| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,860 | T→A | A508A (GCT→GCA) | thrA → | fused aspartate kinase/homoserine dehydrogenase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,860 | 0 | T | A | 97.1% | 214.7 / NA | 72 | A508A (GCT→GCA) | thrA | fused aspartate kinase/homoserine dehydrogenase 1 |
| Reads supporting (aligned to +/- strand): ref base T (1/1); major base A (27/41); minor base C (2/0); total (30/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.68e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAA‑TGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTAC > NC_000913/1615‑2103 | aTTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCt < 2:648993/251‑1 (MQ=255) gtggcgacatggcgtatggatctggtGAACGCTCAAACGCTGTCGTGGTAAATAACGATGATGCGACCACTGGTGTGCGCGTTTCTCATCAGATGCTGTTCAATCCCGATCAGGTTATCGATGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGCAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTc < 2:324984/226‑1 (MQ=255) cGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGCTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCa < 2:301528/251‑1 (MQ=255) cATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAA‑Tg > 1:58181/1‑251 (MQ=255) cataGGGATCTTCGGAAAGCTCAATCTCTGTCGGGGTAAATAACGAAGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAA‑TGTGCAt < 2:125033/248‑1 (MQ=255) gggATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGCTCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGGAGCGTCCACAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACCCGAAGGCAATACCCACCAA‑TGTGCATGGcc > 2:463057/1‑251 (MQ=255) ggATCTTCTGAAAGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGATATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAA‑TGTGCATGGCCt > 1:112668/1‑251 (MQ=255) tctGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAA‑TGTGCATGGCCTAAATc < 1:253589/250‑1 (MQ=255) gAACGCTCAATCGCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAA‑TGTGCATGGTCTAAATCTg < 1:107304/249‑1 (MQ=255) aaCGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAA‑TGTGCATGGCCTAAATCTGGa > 1:666637/1‑250 (MQ=255) aCGCTCAATCTATGTCGTGGGAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATACTGGTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGAGACGGTGGCGTTGACGGTACGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGATGAAGAATAAACATATCGCCTTAAGTGTCTGCGGTGTTGACAACTCGACGGACCTGCTCACCAA‑Tgggcagggcctacaagtggta > 2:420680/1‑230 (MQ=255) cAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAA‑TGTGCATGGCCTAAATCTGGAAAACt < 1:227325/249‑1 (MQ=255) cAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAA‑TGTGCATGGCCTAAATCTGGAAAACTgg < 1:256849/251‑1 (MQ=255) cAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAA‑TGTGCATGGCCTAAATCTGGAAAACTg < 1:51793/250‑1 (MQ=255) cAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCGGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCGACTTACGTTTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAA‑TGTGCATGGCCTAAATCTGGAAAACt < 2:374563/249‑1 (MQ=255) gTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAAAATAAACATATCGACTTACGTGTCTGCGGGGTTTACAACTCGAAGGCACTGCTCACACA‑TGTGCATGGCCTAAATCTGGAAAACTGCCAGga > 2:576554/1‑248 (MQ=255) ggTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCTACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAA‑TGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTgg < 1:626377/251‑1 (MQ=255) aTAACGATGAAGCGACCACTGGCATGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGGGGCGTTGGCGGTGCGATGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCGACTTACGTATCTGCGGTGTTGCCAACCCGAAGTACCTGCTCACCAAATGGAC‑TGGACCAAATCTGGAAAACTGGCAGgaaaaaacggcccc > 2:444113/1‑239 (MQ=255) aaCGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAA‑TGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGGACTGGCGCAAGc < 1:501096/251‑1 (MQ=255) cGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAA‑TGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCa < 1:290569/251‑1 (MQ=255) atgCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAA‑TGTGCATGGCCTAAAt < 1:3764/212‑1 (MQ=255) atgCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAA‑TGTGCATGGCCTAAAt > 2:3764/1‑212 (MQ=255) atgCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAA‑TGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAagag < 1:268555/251‑1 (MQ=255) aCCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAA‑TGTGCATTGCCTAAATCTGGAAAACTGGCCGGAAGAACTGGCGCAAGCCAAAGAGCCGtt > 2:20377/1‑251 (MQ=255) aCTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAA‑TGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTaa < 1:20377/251‑1 (MQ=255) gtcgttctcgTTACTCTGCAGATGCTGTTCAAGTCCGATCAGGTTATCGACGTGTTTGTGATTGGCGTCGGTGTCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCGACTTACTTGTCTTCGGTGTTGCCAACTCGAAGGCACTGCTCACCAA‑TGTGCATGGCCGAAATCTTGAAAACTGGCAGGAAGAACTTGCGTAAGCCAAATAGCCGTTTAAt < 2:34004/241‑1 (MQ=255) gTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAA‑TGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCgg < 1:249673/251‑1 (MQ=255) gTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTAGCGTCGGTGGCGTTGGCGGTGCGATGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACAAATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAA‑TGTGCATGGCCTAAAACTGGAAAACTGGCAGGAAGAACTGGACCAAGCCAAAGAACCGTTTAATATCGGGCGCtt > 2:477947/1‑251 (MQ=255) cTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATCTCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAA‑TGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGACAAAGAGCCGTTTAATCTAGGGCGCTTAAtt > 2:163745/1‑251 (MQ=255) catctGATGCTGGTCAATTCCGATCAGGTTATCGAAGTGTTTGGGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAATAATAAACATGTCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCGTACCAA‑TGTGCATGGCCTAAATCTGGAAAACGGGCAGGAAGAACTGGCGCAAGCTATAGAGCCGTTTAATCTCGGGCGCTTAATTc < 2:655808/250‑1 (MQ=255) catcaGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGGCGGTGGCGTTGGCGGTGCGTTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAA‑TGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTc < 1:388932/250‑1 (MQ=255) catcaGATGCTGTTCAATACCGATCAGGTTATCGAAGTGGTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAA‑TGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCg < 1:247855/251‑1 (MQ=255) catcaGAGGCTGTTCAATACCGATCAGGTTATCGAAGTGGTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAA‑TGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCg < 1:617089/251‑1 (MQ=255) aGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAA‑TGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTc < 1:609608/251‑1 (MQ=255) ggtgtggTTCAATACCGAGCAGGTTTTCGAAGTGGTTGTGATTGGCTGCGGTGGCGTGGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAA‑TGTGCATGGCCTAAATCTGGAAAACGGGCAGGAAGAACTGGCGCAAGCCAAAGAGCTGTTTAATCTCTGGCGCTTAATTCGCCTCg < 2:400836/245‑1 (MQ=255) aCCGATCAGGTTCTCGTAGTGTTTGTGATTCACGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAGAGCTGGCTGGGGAATAACCATATCGGCTTACGTGTCTGCGGTGTTGCCAACTCGCAGGCACTGCTCCCCAC‑TGAGCATGGCCTAAATCTGGaa > 2:73224/1‑174 (MQ=255) aCCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAA‑TGTGCATGGCCTAAATCTGGaa < 1:73224/174‑1 (MQ=255) aGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGAGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAA‑TGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATctgctg > 1:41477/1‑251 (MQ=255) ttATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGCATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACAAA‑TGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTAGAGCAAGCAAAAGCGCAGTTTTATATCGGGCGCTTTATTCGCCTCGTGAAAGAATATCATCTGATGAAc > 2:29525/1‑251 (MQ=255) ttATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAA‑TGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAAc > 1:439176/1‑251 (MQ=255) tATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAA‑TGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAAt > 2:125305/1‑205 (MQ=255) tATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAA‑TGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAAt < 1:125305/205‑1 (MQ=255) aTCGAAGTGTTTGGGATTGGCGGCGGGGGAGTAGGCGGTGCGCTTCTGGTGCAACTGAATCGTCAACAAAGCGTGCTGATGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCATTGCTCACCAA‑TGTGCATGGCCTAAATTTGGAAAACTGGCAGGAAGAACTGGCGCAAGACAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAAcc < 2:375278/250‑1 (MQ=255) cGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAA‑TGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCg < 1:156721/250‑1 (MQ=255) tgtTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAA‑TGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCCAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGCAAGAATATCATCTGCTGAACCCGGTCAtt > 2:563405/1‑251 (MQ=255) gtTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAA‑TGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCAttg < 1:545447/251‑1 (MQ=255) gATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAA‑TGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACt > 2:176529/1‑251 (MQ=255) gATTGGAGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAA‑TGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCCCCCCGTGAAAAAATATCATCTTCTGACCCCGGTCATTGTTGACt > 2:280962/1‑251 (MQ=255) aTTGGTGTCTGTGGCGTTGGGGGTTCGTTGATGGAGCAACTGAGGCGTCAATAACGCTGGCTGAAGAAGAAACATATCGTCTGACGTGTCTGCTGTGTTGCCAACTCGAAGGCACAGCACACCAA‑TGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACGGGCGCAAGCCAAATAGCCGTTTAATCACGGGCGCGTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGGCATTGTTGACt < 2:543366/250‑1 (MQ=255) tGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGACTAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAA‑TGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGCACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGccg > 2:261741/1‑249 (MQ=255) tgGAGCAACTGAAGCGTCAACAAAGCTGGCTGCAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAA‑TGt > 2:352448/1‑97 (MQ=255) tgGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAA‑TGt < 1:352448/97‑1 (MQ=255) aGCAACTGAAGCGTCAACAAAGCTGTCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAA‑TGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGcc < 1:611862/251‑1 (MQ=255) aGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAA‑TGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGcc < 1:344392/251‑1 (MQ=255) cAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAA‑TGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATgacgg > 1:89258/1‑247 (MQ=255) tGAAGCGTCAACAAAGCTGTCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAA‑TGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGACCAATATGCCgccctc > 2:673986/1‑246 (MQ=255) aGCGTCAACAAATATGGCTGAAGAATAATCATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAA‑TGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACGGGCGCAAGCCAACGAGACGTTTAATCTCGGGCGCTTAATTAGCCTCGTGAACGAATATCATCTGCTGAACCCGGTCCTTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGGTCAATATGCCGACtttctt > 2:378470/1‑247 (MQ=255) aGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAA‑TGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTGCGCGAAGGttt < 1:373944/251‑1 (MQ=255) agaaTAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAA‑TGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATTAGGCGAATTCTTGCGCGAAGGTTTCCACGTTGTc > 1:476267/1‑251 (MQ=255) aaaCATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAA‑TGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGcc < 1:463057/251‑1 (MQ=255) aaaCATAACGACTTACGTGTCGGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAA‑TGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGcc < 2:163702/251‑1 (MQ=255) cgattgaCGTGTCGTCGGTGGTGCCAACTAGAAGGAACTTCGAACCAA‑TGTGCCTGGCCTAAATCTGGAAAACTTGCAGGAAGAACTGGCGTAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTTAGTCGCCTCGTGAAAGAAGATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCTGAGCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGGCTCGCCGAACaaa < 2:289873/244‑1 (MQ=255) gACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAA‑TGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGGTGTCACGCCGAACcaaa > 1:378867/1‑250 (MQ=255) aCTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAA‑TGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCGCTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTGCGCGAAGGGTTCCACGTTGTCACGCCGAACaaaa > 1:329781/1‑249 (MQ=255) ttACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAA‑TGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGCCTTCTTGCGCGAAGGGTTCCACGTTGTCACGCCGAACAAAAAGGc > 1:113085/1‑251 (MQ=255) aCGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAA‑TGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGcc < 1:110532/250‑1 (MQ=255) tGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAA‑TGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCAACCCGCACAAAAAAGCCAACAccac > 2:599376/1‑249 (MQ=255) gtTGCCAACTCGAAGGCTCTGCTCACCAA‑TGTACATGGCCTTAATCGGGAAAACTGGCAGGAAGAACTGGCGAAAGCCAAAGTGCCGCTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCAGCTGCCGAACCCGGTCATTGTTGACTGCACTTCCAGCCATGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGTCCAACACCTCGTCGAt < 2:41477/251‑1 (MQ=255) gtTGCCAACTCGAAGGCACTGCTCACCAA‑TGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGAt < 1:176529/251‑1 (MQ=255) cTCGAAGGCACTGCTCACCAA‑TGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTcca > 1:68169/1‑155 (MQ=255) cTCGAAGGCACTGCTCACCAA‑TGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTcca < 2:68169/155‑1 (MQ=255) cGAAGGCACTGCTCACCAA‑TGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCTCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATtactac < 1:132325/251‑1 (MQ=255) | ATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAA‑TGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTAC > NC_000913/1615‑2103 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |