| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 21,895 | C→T | R163R (CGC→CGT) | ribF → | bifunctional riboflavin kinase/FMN adenylyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 21,895 | 0 | C | T | 96.2% | 150.2 / ‑2.6 | 54 | R163R (CGC→CGT) | ribF | bifunctional riboflavin kinase/FMN adenylyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (18/33); total (19/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.79e-01 | |||||||||||
GCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCATTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGCATCAGCAGCACCG‑CCGTGCGTCAGGCCCTTGCGGATGACA‑ATCTGGCTCTGGCAGAGAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTG‑CGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAAACAT > NC_000913/21653‑22134 | gCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAACGCTGGCATGGAGTACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGAGCGTATcagc > 2:235772/1‑249 (MQ=255) ggtgtGGCGTTGATTACGTGCTGGGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCAcc < 2:142951/250‑1 (MQ=255) aTTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCG‑CTGTGCGTCAg < 1:533149/251‑1 (MQ=255) ttACGTGCTGTGCGTGCGTTTCGACAGGCGATTAGCGGCGTTAACCGCGCAAACTTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTCGGTGATGATTTCCGCTTTGGCGCTGGTCGCGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAACCTTTTTGCGAAGGTGGCGTGCGTATCAGCAG‑ACCGGCCATGCGTCAgg > 2:278723/1‑251 (MQ=255) tgtgCGTGCGTTTCGACAGGCATTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGGGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGGGCTGATTTCCGCTTTGGCGCTGGTCGTGTAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCAATATAACCATAACGCAAACTTTTAGCGAAGGTGGCGAGGGTATCTGCAGCAACG‑CTGTGCGTCAGGCCCtgg > 2:21186/1‑247 (MQ=255) cgggcgtTTCGACAGGCGTTACGGGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGGAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCG‑CTGTGCGTCAGGCGCTGGCGGATg < 2:547024/248‑1 (MQ=255) gtgcgtTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCG‑CTGTGCGTCAGGCGCTGGCGGATGa < 1:399019/251‑1 (MQ=255) tgcgtTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGCTCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATACGCAGCACCG‑CTGTGCGTCAGGcgcgggcggctgc > 2:336632/1‑238 (MQ=255) ttctaCAGGCGTTTCGCGGCGTTATCCGCGCAAAATTTCATCAGCGAGCTTCTGGTGAAGCTTTTGCGCGTAAAATTGCTTGCCGTAGGTGATGATTTCCTCTTTGGCGCTTGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCT‑CTGTGCGTCAGGCGCTGGCGGATGACA‑Atc < 1:121164/246‑1 (MQ=255) ttcgcaaGGCGTGTCGCGGCGTTAATCGCGCAAAATTTCATCAGCGATTTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCG‑CTGTGCGTCAGGCGCTGGCGGATGACA‑Atc < 2:798/244‑1 (MQ=255) cGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCATTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGCATCAGCAGCACCG‑CCGTGCGTCAGGCCCTTGCGGATGACA‑Atctgg > 1:583452/1‑251 (MQ=255) aGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCG‑CTGTGCGTCAGGCGCTGGCGGATGACA‑Atctggctct < 1:133264/251‑1 (MQ=255) gcggcgTTAACCGCGCAAAATGTCAGCAGCGATCTTCTGGTGAAGCTTTTGCGTTTAAAAGTTCTTGACGTAGGTGATGATGTTCGCTTTGGCGCTGGTCGTGAAGGCGAGTTCTTGTTATTACAGAAAGCTGGCATGGAATACTGCTTCGATATCACCAGTACGCAAACTTTTGGCTAAGGTGGCGGGCGTAGCAGCAGCACCG‑CTGTGCGTCAGGCTCTTGGGGATGACA‑AGCTGGCTCTGGCagaaag < 2:677287/251‑4 (MQ=255) gTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCG‑CTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTAc < 1:248967/251‑1 (MQ=255) cgcAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCG‑CTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCAc > 1:620492/1‑251 (MQ=255) aaaaTTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCG‑CTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCg < 1:493111/251‑1 (MQ=255) tcatcaGCGATCTTCTGGTGCCGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGACGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGCAGGTGGCGTGCGTATCAGCAGCACCG‑CTGTGCGTCAGGCGCTGGCGGATG‑CACATCTGGCTCCGGCAGAAAG‑GTTACTGGGGCACCCGTTTGcc > 2:669245/1‑251 (MQ=255) caGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATcagcag > 2:19219/1‑174 (MQ=255) caGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATcagcag < 1:19219/174‑1 (MQ=255) aGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCG‑CTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCAtctc < 1:456149/251‑1 (MQ=255) aGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAAATTCTTGCCGTAGGTGATGCTTTCAGATTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACAAGTACGCAAACTTTTTGCGAAGGTGGCGAGCGTATCAGCAGCACCG‑CTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAGGTTTA‑TGGGGCACCCGTTTGCCAtctt > 2:564078/1‑250 (MQ=255) gCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCG‑CTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGACAG‑TTTACTGGGGCACCCGTTTGCAATCTcc > 2:368676/1‑251 (MQ=255) ttgTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGTTGATGATTTCCGCTTTGGCGCTTGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGTAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGTCGTGCTTATCAGCATCACCG‑CTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTAc < 1:381067/217‑1 (MQ=255) ggTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCG‑CTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCgtg < 1:459223/248‑1 (MQ=255) cgcttgtGCGCGTAAAATTTCTTGCCGTATGTGATGGTTTCCGTTTTGGCGCTGGGCGGGAAGGCGTTTTCTTGTTATTGCAGAAAGCTGGCATTTACTTCGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGTGTATCAGCAGCACCG‑CTGTGTGTCAGGCGCTGGCGGATGTCA‑ATCTGGCTCTGGTAGAAAG‑TTTACTGTGGCACCCGTTTTCCATCTCCGGGCGTGTAGTCCa < 2:147778/244‑1 (MQ=255) tttGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCG‑CTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGt < 1:91699/250‑1 (MQ=255) gttGCGCGTAAAATTTCTGGCCGTAGGGGATTATTTCCGCTTTGGCGCTGGTCGTGAAGGAGATTTCTTGTTATTACAGAAAGCTGGCAGGGAATACGGCTTCGATAGCACCAGTACGTAATCTTTTTGCGAAGGTGGCGTGCGCATCAGCAGCACCG‑CCGTGCGTCAGGCCCTTGCGGATGACA‑ATCTGGCTCTGGCAGAGAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACgg < 2:583452/248‑1 (MQ=255) gcgcGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCG‑CTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGtgat < 1:199641/250‑1 (MQ=255) cgTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCG‑CTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCGCCGGGCGTGTAGTCCACGGTGATGAAt < 1:267595/251‑1 (MQ=255) tGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCG‑CTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCa < 1:522104/248‑1 (MQ=255) tGCCGTAGGTGATGATTTCCGCTTGGGCGCTGGTCGTGATGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATAGCACCATTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCG‑CTGTGCGTCAGGCGCTTGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCAc < 2:366516/249‑1 (MQ=255) ccGTAGGTGATGATTTCCGCTTTAGCGATGGTCGTGAAGGCGATTTCTTGTTATTACAGAAACCTGGCATGGGATACGGCTTCGATATGACCAGGACGCAAACTTTTTGCGACGGTGGAGTGCGTCTCAGCAGCACCG‑CTGTGCGTCAGGCGCATGAGGATGACA‑ATCTGGATATGGCAGTAAG‑TTTAATGGCGCACCCGATTGACATCTCCGGGCGTGTAGTACGAGGTGATGAGTTAGGACGCACtat > 2:660262/1‑250 (MQ=255) aGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCG‑CTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGtt < 1:477049/251‑1 (MQ=255) ggaggatTTCCGGTGTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATAGCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCG‑CTGTGCGTCAGGCGCTGGCGGATGATA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCTGGCGTATAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTcc < 1:286718/247‑1 (MQ=255) cGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCG‑CTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGaa < 1:652962/251‑1 (MQ=255) tgggcGTGAAGGCGATTTCTTGTTATTACAGAAAGCCGGCATGGAAAACCGCCTCGATATCCCCAGGACGCAAACTTTTTGCGAAGGTGGCGTGCGGAACAGCAGCACCG‑CTGGGCGGCAGGCGGTGGGCGATGGAA‑ATCTGGCCCTGGGAGAAAG‑GTTAATGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGtg < 1:282181/199‑1 (MQ=255) gtgTCGTGAAGGCGATTTCTCTTTAGTACAGAAAGCTGGCATGGAATACGTCTGCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCG‑CTGTGCGTCAGGCGCTTGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCTCGGTGATGAATTAGTGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCtac < 2:152095/249‑3 (MQ=255) ggTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCG‑CTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTTGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGGCCCCGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCtacg > 2:246090/1‑248 (MQ=255) cGATTTCTTTTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCG‑CTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCG‑TGACGCCGTCAGGttt < 2:620492/251‑1 (MQ=255) cGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCCGCACCG‑CTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑GTTACTGGGGCACCCGGTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAAGGAGCACTATAGGTTTCCCGCCGCCGAATGTCCCGGTA‑CGCCGTCAGGttt > 2:228810/1‑251 (MQ=255) tGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCG‑CTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTA‑CGCCGTCAGGTTTCCCCGGt < 1:336632/250‑1 (MQ=255) gAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCG‑CTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTA‑CGCCGTCAGGTTTCCCCGGTGAAAGGGGttt < 1:330251/251‑1 (MQ=255) cTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCG‑CTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAACG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGAACTATAGGTTTCCCGACGGCGCATGTACCGCTA‑CGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCg > 2:649648/1‑251 (MQ=255) ggAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCG‑CTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTA‑CGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGaa < 1:409839/250‑1 (MQ=255) ggCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCG‑CTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTCGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTA‑CGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGGAGAAGTGCTggg > 1:445395/1‑251 (MQ=255) cGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCG‑CTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTA‑CGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTc < 1:12132/250‑1 (MQ=255) gATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCG‑CTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTA‑CGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCgg > 1:450743/1‑251 (MQ=255) atatCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCG‑CTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTA‑CGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGt < 1:256701/251‑1 (MQ=255) atatCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCG‑CTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTA‑CGCCGTCAGGTTTCCCCGGTGAAAGGGGATTATGCGGTAGAAGTGCTGGGCCTCGGt > 1:347301/1‑251 (MQ=255) atCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCG‑CTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGTGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTA‑CGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTg < 1:254638/250‑1 (MQ=255) cACCAGTACGCAAACTCGTTGCGAAGGTGGCGTGCGTATCAGCAGCACCG‑CTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTTCCGACGGCGAATGTACCGCTA‑CGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGaa < 1:318998/250‑1 (MQ=255) aCTTTTTGCGAAGGAGGCGTGCGTATCAGCAGCACCG‑CTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTA‑CGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATTCGGTAGAAGTGCTGGGGCTCGGTGAAAAGCCGTTACCCgg > 2:366588/1‑251 (MQ=255) cTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCG‑CTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTA‑CGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGc > 1:45572/1‑251 (MQ=255) ggTGGCGTGCGTATCAGCAGCACCG‑CTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTA‑CGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAAACAt < 1:564078/251‑1 (MQ=255) | GCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCATTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGCATCAGCAGCACCG‑CCGTGCGTCAGGCCCTTGCGGATGACA‑ATCTGGCTCTGGCAGAGAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTG‑CGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAAACAT > NC_000913/21653‑22134 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |