Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	22,945	T→A	V185V (GTT→GTA)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	22,945	0	T	A	93.8%	188.8 / ‑0.6	65	V185V (GTT→GTA)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (2/2);  new base A (37/23);  total (40/25)
Fisher's exact test for biased strand distribution p-value = 6.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.77e-01

AAGTCGAGCAAGAATACGGTAAGCCGGGTGAGAAATTCACCGCCGCCGAGTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTT‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGAT  >  NC_000913/22704‑23187                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                    aaGTCGAGCAAGAATACGGTAAGCCGGGTGAGAAATTCACCGCCGCCGAGTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGc                                                                                                                                                                                                                                           >  1:401310/1‑251 (MQ=255)   gTCGAGCAAGAATACGGTAAGCCGGGTGAGAAATTCACCGCCGCCGAGTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGt                                                                                                                                                                                                                                         >  1:381234/1‑251 (MQ=255)        gCAAGAATACGGTAAGCCGGGTGAGAAATTCACCGCCGCCGAGTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACt                                                                                                                                                                                                                                    <  1:338140/251‑1 (MQ=255)              aTACGGTAGGCCGGGGGAGAAATGACCCCCCGCCTAGTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGGTTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGtt                                                                                                                                                                                                                              <  2:572193/251‑1 (MQ=255)              aTACGGTAAGCCGGGTGAGAAATTCACCGCCGCCGAGTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAACCTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGGGCGTTGACTGCCGtt                                                                                                                                                                                                                              >  2:337737/1‑251 (MQ=255)                     aaGCCGGGTGAGAACTTCACCGCCGCCGAGTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGAAGGTCAACGCAGAGACTTTATCCGTCTGGGCGTGATGAGCGACTGGTCGCACCCGTAACTGACCATGGACTTCAAAACTGCAGCCAGCATCATCCGCGCGCTGGGCAAAATCATCGGCAAAGATCACCTGCACAAAGGCGCGCAACCGGTG‑ACCTGGTGCGTTGACTGCCGTTCTGCGCt                                                                                                                                                                                                                       >  2:518852/1‑251 (MQ=255)                       gCCGGGTGAGAAATTCACCGCCGCCGAGTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTgg                                                                                                                                                                                                                     >  1:626852/1‑251 (MQ=255)                            gTGAGAAATTCACCGCCGCCGAGTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCCCGGTTGCCGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCC‑GTTACCCTGGTGCGTTGTCTGCCGGTCTGAGCTGacgga                                                                                                                                                                                                                 >  2:344042/1‑250 (MQ=255)                            gTGAGAAATTCACCGCCGCCGAGTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGgcggaa                                                                                                                                                                                                                <  1:203341/251‑1 (MQ=255)                              gagaAATTCACCGCCGCCGAGTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGgcggaagc                                                                                                                                                                                                              >  1:230020/1‑251 (MQ=255)                              gagaAATTCACCGCCGCCGAGTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCAAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGgcggaagc                                                                                                                                                                                                              >  1:676222/1‑251 (MQ=255)                               agaAATTCACCGCCGCCGAGTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGgcggaagcg                                                                                                                                                                                                             >  1:31043/1‑251 (MQ=255)                                ccttccgatccccgccgAGTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGgcggaagcgg                                                                                                                                                                                                            <  2:311784/240‑1 (MQ=255)                                         cgccgccgAGTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGt                                                                                                                                                                                                   >  1:292514/1‑251 (MQ=255)                                           ccgccgAGTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGgcggaagcgg                                                                                                                                                                                                            >  1:311784/1‑240 (MQ=255)                                               cgAGTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGGTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACg                                                                                                                                                                                             <  1:216689/251‑1 (MQ=255)                                                  gTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTAAACGCAAAGCCTTTATCCGTCGGGTCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGGCTGCAAAACTGAAGCCAACATCACCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAACGGCGCGAAGCCGGTA‑CATTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACa                                                                                                                                                                                          <  2:249693/251‑1 (MQ=255)                                                   ttCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACa                                                                                                                                                                                          >  1:510985/1‑250 (MQ=255)                                                         gcCAAGTACCGCGAATACGCGTCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCAGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAACATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGATGACCTCCGTTCTGCCCTGGCAGAGGCGGCAGTTGCGTATTAGGACAAAACTtc                                                                                                                                                                                   >  2:45910/1‑251 (MQ=255)                                                          ccAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTTCCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTtct                                                                                                                                                                                  <  1:676752/251‑1 (MQ=255)                                                          ccAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTtct                                                                                                                                                                                  <  1:596832/251‑1 (MQ=255)                                                                   gcgAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATc                                                                                                                                                                         <  1:674610/251‑1 (MQ=255)                                                                    cgAATACGCGGCGACCCAGGTTGACGGTCAACGCTAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCg                                                                                                                                                                        <  1:65221/251‑1 (MQ=255)                                                                    cgAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGCAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCg                                                                                                                                                                        >  1:135232/1‑251 (MQ=255)                                                                                   ccaggtgtaCGGTCTACGCAAAGACTTCATCCGTCTGGGCGGGCTGGGCGACTGGTCTCACCCGTCCCTGACCATGGCCTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGGCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCCACGACGTTGCTTTTCAgg                                                                                                                                                         <  2:468000/243‑1 (MQ=255)                                                                                    cAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGAGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGc                                                                                                                                                        >  1:61936/1‑251 (MQ=255)                                                                                       gTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGAACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGAATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGcggt                                                                                                                                                     >  1:313443/1‑248 (MQ=255)                                                                                                 aaCGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGAt                                                                                                                                           >  1:340027/1‑251 (MQ=255)                                                                                                       aaGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTg                                                                                                                                     <  1:208830/251‑1 (MQ=255)                                                                                                                     cTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGc                                                                                                                       >  1:380799/1‑251 (MQ=255)                                                                                                                     cTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCAGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGc                                                                                                                       >  1:176596/1‑251 (MQ=255)                                                                                                                       gggCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCg                                                                                                                     >  1:220236/1‑251 (MQ=255)                                                                                                                           gTGCTGGGCGACTGGTCTCACCCGTACCTAACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCAACGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAg                                                                                                                 <  2:237830/251‑1 (MQ=255)                                                                                                                                               cccGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCGGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAAtct                                                                                             <  1:45910/251‑1 (MQ=255)                                                                                                                                                   tACCTGACCATGGACTACAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAACGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGGATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGTCAGTCGATCAGGATGCCCTGCAAGCAAAATTTGCCGTAAGCACCGTTAAAGGCACAATCTCGCt                                                                                         >  2:475199/1‑251 (MQ=255)                                                                                                                                                    ccctaaCCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCTAAATCATCGGCAACGGTCACCTGCACAAGGGGGCGAAGCC‑GTTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTg                                                                                        <  2:217414/246‑1 (MQ=255)                                                                                                                                                    aCCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGGCGATCAGGTTGCACTGAAAGCAAAATTTGCCGTTAGCACCGTTAACGGCCCAATCTCGCTg                                                                                        >  2:231411/1‑251 (MQ=255)                                                                                                                                                    aCCTAACCATGGACTCCAAAACTGAGGCAAACATCACCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTg                                                                                        <  2:292514/251‑1 (MQ=255)                                                                                                                                                         aCCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGATGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGAGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGAAGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCCAAATTAGCCGTAAGCAACGTTAACGGCCCCATCTCGCTGGtgat                                                                                   >  2:495622/1‑248 (MQ=255)                                                                                                                                                              ggACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAAGCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGa                                                                              <  1:61258/251‑1 (MQ=255)                                                                                                                                                                 cTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCAGCACCGGACACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCAAATCTCGCTGGTGATCTGGacc                                                                            >  2:97422/1‑250 (MQ=255)                                                                                                                                                                   tCAAAGCTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGaccac                                                                          <  1:518852/250‑1 (MQ=255)                                                                                                                                                                     aaaaCTGAAGCCAACATCATCCGCGCGCTGGGCATAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGTCTACCGTTCCGCGCTGGCGGAAGCGGAAGTTGAGTATTACGAAAAAACTTCTCCGTCCATCGCAGTTGCTTTTCAGGCGGTCGATCCGGATGCCCTGAAAACCAAATTTTCCATAAGCCAAGTTAACGGCCTCATCTCGCTgatggtctggcacactac                                                                       >  2:116373/1‑234 (MQ=255)                                                                                                                                                                      aaaCTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGCCACCTGCACAAAGGCGCGAAGACGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGACGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTGTCAGGCGGTCGATCCGAATTCACTGAAAGCAAAATTTGCCGTAAGCACCGTTAACGGCCCAATCTCCCTGGTGATATGGACCACTACg                                                                      >  2:617388/1‑251 (MQ=255)                                                                                                                                                                         cTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCCACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGGTGAGTATTACGACAACACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATATGGacaactaagccg                                                                   >  2:242566/1‑241 (MQ=255)                                                                                                                                                                           gAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGCCGTTGCTTTTCAGGCGGTCGATCAGGATGc                                                                                                                                         >  2:342838/1‑179 (MQ=255)                                                                                                                                                                           gAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGc                                                                                                                                         <  1:342838/179‑1 (MQ=255)                                                                                                                                                                               ccAACATCATCCGCGCGCTGGGCAAAATAATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTATGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGATGATCTGGCCCACTACGCCGTGGAct                                                             >  1:125952/1‑251 (MQ=255)                                                                                                                                                                                    aacagccgctgtcTGGGCAAAATCATCGGCCACGGTCACCGTCACAAAGGCCCGAAGCCGGCA‑CACTGGTGCGTGGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAACCTGCTCCGGCCATCGACGTTGCTTTTCAGGCGGTCGAACAGGATGCACTGAAAGCAAAATTTGCCGTAAGCACCGTTAACGGCCCCATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTctgcc                                                        <  1:29330/239‑1 (MQ=255)                                                                                                                                                                                      catcCGCGCGCGGCGCAAAATCATCGGCAACGGTCCCCTGCACAAAGGCGCGAAGCCAGTT‑CCCTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCGAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTctgcctg                                                      <  2:280087/251‑1 (MQ=255)                                                                                                                                                                                          cgcgcgCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTT‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCa                                                   >  1:295310/1‑250 (MQ=255)                                                                                                                                                                                             gcgcTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACcg                                               <  1:344042/251‑1 (MQ=255)                                                                                                                                                                                                      acaaTCATCGGCAACGGTCACCTGCACAAAGGCGCGACGCCGGAA‑CACTGGTGCGTTCACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACCCAACTTCACCGTCCATAGACGTTGCTTTTCAGGCGGTCGATCAGGATGTACTGAAAGCAAAACTTGCCGTAAGCAACGTTAACGGCCCACTCTCGCTGTCGATATGGACTACCACACCCTGGACTCTGCCTGCGCACCGCGCCAtctc                                      >  2:287759/3‑251 (MQ=255)                                                                                                                                                                                                       aaaTCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGAAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATctct                                     >  1:488134/1‑251 (MQ=255)                                                                                                                                                                                                        aaTCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTa                                    <  1:97422/251‑1 (MQ=255)                                                                                                                                                                                                                        cACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCg                     >  1:487567/1‑250 (MQ=255)                                                                                                                                                                                                                         aCCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGAc                   <  1:53479/251‑1 (MQ=255)                                                                                                                                                                                                                          ccTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCACTAGCGGACGCGGAAGTTGAGTCTTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCCACCCGCGCCATCTCTATTGCACCTGCTTTCGACt                  >  1:421319/1‑251 (MQ=255)                                                                                                                                                                                                                             gCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAACATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATg               >  1:188603/1‑251 (MQ=255)                                                                                                                                                                                                                             gCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATg               >  1:138441/1‑251 (MQ=255)                                                                                                                                                                                                                                caAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGATGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATgcgg            >  2:233262/1‑250 (MQ=255)                                                                                                                                                                                                                                  aaGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTg          >  1:612801/1‑251 (MQ=255)                                                                                                                                                                                                                                         gAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGCCGTTGAGTATTACGACCAAACTTCTCCGTCCATCGACGTTGCTTTTCCGGCGGTCGATCAGGATGCACTGCAAGCAAAATTTGCCGTAAGCAACGTTACCGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGAAATCTCTATTGCACCTGATTTCGACTAAGCGCTGGTGccgg   >  2:283476/1‑248 (MQ=255)                                                                                                                                                                                                                                         gAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCACCGTTAACGGCCCCATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGc      >  1:400745/1‑248 (MQ=255)                                                                                                                                                                                                                                          aaGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGAt  <  1:600369/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                    AAGTCGAGCAAGAATACGGTAAGCCGGGTGAGAAATTCACCGCCGCCGAGTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTT‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGAT  >  NC_000913/22704‑23187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑