Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	20,590	T→C	intergenic (‑82/+225)	insA1 ← / ← rpsT	IS1 family protein InsA/30S ribosomal subunit protein S20

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	20,590	0	T	C	100.0%	18.7 / NA	8	intergenic (‑82/+225)	insA1/rpsT	IS1 family protein InsA/30S ribosomal subunit protein S20
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/2);  total (6/2)

GATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTACCTCAATGTGTATCACAATATCCATATTCTTTGTGGGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAACCCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGATTAAGCCAGTTTGTT  >  NC_000913/20341‑20829                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                gATTTTCTGGTGCGTACCGGGTCGAGAAGCGGTGTAAGTGAACTACAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTACCTCAATGTGTATCACAATATCCata                                                                                                                                                                                                                                                <  1:325146/251‑1 (MQ=255)                                                                                                                                                                                                        aaaatagagtggtcttaagtttaatacctcaatgtgtatcacaatatccacatTCTTTGTAGGGG‑GTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTgcaagaacctgcaacttttgactatctggcaaccggcagtgtgttctctcacgcatcacaaaagcagcaggcataaaaaaacccgctt                                        >  1:664311‑M1/25‑163 (MQ=255)                                                                                                                                                                                                             agagtggtcttaagtttaatacctcaatgtgtatcacaatatcCACATTCTTTGTAGGGG‑GTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTgcaagaacctgcaacttttgactatctggcaaccggcagtgtgttctctcacgcatcacaaaagcagcaggcataaaaaaacccgcttgcgcg                                   >  1:271648‑M1/20‑158 (MQ=255)                                                                                                                                                                                                                         agtttaatacctcaatgtgTATCACAATATCCACATTCTTTGTAGGGG‑GTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTgcaagaacctgcaacttttgactatctggcaaccggcagtgtgttctctcacgcatcacaaaagcagcaggcataaaaaaacccgcttgcgcgggctttttcac                        >  1:415251‑M1/8‑146 (MQ=255)                                                                                                                                                                                                                                     cAATGTGTATCACAATATCCACATTCTTTGTAGGGG‑GTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTgcaagaacctgcaacttttgactatctggcaaccggcagtgtgttctctcacgcatcacaaaagcagcaggcataaaaaaacccgcttgcgcgggctttttcacaaagcttcagca            >  1:574294‑M1/1‑134 (MQ=255)                                                                                                                                                                                                                                      aaTGTGTATCACAATATCCACATTCTTTGTAGGGG‑GTCTGGAGATTGGGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCAACGAAGAGTTCAGGTAGTACAACGGCATGTCTgcaagaacctgacacttttgactatctggcaaccggccgtgtgttctctcacgcctcacaaaagcagcaagcataaaaaaacccgcctgtgcgggctttttaacaaaacttcagcaa           >  1:622012‑M1/1‑133 (MQ=255)                                                                                                                                                                                                                                         gtttatcACAAGTTCCACATTCTTTGTAGGGG‑GTCTGGAGTTTGAGTAGATATGCTTGGTCAGAATTTATCAGTCGATGGTTCTACGATTCTTAAGCCACGAAGAGTGCAGATAGTACAACGGCATGTCTgcaagaacctgcaacttttgactagctggcaaccggcagtgtgtgttctcacgcatcacaaaagcagcatgcataataaaacccgcttgcgcgggctttttcacaaagcttcagcaaatgg       <  2:395898‑M1/248‑122 (MQ=255)                                                                                                                                                                                                                                              tCACAATATCCACATTCTTTGTAGGGG‑GTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTgcaagaacctgcaacttttgactatctggcaaccggcagtgtgttctctcacgcatcacaaaagcagcaggcataaaaaaacccgcttgcgcgggctttttcacaaagcttcagcaaattggcgat  >  1:425040‑M1/1‑125 (MQ=255)                                                                                                                                                                                                                                                    tatCCACATTCTTTGTAGGGG‑GTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTgcaagaacctgcaacttttgactatctggcaaccggcagtgtgttctctcacgcatcacaaaagcagcaggcataaaaa                                                 >  1:525547‑M1/1‑119 (MQ=255)                                                                                                                                                                                                                                                    tatCCACATTCTTTGTAGGGG‑GTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTgcaagaacctgcaacttttgactatctggcaaccggcagtgtgttctctcacgcatcacaaaagcagcaggcataaaaa                                                 <  2:525547‑M1/198‑80 (MQ=255)                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                GATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTACCTCAATGTGTATCACAATATCCATATTCTTTGTGGGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAACCCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGATTAAGCCAGTTTGTT  >  NC_000913/20341‑20829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑