTTCGACGTTCCGGCAATc    >  1:275842/1‑251 (MQ=255)                                                                                                                                                                                                                                            tcGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCt  >  1:288800/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    GGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCAC‑CACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCT  >  NC_000913/22861‑23346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑