Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 18,169 T→C T227T (ACT→ACC)  nhaA → Na(+):H(+) antiporter NhaA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091318,1690TC94.7% 158.7 / ‑0.2 58T227T (ACT→ACC) nhaANa(+):H(+) antiporter NhaA
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base C (24/30);  total (26/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.57e-01

GTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGCACGCCGCACGGGCGTCTATATTCTTGTTGGCGTGGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACTCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTGCACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGCGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTCTGTTCT  >  NC_000913/17925‑18410
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                 
gTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCg                                                                                                                                                                                                                                             <  2:297171/251‑1 (MQ=255)
     gttccgtttgacgcgAAGATCGTGTTGATGGCTCTGGCTATTATCGTGGATCTTTGGGCCAGTATTTTCATCTCATTGTGCTACACTAATGTCTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGTTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTCGAAATCGGGGGTGCACGCAACCCTGGCgggggg                                                                                                                                                                                                                                        <  2:26699/237‑2 (MQ=255)
     gtTCCGTTAGCTCTGAAGATCTTTTGTATGGCTCTGGCTATGACCGACGATTTTGGGGCCATCATTATCATCGCATTGTTCTACTCTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATAGTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAAGCGGGGGTTCACGCAACCCTGGCggggg                                                                                                                                                                                                                                         <  2:461422/250‑1 (MQ=255)
             aGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCg                                                                                                                                                                                                                                <  1:235112/251‑1 (MQ=255)
             aGCGCTGAAGATCTGTTTGAAGGCTTTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACATTAATGACTTATCTATTGCCTCGTTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAAGCTGTTTTGTGTACGCCGCACGGGCGGCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGGTGAAATCGGGGGTTCACTCAACCCTGGCGGTGGTAATTGTc                                                                                                                                                                                                                                 <  2:73513/250‑1 (MQ=255)
               cgcTGAAGATCTTTTTGATGGCTCTGGCTATTATCGCCGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGc                                                                                                                                                                                                                              <  2:199864/251‑1 (MQ=255)
                ccTGAAGATCTTTTTTATGGCTCTGGCTTTTATCGACTATCTTGGGGCCCTCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTTCGGTTCTCGCGGTATTGAATCTGTTTGGTGTACGTCGCACGGTCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCATCCCTGGCGGGGGTAATTGTCGGct                                                                                                                                                                                                                             <  1:656856/250‑1 (MQ=255)
                   gAAGATCTTTTGTATGGCTCTGGCTATTAGCGACGATCTTTGGGCCATTATTATCATCGCATTGTTCTACACTAATGACTTATCGAGGGCCTCTCTTGGCGTGGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGGGGTGTACGCCGAGCGGGCGTCTAGATTCTGGTTGGCGGAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAAGTGTCGGcttct                                                                                                                                                                                                                          <  2:597271/251‑1 (MQ=255)
                    aaGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTTGCGGTGGTAATTGTCGGcttctt                                                                                                                                                                                                                         <  2:427146/251‑1 (MQ=255)
                     aGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTCCACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTGGGCtttttt                                                                                                                                                                                                                        >  2:488408/1‑251 (MQ=255)
                           ttttGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAAGCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTAtt                                                                                                                                                                                                                     <  1:593622/248‑1 (MQ=255)
                                  ggCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGCACGCCGCACGGGCGTCTATATTCTTGTTGGCGTGGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACTCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGaaa                                                                                                                                                                                                            <  1:292247/250‑1 (MQ=255)
                                        gtctcttatCGACGATCGTTGGGCCATCATTATCAGCGCATTGTTCTACACTAATTACTTAGCGATTGCCTCTCTTGGCGGCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGGGGTGTACGCCGCACTGGCGTCTATATTCTGGTTGGCGTAGTGGTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGaa                                                                                                                                                                                                       <  2:626040/244‑1 (MQ=255)
                                             ttatCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTTTCGGCTTCTTTTTTCCTTTGCAAGAGAAgactggg                                                                                                                                                                                                >  2:138594/1‑245 (MQ=255)
                                                            gggCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGGAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCagcga                                                                                                                                                                                   >  1:285325/1‑249 (MQ=255)
                                                               ccATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTCCTCGCGGTATTGCATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGGTCTCCagcgaagcg                                                                                                                                                                               >  1:49909/1‑250 (MQ=255)
                                                                 atcatTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACt                                                                                                                                                                            <  1:451853/251‑1 (MQ=255)
                                                                     ttATCATCGAATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTAGCGGCTGTAGCAATTGCGGTACTCGCGGTTTTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGAATGCGGTGTTGAAATCGGGGGTTCACGCAACACTGGCGGGGGTAATTTTCGGCTTCTTTATTCCTTTGAAAGGGATGCATgggggtactgcagggacacgaattgcg                                                                                                                                                                        >  2:345190/1‑227 (MQ=255)
                                                                             gCATTTTTCTACACTAATGATGTATCGATGGCATATCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGtt                                                                                                                                                                <  2:258334/251‑1 (MQ=255)
                                                                             gCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGCAGCGACTGGAGCATGTGtt                                                                                                                                                                >  1:160300/1‑251 (MQ=255)
                                                                              cagtGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGtta                                                                                                                                                               <  1:225756/248‑2 (MQ=255)
                                                                               aTTGTTCTACACTACTGACTTATCGAAGGCCTCTCTTGGCGTAGCGGCTGTAGCAAGTGCGGTACTCGCGGGATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGCGGTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTATAGGCGTCTTTATTCCTTTGAAAGAGGAGCAAGGGCGTTCTCCagcgaagcggatggaagaagtgtaac                                                                                                                                                              >  2:432280/1‑234 (MQ=255)
                                                                                    tCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCg                                                                                                                                                         >  1:547244/1‑251 (MQ=255)
                                                                                            aaTGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCgtgggtggg                                                                                                                                                 >  1:190933/1‑250 (MQ=255)
                                                                                              tGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGAGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTCTTCCTTTGAAAAAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGt                                                                                                                                               >  1:293561/1‑251 (MQ=255)
                                                                                                      cGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCCCGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGTGTTCACGCAACCCTGGCGGGGGGAATTGTCGGCTTCTTTATTTCTTTGAAAGAGAAGCCTGGGCGGTCTCCCGCGCAGCGACTGGAGCATGTGTTACACCCGAGGGTGGCGGATCTggtt                                                                                                                                       >  2:176916/1‑248 (MQ=255)
                                                                                                                           cgGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCt                                                                                                                  >  1:629589/1‑251 (MQ=255)
                                                                                                                                  aGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCa                                                                                                                                                                                       <  2:658573/175‑1 (MQ=255)
                                                                                                                                  aGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCa                                                                                                                                                                                       >  1:658573/1‑175 (MQ=255)
                                                                                                                                          cGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTc                                                                                                    >  1:212514/1‑250 (MQ=255)
                                                                                                                                                     gTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGGGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTGTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAggtgt                                                                                        <  1:97844/251‑4 (MQ=255)
                                                                                                                                                                  gtggtgTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATgg                                                                            <  1:80326/250‑1 (MQ=255)
                                                                                                                                                                       gtaCGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGCTGGCTTGAc                                                                      >  1:93678/3‑251 (MQ=255)
                                                                                                                                                                                aCGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCt                                                             >  1:429424/1‑251 (MQ=255)
                                                                                                                                                                                 cGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTTCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCCAGGTGTCAAGCTGGATGGCTTGACCTCCATTCTg                                                            >  2:198592/1‑251 (MQ=255)
                                                                                                                                                                                   ggCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGTTTTTGCCGCTGTTTCCATTTGCTACTGCTGCCGTTTCACTGCAAGGTTTCCCGCTGGATGGCTTAACCTCCATTCtccc                                                          >  1:125051/1‑248 (MQ=255)
                                                                                                                                                                                   ggCGTCTATATTCTGGTTGGCGTAGGGTTGTGGACTTCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGcc                                                          <  1:421238/251‑1 (MQ=255)
                                                                                                                                                                                     cGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGGAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGAATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCGGTTTGCATTTTCTAAGGCTGGCGTTTCACTGCAAGGGGTCACGGTGGATGGCTTGACCTCCATTCTGCCAt                                                        >  2:269988/1‑251 (MQ=255)
                                                                                                                                                                                          atatTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTgggg                                                   >  1:652491/1‑251 (MQ=255)
                                                                                                                                                                                           tattcgggttgGGGTGGTGGTGTGGTCTGCGGTGTTGAAATCGGGGGTGCACGCAACCCTGGCGGGGGTAATTGGCTGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCGGGATGGCTTGACCTCCATTCTGCCATTgggg                                                   <  2:333330/243‑1 (MQ=255)
                                                                                                                                                                                           gagtctgggtgGCGTAGTGTTGTGGACTGCGGTGTTTAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGATGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCGGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTgggg                                                   <  2:49909/241‑1 (MQ=255)
                                                                                                                                                                                             ttCTGGTTGGCGGTGTGTTGGGGACTGCGGTGTTGAAACCGGGGGTTCACGCAACCCTGGCGGGGTTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCGCCAGCGAAGCGACTGTAGCATGTGTTACTCCCGTTGGTGGCGTATCTGATTTTGCCGCTGTTTGCAGTTGCTAATGCTGTCGTTTCTCTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGat                                                 <  1:665657/250‑1 (MQ=255)
                                                                                                                                                                                                 gatgggcttatggtggtgGGCTGCGTTGTTGAAATCGGGGGTTTACGCAACCCTGGCGGTGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCg                                            <  2:201437/236‑1 (MQ=255)
                                                                                                                                                                                                        gtagtgtTGTGGACTGCGGTGTTGAAATAGGGGGTTCACGCAAACCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCtt                                     >  1:156159/4‑251 (MQ=255)
                                                                                                                                                                                                        gtaggtgtgcggACTGTGGTGTTGAAATCGGGGGTTCCCGCAACCCTGGCGGGGGTTATTTTCGGGTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGGCTGGAGCATGTGTTACTCCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGGTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCtt                                     <  2:135105/241‑1 (MQ=255)
                                                                                                                                                                                                                gtgGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGAtt                              <  1:28521/250‑1 (MQ=255)
                                                                                                                                                                                                                 tgGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTgg                            >  1:619220/1‑251 (MQ=255)
                                                                                                                                                                                                                    aCTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCaa                         <  1:432280/251‑1 (MQ=255)
                                                                                                                                                                                                                     cTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACt                                                                                                                                                                            >  2:134788/1‑103 (MQ=255)
                                                                                                                                                                                                                     cTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACt                                                                                                                                                                            <  1:134788/103‑1 (MQ=255)
                                                                                                                                                                                                                       gCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAAcc                      <  1:269988/251‑1 (MQ=255)
                                                                                                                                                                                                                       gCGGTGTTGAAAGCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAAcc                      <  1:625259/251‑1 (MQ=255)
                                                                                                                                                                                                                              tGAAATCGGGGGTTCACGCAACTCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTGCACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGCGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTgggg                                                   <  2:20084/215‑1 (MQ=255)
                                                                                                                                                                                                                              tGAAATCGGGGGTTCACGCAACTCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTGCACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGCGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTgggg                                                   >  1:20084/1‑215 (MQ=255)
                                                                                                                                                                                                                                aaaTCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGAt             >  1:439776/1‑251 (MQ=255)
                                                                                                                                                                                                                                            cACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTTAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTCTGTTct  <  2:212514/250‑1 (MQ=255)
                                                                                                                                                                                                                                                cAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGa                                                                                                                                         <  2:174956/111‑1 (MQ=255)
                                                                                                                                                                                                                                                cAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGa                                                                                                                                         >  1:174956/1‑111 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                 
GTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGCACGCCGCACGGGCGTCTATATTCTTGTTGGCGTGGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACTCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTGCACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGCGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTCTGTTCT  >  NC_000913/17925‑18410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑