Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	15,073	A→T	L302L (CTA→CTT)	dnaJ →	chaperone protein DnaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	15,073	0	A	T	94.8%	221.9 / 4.1	78	L302L (CTA→CTT)	dnaJ	chaperone protein DnaJ
Reads supporting (aligned to +/- strand):  ref base A (1/3);  new base T (39/34);  total (40/38)
Fisher's exact test for biased strand distribution p-value = 3.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-02

ACTGGAGACCGCATCCGTCTT‑GCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTATTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCC  >  NC_000913/14831‑15304                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                        acTGGAGACCGCATCCGTCTT‑GCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTAt                                                                                                                                                                                                                                 <  1:231310/251‑1 (MQ=255)   tGGAGACCGCATCC‑TCTTTGCGGGTTAAGTTGATGCGGGTGAGCATGTCGCACCGGTAGGCGTCCTGTAAGTGCAGGTTCAGGTTAAACAGCACCCGATTTTCGCGCGTGAAGGCAATATCCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTTGGTGGCGAAATCGAAGTACCGATCCTTGAGGGTCGCGTCAAACTGAAAGTGCCTGGCGAACCCCAGACCGGTAAGCTTTTCCGTATg                                                                                                                                                                                                                                <  2:581874/250‑1 (MQ=255)           gCATCCGTCTT‑GCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTaa                                                                                                                                                                                                                        >  1:157971/1‑250 (MQ=255)             aTCCGTCTT‑GCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAgg                                                                                                                                                                                                                     >  1:129217/1‑251 (MQ=255)             aTCCGTCTT‑GCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAgg                                                                                                                                                                                                                     <  1:65965/251‑1 (MQ=255)               ccGTCGT‑GCGGGCGAAGGTGAAGCGGGCGAGCATGTCGTACCGGCAGGCGAGCGGTACGTTCAGGTTCAGGTTAATCAGCACCCGATTGTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGGGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCTTATGCGCGGTAAAGGc                                                                                                                                                                                                                    <  1:538713/250‑1 (MQ=255)                 gTCTT‑GCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTc                                                                                                                                                                                                                 >  1:190867/1‑251 (MQ=255)                        cggtcgatgttggaGCGGGTGAGCATGGCGCACAGGCAGTCGATCTGTACGTTCAGGTTCAGGTTAAACTGCTCCCGATTTTCGAGCGTGAAGGCAACAACCTTTATTGCTAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGATATCGAAGTACCGTCCCTTGATGGTCTCGTCAAACTTACAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTca                                                                                                                                                                                                           <  2:434790/238‑2 (MQ=255)                          ggCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGAACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGAGAAACTGAAAGGGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCACTGTAAAGGGGTCAAGTCTGt                                                                                                                                                                                                         >  1:156726/1‑251 (MQ=255)                              aaGGAGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTTTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCAAGCGTGAAGGCAACAACCTCTATTGAGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGAGGGGCGAAATCGAAGTACAGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGACAACCAGACCGGTAAGCTTTTCTGAATACGCGGTAAAGGCGACAAGtattgcc                                                                                                                                                                                                       >  2:450551/1‑243 (MQ=255)                               aGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGGAAAGGCGTCAAGTCTGTCcgcg                                                                                                                                                                                                    >  1:170456/1‑251 (MQ=255)                                   gAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTg                                                                                                                                                                                                 <  1:167525/250‑1 (MQ=255)                                            gAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAggg                                                                                                                                                                                       <  1:56873/251‑1 (MQ=255)                                                     gcACCGGCAGGCGTTCTGTACATTCAGGTTCAGGTGAAACAGCGCCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGTCGAAATCGAAGTACCGACCCTTGAGGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCTGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGGCTGTCCGCGGTGGCGCACAGGGTGATTTGCt                                                                                                                                                                              <  2:662648/251‑1 (MQ=255)                                                       aCCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCtgt                                                                                                                                                                            <  1:27150/251‑1 (MQ=255)                                                            cAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCcgc                                                                                                                                                                       >  1:152633/1‑251 (MQ=255)                                                                   tCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTgtcg                                                                                                                                                                >  2:346947/1‑251 (MQ=255)                                                                      ttACGTTCAGGTTCAGGATAAACAGCACCCGATATTCGAGCGTGCAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGAGGCGCAAACGAAGTACCGACCCTTGATGGTCGAGTCAAAATGAAAGTGCCATGCGAAACCCAGACCGGTGAGCTTTTCCGACTGCGCGGTACAGGCGTCAAGTCTGTCAGCGGAGGCGCACAGGGTGATTTGCTATGCCACGATGtcgtcg                                                                                                                                                             >  2:668826/2‑251 (MQ=255)                                                                      gTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTgtcg                                                                                                                                                                <  1:167744/248‑1 (MQ=255)                                                                      gTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTgtc                                                                                                                                                                 <  1:30312/247‑1 (MQ=255)                                                                                     tgttAAACAGCACCAGATTTTAGAGCGTGAATGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGAACCGACCATTGGTGGTCGCGTCAAACTGAAAGTGCATTGCGAAACCCCGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGAGGGCGCACAAGGTGATTTGCTACTCCGCGTTGTCGTCGACACACCGGCAGGcc                                                                                                                                              >  2:93929/2‑251 (MQ=255)                                                                                       ttAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTTGCGAAATCGAAGTAACGACCCTTGCTAGGCGCGTCCAACTGAAAGGGCCTGGCGCAACCCAGACCGGTAAGCTTTTACGGATGCGCGGGAACAGCGTCAAGTCTGTCCGCGGTTGCGCACAGGGGGATTTGCTGTGCCGTGTCTTTGACGAAAACCCGGTAGGCCTg                                                                                                                                            >  2:451476/1‑251 (MQ=255)                                                                                          aaCAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAAc                                                                                                                                         <  1:650812/251‑1 (MQ=255)                                                                                             aGCAACCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGAGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGGGGCGGAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTAAAAGTGCCTGGCGAAACCCAGACCGGTAAACTTTTCCGGATGCGCGGTAAAGGCGTCCAGTCTGt                                                                                                                                                                                                         >  1:175060/1‑184 (MQ=255)                                                                                               cccccGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGTTGTCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGagaa                                                                                                                                    <  2:367476/249‑4 (MQ=255)                                                                                                    gATTTTCGAGCGTAACGGAAACAACCTATATTGCGAAGTCCCGCTCAACTTCGCTATGG‑CGGCGATGGGCGGCGAAATCGAAGTACCGCTCCTTGATGGTCGCCTCCACCTGGAACTGCCTGGCGCACCCCAGACACGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGCATGTCCGCGGTGGCGCCCAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCgatctgccttagcacgaagcagg                                                                                                                               >  2:40183/1‑229 (MQ=255)                                                                                                     aTTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGGGCACAGGGTGATTTGCTGTTCCGCGGTGTCGTCGAAACACCGGTAGGCCTGACCGagaaggaga                                                                                                                               >  2:189796/1‑242 (MQ=255)                                                                                                      ttttCGAGCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTATTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAAAGGCAGaa                                                                                                                              >  2:238270/1‑250 (MQ=255)                                                                                                      ttttCGAGCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGG‑AGGCGCTGGGGGGCGAAATCGGAGTACCGACCCTTGATGGTCGAGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCCTTTCCGTATGAGCGGCAAAGGGGTCAATGCTGTCCGCGGTGGCGACCCGGGGGATTTGCTAGACCGCGTTTTACTCAAAACACCGGTAGGCCTGAACgtgaagcagac                                                                                                                              >  2:199035/1‑240 (MQ=255)                                                                                                      ttttCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTgtcg                                                                                                                                                                <  1:3910/216‑1 (MQ=255)                                                                                                         tCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAATGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTATGCCTGAAc                                                                                                                                         >  1:108699/1‑236 (MQ=255)                                                                                                         gcGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCGGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGGCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAAc                                                                                                                                         <  2:108699/235‑1 (MQ=255)                                                                                                            aGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTATCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAgctg                                                                                                                       <  1:146683/251‑1 (MQ=255)                                                                                                             gCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAgctgc                                                                                                                      <  1:163833/251‑1 (MQ=255)                                                                                                                 gAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGGAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCCCAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAAAAGCtgctgc                                                                                                                   >  2:345227/1‑250 (MQ=255)                                                                                                                   aGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTgctgca                                                                                                                  >  1:591745/1‑249 (MQ=255)                                                                                                                      caacaaCCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAg                                                                                                                                                 <  2:536369/215‑1 (MQ=255)                                                                                                                      caacaaCCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGGGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAg                                                                                                                                                 >  1:536369/1‑215 (MQ=255)                                                                                                                           acCTCTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGAAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTCGGCCTGCACGAGTAGCAGAAACAGCTgctgcaagagctgcc                                                                                                         >  1:375234/1‑249 (MQ=255)                                                                                                                            ccTGTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTATTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAAAGGCAGAAACAGCTgctgcaagagctgcaag                                                                                                       <  1:351149/251‑1 (MQ=255)                                                                                                                                tATTGCGAAGTCCCGATCAACTTCTCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGCAAGCTTTTCCGTATGCGCGGTACAGGCGTCCAGTCTGTCCGCGGTGGCGCCCAGGGTGCTTTGCTGTGCCGCGTTGTCGTCACAACACCGGTCGGCCTGAACGAGAAACAGAAACAGCTTCTGCAAAAGATGCAAGaa                                                                                                     >  2:18489/1‑249 (MQ=255)                                                                                                                                tATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGCCCCTTGATGGTCGCGTCAAACTGAAAGGGCCTGGCGCACCCCAGACCCGGAAGCTTTTCCGGATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCCCAGGGGGATTTGCTGTGCCGCGTTGTCGTCGACCCACCGGCAGGCCTGAACGAGAAGCAGAGAAAGCTGCCGCAAAACCCGCAAGaaa                                                                                                    >  2:463549/1‑250 (MQ=255)                                                                                                                                      gAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGGCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTATGTCCGCGGGGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGCGAAGCAGAAACAGCTGCTGCCAGAGCGGCCAGAACACTTCgg                                                                                             >  2:582369/1‑251 (MQ=255)                                                                                                                                        aGTCCCGATTAACTTCGC‑ATGGGCGGCGCTGGGGGGCGAAATCGAAGTACCGACCCTGGAGGGTCGCGTCAAACTGAAAGTGCCTGGCTAAACCCAGACCGTTAAGCTTTTCCGTATGCGCGGTAAAGGCGGCAAGTCGGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCTGTGTCTTCGAAACACCGGTAGGCCTGCACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTGCGGTg                                                                                           <  2:375234/251‑1 (MQ=255)                                                                                                                                         gTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTgg                                                                                          <  1:625610/251‑1 (MQ=255)                                                                                                                                           cccGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGcc                                                                                        >  1:420768/1‑251 (MQ=255)                                                                                                                                           cccGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAAATGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAACGCTTCGGTGGcc                                                                                        >  1:447708/1‑251 (MQ=255)                                                                                                                                               agcAACTTCGCGTTGG‑CGGCGCTGGGTGGCGAAACCTAAGTACCGCCCCTTGGTGGGCGTGTCAAACTGAAAGTGCCGGGCGAACCCCAGACCGGTAAGCGTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGGCCGCGGTGGCGCACAGGGTGATTTGATGTGTCGCGTGGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAAc                                                                                    <  2:146368/249‑1 (MQ=255)                                                                                                                                                   aCTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCggt                                                                                <  1:18489/251‑2 (MQ=255)                                                                                                                                                          tATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTa                                                                                                                                                  <  1:230366/178‑1 (MQ=255)                                                                                                                                                           aTGG‑CGGCGCTGGGGGGCGAAATCGAAGTAACGAACCTTGATGGTCGCGTCAAACTGAAAGTGCCTGACGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTATGTCCGCGGGGGCGCACAGGGAGAATTGCTGTGCCGCGTTGTCGTCGAAACACCGGGAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGAAAGAAAGCTTCGGGGGCCCAAACGGTGAGccca                                                                         >  2:444427/1‑247 (MQ=255)                                                                                                                                                              ggcgtgggtgtgTGGCGCAACCGAAGTAACGACCCTTGATGTGCGCGTCAAGCTGATCGTGCCGGGCGAAACCCAGCCCGGTAAGCTGTTCCGTATGCGCGGTAAAGTCGGCAAGTCTGTACGCGGTGGCGCCCTGGGTGATTTGCTTTGCCGCGTTGTCGGCGAAACACCGGTAGGCCTGAACGAGAATCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTTGCCCATCAGGTGAGCacaaca                                                                      <  2:43044/240‑1 (MQ=255)                                                                                                                                                                           ggCGAAATCGAAGGACCGCCCCTTGATGGTCGCTTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAGGGCGTCAAGTCTGTCCGCGGTGGTGCACAGGGTGATTTGCTGTGCCGCGTTGTCGGCGAAACACCTGTAGACCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGTTTAGCACAACAGCCCGCGCTCaa                                                          <  2:386906/250‑1 (MQ=255)                                                                                                                                                                                   cGAATTACCGACCCCTGATGGTCGCGTCAAACTGAAAGCGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGTTAAAGGCGTCAAGTCTGGCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGcttc                                                  <  2:573966/250‑1 (MQ=255)                                                                                                                                                                                   cGAAGTACCGACCCCTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGGCCGGTAAGCTTTGCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTGGCTGTGCCGCGGTGGCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGcttct                                                 <  2:532365/251‑1 (MQ=255)                                                                                                                                                                                      aGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTATTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTg                                              <  1:199035/251‑1 (MQ=255)                                                                                                                                                                                      aGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTATTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTg                                              <  1:238270/251‑1 (MQ=255)                                                                                                                                                                                          ccGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCCAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCCTTGATgg                                          >  2:392563/1‑251 (MQ=255)                                                                                                                                                                                           cGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAACGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGCGCTTCTTTGCTggg                                         >  2:53315/1‑250 (MQ=255)                                                                                                                                                                                           cGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGgt                                         >  1:429318/1‑251 (MQ=255)                                                                                                                                                                                            gACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCGGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGgtg                                        >  1:140686/1‑251 (MQ=255)                                                                                                                                                                                            gACCCTTGATGGACGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTATGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGGACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGAGAGCACAACAGCGCGCGCTCAAAGCGCTTCTTTGAGGgtg                                        >  2:469343/1‑251 (MQ=255)                                                                                                                                                                                             aCCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGgtgt                                       <  1:457498/251‑1 (MQ=255)                                                                                                                                                                                                  tGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTCGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTgaa                                    <  1:450551/249‑1 (MQ=255)                                                                                                                                                                                                      ggTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGtt                              <  2:157971/251‑1 (MQ=255)                                                                                                                                                                                                        gcgcgTCAAACTGAAAGTGTCTGGTGAAATCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGGCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGATCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGttt                             <  2:44961/249‑1 (MQ=255)                                                                                                                                                                                                         cgggTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGttttt                           <  2:187374/248‑1 (MQ=255)                                                                                                                                                                                                         cgcgTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGttttt                           >  1:276008/1‑251 (MQ=255)                                                                                                                                                                                                           cgTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAAAAGCCCGCGCTCAAAGAGCTTCGTTGATGGTGTGAAGAAGTTTTTTg                         >  2:432761/1‑251 (MQ=255)                                                                                                                                                                                                                 accgAAAGTGCATGGCGAAACCCAAACCGGTAAGCTTTTCCGAATGCTCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACCGGGTGATTTGCTGTGCCGCGTTGACGCCGCAACACAGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGCGCTGCACGTAAGATTCGGTGGACAAACGGGTGAGAACTACAGACCGTGCACAACGAGCTTATTTGATGGGGATAAGGAGTTATTTGACGAcc                   >  2:517157/4‑251 (MQ=255)                                                                                                                                                                                                                   tGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCaaa                                                         >  1:465769/1‑211 (MQ=255)                                                                                                                                                                                                                         tGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTACAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAACCAGCTGCTGAAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGGGGGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGCTGGGGTGGAGCAGGTTTTTGAAGACCTTACCCGc           >  2:134636/1‑251 (MQ=255)                                                                                                                                                                                                                          gCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCt          >  1:673448/1‑251 (MQ=255)                                                                                                                                                                                                                              ggCGAAACCCAGACCGGTAAGCGTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTaa        <  1:310865/249‑1 (MQ=255)                                                                                                                                                                                                                               gCGAAACCCAGACCGTTAAGCTTTTCCGTATGGGCGGTAAAGGCGTCAAGTCGGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCt     <  2:190867/251‑1 (MQ=255)                                                                                                                                                                                                                                  aaaCCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAGGAGCTTCTTTGATGGCGTGAAGAAGTTTTTTGACGCCCTGACCCGCTAACCTccc  >  2:146516/1‑251 (MQ=255)                                                                                                                                                                                                                                       cAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTgctgca                                                                                                                  >  2:481032/1‑134 (MQ=255)                                                                                                                                                                                                                                       cAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTgctgca                                                                                                                  <  1:481032/134‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                        ACTGGAGACCGCATCCGTCTT‑GCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGG‑CGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTATTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCC  >  NC_000913/14831‑15304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑