Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	14,701	T→C	A178A (GCT→GCC)	dnaJ →	chaperone protein DnaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	14,701	0	T	C	98.5%	215.0 / ‑4.5	68	A178A (GCT→GCC)	dnaJ	chaperone protein DnaJ
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (28/38);  total (29/39)
Fisher's exact test for biased strand distribution p-value = 4.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01

CGGCGATATTTTTGGCGGCGGACGTGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACTTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGCCAGGGATTCTTCGCTGTACAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCG  >  NC_000913/14461‑14940                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 cGGCGATATTTGTGGGGGCGGACGTGGTCGTCACCGTGCGGCGCGCGGTGCTGATTTCCGCTAGAACATGTAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGGCCAAAGAGAGCCGCATTCCGACTCTGGAAGAGTGTG‑ACGGTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGGCATGGTTCGGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTTcagc‑ag                                                                                                                                                                                                                                        <  2:445905/250‑1 (MQ=255)    cGATATTTTTGGCGGCGGACGTGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCt                                                                                                                                                                                                                                    <  1:353713/251‑1 (MQ=255)             tGGCGGCGGACGTGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCacac                                                                                                                                                                                                                            <  1:89264/250‑1 (MQ=255)                   gggACGTGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGTATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAgg                                                                                                                                                                                                                     <  1:206518/250‑1 (MQ=255)                      gcGTGGGCGGCAACGGGGGGCGCGTGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGTCCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACTCTGTCAGGGcc                                                                                                                                                                                                                  <  2:477125/250‑1 (MQ=255)                       cGTGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCcg                                                                                                                                                                                                                 <  1:277735/251‑1 (MQ=255)                        gTGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCcgc                                                                                                                                                                                                                >  1:43044/1‑251 (MQ=255)                             cgtcAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTa                                                                                                                                                                                                            <  1:379560/250‑1 (MQ=255)                                 aaCGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTg                                                                                                                                                                                                       >  1:137006/1‑251 (MQ=255)                                         gcgcgcGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGa                                                                                                                                                                                               <  1:119651/251‑1 (MQ=255)                                            cgcgGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGCAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACACTGATCAACGATcc                                                                                                                                                                                            >  2:229076/1‑251 (MQ=255)                                              cgGTGCTGATTTACGCTATAAAATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCTTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCGGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGt                                                                                                                                                                                          <  2:279058/251‑1 (MQ=255)                                                  gCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGca                                                                                                                                                                                       <  1:437208/250‑1 (MQ=255)                                                       tttACACTATAACCTGGAGCTAACCCTCGAAATCGCTGTCCGTGGCGCGACCACAGAGATCCGCATTCCGACTCTGGGAGAGTGTG‑ACGTTTGCCACGGCAGCGGTGCACAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGACGTGCAGCCAG‑CCTGTCAACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAAt                                                                                                                                                                                 >  2:668427/1‑251 (MQ=255)                                                          aCGCTATAACATGGAGCTTACCCTCGAAGAAGCTGTACGTGGCGGTACCAAAGAGATCCGCATGCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATgt                                                                                                                                                                               <  2:678971/250‑1 (MQ=255)                                                           cGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATgtca                                                                                                                                                                             <  1:439230/251‑1 (MQ=255)                                                            gCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCTGTGCAACAAATgtcat                                                                                                                                                                            <  1:514616/251‑1 (MQ=255)                                                             cattaACATGGAGCTCATCCTCGCATAAGCGTTACGTGGCGTGAACAAATAGATCCGCATTCCGACCCTGTAAGTGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCTACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCAGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGGGCAACAAATgtcatg                                                                                                                                                                           <  2:150789/248‑1 (MQ=255)                                                                    aTGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGtcatggtcatgg                                                                                                                                                                    >  1:629722/1‑251 (MQ=255)                                                                      ggAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGAGCCGCATTCCGACTCTGGAAGAGTGTG‑ACGGTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTGTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTcatggtcatggt                                                                                                                                                                   <  1:93515/250‑1 (MQ=255)                                                                           tCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGTTCACAGAt                                                                                                                                                                                              >  2:188067/1‑218 (MQ=255)                                                                           tCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGAt                                                                                                                                                                                              <  1:188067/218‑1 (MQ=255)                                                                           ccACCCTCGAATAAGCTGTACGTGGCGTGACCAAAGAGAGCCGCATTCCGCATCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGTTGCAAAACCAGGTACACAGCCGCAGACCGGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCGGGGCCGCGGTACGCTGATCAAAGATCCGAGCAACAAATGGCATGGTCATGGTCGTGtt                                                                                                                                                             <  2:22600/250‑1 (MQ=255)                                                                                  cGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAgcg                                                                                                                                                        <  1:594888/249‑1 (MQ=255)                                                                                  cGAAGAAGCTGGACGGGGCGTGACCAAAGAGATCCGCATTCCGACTCTTGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGAACTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTGGAGCgca                                                                                                                                                      <  2:286495/251‑1 (MQ=255)                                                                                     agaagCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCgcagc                                                                                                                                                    >  1:94133/1‑250 (MQ=255)                                                                                     agaagCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGAACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGAgcagg                                                                                                                                                    >  2:474798/1‑249 (MQ=255)                                                                                              aCGTGGCGTGACCAAAGAGATCCGTATTCCGACTCTTGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTTTCAGGGCCGCGGTACGATGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCATCAAAACGCTGt                                                                                                                                          <  2:393207/251‑1 (MQ=255)                                                                                                  ggcggtaCCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTATCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCGGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGt                                                                                                                                      <  2:648939/245‑1 (MQ=255)                                                                                                       gACCAAAGAGTTCCGCATTCCGACTCTGGAAGGGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTTGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTaaa                                                                                                                                  <  2:14059/250‑1 (MQ=255)                                                                                                         ccAAAGAGATCCTCATTACGATTCTGGCAGAG‑GTGTACGTTTGCCTCGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGGCATGGGTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGGCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATc                                                                                                                               <  2:449685/251‑1 (MQ=255)                                                                                                                gaTCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGtt                                                                                                                                                             >  2:594568/1‑214 (MQ=255)                                                                                                                gaTCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGtt                                                                                                                                                             <  1:594568/214‑1 (MQ=255)                                                                                                                    cGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAggggg                                                                                                                    >  1:36208/1‑250 (MQ=255)                                                                                                                        ttCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACCAATGTCATGGTCATGGTCGTGTTGAGCGAAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGac                                                                                                                >  1:434790/1‑251 (MQ=255)                                                                                                                        ttCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCTTGTTGAGCGCAGCAAAACGCGGTCCGTTAAAATCCCGGCAGGGGTGGac                                                                                                                >  1:434745/1‑251 (MQ=255)                                                                                                                          ccGACTCTGGAAGAGTGTG‑ACGTTTACCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTCAAATCCCGGCAGGGGTGGacac                                                                                                              >  1:107125/1‑251 (MQ=255)                                                                                                                             aCTCTGGAAGAGGGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTAAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGACGCGGTACGCTGATCAAAGATCCGTGCAACAACTGTCATGGGCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGGGGGCACTgg                                                                                                           >  2:494426/1‑251 (MQ=255)                                                                                                                                     agagTGTG‑ACGTTTGCCACGGAAGCGGAGCAAGACCAGGTACACAGCCGCAGACTTGGCCGACCTGTAATGGTTCTGGTCAGGTGCAGATGCGCCAGGGATTCTTCGCTGAACAGC‑AAACCTGTCCACACTGTCAGAGCCGCGGTACGCTGATCAAAGACCCGTGCAACAAATGTCATGCTCATGGTCGTGTTGCGCGCACAAAAACGCTATCAGGTAAAATCCCGGCGGGGGTGGACCCAGGCGACCGcc                                                                                                   >  2:351149/1‑250 (MQ=255)                                                                                                                                        gtgtG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGGCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGTACACTGGAGACTGCATcc                                                                                                <  2:94133/251‑1 (MQ=255)                                                                                                                                          gtg‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGt                                                                                              >  1:581874/1‑251 (MQ=255)                                                                                                                                            g‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCt                                                                                            >  1:367476/1‑251 (MQ=255)                                                                                                                                              aCGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACCAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACCATGGGGACCGCATCCGTCtt                                                                                           >  1:532170/1‑251 (MQ=255)                                                                                                                                               cGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGACGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTg                                                                                          >  1:549574/1‑251 (MQ=255)                                                                                                                                                   tGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGAGGGAAACTGGAGACCGCATCCGTCTTTCggg                                                                                      >  2:642351/1‑251 (MQ=255)                                                                                                                                                      cACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCg                                                                                    >  1:512015/1‑250 (MQ=255)                                                                                                                                                         ggTAGCGGTGCAAAACAAGGCACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCAGCGGTACGCTGATCAACGATCCGTGCAACACATGTCATGGTCATGGTCGTGTTGAGCGCAGCTAAACGCTGTCCGTTAAAATCCCGGCAGGGGGGGACCCTGGAGAcgacatgcggattgaggtcgacgg                                                                                >  2:310865/1‑228 (MQ=255)                                                                                                                                                             gCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAAGCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGaa                                                                            <  1:406188/251‑1 (MQ=255)                                                                                                                                                                  gCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCggg                                                                       <  1:132665/251‑1 (MQ=255)                                                                                                                                                                  gCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCgg                                                                        <  1:561286/250‑1 (MQ=255)                                                                                                                                                                      aaCCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTACTGGGCAGGTGCAGATGCGTCAGGGATTCGTTGCCGTGCAGC‑AGACCGGTCCACACTGTCAGGGCCGCGGTACGCTGAACAAAGATCCCTGCAACAAATGTCATGGTCATGGGCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTCGACACTGGAGACCGCATCCGTCTTGCGGGc                                                                                     <  1:257726/233‑1 (MQ=255)                                                                                                                                                                             gacacAGCCGCAGACCTGGCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGgcg                                                            <  1:429653/250‑1 (MQ=255)                                                                                                                                                                              acacAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGAGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AACCCTGTCCACACTGTCAGAGCCGCGGTACGCTGATAAAAGATCCGTGCAACAAATGTCATGGACATGGTCATGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATACCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGGAGGAGAAGCGGGCGTGCATGgcgc                                                           >  1:630821/1‑251 (MQ=255)                                                                                                                                                                                           cctGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGAt                                              >  1:44961/3‑251 (MQ=255)                                                                                                                                                                                           cctGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGAt                                              <  1:370282/249‑1 (MQ=255)                                                                                                                                                                                             tGTCCGACCTGTCCTGGCTCTGGACCGGTGCAGCTGCGTCAGGGCTTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGCTCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGCCCGCCTCCGTCTTGCGGGCGAAGGTACAGCGGGCGCGCATGGCGAACCGGCAgggcatcg                                            >  2:44897/1‑245 (MQ=255)                                                                                                                                                                                                ccGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTa                                         <  1:306945/251‑1 (MQ=255)                                                                                                                                                                                                 cGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTAc                                        <  1:552910/251‑1 (MQ=255)                                                                                                                                                                                                         cATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACgttcaggt                                <  1:376501/251‑1 (MQ=255)                                                                                                                                                                                                         cATGGTTCTGGTCAGGTGCAGAGGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACgttcaggt                                <  1:484155/251‑1 (MQ=255)                                                                                                                                                                                                                  ggTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGCCACTGGAAACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTcaggatccggttt                        >  2:323391/1‑249 (MQ=255)                                                                                                                                                                                                                  ggTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTaa                       <  1:197023/251‑1 (MQ=255)                                                                                                                                                                                                                        gTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCa                 <  1:637219/251‑1 (MQ=255)                                                                                                                                                                                                                               tGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAACGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCCTCCGTCTTGCGGGCGAAGGGGAAGAGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAACACCCGAtt          >  2:163833/1‑251 (MQ=255)                                                                                                                                                                                                                                  gtcAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATTCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTc       <  1:288963/249‑1 (MQ=255)                                                                                                                                                                                                                                  gtcAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTc       >  1:26773/3‑251 (MQ=255)                                                                                                                                                                                                                                      gggATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGc   >  1:573966/1‑251 (MQ=255)                                                                                                                                                                                                                                       ggATTCTTTGCCGTGCAGC‑ATGCCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCTATCTGTACGTTCAGGTTTAGGTTAAACAGCACCCGATTTTCGAGCg  <  2:538526/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 CGGCGATATTTTTGGCGGCGGACGTGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACTTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGCCAGGGATTCTTCGCTGTACAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCG  >  NC_000913/14461‑14940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑