Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	18,118	T→G	L210L (CTT→CTG)	nhaA →	Na(+):H(+) antiporter NhaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	18,118	0	T	G	97.9%	145.7 / ‑3.6	47	L210L (CTT→CTG)	nhaA	Na(+):H(+) antiporter NhaA
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (25/21);  total (25/22)
Fisher's exact test for biased strand distribution p-value = 4.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.11e-01

CCGGCGGCTACTGACATTGCTTTTGCACTTGGTGTACTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGCACGCCGCACGGGCGTCTATATTCTTGTTGGCGTGGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACTCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTGCACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGCGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGAT  >  NC_000913/17873‑18363                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      ccGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGACGCGGCTGTAGCAATTGCGGTACTCGGGGGATTGGATTTGTGTGGTGTACGCCGCCCGGGCGGCTTTTTTCTGGTTgg                                                                                                                                                                                                                                                  >  2:605916/1‑251 (MQ=255)        cTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCgtagtg                                                                                                                                                                                                                                           >  1:297171/1‑251 (MQ=255)            tGACATTGCCTATGCACTTGGCGTGCTGGCGCTGTTGGGACGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATAATTATCATCGCCTTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTAACCGCGGTATTGAATCTTTGTGGGGTACGCCGCACGGGCGTATATATTCTGGTTGGGGTTGtgttgg                                                                                                                                                                                                                                       >  2:206444/1‑250 (MQ=255)                 ttGCCTTTGCACTTGGTGTGCTGTCGCTGTGTGGAAGTCGTGTTCCTTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTTTTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACTCTAATGACTTAGCGTTGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTTCGGTACTCTCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGa                                                                                                                                                                                                                                    <  2:487534/249‑1 (MQ=255)                                    gcTGGCGCTGTTGGGAAGTCGTGTTCCGTTCGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGCTGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGGGGCTATATTCTGGTTGGCGTTGTGTTGAGGCCTGGGGTGTTGAAATCggggg                                                                                                                                                                                                               >  2:80326/2‑251 (MQ=255)                                                 ggAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCTGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAAccct                                                                                                                                                                                                  <  1:367849/251‑4 (MQ=255)                                                 ggAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTAGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCTCGGTATTGAATCTGTGTGGTGTACGCCGCACGGTCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGTTGTTGAAATCGGGGGTTCGCGCAAccct                                                                                                                                                                                                  >  1:79408/1‑248 (MQ=255)                                                     gTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCg                                                                                                                                                                                              <  2:297171/251‑1 (MQ=255)                                                          gttccgtttgacgcgAAGATCGTGTTGATGGCTCTGGCTATTATCGTGGATCTTTGGGCCAGTATTTTCATCTCATTGTGCTACACTAATGTCTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGTTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTCGAAATCGGGGGTGCACGCAACCCTGGCgggggg                                                                                                                                                                                         <  2:26699/237‑2 (MQ=255)                                                          gtTCCGTTAGCTCTGAAGATCTTTTGTATGGCTCTGGCTATGACCGACGATTTTGGGGCCATCATTATCATCGCATTGTTCTACTCTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATAGTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAAGCGGGGGTTCACGCAACCCTGGCggggg                                                                                                                                                                                          <  2:461422/250‑1 (MQ=255)                                                                  aGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCg                                                                                                                                                                                 <  1:235112/251‑1 (MQ=255)                                                                  aGCGCTGAAGATCTGTTTGAAGGCTTTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACATTAATGACTTATCTATTGCCTCGTTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAAGCTGTTTTGTGTACGCCGCACGGGCGGCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGGTGAAATCGGGGGTTCACTCAACCCTGGCGGTGGTAATTGTc                                                                                                                                                                                  <  2:73513/250‑1 (MQ=255)                                                                    cgcTGAAGATCTTTTTGATGGCTCTGGCTATTATCGCCGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGc                                                                                                                                                                               <  2:199864/251‑1 (MQ=255)                                                                     ccTGAAGATCTTTTTTATGGCTCTGGCTTTTATCGACTATCTTGGGGCCCTCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTTCGGTTCTCGCGGTATTGAATCTGTTTGGTGTACGTCGCACGGTCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCATCCCTGGCGGGGGTAATTGTCGGct                                                                                                                                                                              <  1:656856/250‑1 (MQ=255)                                                                        gAAGATCTTTTGTATGGCTCTGGCTATTAGCGACGATCTTTGGGCCATTATTATCATCGCATTGTTCTACACTAATGACTTATCGAGGGCCTCTCTTGGCGTGGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGGGGTGTACGCCGAGCGGGCGTCTAGATTCTGGTTGGCGGAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAAGTGTCGGcttct                                                                                                                                                                           <  2:597271/251‑1 (MQ=255)                                                                         aaGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTTGCGGTGGTAATTGTCGGcttctt                                                                                                                                                                          <  2:427146/251‑1 (MQ=255)                                                                          aGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTCCACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTGGGCtttttt                                                                                                                                                                         >  2:488408/1‑251 (MQ=255)                                                                                ttttGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAAGCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTAtt                                                                                                                                                                      <  1:593622/248‑1 (MQ=255)                                                                                       ggCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGCACGCCGCACGGGCGTCTATATTCTTGTTGGCGTGGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACTCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGaaa                                                                                                                                                             <  1:292247/250‑1 (MQ=255)                                                                                             gtctcttatCGACGATCGTTGGGCCATCATTATCAGCGCATTGTTCTACACTAATTACTTAGCGATTGCCTCTCTTGGCGGCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGGGGTGTACGCCGCACTGGCGTCTATATTCTGGTTGGCGTAGTGGTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGaa                                                                                                                                                        <  2:626040/244‑1 (MQ=255)                                                                                                  ttatCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTTTCGGCTTCTTTTTTCCTTTGCAAGAGAAgactggg                                                                                                                                                 >  2:138594/1‑245 (MQ=255)                                                                                                                 gggCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGGAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCagcga                                                                                                                                    >  1:285325/1‑249 (MQ=255)                                                                                                                    ccATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTCCTCGCGGTATTGCATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGGTCTCCagcgaagcg                                                                                                                                >  1:49909/1‑250 (MQ=255)                                                                                                                      atcatTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACt                                                                                                                             <  1:451853/251‑1 (MQ=255)                                                                                                                          ttATCATCGAATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTAGCGGCTGTAGCAATTGCGGTACTCGCGGTTTTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGAATGCGGTGTTGAAATCGGGGGTTCACGCAACACTGGCGGGGGTAATTTTCGGCTTCTTTATTCCTTTGAAAGGGATGCATgggggtactgcagggacacgaattgcg                                                                                                                         >  2:345190/1‑227 (MQ=255)                                                                                                                                  gCATTTTTCTACACTAATGATGTATCGATGGCATATCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGtt                                                                                                                 <  2:258334/251‑1 (MQ=255)                                                                                                                                  gCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGCAGCGACTGGAGCATGTGtt                                                                                                                 >  1:160300/1‑251 (MQ=255)                                                                                                                                   cagtGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGtta                                                                                                                <  1:225756/248‑2 (MQ=255)                                                                                                                                    aTTGTTCTACACTACTGACTTATCGAAGGCCTCTCTTGGCGTAGCGGCTGTAGCAAGTGCGGTACTCGCGGGATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGCGGTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTATAGGCGTCTTTATTCCTTTGAAAGAGGAGCAAGGGCGTTCTCCagcgaagcggatggaagaagtgtaac                                                                                                               >  2:432280/1‑234 (MQ=255)                                                                                                                                         tCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCg                                                                                                          >  1:547244/1‑251 (MQ=255)                                                                                                                                                 aaTGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCgtgggtggg                                                                                                  >  1:190933/1‑250 (MQ=255)                                                                                                                                                   tGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGAGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTCTTCCTTTGAAAAAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGt                                                                                                >  1:293561/1‑251 (MQ=255)                                                                                                                                                           cGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCCCGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGTGTTCACGCAACCCTGGCGGGGGGAATTGTCGGCTTCTTTATTTCTTTGAAAGAGAAGCCTGGGCGGTCTCCCGCGCAGCGACTGGAGCATGTGTTACACCCGAGGGTGGCGGATCTggtt                                                                                        >  2:176916/1‑248 (MQ=255)                                                                                                                                                                                cgGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCt                                                                   >  1:629589/1‑251 (MQ=255)                                                                                                                                                                                       aGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCa                                                                                                                                        <  2:658573/175‑1 (MQ=255)                                                                                                                                                                                       aGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCa                                                                                                                                        >  1:658573/1‑175 (MQ=255)                                                                                                                                                                                               cGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTc                                                     >  1:212514/1‑250 (MQ=255)                                                                                                                                                                                                          gTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGGGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTGTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAggtgt                                         <  1:97844/251‑4 (MQ=255)                                                                                                                                                                                                                       gtggtgTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATgg                             <  1:80326/250‑1 (MQ=255)                                                                                                                                                                                                                            gtaCGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGCTGGCTTGAc                       >  1:93678/3‑251 (MQ=255)                                                                                                                                                                                                                                     aCGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCt              >  1:429424/1‑251 (MQ=255)                                                                                                                                                                                                                                      cGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTTCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCCAGGTGTCAAGCTGGATGGCTTGACCTCCATTCTg             >  2:198592/1‑251 (MQ=255)                                                                                                                                                                                                                                        ggCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGTTTTTGCCGCTGTTTCCATTTGCTACTGCTGCCGTTTCACTGCAAGGTTTCCCGCTGGATGGCTTAACCTCCATTCtccc           >  1:125051/1‑248 (MQ=255)                                                                                                                                                                                                                                        ggCGTCTATATTCTGGTTGGCGTAGGGTTGTGGACTTCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGcc           <  1:421238/251‑1 (MQ=255)                                                                                                                                                                                                                                          cGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGGAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGAATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCGGTTTGCATTTTCTAAGGCTGGCGTTTCACTGCAAGGGGTCACGGTGGATGGCTTGACCTCCATTCTGCCAt         >  2:269988/1‑251 (MQ=255)                                                                                                                                                                                                                                               atatTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTgggg    >  1:652491/1‑251 (MQ=255)                                                                                                                                                                                                                                                  ttCTGGTTGGCGGTGTGTTGGGGACTGCGGTGTTGAAACCGGGGGTTCACGCAACCCTGGCGGGGTTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCGCCAGCGAAGCGACTGTAGCATGTGTTACTCCCGTTGGTGGCGTATCTGATTTTGCCGCTGTTTGCAGTTGCTAATGCTGTCGTTTCTCTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGat  <  1:665657/250‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      CCGGCGGCTACTGACATTGCTTTTGCACTTGGTGTACTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGCACGCCGCACGGGCGTCTATATTCTTGTTGGCGTGGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACTCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTGCACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGCGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGAT  >  NC_000913/17873‑18363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑