Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	20,600	G→A	intergenic (‑92/+215)	insA1 ← / ← rpsT	IS1 family protein InsA/30S ribosomal subunit protein S20

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	20,600	0	G	A	90.0%	19.8 / ‑3.4	10	intergenic (‑92/+215)	insA1/rpsT	IS1 family protein InsA/30S ribosomal subunit protein S20
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (7/2);  total (8/2)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01

AATCAGTAAGTTGGCAGCATCACCTACCTCAATGTGTATCACAATATCCATATTCTTTGTGGGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAA‑CCCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGATTAAGCCAGTTTGTTGATCTGTGCAGTC  >  NC_000913/20540‑20842                                                             |                                                                                                                                                                                                                                                    aaaatagagtggtcttaagtttaatacctcaatgtgtatcacaatatccacatTCTTTGTAGGGG‑GTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTgcaagaacctgcaacttttgactatctggcaaccggcagtgtgttctctcacgcatcacaaaagcagcaggcataaaaaaacccgctt                                                      >  1:664311‑M1/25‑163 (MQ=255)      agagtggtcttaagtttaatacctcaatgtgtatcacaatatcCACATTCTTTGTAGGGG‑GTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTgcaagaacctgcaacttttgactatctggcaaccggcagtgtgttctctcacgcatcacaaaagcagcaggcataaaaaaacccgcttgcgcg                                                 >  1:271648‑M1/20‑158 (MQ=255)                  agtttaatacctcaatgtgTATCACAATATCCACATTCTTTGTAGGGG‑GTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTgcaagaacctgcaacttttgactatctggcaaccggcagtgtgttctctcacgcatcacaaaagcagcaggcataaaaaaacccgcttgcgcgggctttttcac                                      >  1:415251‑M1/8‑146 (MQ=255)                              cAATGTGTATCACAATATCCACATTCTTTGTAGGGG‑GTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTgcaagaacctgcaacttttgactatctggcaaccggcagtgtgttctctcacgcatcacaaaagcagcaggcataaaaaaacccgcttgcgcgggctttttcacaaagcttcagca                          >  1:574294‑M1/1‑134 (MQ=255)                               aaTGTGTATCACAATATCCACATTCTTTGTAGGGG‑GTCTGGAGATTGGGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCAACGAAGAGTTCAGGTAGTACAACGGCATGTCTgcaagaacctgacacttttgactatctggcaaccggccgtgtgttctctcacgcctcacaaaagcagcaagcataaaaaaacccgcctgtgcgggctttttaacaaaacttcagcaa                         >  1:622012‑M1/1‑133 (MQ=255)                                  gtttatcACAAGTTCCACATTCTTTGTAGGGG‑GTCTGGAGTTTGAGTAGATATGCTTGGTCAGAATTTATCAGTCGATGGTTCTACGATTCTTAAGCCACGAAGAGTGCAGATAGTACAACGGCATGTCTgcaagaacctgcaacttttgactagctggcaaccggcagtgtgtgttctcacgcatcacaaaagcagcatgcataataaaacccgcttgcgcgggctttttcacaaagcttcagcaaatgg                     <  2:395898‑M1/248‑122 (MQ=255)                                       tCACAATATCCACATTCTTTGTAGGGG‑GTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTgcaagaacctgcaacttttgactatctggcaaccggcagtgtgttctctcacgcatcacaaaagcagcaggcataaaaaaacccgcttgcgcgggctttttcacaaagcttcagcaaattggcgat                >  1:425040‑M1/1‑125 (MQ=255)                                             tatCCACATTCTTTGTAGGGG‑GTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTgcaagaacctgcaacttttgactatctggcaaccggcagtgtgttctctcacgcatcacaaaagcagcaggcataaaaa                                                               >  1:525547‑M1/1‑119 (MQ=255)                                             tatCCACATTCTTTGTAGGGG‑GTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTgcaagaacctgcaacttttgactatctggcaaccggcagtgtgttctctcacgcatcacaaaagcagcaggcataaaaa                                                               <  2:525547‑M1/198‑80 (MQ=255)                                                       cTTTGTGGGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGATTAAGCCAGTTTGTTGATCTGTGCAGTc  >  1:228912/1‑250 (MQ=255)                                                             |                                                                                                                                                                                                                                                    AATCAGTAAGTTGGCAGCATCACCTACCTCAATGTGTATCACAATATCCATATTCTTTGTGGGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAA‑CCCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGATTAAGCCAGTTTGTTGATCTGTGCAGTC  >  NC_000913/20540‑20842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑