Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	393	T→G	R19R (CGT→CGG)	thrA →	fused aspartate kinase/homoserine dehydrogenase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	393	0	T	G	100.0%	113.1 / NA	37	R19R (CGT→CGG)	thrA	fused aspartate kinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (22/15);  total (22/15)

AGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGA‑CAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAAC‑GTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCG‑TCCTCTCTGCCCCCGCCAAAATCAC‑CAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCA  >  NC_000913/174‑635                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                     aaagTACAAAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGCGCTGACGCGTACAGGAAACACAGAAAAAAGCACGCACATGA‑CAGTGAGGACTTATTTTT‑CGACCAAAGGGAACGAAGTAACAACCATGAGAGGGTTGAGGTTAGCAGGGACAAAAGTAGCAACTGCAGAAC‑GTTTGCTGTGGGTTGACGAAATTCTGGaaaagcattgcaggc                                                                                                                                                                                                                       >  2:433329/3‑238 (MQ=255)    gTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGA‑CAGTGCGGGCTTTTTTTT‑CGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAAC‑GTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAggcaggg                                                                                                                                                                                                                   >  1:518965/1‑251 (MQ=255)         caacaTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGA‑CAGTGCGGGCTTTTTTTT‑CGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAAC‑GTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAggcaggggcagg                                                                                                                                                                                                              >  1:255277/1‑251 (MQ=255)         caacaCCCATGAAACGCATTAGCACCACCATTACCACCACCAGCACCATTACCACAGGTAACGGGGCGTGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGA‑CAGTGCGGGCTTTTTTTT‑CGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAAC‑GTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAggcaggggcagg                                                                                                                                                                                                              <  2:518965/251‑1 (MQ=255)           acaTCCATGAAACGCATTAGCACCACCATGACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGA‑CAGAGCGGGCTTTTTTTT‑CGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGGACATCAGTGGCAAATGAAGAAC‑GTTTTATGTGGGTTGTCGCGATTCTCGAAAGCAATGcctggaagtgagagggg                                                                                                                                                                                                            >  2:471157/1‑236 (MQ=255)                       cGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGA‑CAGTGCGGGCTTTTTTTT‑CGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGAGGCAAATGCAGAAC‑GTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCG‑TCctc                                                                                                                                                                                               >  1:23601/1‑251 (MQ=255)                                       ttACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGA‑CAGTGCGGGCTTTTTTTT‑CGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAAC‑GTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCG‑TCCTCTCTGCCCCCGCCaaa                                                                                                                                                                                >  1:355054/1‑250 (MQ=255)                                                     cccactACCACAGGTAACGGTGCGGGCTGACGCTGCCGGGAAACACTAAAAAAAGCCCGCACCTGA‑CAGTGCGGGCTTTTTTTT‑CGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTGGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAAC‑GTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGCCAGGTGGC‑ACCGGCCCTCTCTGCCCCCGCCAAAATCAC‑CAACCACCtg                                                                                                                                                                <  2:122079/246‑1 (MQ=255)                                                                               cTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGA‑CAGTGCGGGCTTTTTTTT‑CGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAAC‑GTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCG‑TCCTCTCTGCCCCCGCCAAAATCcc‑ca                                                                                                                                                                        >  2:183687/1‑217 (MQ=255)                                                                               cTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGA‑CAGTGCGGGCTTTTTTTT‑CGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAAC‑GTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCG‑TCCTCTCTGCCCCCGCCAAAATCac‑ca                                                                                                                                                                        <  1:183687/217‑1 (MQ=255)                                                                                      gTACAGGAAACACAGAAAAAAGCCCGCACCTGA‑CAGTGCGGGCTTTTTTTT‑CGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAAC‑GTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCG‑TCCTCTCTGCCCCCGCCAAAATCAC‑CAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGcc                                                                                                                                 >  1:270585/1‑249 (MQ=255)                                                                                      gTACAGGAAACACAGAAAAAAGCCCGCACCTGA‑CAGTGCGGGCTTTTTTTT‑CGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAAC‑GTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCG‑TCCTCTCTGCCCCCGCCAAAATCAC‑CAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAg                                                                                                                               <  1:535731/251‑1 (MQ=255)                                                                                         cAGGAAACACAGAAAAAAGCCCGCACCTGA‑CAGTGCAGGCTTTTTTTT‑CGACCAAAGGAAACGGGGTAACAACCATGCGATTGTTGAAGTTCGGCGGTACATCCGTGGCAAATGCAGCAC‑GTTTTCTGCGGGTTGTCGATATTCTGGAAAGCAATGCCAGGCAGGGGCCGGGGGCCGCCG‑CCATCTCTCCCCCCGCCACAATCAC‑CAACCACCTGGGGGCGGTGATTGAACAAACCATTacagcccagggt                                                                                                                            >  1:327687/1‑240 (MQ=255)                                                                                                 acaGAAAAAAGCCCGCACCTGA‑CAGTGCGGGCTTTTTTTT‑CGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAAC‑GTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCG‑TCCTCTCTGCCCCCGCCAAAATCAC‑CAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTAcc                                                                                                                    <  1:638676/251‑1 (MQ=255)                                                                                                 aaaaaaaaaaACCCCCACCTTAACAGTG‑GGGGTTTTTTTTCCGACCAAAGTTAAAGCGGTAACAACCATGCGAGGGTGTAAGTTCGGCGGTACATCAGTGGCAAATGCAGACC‑GTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCG‑TCCTCTCTGCCCCCGCCAAAATCAC‑CAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTAc                                                                                                                     <  2:158704/247‑1 (MQ=255)                                                                                                  caaaaaaaaGCCCGCCCCGGA‑CGGTGCGGGCTTTTTTTT‑CTACCAAAGTTAGCGAGGTAACAACCGTGCGAGTGTGGAAGATGGGCGGTACATCAGTGGCAAGTGCAAACC‑GTTCTCCGCGGGTTGCCGATATCCTGGAAAGCAAGCCCAGGCAGGGGCGGGTGGCCACCG‑TCCTCGCTACCCCCGCCAAAATCAC‑CAACCACCTGGTGTCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTAccc                                                                                                                   <  2:310760/248‑1 (MQ=255)                                                                                                   aGAAAAAAGCCCGCACCTGA‑CAGTGCGGGCTTTTTTTT‑CGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAAC‑GTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCG‑TCCTCTCTGCCCCCGCCAAAATCAC‑CAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTAcccc                                                                                                                  >  1:463083/1‑250 (MQ=255)                                                                                                   aGAAAAAAGCCCGCACCTGA‑CAGTGCGGGCTTTTTTTT‑CGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAAC‑GTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCG‑TCCTCTCTGCCCCCGCCAAAATCAC‑CAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCa                                                                                                                  <  1:503385/251‑1 (MQ=255)                                                                                                              cGCAACTGA‑CAGTGCGGGCTTTTTTTT‑CGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGAGGCAAATGCAGAAC‑GTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCCGGTGGCCACCG‑TCCTCTCTGCCCCCGCCAAAAT‑ACACAACCACCTGGTGGCGATGCTTGAAAAAACCATTAACGGCCCGGATACTTTACCCCATATCAGCGAt                                                                                                       >  2:587779/1‑251 (MQ=255)                                                                                                                           tGCGGGCTTTTTTTT‑CGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAAC‑GTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCG‑TCCTCTCTGCCCCCGCCAAAATCAC‑CAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTAtt                                                                                           >  1:12157/1‑251 (MQ=255)                                                                                                                                                aaaGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAAC‑GTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCG‑TCCTCTCTGCCCCCGCCAAAATCAC‑CAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACggg                                                                       >  1:488129/1‑251 (MQ=255)                                                                                                                                                          gtgTAACACCCATGCGAGTGTGTAAGTTCGGCGGTATGTCAGTGACAAATGC‑GAACCGTTTTCTGCGGTTTGCCGATATGCTGGAAAGCAATGCCAGGCAGGGGAAGGTGGCCACCG‑TCCACTCTGCCCCCGCCAAAATCAC‑CAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGACTcgccgcc                                                             <  2:255277/249‑1 (MQ=255)                                                                                                                                                                   ccaggCGAGTGTTGAAGTTCGGCGGTACACCAGTGGCAAATGCAGAAC‑GTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCT‑TCCTCTCTGCCCCCGCCAAAATCAC‑CAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCttt                                                                                                                       <  1:656181/180‑1 (MQ=255)                                                                                                                                                                    cATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAAC‑GTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCG‑TCCTCTCTGCCCCCGCCAAAATCAC‑CAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCgg                                                   >  1:175105/1‑251 (MQ=255)                                                                                                                                                                        cGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAAC‑GTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCG‑TCCTCTCTGCCCCCGCCAAAATCAC‑CAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACgg                                                                        <  1:191701/226‑1 (MQ=255)                                                                                                                                                                         gAGTGTTGAAGTTCGGCGTTACACCAGTGGCAAATGCAGACC‑GTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGTTGGCCACCG‑CCCTCTCTGCCCCCGCCAAAATCAC‑CAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCtt                                                                                                                        <  2:292340/177‑1 (MQ=255)                                                                                                                                                                         gAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAAC‑GTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCG‑TCCTCTCTGCCCCCGCCAAAATCAC‑CAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCtt                                                                                                                        >  1:292340/1‑177 (MQ=255)                                                                                                                                                                          ggtgtTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAAC‑GTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCG‑TCCTCTCTGCCCACGCCAAAATCAC‑CAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCGGGATTcc                                             >  1:316648/2‑251 (MQ=255)                                                                                                                                                                             gtTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAAC‑GTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCG‑TCCTCTCTGCCCCCGCCAAAATCAC‑CAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCGGGATTCCCg                                           <  1:641201/250‑1 (MQ=255)                                                                                                                                                                             gtTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAAC‑GTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCG‑TCCTCTCTGCCCCCGCCAAAATCAC‑CAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCGGGATTCCCGc                                          >  1:390834/1‑251 (MQ=255)                                                                                                                                                                              ttGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAAC‑GTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCG‑TCCTCTCTGCCCCCGCCAAAATCAC‑CAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCGGGATTCCCGCt                                         >  1:408513/1‑251 (MQ=255)                                                                                                                                                                                                 cAGTGGCAAATGCAGAAC‑GTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCG‑TCCTCTCTGCCCCCGCCAAAATCAC‑CAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCGGGATTCCCGCTGGCGCAATTGAAAACTTtc                      >  1:211837/1‑251 (MQ=255)                                                                                                                                                                                                      gCAAATGCAGAAC‑GTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCG‑TCCTCTCTGCCCCCGCCAAAATCAC‑CAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTTACGGGACTCGCCGCCGCCCAGCCGGGTTTCCCGCTGGCGCAATTGAAAACTTtcgtcgg                 >  2:650196/1‑250 (MQ=255)                                                                                                                                                                                                           tGCAGAAC‑GTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCG‑TCCTCTCTGCCCCCGCCAAAATCAC‑CAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCGGGATTCCCGCTGGCGCAATTGAAAACTTTCGTCGCTCAgg            >  2:237918/1‑251 (MQ=255)                                                                                                                                                                                                               gAAC‑GTTTTCGGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCG‑TCCTCTCTGCCCCCGCCAAAATCAC‑CAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCGGGATTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAAtt        <  1:237918/251‑1 (MQ=255)                                                                                                                                                                                                                    gTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCG‑TCCTCTCTACCCCCGCCAAAATCAC‑AAACCACCTGGTTGCGCTGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGAATTTTTGCCGAACTTTTGTCGGGACTCGCCGCCTCCCAGCCCGGATTCCCGCTGGCGCCATTGAAAACTTTCGTCGTTCAGGACTTTGcc    >  1:113422/1‑251 (MQ=255)                                                                                                                                                                                                                       ttCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCG‑TCCTCTCTGCCCCCGCCAAAATCAC‑CAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCGGGATTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCa  <  1:130185/250‑1 (MQ=255)                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                     AGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGA‑CAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAAC‑GTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCG‑TCCTCTCTGCCCCCGCCAAAATCAC‑CAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCA  >  NC_000913/174‑635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑