Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,593	A→G	A419A (GCA→GCG)	thrA →	fused aspartate kinase/homoserine dehydrogenase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,593	0	A	G	97.2%	195.3 / NA	75	A419A (GCA→GCG)	thrA	fused aspartate kinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base A (1/1);  major base G (32/38);  minor base T (1/1);  total (35/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCACTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCT  >  NC_000913/1356‑1837                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                         aCGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGGGATG‑GTATGCGGAACTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGccc                                                                                                                                                                                                                                             >  1:10537/1‑250 (MQ=255)         gTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAAc                                                                                                                                                                                                                                    <  1:150850/251‑1 (MQ=255)             ttCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATG‑GGATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTg                                                                                                                                                                                                                                >  1:93488/1‑251 (MQ=255)               ccGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGGGATGTGT‑TGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCACCATTGTc                                                                                                                                                                                                                              >  2:110947/1‑251 (MQ=255)                 gtggtgCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATG‑GTATGCGCAACTTGCGTGGGGTCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTTTC‑Gc                                                                                                                                                                                                                           >  1:655808/1‑251 (MQ=255)                             aCGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAgg‑g                                                                                                                                                                                                              <  1:8296/251‑1 (MQ=255)                              cGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAgg‑g                                                                                                                                                                                                              >  1:288402/1‑250 (MQ=255)                              cGCAATCATCTGCCGAATACGGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑Ga                                                                                                                                                                                                             <  2:340465/251‑1 (MQ=255)                                cAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGGAGGGGATG‑GTCAGGGCACCCTTCGGGGGATCTCGGCGAAAATCTTTGCCGCCCTGGCCCCCCCCA‑AGAACCACATTGTCCGCC‑TTGCTCAgg‑ggt                                                                                                                                                                                                            >  2:290569/1‑248 (MQ=255)                                     atcTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGGGATG‑GGATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCCTTGCTCAGG‑GATCTTCTg                                                                                                                                                                                                      >  1:400836/1‑251 (MQ=255)                                        ctCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCCACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAAc                                                                                                                                                                                                   >  1:193722/2‑251 (MQ=255)                                         tCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGa                                                                                                                                                                                                     <  1:352537/248‑1 (MQ=255)                                          ccGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACg                                                                                                                                                                                                  <  1:274611/250‑1 (MQ=255)                                          cagaatgcttgatctgTTTCTGCGTTCCGCAAAGCGACTGTGTGCGTGCTGAACGGGCACTGCAGTAAGTGTTCTTCCTGTATCTGACAGAATGCTTCCTGGTGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATG‑GTATGCGCACCGTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATACCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGc                                                                                                                                                                                                 <  2:514732/236‑1 (MQ=255)                                             aataaagtaTCAGGTTCTGCGTTCGGCAAAGCGACGGTGTGCGAGCGGAACGGGCAATGCAGGAAGAGTTCTACCAGGAACTGAAAGAAGGCTTGCTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCa                                                                                                                                                                                              <  2:251315/243‑1 (MQ=255)                                             aaTACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGATCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGGAGGGGAGG‑GGAAGAGAACCCTGAGTGGGCTATCGGCGAAAATCTTTGCCGCGCTGGCCCGCGACA‑AAATAAACAATGTC‑GCACTTGCTCAGGAGA‑CTTCTGAAcccacc                                                                                                                                                                                              >  2:439439/1‑246 (MQ=255)                                                tcagtaTCAGATTCTGCGTTCCGCAAAGCGACTGTGTGAGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACAGCTGGCAATTATCTCGGTGGTAGGCGGTA‑GTAAGCGCACCTTGCGTGGGATATCGAGGAAATTCTTTGCAGGTCTGGCCCGC‑CAACATTTCAACCTTTTC‑GCCCTAGCACAGG‑GATCTTCTTAACGcccaca                                                                                                                                                                                            >  2:510935/6‑245 (MQ=255)                                                aCAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAAtc                                                                                                                                                                                           <  1:237356/251‑1 (MQ=255)                                                 cagtatccgTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GGTCTTCTGAACGCTCAAtct                                                                                                                                                                                          >  1:240711/9‑251 (MQ=255)                                                  agtaTCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGGGGGAGGTGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCC‑ATATCCACATTGTC‑GCCATTGCTCCGG‑GATCTTCTGAACGCTCAAtctc                                                                                                                                                                                         >  1:375278/4‑251 (MQ=255)                                                   gtaTCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGACGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGGACGGCTGGCCATTATCTCGGTGGTAGGCGATG‑GTATGCGCACCTTTCGGGGGGTCCCCGCGGAAATCTTTGGCGCGCTGGCCCCCGCCA‑ATATCAACGTTTTC‑GCCCTTGCTCCGG‑GGTCTTCTggctgaccaatctct                                                                                                                                                                                        >  2:202015/3‑237 (MQ=255)                                                          tttCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGGGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTgg                                                                                                                                                                                 >  1:420413/1‑251 (MQ=255)                                                           ttCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGACGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGCGATG‑GGATGCGCACACTGCGTAGGATCTCCGCGCAATTTTTTGCCCCGCAGGCCCGCGCCC‑ATATCAACATTTCC‑GCCCTTGCCCAGG‑GACCTTCTGAACGCACACTCTCCGTCGTggg                                                                                                                                                                                >  2:253589/1‑250 (MQ=255)                                                               gCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑Taaa                                                                                                                                                                            <  1:202015/250‑1 (MQ=255)                                                                     cgcaAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAATAGTTCTACCTGGAACTGAAAGAAGGCTTACGGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACgat                                                                                                                                                                     <  1:233340/249‑1 (MQ=255)                                                                            cGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGGCGGAAAGGCTGGCCATTATCTCGGTGGTAGGTGATG‑GGATGCGGACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTTTC‑GCCATTTCTCCGG‑GATCTTCTGAACGCTCAATATCTGTCGTGG‑TAAAAAACGATGATGCg                                                                                                                                                               >  1:356202/1‑250 (MQ=255)                                                                             gACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCATG‑GATCTTCTGAACGCTCAATCTCTGTCGTGGGTAAA‑AACGATGATGCGAc                                                                                                                                                             >  1:109989/1‑251 (MQ=255)                                                                             gACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGGGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGATGATGCGAc                                                                                                                                                             >  1:348264/1‑251 (MQ=255)                                                                              aCTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGATGATGCGAcc                                                                                                                                                            <  1:357840/251‑1 (MQ=255)                                                                                    ggCGAGCTGAACGGGCAATGCAGGAAGATTTCTACCTGGAACGGAAAGAAGTCTGACTGGAGCCGCGGGCAGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATG‑GTATGCGCACCTTGCGTGGGATCGCGGCGAAATTCTTTGCCGCACTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGATGATGCGACCACTGGc                                                                                                                                                      <  2:422321/250‑1 (MQ=255)                                                                                     gCGAGCTGAACGGGCAATGCAGGAAGAGTGCTACCTGGAACTGAAAGAAGGCTTACGGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGAGGATGCGACCACTGGc                                                                                                                                                      <  2:288402/250‑1 (MQ=255)                                                                                       gAGCTGAACTGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGGAGGTGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCTATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGATGATGCGACCACTGGCGTg                                                                                                                                                   <  1:96304/251‑1 (MQ=255)                                                                                                 ggCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGGTG‑GTATGCGCACCGTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCCTTGCTCAGG‑GATCTTCTGAACGCCCAATCTCTGTCGTGG‑TAAATAACGATGATGCGACCACTCGCGTGCGCGTTACtt                                                                                                                                         >  1:616517/1‑250 (MQ=255)                                                                                                      ggCAGGAAGAGTTCTACCTTGAACGGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCACTATCTCGGTGGTAGGTGATG‑GTATGCGCTCCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGATGATGCGACCACTGGCGTGCGCGTTACtcatca                                                                                                                                     <  2:10537/249‑1 (MQ=255)                                                                                                               agTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGGGATG‑GGATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTggtc                                                                                                                           >  1:510813/1‑248 (MQ=255)                                                                                                                   atcccTGGAACTGACAGAAGGCTTATTGGAGGCGGTGGTGGGGACGGAACGGGTGGGCTTTATCTCGGTGGTAGGGGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCGAAAGTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACAGTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTTATCAGATGTTGTTCAAt                                                                                                                        <  2:606164/247‑1 (MQ=255)                                                                                                                    tACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGGGGTAGGGGGTG‑GTATGCGCAACTTGCGGGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GGTCTTTTGAACCCACCATCCCTGTCGGGG‑TAAATAACGCTAATGCGACCACTGGCGTGCGCGGTTCtcatccaggtcttgtcaatac                                                                                                                      >  2:156721/1‑235 (MQ=255)                                                                                                                     aCCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCGCGGTGGTAGGTGATG‑GTATTCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATAc                                                                                                                      <  1:428889/250‑1 (MQ=255)                                                                                                                          gAACTGAAAGAAGGATTACTGGAGCCGCTGACAGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGAAG‑GTATGCGCACCCTGCGTGGGATCTCGGCGAAATTCTTTGCCGCACTGGCCCGCTCCA‑ATATCAACATTGTA‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGATGATGCGACCACTGGCGTGCGCGTTAATCATCAGATGCTGTTCAATACCGATc                                                                                                                 >  1:345450/1‑250 (MQ=255)                                                                                                                                  agaagGCTTCTTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCg                                                                                                        <  2:93488/251‑1 (MQ=255)                                                                                                                                  agaagGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCg                                                                                                        <  1:13668/251‑1 (MQ=255)                                                                                                                                     agGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGGAGGGGATG‑GCATGCGCACCTTGGGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GGTCTTCTGGACGCCCAATCTCTGTCGTGG‑GAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGATGGTCAATACCGATAGGGTTATCGa                                                                                                       >  1:289873/1‑249 (MQ=255)                                                                                                                                           aCTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGttt                                                                                                <  1:439439/250‑1 (MQ=255)                                                                                                                                           aCTGGAGCCGCTGGCGGGGACGGAACGGCTGGCCATTATCTCGGTGGTCGGTGCTG‑GGATGCGCCCCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCC‑ATATCAACATTTTC‑GCCCTTGCTCAGG‑GTTCTTCTGAACGCCCACTCTCTGTCGGTG‑TAAATAACGCTGATGCGACCAATGGCGGGCGCGTTACTCATCAGGTGCTGTTCAATTCCGGTCCGGTTATCGGAGTttttt                                                                                               >  2:343543/1‑250 (MQ=255)                                                                                                                                             gggtgCCGTTGGCGGTGACGGAACGGCGGGCCATTATCTCGGTGGTAGGTGATG‑GTATGCGCACCTTGCGTTGGATCTCGTCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGAGCAGGTTATCGAAGTGTTtgtg                                                                                             <  2:109989/247‑1 (MQ=255)                                                                                                                                              ggAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGa                                                                                            <  1:87618/251‑1 (MQ=255)                                                                                                                                                       ggcggTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCg                                                                                   <  1:110947/247‑1 (MQ=255)                                                                                                                                                          ggTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGT‑GATCTTCTGAACGCGCAATCTCTGTCg                                                                                                                                                                                    <  2:290224/151‑1 (MQ=255)                                                                                                                                                          ggTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCg                                                                                                                                                                                    >  1:290224/2‑152 (MQ=255)                                                                                                                                                               cGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCAGCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGtt                                                                           <  1:649062/251‑1 (MQ=255)                                                                                                                                                                 gAACGGCTGGCCATTATCTCGGTGGTAGGTGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGCAGTGTTTGTGATTGGCGTCGGTGGCGTTgg                                                                         >  1:34004/1‑251 (MQ=255)                                                                                                                                                                 gAACGGCTGGCCATTATCTCGGTGGTAGGTGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTgg                                                                         <  2:348264/251‑1 (MQ=255)                                                                                                                                                                                        ggtAGGTGATG‑GTATTCGCTCCGTGCGTGGGAGCGCGGCTAAATTCTTGGCCGCTCAGGCCCTCGCCA‑CTATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGGCGTGG‑TTAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTAGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCatcgga                                                  <  2:193722/251‑6 (MQ=255)                                                                                                                                                                                           aGGTGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCCTCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTTCTGGAGCCACTGAAgg                                               >  2:667552/1‑250 (MQ=255)                                                                                                                                                                                              tGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGGTCAAACCCGAGCAGGTTATCGAAGGGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGAGGCAAATGAAGCGTc                                            >  1:634879/1‑251 (MQ=255)                                                                                                                                                                                              ggATG‑GTATGCGCCCCTTGCGGGGGAGCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGGC‑GCCATTGCTCAGG‑GATCTGCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTGCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTc                                            <  2:241550/250‑1 (MQ=255)                                                                                                                                                                                                 ggtgTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCaa                                          <  2:552754/248‑2 (MQ=255)                                                                                                                                                                                                  g‑gTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTTCGCTGCTGGAGCAACTGAAGCGTCaaca                                        <  1:510935/251‑4 (MQ=255)                                                                                                                                                                                                  g‑gTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCaac                                         <  1:343543/250‑3 (MQ=255)                                                                                                                                                                                                    gTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGATGATGCGACCACTGGCGTGCGCGCTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCaaca                                        >  1:301528/1‑247 (MQ=255)                                                                                                                                                                                                        gcgatgctgtggggggATCTCGGCGAAATGTGGTGGCGCGCTGGCCGGCGCGA‑ATAGCAGCAGTGTC‑GCTATTGCGCAGG‑GATCTTAGGAACGCTCAATGTCTGTCGTGG‑GAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGAGCAGGTTATCGAAGTGTTTGTGATGGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCaataaagca                                   <  2:321352/238‑9 (MQ=255)                                                                                                                                                                                                           cACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGacgaagactgacgctcaggtgcgaa                                  >  1:590568/1‑225 (MQ=255)                                                                                                                                                                                                            ccctttcgggggATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCGCAATCTCTGTCGTGG‑TAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAGACCGATCAGGTTATCGAAGTGGTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTTAAGCGTCAACAAAgctggct                               <  2:319608/242‑1 (MQ=255)                                                                                                                                                                                                            aCCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGACCAGGTTCTCGAAGGGTTTGTGATTGGCGTCGGGGGCGGTGGCGGGGCGCTGCGGGAGCCACTGACGCGTAAACAAAgctggct                               >  2:17787/1‑251 (MQ=255)                                                                                                                                                                                                              cTTGCGTGGGATCTCGGCGAAATTCTTTGCAGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGATGATGCGACCACTGGCGTGCGAGTTACTCATCAGATGCTGTTCAATACCGATCAGGATATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAAATGAAGAGTCAACAAAgctggctg                              >  1:504413/1‑250 (MQ=255)                                                                                                                                                                                                                 gCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTgaag                           <  2:504413/250‑1 (MQ=255)                                                                                                                                                                                                                  cgggtgATCTCGGCGAAATTCTTTGCCGCGCGGGCCCGCGCCA‑ATATCAACATCGTC‑GCCATTGCTCAGG‑GAGCTGCGGGACGCTCAATCTCTGTCGTGG‑TAAATAACGAAGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCTATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTgaagaa                         <  2:198460/246‑1 (MQ=255)                                                                                                                                                                                                                   gTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAAt                        >  1:471246/1‑251 (MQ=255)                                                                                                                                                                                                                      ggATCTCGGCTAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCGCAATCTCTGGCGTGG‑TAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATaaa                     <  2:510813/251‑1 (MQ=255)                                                                                                                                                                                                                            cGGCGAAATGCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATGGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACata                 <  1:278449/249‑1 (MQ=255)                                                                                                                                                                                                                              gCGAAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCGa             <  1:268561/251‑1 (MQ=255)                                                                                                                                                                                                                                      cTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCGACTTACgtg     >  1:648993/1‑251 (MQ=255)                                                                                                                                                                                                                                         tGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCt  >  1:163702/1‑251 (MQ=255)                                                                                                                                                                                                                                         ggCCGCGCTGGCCCGCGACC‑ATTTCAACATTGTC‑GCCATGGCTCAGG‑GATCTTCTTAACGCTCTATCGCTGGCGTGG‑TAAATAACGAGGATTCGACCACTGGCGTGCGCGTTACTCATCAGAGGCTGTTCAATGCCGATCAGGTTAGCGAAGTGTTTGTGAGTGTCGCCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAATCTTCAACAAAGCTGGCTGAAGAAGAAACATATCGACTTACgtggca  <  2:24906/250‑4 (MQ=255)                                                                                                                                                                                                                                          gCCGCGCTGGCCTGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCGCAATCTCTGTCGTGG‑TAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCt  <  2:471246/250‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                         ACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCACTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCT  >  NC_000913/1356‑1837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑