| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,509 | A→G | A391A (GCA→GCG) | thrA → | fused aspartate kinase/homoserine dehydrogenase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,509 | 0 | A | G | 97.3% | 224.0 / ‑3.3 | 73 | A391A (GCA→GCG) | thrA | fused aspartate kinase/homoserine dehydrogenase 1 |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (35/36); total (36/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01 | |||||||||||
TTCCAATCTGAATAACATGGCAATGTTCAGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCG‑AAATTCTTTGCCGCACTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGA > NC_000913/1263‑1747 | ttCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTgg > 1:552754/1‑250 (MQ=255) tCCAATCTGAATAATATGGCAAGGTTCATCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTTCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGa < 1:394236/250‑1 (MQ=255) ccAATCTTAATAATATGTCAATGTGCAGCGTTTCCGGCCCGGGGATTAAAGGGATGGTTTGCATGGGGGCGCGCGTGATTTCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCACTCATCATCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGAAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTTGCGGTGAc < 2:594500/250‑1 (MQ=255) aaTCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGGGAC‑ggg > 1:340465/1‑250 (MQ=255) ataataTGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTg < 1:35666/251‑1 (MQ=255) ataataTGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTg < 2:266950/251‑1 (MQ=255) ataTGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGGATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGcc > 1:251315/3‑251 (MQ=255) taTGGCAATGTTCAGCGTTTCCGGCCCGGGGCTGCACGGGCTGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGCTGTCCCGCGCCCGTATTTCCGTGGTGCTGGTTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGGACTTAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACTGGAACgcttgccc > 1:254445/2‑244 (MQ=255) ggCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCAtt < 1:604702/250‑1 (MQ=255) cAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGGC‑GGAAAGGcggggcactttt > 2:237356/1‑240 (MQ=255) tGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGTTTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGGGTGCGAGCTGAACGGGCAATGAAGGAAGAGTTCTACCAGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGGGGC‑GGGACGGCTgggcacttatt > 2:570280/1‑240 (MQ=255) cAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAGATGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGGACGGCAGGCCATTATCTCgaggg > 1:24906/1‑247 (MQ=255) agcgtgtccggcccGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCggtggt < 2:302104/239‑1 (MQ=255) ccggcccGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGAt < 1:123992/246‑1 (MQ=255) ggcccGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGGCATTATTTCGGTGGG‑AGG‑GGATg‑g > 1:374563/4‑251 (MQ=255) cccGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGAGAC‑GGAACGGCTGGCCATTCACTCGGTGGT‑AGG‑GGATg‑gg > 1:198460/2‑249 (MQ=255) ccGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GTAt < 1:3999/251‑1 (MQ=255) ccGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GTAt < 1:323048/251‑1 (MQ=255) ccGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGGGAC‑TGGACGGCTGGCCCTTATCTCGGTGGT‑AGG‑GGATG‑GTAt > 1:319608/1‑251 (MQ=255) gAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTTTCTCGGGGGT‑AGG‑TGATG‑GTATGCGCACCt > 1:606164/1‑251 (MQ=255) gggtggCAGGGCGGCGCGCGTGGTTGCTGAGATATGACGCGTCCGTATTGCCGTGGTGCTGATTGCGCAATCATCTTCCGAATACAGTATCAGGTTCCGCGTTCCGCAAAGCGACTGGGTGCTAGCTGAACGGGCCATGCAGGAGGAGTTCTACTGGGAACTGAAAGAAGGCTTACTGGAGCCGCGGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GTATGCGCACCTTGCGTGGGa < 2:162740/247‑1 (MQ=255) ggCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GTATGCGCACCTTGCGTGGGAtct < 1:570280/250‑1 (MQ=255) ggCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCACGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GTATGCGCACCTTGCGTGGGAtctc < 1:343346/251‑1 (MQ=255) cggcgCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCG‑aaa < 1:196652/251‑1 (MQ=255) gcgTGTTTGCAGCGATGTCACGCGCTCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATGCAGTATCAGTTTCTGCGTTCCGCAAAGCGACCGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCG‑AAATTCttt < 1:643932/251‑1 (MQ=255) gtttGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGGACGGATGGCCATTAACACGG‑GGTGAGGGTG‑TT‑GTATGCGCACCATGGGTGGGATTTCGGCGCAAATT‑TTTGccc > 2:268561/2‑250 (MQ=255) tttGCAGCGATGTCACGCGCCCGTATGTCCTTGGTGGTGATTACGCAAGCGTCTACCGAATGCAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCGGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCG‑AAATTCTTTGCCg < 2:164807/250‑1 (MQ=255) aCGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑GGATG‑GTATGCGGAACTTGCGTGGGATCTCGGCG‑AAATTCTTTGCCGCGCTGGCCCGCGccc > 1:10537/1‑250 (MQ=255) gTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCG‑AAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAAc < 1:150850/251‑1 (MQ=255) ttCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GGATGCGCACCTTGCGTGGGATCTCGGCG‑AAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTg > 1:93488/1‑251 (MQ=255) ccGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑GGATGTGT‑TGCGCACCTTGCGTGGGATCTCGGCG‑AAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCACCATTGTc > 2:110947/1‑251 (MQ=255) gtggtgCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GTATGCGCAACTTGCGTGGGGTCTCGGCG‑AAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTTTC‑Gc > 1:655808/1‑251 (MQ=255) aCGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCG‑AAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAgg‑g < 1:8296/251‑1 (MQ=255) cGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCG‑AAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAgg‑g > 1:288402/1‑250 (MQ=255) cGCAATCATCTGCCGAATACGGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCG‑AAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑Ga < 2:340465/251‑1 (MQ=255) cAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGG‑AGG‑GGATG‑GTCAGGGCACCCTTCGGGGGATCTCGGCG‑AAAATCTTTGCCGCCCTGGCCCCCCCCA‑AGAACCACATTGTCCGCC‑TTGCTCAgg‑ggt > 2:290569/1‑248 (MQ=255) atcTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑GGATG‑GGATGCGCACCTTGCGTGGGATCTCGGCG‑AAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCCTTGCTCAGG‑GATCTTCTg > 1:400836/1‑251 (MQ=255) ctCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCG‑AAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCCACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAAc > 1:193722/2‑251 (MQ=255) tCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCG‑AAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGa < 1:352537/248‑1 (MQ=255) ccGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCG‑AAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACg < 1:274611/250‑1 (MQ=255) cagaatgcttgatctgTTTCTGCGTTCCGCAAAGCGACTGTGTGCGTGCTGAACGGGCACTGCAGTAAGTGTTCTTCCTGTATCTGACAGAATGCTTCCTGGTGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GTATGCGCACCGTGCGTGGGATCTCGGCG‑AAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATACCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGc < 2:514732/236‑1 (MQ=255) aataaagtaTCAGGTTCTGCGTTCGGCAAAGCGACGGTGTGCGAGCGGAACGGGCAATGCAGGAAGAGTTCTACCAGGAACTGAAAGAAGGCTTGCTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCG‑AAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCa < 2:251315/243‑1 (MQ=255) aaTACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGATCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGG‑AGG‑GGAGG‑GGAAGAGAACCCTGAGTGGGCTATCGGCG‑AAAATCTTTGCCGCGCTGGCCCGCGACA‑AAATAAACAATGTC‑GCACTTGCTCAGGAGA‑CTTCTGAAcccacc > 2:439439/1‑246 (MQ=255) tcagtaTCAGATTCTGCGTTCCGCAAAGCGACTGTGTGAGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACAGCTGGCAATTATCTCGGTGGT‑AGG‑CGGTA‑GTAAGCGCACCTTGCGTGGGATATCGAGG‑AAATTCTTTGCAGGTCTGGCCCGC‑CAACATTTCAACCTTTTC‑GCCCTAGCACAGG‑GATCTTCTTAACGcccaca > 2:510935/6‑245 (MQ=255) aCAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCG‑AAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAAtc < 1:237356/251‑1 (MQ=255) cagtatccgTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCG‑AAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GGTCTTCTGAACGCTCAAtct > 1:240711/9‑251 (MQ=255) agtaTCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGGGGG‑AGG‑TGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCG‑AAATTCTTTGCCGCGCTGGCCCGCGCCC‑ATATCCACATTGTC‑GCCATTGCTCCGG‑GATCTTCTGAACGCTCAAtctc > 1:375278/4‑251 (MQ=255) gtaTCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGACGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGGACGGCTGGCCATTATCTCGGTGGT‑AGG‑CGATG‑GTATGCGCACCTTTCGGGGGGTCCCCGCG‑GAAATCTTTGGCGCGCTGGCCCCCGCCA‑ATATCAACGTTTTC‑GCCCTTGCTCCGG‑GGTCTTCTggctgaccaatctct > 2:202015/3‑237 (MQ=255) tttCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑GGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCG‑AAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTgg > 1:420413/1‑251 (MQ=255) ttCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGACGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑CGATG‑GGATGCGCACACTGCGTAGGATCTCCGCG‑CAATTTTTTGCCCCGCAGGCCCGCGCCC‑ATATCAACATTTCC‑GCCCTTGCCCAGG‑GACCTTCTGAACGCACACTCTCCGTCGTggg > 2:253589/1‑250 (MQ=255) gCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCG‑AAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑Taaa < 1:202015/250‑1 (MQ=255) cgcaAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAATAGTTCTACCTGGAACTGAAAGAAGGCTTACGGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCG‑AAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACgat < 1:233340/249‑1 (MQ=255) cGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGGC‑GGAAAGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GGATGCGGACCTTGCGTGGGATCTCGGCG‑AAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTTTC‑GCCATTTCTCCGG‑GATCTTCTGAACGCTCAATATCTGTCGTGG‑TAAAAAACGATGATGCg > 1:356202/1‑250 (MQ=255) gACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCG‑AAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCATG‑GATCTTCTGAACGCTCAATCTCTGTCGTGGGTAAA‑AACGATGATGCGAc > 1:109989/1‑251 (MQ=255) gACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑GGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCG‑AAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGATGATGCGAc > 1:348264/1‑251 (MQ=255) aCTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCG‑AAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGATGATGCGAcc < 1:357840/251‑1 (MQ=255) ggCGAGCTGAACGGGCAATGCAGGAAGATTTCTACCTGGAACGGAAAGAAGTCTGACTGGAGCCGCGGGCAGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GTATGCGCACCTTGCGTGGGATCGCGGCG‑AAATTCTTTGCCGCACTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGATGATGCGACCACTGGc < 2:422321/250‑1 (MQ=255) gCGAGCTGAACGGGCAATGCAGGAAGAGTGCTACCTGGAACTGAAAGAAGGCTTACGGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCG‑AAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGAGGATGCGACCACTGGc < 2:288402/250‑1 (MQ=255) gAGCTGAACTGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGG‑AGG‑TGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCG‑AAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCTATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGATGATGCGACCACTGGCGTg < 1:96304/251‑1 (MQ=255) ggCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGGTG‑GTATGCGCACCGTGCGTGGGATCTCGGCG‑AAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCCTTGCTCAGG‑GATCTTCTGAACGCCCAATCTCTGTCGTGG‑TAAATAACGATGATGCGACCACTCGCGTGCGCGTTACtt > 1:616517/1‑250 (MQ=255) ggCAGGAAGAGTTCTACCTTGAACGGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCACTATCTCGGTGGT‑AGG‑TGATG‑GTATGCGCTCCTTGCGTGGGATCTCGGCG‑AAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGATGATGCGACCACTGGCGTGCGCGTTACtcatca < 2:10537/249‑1 (MQ=255) agTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑GGATG‑GGATGCGCACCTTGCGTGGGATCTCGGCG‑AAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTggtc > 1:510813/1‑248 (MQ=255) atcccTGGAACTGACAGAAGGCTTATTGGAGGCGGTGGTGGGGAC‑GGAACGGGTGGGCTTTATCTCGGTGGT‑AGG‑GGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCG‑AAAGTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACAGTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTTATCAGATGTTGTTCAAt < 2:606164/247‑1 (MQ=255) tACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGGGGT‑AGG‑GGGTG‑GTATGCGCAACTTGCGGGGGATCTCGGCG‑AAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GGTCTTTTGAACCCACCATCCCTGTCGGGG‑TAAATAACGCTAATGCGACCACTGGCGTGCGCGGTTCtcatccaggtcttgtcaatac > 2:156721/1‑235 (MQ=255) aCCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCGCGGTGGT‑AGG‑TGATG‑GTATTCGCACCTTGCGTGGGATCTCGGCG‑AAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATAc < 1:428889/250‑1 (MQ=255) gAACTGAAAGAAGGATTACTGGAGCCGCTGACAGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGAAG‑GTATGCGCACCCTGCGTGGGATCTCGGCG‑AAATTCTTTGCCGCACTGGCCCGCTCCA‑ATATCAACATTGTA‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGATGATGCGACCACTGGCGTGCGCGTTAATCATCAGATGCTGTTCAATACCGATc > 1:345450/1‑250 (MQ=255) agaagGCTTCTTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCG‑AAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCg < 2:93488/251‑1 (MQ=255) agaagGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCG‑AAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCg < 1:13668/251‑1 (MQ=255) agGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGG‑AGG‑GGATG‑GCATGCGCACCTTGGGTGGGATCTCGGCG‑AAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GGTCTTCTGGACGCCCAATCTCTGTCGTGG‑GAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGATGGTCAATACCGATAGGGTTATCGa > 1:289873/1‑249 (MQ=255) aCTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCG‑AAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGttt < 1:439439/250‑1 (MQ=255) aCTGGAGCCGCTGGCGGGGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑CGG‑TGCTG‑GGATGCGCCCCTTGCGTGGGATCTCGGCG‑AAATTCTTTGCCGCGCTGGCCCGCGCCC‑ATATCAACATTTTC‑GCCCTTGCTCAGG‑GTTCTTCTGAACGCCCACTCTCTGTCGGTG‑TAAATAACGCTGATGCGACCAATGGCGGGCGCGTTACTCATCAGGTGCTGTTCAATTCCGGTCCGGTTATCGGAGTttttt > 2:343543/1‑250 (MQ=255) gggtgCCGTTGGCGGTGAC‑GGAACGGCGGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GTATGCGCACCTTGCGTTGGATCTCGTCG‑AAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGAGCAGGTTATCGAAGTGTTtgtg < 2:109989/247‑1 (MQ=255) ggAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCG‑AAATTCTTTGCCGCGCTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGa < 1:87618/251‑1 (MQ=255) | TTCCAATCTGAATAACATGGCAATGTTCAGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCG‑AAATTCTTTGCCGCACTGGCCCGCGCCA‑ATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATCTCTGTCGTGG‑TAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGA > NC_000913/1263‑1747 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |