| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 21,750 | A→T | H115L (CAT→CTT) | ribF → | bifunctional riboflavin kinase/FMN adenylyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 21,750 | 0 | A | T | 97.3% | 224.0 / ‑3.4 | 74 | H115L (CAT→CTT) | ribF | bifunctional riboflavin kinase/FMN adenylyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base T (34/37); total (35/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.00e-01 | |||||||||||
CTGTTACAGGGCTTGCAGGAAGAAGGGCGCAAGCGCAACTTACCGGTGATGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCAAGACTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGG‑CGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCATTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGCATCAGCAGCACCG‑CCGTGCGTCAGGCCCTTGCGGATGACA‑ATCTGGCTCTGGCAGAGAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTG > NC_000913/21509‑21989 | cTGTTACAGGGCTTGCAGGAAGAAGGGCGCAAGCGCAACTTACCGGTGATGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGG‑CGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGt > 1:130454/1‑251 (MQ=255) tGTTACAGGGCTTGCAGGAAGAAGGGCGCAAGCGCAACTTACCGGTGATGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGG‑CGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGt > 1:525268/1‑250 (MQ=255) tcAGGGCTGGCAGGAAGAAGGTCGCAAGCGCAATGTTCCGGTGCTGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCCACCGCTAAAGCCCCGGCACGGCTGACCCGTCTGCGGGAAAATCTGCGTTACCTTGCAGAGGGTGG‑CGTTGATTACGGGCTGTGCGTGCGTTTCGACAGGTTTGTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTGCTGGTGAAGCGTTTGCGTGTaaaa < 2:406787/249‑1 (MQ=255) aCAGGGCTTGCAGGAATAATGGCGCAAGAGCAACTTTCCGGTGATGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGG‑CGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTaaaa < 2:590253/250‑1 (MQ=255) tGCAGGAAGAAAGGCGCAAGCGCAACTTACCGGTGATGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGG‑CGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGcc > 1:152095/1‑251 (MQ=255) gaagaaGGGCGCAAGCGCAACTTACCGGTGATGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGG‑CGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAgg < 1:592882/251‑1 (MQ=255) gcgcaagcgcaaCTTACCGGTGATGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGG‑CGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGACGCTTTTGCGCGTAAATTTTCTTGCCGTGGGTGATGAtt > 2:248967/1‑251 (MQ=255) aagcgaaaCTGACCGTTGATGGTGATGCTTTCTGAACCACAACCACGGGAACTGTTTGCGACCGATCAAGACCCGGAACGGCTGACCGGGCGGCGGGAAAAACTGCGTTACCTTGTATAGTGTGG‑CGGTGATTACGTGCTGGTCGTGCGCTGCGATAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCACCATTGATCTTCTGGTGAAGCTTTTGCGCTTAAAATTTCTCGCCTTAGTTGATGAtttgcg < 2:76007/251‑4 (MQ=255) cgcaaCGTACCGGTTATGGTGATGCTGTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGG‑CGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAAGTTCTTGCCGTAGGTGATGATTTCCGCtt < 1:465965/251‑1 (MQ=255) gcaaCTTACCGGTGATGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTAACTTGCAGAGTGTGG‑CGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGAGATGATTTCCGCttt > 1:654642/1‑251 (MQ=255) aCCGGTGATGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGG‑CGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTg < 1:562791/251‑1 (MQ=255) ggtgatgCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGG‑CGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGaa < 1:5269/250‑1 (MQ=255) gatgCTTTGTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACCGCGTTACCTTGCAGAGTGTGG‑CGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGc < 1:199722/250‑1 (MQ=255) tttGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACTGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGG‑CGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCt < 1:307769/250‑1 (MQ=255) aaCCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCACGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGG‑CGTTGATTACGTGCTGTGAGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGCTCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGTTGATGATTTCCTCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGtt > 2:254063/1‑250 (MQ=255) aaCCACTGGACCTGTTTGCTACCGATAAAGCCCCGGCCCGGCTGACCCGGTTGTGGGAAAAACTGCGTTACCTTGCAGAGTGTGG‑CGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAg < 2:448358/251‑1 (MQ=255) ggAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAACCTGCGTTACCTTGCAGAGTGTGG‑CGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGAGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGAAAAATTTCTTGCCGTAGGGGATGATTTCCGCTTTGGAGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTg > 1:617233/1‑251 (MQ=255) gCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGG‑CGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGCTCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTACGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGctgggaatc > 2:177250/1‑243 (MQ=255) cTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGG‑CGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATAc > 2:133264/1‑251 (MQ=255) aCCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTTCCTTGCAGAGTGTGG‑CGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATAc < 2:263403/249‑1 (MQ=255) aCCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGG‑CGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTGCAGCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACgg < 2:419024/251‑1 (MQ=255) ccGATAAAGCCCCGGCACGGCTGGCCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGG‑CGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGc < 2:17257/251‑1 (MQ=255) cGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGG‑CGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCt > 1:142951/1‑251 (MQ=255) aaGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGG‑CGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGCTCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAACTGGCATGGAATACGGCttgg > 2:207326/1‑247 (MQ=255) aGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGG‑CGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGata < 2:261268/251‑1 (MQ=255) aGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGG‑CGGTGATTACGGGCTGTGCGTGCGGTTCGACAGGCGTTTCGAGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGata < 1:611378/251‑1 (MQ=255) gCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGG‑CGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGatat > 1:366516/1‑251 (MQ=255) gCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGG‑CGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGatat < 1:644009/251‑1 (MQ=255) gCCCCGGCACGGATGACCCGGCTGCGGGAAAAACTGAGTTACCTTGCAGAGTGTGG‑CGATGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCTCAAAATTTCATCAGCGATCTTATGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATAATTTCCGCTTTGGCGCTGTGCGTGAAGCCGATTTCTAGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGatct > 2:533149/1‑249 (MQ=255) acggcTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGG‑CGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCttt < 1:162505/174‑1 (MQ=255) acggcTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGG‑CGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCttt > 2:162505/5‑178 (MQ=255) acggcTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGG‑CGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGt > 1:267532/5‑250 (MQ=255) tGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGG‑CGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCa > 1:188533/1‑250 (MQ=255) gACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGG‑CGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCaa > 1:167935/1‑250 (MQ=255) gACCCGGCTGCGGGAAAAACTGCGTGTCCTTGCAGAGTGGGG‑CGTTGATTACGTGCTGTGCTTGCGTTGCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAAGTTCATCAGCGATCTTCTGGTGAAGCATTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCTATTTCTTGTTATTACAGAAAGCTGGCATGGAATTCGGCTTCGATATCACCAGTACGCa < 2:548039/249‑1 (MQ=255) ggCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGG‑CGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACtttt > 1:798/1‑251 (MQ=255) gctggAAAATCTGCGGTACCTTGCAGTG‑GTGGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTg < 2:158605/246‑1 (MQ=255) gCGGGAAAAACTGCGTTACCTTGCAGAGTGTGG‑CGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTTCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTg < 1:387190/249‑1 (MQ=255) cGGGCAAAACGGCTTTACCTTGCAGTGTGTGG‑CCTTCATTCCGTGCTGTGCGTGAGTTGCGACAGGCGTTTCGCGGCGTTAACCGCGCTAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTTCCGTAGGTGATGATTTCTGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTAGTTCAGAAAGCTGGCATGTAATACGTCTCCGATATCACTAGTACGCAATTTTTTTGCGa < 2:539606/251‑1 (MQ=255) cGGGAAAAACTGCGTTACCTTGCAGAGTGTGG‑CGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCg > 1:145571/1‑250 (MQ=255) gggAAAAACTGCGTTACCTTGCAGAGTGTGG‑CGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGa > 2:399019/1‑250 (MQ=255) aaaaCTGCGTTACCTTGCAGAGTGTGT‑CGGTGATTCTGTGCTTTGCGTGCGTTTCGTCAGGAGGTTCGTGGCGTTAAACGCGAAAAATGTCATCAGCGATTGTCTGGTGAAGCTTTTGCGCGTAAAATTTCTGGCCGTAGGTGATGATTTCCGCTTTGGAGCTGGGCGTGAAGGCGATTGCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATTACCAGTACGCAACCTTTTTGTGAAgggg < 2:25160/251‑3 (MQ=255) gCGTTACCGTGCAGAGTGTGG‑CGTTGATTACGTGCTGTGCGTGCGTTGCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCAGCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTGCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGt < 2:570140/249‑1 (MQ=255) cGTTACCTTGCAAAGTGTGG‑CGTTGGTTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAAACGCGCAACATTTCATCAGCGGTCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGCGATGATTTCCGCTTTGGCGCTGGTAGTGAAGGCGGTTTCTTGCTATTACAGAAAGCTGGCATGGAATACGGATTCGTTATCCCCAGTTCGCAAACTTTTTGCgctggaggtgtgcg > 2:409839/1‑238 (MQ=255) tttACATTGCAGAGTGTGA‑CGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTATGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGTAGGCGTTTTCTTGTTATTACAGAAAGCTGGCGTGGAATACGGCGTCGATATCACCTGAACGCAAACTTTTTGAGAAggggacgaacgg > 2:91699/2‑241 (MQ=255) ccTTGCAGAGTGTGG‑CGTTGATTAAGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCCTGGAATACGGCTTCGATATTCCCAGTACGCAAACTTTTTGCGAAGGTGGCGTgggtacc > 2:256701/1‑244 (MQ=255) gCAGAGTGTGG‑CGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAACGCTGGCATGGAGTACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGAGCGTATcagc > 2:235772/1‑249 (MQ=255) ggtgtGG‑CGTTGATTACGTGCTGGGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCAcc < 2:142951/250‑1 (MQ=255) cGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGa < 2:205386/167‑1 (MQ=255) cGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGa > 1:205386/1‑167 (MQ=255) aTTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCG‑CTGTGCGTCAg < 1:533149/251‑1 (MQ=255) ttACGTGCTGTGCGTGCGTTTCGACAGGCGATTAGCGGCGTTAACCGCGCAAACTTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTCGGTGATGATTTCCGCTTTGGCGCTGGTCGCGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAACCTTTTTGCGAAGGTGGCGTGCGTATCAGCAG‑ACCGGCCATGCGTCAgg > 2:278723/1‑251 (MQ=255) tgtgCGTGCGTTTCGACAGGCATTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGGGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGGGCTGATTTCCGCTTTGGCGCTGGTCGTGTAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCAATATAACCATAACGCAAACTTTTAGCGAAGGTGGCGAGGGTATCTGCAGCAACG‑CTGTGCGTCAGGCCCtgg > 2:21186/1‑247 (MQ=255) cgggcgtTTCGACAGGCGTTACGGGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGGAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCG‑CTGTGCGTCAGGCGCTGGCGGATg < 2:547024/248‑1 (MQ=255) gtgcgtTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCG‑CTGTGCGTCAGGCGCTGGCGGATGa < 1:399019/251‑1 (MQ=255) tgcgtTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGCTCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATACGCAGCACCG‑CTGTGCGTCAGGcgcgggcggctgc > 2:336632/1‑238 (MQ=255) ttctaCAGGCGTTTCGCGGCGTTATCCGCGCAAAATTTCATCAGCGAGCTTCTGGTGAAGCTTTTGCGCGTAAAATTGCTTGCCGTAGGTGATGATTTCCTCTTTGGCGCTTGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCT‑CTGTGCGTCAGGCGCTGGCGGATGACA‑Atc < 1:121164/246‑1 (MQ=255) ttcgcaaGGCGTGTCGCGGCGTTAATCGCGCAAAATTTCATCAGCGATTTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCG‑CTGTGCGTCAGGCGCTGGCGGATGACA‑Atc < 2:798/244‑1 (MQ=255) cGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCATTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGCATCAGCAGCACCG‑CCGTGCGTCAGGCCCTTGCGGATGACA‑Atctgg > 1:583452/1‑251 (MQ=255) aGGCGTTTCGCGGCGTTAACCGCGCAAACTTTCATCAGCGATCTTCTGGTGCAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACCGCAAGCTGGCATGGAATACGGCTTCGATATCACCAGTAc > 2:29457/1‑173 (MQ=255) aGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTAc < 1:29457/173‑1 (MQ=255) aGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCG‑CTGTGCGTCAGGCGCTGGCGGATGACA‑Atctggctct < 1:133264/251‑1 (MQ=255) gcggcgTTAACCGCGCAAAATGTCAGCAGCGATCTTCTGGTGAAGCTTTTGCGTTTAAAAGTTCTTGACGTAGGTGATGATGTTCGCTTTGGCGCTGGTCGTGAAGGCGAGTTCTTGTTATTACAGAAAGCTGGCATGGAATACTGCTTCGATATCACCAGTACGCAAACTTTTGGCTAAGGTGGCGGGCGTAGCAGCAGCACCG‑CTGTGCGTCAGGCTCTTGGGGATGACA‑AGCTGGCTCTGGCagaaag < 2:677287/251‑4 (MQ=255) gTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCG‑CTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTAc < 1:248967/251‑1 (MQ=255) cgcAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCG‑CTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCAc > 1:620492/1‑251 (MQ=255) aaaaTTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCG‑CTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCg < 1:493111/251‑1 (MQ=255) tcatcaGCGATCTTCTGGTGCCGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGACGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGCAGGTGGCGTGCGTATCAGCAGCACCG‑CTGTGCGTCAGGCGCTGGCGGATG‑CACATCTGGCTCCGGCAGAAAG‑GTTACTGGGGCACCCGTTTGcc > 2:669245/1‑251 (MQ=255) caGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATcagcag < 1:19219/174‑1 (MQ=255) caGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATcagcag > 2:19219/1‑174 (MQ=255) aGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCG‑CTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCAtctc < 1:456149/251‑1 (MQ=255) aGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAAATTCTTGCCGTAGGTGATGCTTTCAGATTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACAAGTACGCAAACTTTTTGCGAAGGTGGCGAGCGTATCAGCAGCACCG‑CTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAGGTTTA‑TGGGGCACCCGTTTGCCAtctt > 2:564078/1‑250 (MQ=255) gCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCG‑CTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGACAG‑TTTACTGGGGCACCCGTTTGCAATCTcc > 2:368676/1‑251 (MQ=255) ttgTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGTTGATGATTTCCGCTTTGGCGCTTGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGTAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGTCGTGCTTATCAGCATCACCG‑CTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTAc < 1:381067/217‑1 (MQ=255) ggTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCG‑CTGTGCGTCAGGCGCTGGCGGATGACA‑ATCTGGCTCTGGCAGAAAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCgtg < 1:459223/248‑1 (MQ=255) | CTGTTACAGGGCTTGCAGGAAGAAGGGCGCAAGCGCAACTTACCGGTGATGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCAAGACTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGG‑CGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCATTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGCATCAGCAGCACCG‑CCGTGCGTCAGGCCCTTGCGGATGACA‑ATCTGGCTCTGGCAGAGAG‑TTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTG > NC_000913/21509‑21989 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |