| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 21,952 | G→A | E182E (GAG→GAA) | ribF → | bifunctional riboflavin kinase/FMN adenylyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 21,952 | 0 | G | A | 98.2% | 174.7 / ‑4.4 | 57 | E182E (GAG→GAA) | ribF | bifunctional riboflavin kinase/FMN adenylyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (20/35); total (21/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.93e-01 | |||||||||||
GTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCATTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGCATCAGCAGCACCGCCGTGCGTCAGGCCCTTGCGGATGACA‑ATCTGGCTCTGGCAGAGAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTG‑CGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAAACATCGGAACACGCCCAACGGTTGCCGGTATTCGCCAGCAGCTGGAAGTGCATTTGTTAGATG > NC_000913/21709‑22193 | gTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTAc < 1:248967/251‑1 (MQ=255) cgcAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCAc > 1:620492/1‑251 (MQ=255) aaaaTTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCg < 1:493111/251‑1 (MQ=255) tcatcaGCGATCTTCTGGTGCCGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGACGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGCAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATG‑CACATCTGGCTCCGGCAGAAAG‑GTTACTGGGGCACCCGTTTGcc > 2:669245/1‑251 (MQ=255) aGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCAtctc < 1:456149/251‑1 (MQ=255) aGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAAATTCTTGCCGTAGGTGATGCTTTCAGATTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACAAGTACGCAAACTTTTTGCGAAGGTGGCGAGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAGGTTTA‑TGGGGCACCCGTTTGCCAtctt > 2:564078/1‑250 (MQ=255) gCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGACAG‑TTTACTGGGGCACCCGTTTGCAATCTcc > 2:368676/1‑251 (MQ=255) ttgTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGTTGATGATTTCCGCTTTGGCGCTTGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGTAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGTCGTGCTTATCAGCATCACCGCTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTAc < 1:381067/217‑1 (MQ=255) ggTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCgtg < 1:459223/248‑1 (MQ=255) cgcttgtGCGCGTAAAATTTCTTGCCGTATGTGATGGTTTCCGTTTTGGCGCTGGGCGGGAAGGCGTTTTCTTGTTATTGCAGAAAGCTGGCATTTACTTCGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGTGTATCAGCAGCACCGCTGTGTGTCAGGCGCTGGCGGATGTCA‑ATCTGGCTCTGGTAGAAAG‑TTTACTGTGGCACCCGTTTTCCATCTCCGGGCGTGTAGTCCa < 2:147778/244‑1 (MQ=255) tttGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGt < 1:91699/250‑1 (MQ=255) gttGCGCGTAAAATTTCTGGCCGTAGGGGATTATTTCCGCTTTGGCGCTGGTCGTGAAGGAGATTTCTTGTTATTACAGAAAGCTGGCAGGGAATACGGCTTCGATAGCACCAGTACGTAATCTTTTTGCGAAGGTGGCGTGCGCATCAGCAGCACCGCCGTGCGTCAGGCCCTTGCGGATGACA‑ATCTGGCTCTGGCAGAGAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACgg < 2:583452/248‑1 (MQ=255) gcgcGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGtgat < 1:199641/250‑1 (MQ=255) cgTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCGCCGGGCGTGTAGTCCACGGTGATGAAt < 1:267595/251‑1 (MQ=255) tGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCa < 1:522104/248‑1 (MQ=255) tGCCGTAGGTGATGATTTCCGCTTGGGCGCTGGTCGTGATGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATAGCACCATTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTTGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCAc < 2:366516/249‑1 (MQ=255) ccGTAGGTGATGATTTCCGCTTTAGCGATGGTCGTGAAGGCGATTTCTTGTTATTACAGAAACCTGGCATGGGATACGGCTTCGATATGACCAGGACGCAAACTTTTTGCGACGGTGGAGTGCGTCTCAGCAGCACCGCTGTGCGTCAGGCGCATGAGGATGACA‑ATCTGGATATGGCAGTAAG‑TTTAATGGCGCACCCGATTGACATCTCCGGGCGTGTAGTACGAGGTGATGAGTTAGGACGCACtat > 2:660262/1‑250 (MQ=255) aGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGtt < 1:477049/251‑1 (MQ=255) ggaggatTTCCGGTGTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATAGCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGATA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCTGGCGTATAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTcc < 1:286718/247‑1 (MQ=255) cGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGaa < 1:652962/251‑1 (MQ=255) tgggcGTGAAGGCGATTTCTTGTTATTACAGAAAGCCGGCATGGAAAACCGCCTCGATATCCCCAGGACGCAAACTTTTTGCGAAGGTGGCGTGCGGAACAGCAGCACCGCTGGGCGGCAGGCGGTGGGCGATGGAA‑ATCTGGCCCTGGGAGAAAG‑GTTAATGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGtg < 1:282181/199‑1 (MQ=255) gtgTCGTGAAGGCGATTTCTCTTTAGTACAGAAAGCTGGCATGGAATACGTCTGCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTTGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCTCGGTGATGAATTAGTGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCtac < 2:152095/249‑3 (MQ=255) ggTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTTGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGGCCCCGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCtacg > 2:246090/1‑248 (MQ=255) cGATTTCTTTTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCG‑TGACGCCGTCAGGttt < 2:620492/251‑1 (MQ=255) cGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCCGCACCGCTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑GTTACTGGGGCACCCGGTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAAGGAGCACTATAGGTTTCCCGCCGCCGAATGTCCCGGTA‑CGCCGTCAGGttt > 2:228810/1‑251 (MQ=255) tGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTA‑CGCCGTCAGGTTTCCCCGGt < 1:336632/250‑1 (MQ=255) gAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTA‑CGCCGTCAGGTTTCCCCGGTGAAAGGGGttt < 1:330251/251‑1 (MQ=255) cTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAACG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGAACTATAGGTTTCCCGACGGCGCATGTACCGCTA‑CGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCg > 2:649648/1‑251 (MQ=255) ggAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTA‑CGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGaa < 1:409839/250‑1 (MQ=255) ggCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTCGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTA‑CGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGGAGAAGTGCTggg > 1:445395/1‑251 (MQ=255) cGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTA‑CGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTc < 1:12132/250‑1 (MQ=255) gATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTA‑CGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCgg > 1:450743/1‑251 (MQ=255) atatCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTA‑CGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGt < 1:256701/251‑1 (MQ=255) atatCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTA‑CGCCGTCAGGTTTCCCCGGTGAAAGGGGATTATGCGGTAGAAGTGCTGGGCCTCGGt > 1:347301/1‑251 (MQ=255) atCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGTGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTA‑CGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTg < 1:254638/250‑1 (MQ=255) cACCAGTACGCAAACTCGTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTTCCGACGGCGAATGTACCGCTA‑CGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGaa < 1:318998/250‑1 (MQ=255) aCTTTTTGCGAAGGAGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTA‑CGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATTCGGTAGAAGTGCTGGGGCTCGGTGAAAAGCCGTTACCCgg > 2:366588/1‑251 (MQ=255) cTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTA‑CGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGc > 1:45572/1‑251 (MQ=255) ggTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTA‑CGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAAACAt < 1:564078/251‑1 (MQ=255) gctggcggacccgcatcaCCGCTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGTAGAAAG‑TTTACTGGGGGTCCCGTTTGCCATCTCCGGGCGGGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTA‑CGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTTCCCGGCGTGGCAAACATCgta < 2:654642/235‑3 (MQ=255) gtaTCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTA‑CGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAAACATCGGAACACGc > 1:676968/3‑251 (MQ=255) tCAGCAGCGCCGCGGGGCGTCAGGCGCTTGGGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCTTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTA‑CGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAAACATCGGAACACGccc < 2:267532/250‑1 (MQ=255) cagcagCACCGCTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTA‑CGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCGCGGTGAAAAGCCGTTACCCGGCGTGGCAAACATCGGAACACGCCCaa < 1:628227/251‑1 (MQ=255) gcaCCGCTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTA‑CGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAAACATCGGAACACGCCCAACGGtt < 1:177250/251‑1 (MQ=255) ctgTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTA‑CGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAAACATCGGAACACGCCCAACGGTTGCCGGt > 1:310859/3‑251 (MQ=255) gTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTA‑CGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAAACATCGGAACACGCCCAACGGTTGCCGGTATTCGc > 1:294274/1‑251 (MQ=255) tcaggctctggaggATAACA‑AACTGGCTCTGGCCGAAAG‑CTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGCATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTA‑CGCCGTCAGGTTTCCCCGGTGAACGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGCGGACAGCCGTTACCCGGCGTGGCAAACATCGGACCACGCCCCAAGGTTGCCGGTATTCGcc > 2:572442/13‑251 (MQ=255) caggcgcgggCGGATGACA‑ATCGGGCCTTGGCATAAAG‑TCTCCTGGGGCAACCTTTTGTCATCTCCGGGCGTGTAGTCCACGGTGATGAATGAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTA‑CGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAAACATCGGAACACGCCCAACGGTTGCCGGTATGCGCca < 2:445395/242‑1 (MQ=255) cgctggcggaggacatgctggcgctggcAGAAAG‑TTTACTGGGGCACCCGTGTGCAGTCTGCGGGCGGGTAGTCCACGGTGATGAATTAGGGGGCACTAGAGGTTTCCCGACGGTGAATGTTCCGCTG‑CGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAAACATCGGAACACGCCCAACGGTTGCCGGTATTCGCcagcag < 2:311823/234‑1 (MQ=255) ggCGGATGTCA‑ATCAGGCTCTGGCAGAAAG‑TTTCCGGGGGCACCCGTTTGCCATCGCCGGGGGTGTAGGCCACGGGGATGAATTAGGGCGCACTATAGGGTTCCCGGCGGTGAATGTACCGCTA‑CGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAAACATCGGAACACGCCCAACGGTTGCCGGTATTCGCCAGCAGCTg < 2:525268/249‑1 (MQ=255) ggCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTA‑CGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAAACATCGGAACACGCCCAACGGTTGCCGGTATTCGCCAGCAGCTgg < 1:649648/250‑1 (MQ=255) ggATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGACTGTACCGCTA‑CGCCGTCAGGTTTCCCCGGTGAAAGGGGGTTATGCGGTAGAAGTGCTGGGCCTCGGTGAACAGCCGTTACCCGGCGTGGCAAACATCGGCACACGCCCAACGGTTGCCGGTATTCGCCAGCAGCTGGa > 2:635632/1‑249 (MQ=255) aTGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTA‑CGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTTCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAAACATCGGAACACGCCCAACGGTTGCCGGTATTCGCCAGCAGCTGGAAGTg > 1:535648/1‑251 (MQ=255) tGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTA‑CGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAAACATCGGAACACGCCCAACGGTTGCCGGTATTCGCCAGCAGCTGGAAGTGc > 1:181062/1‑251 (MQ=255) aTCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTA‑CGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAAACATCGGAACACGCCCAACGGTTGCCGGTATTCGCCAGCAGCTGGAAGTGCATTTg > 2:587435/1‑251 (MQ=255) ggctctggcAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTA‑CGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAAACATCGGAACACGCCCAACGGTTGCCGGTATTCGCCAGCAGCTGGAAGTGCATTTGTTa < 1:246090/250‑1 (MQ=255) ggcAGAAAG‑CTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTTATGAATTAGGGCGCACTATATGTTTCCCGACGGCGAATGTACCGCTA‑CGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGAAGAAGTGCTGGGCCTCGGTGAAAATCCGTTTCCCGGCGTGGCAAACATCGGAACACGCCCAACGGTTGCCGGTATTCGCCAGCAGCTGGAAGTGCATTTGTTAGATg < 1:406916/248‑1 (MQ=255) | GTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCATTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGCATCAGCAGCACCGCCGTGCGTCAGGCCCTTGCGGATGACA‑ATCTGGCTCTGGCAGAGAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTG‑CGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAAACATCGGAACACGCCCAACGGTTGCCGGTATTCGCCAGCAGCTGGAAGTGCATTTGTTAGATG > NC_000913/21709‑22193 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |