Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	14,647	T→C	T160T (ACT→ACC)	dnaJ →	chaperone protein DnaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	14,647	0	T	C	98.6%	210.0 / ‑6.0	69	T160T (ACT→ACC)	dnaJ	chaperone protein DnaJ
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (31/37);  total (32/37)
Fisher's exact test for biased strand distribution p-value = 4.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

GGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACTTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGCCAGGGATTCTTCGCTGTACAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGC  >  NC_000913/14414‑14883                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                              ggTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATgg                                                                                                                                                                                                                              >  1:477125/1‑251 (MQ=255)           gcggcgCAGCTTTCAGCTATATTTGTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAg                                                                                                                                                                                                                    <  2:360239/251‑1 (MQ=255)                gcAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGCCTCTGGAAGAGTGTG‑ACGTTTGCCACGGTATCGGTGCCAAACCCGGTACACACCAGCCACCCTGTCAGACCTGTCATGGTTATGGTCAggggc                                                                                                                                                                                                                >  2:514616/1‑247 (MQ=255)                 cAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGTCGTCAACGTGCGGCGCGCGGTGCTGCTTTACGCTATAACATGTAGCTCACCCTCGAAGAAGCTGTACGTGGAGTGACCAAAGAGATCCGCATTCCGACTATGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAACACCAGGTACACAGCCGCAGCCCTGTCCGACCTGTCATGTTTCTGGTCAGGGGCa                                                                                                                                                                                                               >  2:376501/1‑250 (MQ=255)                  aGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAACCCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAggggc                                                                                                                                                                                                                >  2:679438/1‑245 (MQ=255)                  aGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGc                                                                                                                                                                                                                >  1:465771/1‑248 (MQ=255)                     cTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGCAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGGTCTGGTCAGGTGCAGATgc                                                                                                                                                                                                          >  2:277735/1‑251 (MQ=255)                             atatTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGa                                                                                                                                                                                                  <  1:668959/251‑1 (MQ=255)                             atatTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGa                                                                                                                                                                                                  <  1:173501/251‑1 (MQ=255)                                tttttGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGAttc                                                                                                                                                                                               >  1:662648/1‑251 (MQ=255)                                  tttGGTGACGGTTTCGGCGATAGTGTTGGCGGCGGACGTGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTTGACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGAttct                                                                                                                                                                                              <  1:398192/250‑1 (MQ=255)                                   ttGGTGACGTTTTCGGCGATATTTTTGGCTGCGGACGTGGGCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCTCCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCATACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGAttctt                                                                                                                                                                                             <  2:663216/250‑1 (MQ=255)                                                cGGCGATATTTGTGGGGGCGGACGTGGTCGTCACCGTGCGGCGCGCGGTGCTGATTTCCGCTAGAACATGTAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGGCCAAAGAGAGCCGCATTCCGACTCTGGAAGAGTGTG‑ACGGTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGGCATGGTTCGGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTTcagc‑ag                                                                                                                                                                               <  2:445905/250‑1 (MQ=255)                                                   cGATATTTTTGGCGGCGGACGTGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCt                                                                                                                                                                           <  1:353713/251‑1 (MQ=255)                                                            tGGCGGCGGACGTGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCacac                                                                                                                                                                   <  1:89264/250‑1 (MQ=255)                                                                  gggACGTGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGTATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAgg                                                                                                                                                            <  1:206518/250‑1 (MQ=255)                                                                     gcGTGGGCGGCAACGGGGGGCGCGTGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGTCCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACTCTGTCAGGGcc                                                                                                                                                         <  2:477125/250‑1 (MQ=255)                                                                      cGTGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCcg                                                                                                                                                        <  1:277735/251‑1 (MQ=255)                                                                       gTGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCcgc                                                                                                                                                       >  1:43044/1‑251 (MQ=255)                                                                            cgtcAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTa                                                                                                                                                   <  1:379560/250‑1 (MQ=255)                                                                                aaCGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTg                                                                                                                                              >  1:137006/1‑251 (MQ=255)                                                                                        gcgcgcGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGa                                                                                                                                      <  1:119651/251‑1 (MQ=255)                                                                                           cgcgGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGCAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACACTGATCAACGATcc                                                                                                                                   >  2:229076/1‑251 (MQ=255)                                                                                             cgGTGCTGATTTACGCTATAAAATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCTTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCGGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGt                                                                                                                                 <  2:279058/251‑1 (MQ=255)                                                                                                 gCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGca                                                                                                                              <  1:437208/250‑1 (MQ=255)                                                                                                      tttACACTATAACCTGGAGCTAACCCTCGAAATCGCTGTCCGTGGCGCGACCACAGAGATCCGCATTCCGACTCTGGGAGAGTGTG‑ACGTTTGCCACGGCAGCGGTGCACAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGACGTGCAGCCAG‑CCTGTCAACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAAt                                                                                                                        >  2:668427/1‑251 (MQ=255)                                                                                                         aCGCTATAACATGGAGCTTACCCTCGAAGAAGCTGTACGTGGCGGTACCAAAGAGATCCGCATGCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATgt                                                                                                                      <  2:678971/250‑1 (MQ=255)                                                                                                          cGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATgtca                                                                                                                    <  1:439230/251‑1 (MQ=255)                                                                                                           gCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCTGTGCAACAAATgtcat                                                                                                                   <  1:514616/251‑1 (MQ=255)                                                                                                            cattaACATGGAGCTCATCCTCGCATAAGCGTTACGTGGCGTGAACAAATAGATCCGCATTCCGACCCTGTAAGTGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCTACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCAGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGGGCAACAAATgtcatg                                                                                                                  <  2:150789/248‑1 (MQ=255)                                                                                                                   aTGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGtcatggtcatgg                                                                                                           >  1:629722/1‑251 (MQ=255)                                                                                                                     ggAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGAGCCGCATTCCGACTCTGGAAGAGTGTG‑ACGGTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTGTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTcatggtcatggt                                                                                                          <  1:93515/250‑1 (MQ=255)                                                                                                                          tCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGTTCACAGAt                                                                                                                                     >  2:188067/1‑218 (MQ=255)                                                                                                                          tCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGAt                                                                                                                                     <  1:188067/218‑1 (MQ=255)                                                                                                                          ccACCCTCGAATAAGCTGTACGTGGCGTGACCAAAGAGAGCCGCATTCCGCATCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGTTGCAAAACCAGGTACACAGCCGCAGACCGGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCGGGGCCGCGGTACGCTGATCAAAGATCCGAGCAACAAATGGCATGGTCATGGTCGTGtt                                                                                                    <  2:22600/250‑1 (MQ=255)                                                                                                                             cccTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTc                                                                                                                                                                                                                           >  1:75083/1‑130 (MQ=255)                                                                                                                             cccTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTc                                                                                                                                                                                                                           <  2:75083/130‑1 (MQ=255)                                                                                                                                 cGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAgcg                                                                                               <  1:594888/249‑1 (MQ=255)                                                                                                                                 cGAAGAAGCTGGACGGGGCGTGACCAAAGAGATCCGCATTCCGACTCTTGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGAACTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTGGAGCgca                                                                                             <  2:286495/251‑1 (MQ=255)                                                                                                                                  gacgaagCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGAttctttgc                                                                                                                                                                                          >  1:566580/4‑155 (MQ=255)                                                                                                                                  gaagaaGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGAttctttgc                                                                                                                                                                                          <  2:566580/158‑4 (MQ=255)                                                                                                                                    agaagCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCgcagc                                                                                           >  1:94133/1‑250 (MQ=255)                                                                                                                                    agaagCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGAACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGAgcagg                                                                                           >  2:474798/1‑249 (MQ=255)                                                                                                                                             aCGTGGCGTGACCAAAGAGATCCGTATTCCGACTCTTGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTTTCAGGGCCGCGGTACGATGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCATCAAAACGCTGt                                                                                 <  2:393207/251‑1 (MQ=255)                                                                                                                                                 ggcggtaCCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTATCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCGGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGt                                                                             <  2:648939/245‑1 (MQ=255)                                                                                                                                                      gACCAAAGAGTTCCGCATTCCGACTCTGGAAGGGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTTGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTaaa                                                                         <  2:14059/250‑1 (MQ=255)                                                                                                                                                        ccAAAGAGATCCTCATTACGATTCTGGCAGAG‑GTGTACGTTTGCCTCGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGGCATGGGTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGGCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATc                                                                      <  2:449685/251‑1 (MQ=255)                                                                                                                                                               gaTCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGtt                                                                                                    >  2:594568/1‑214 (MQ=255)                                                                                                                                                               gaTCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGtt                                                                                                    <  1:594568/214‑1 (MQ=255)                                                                                                                                                                   cGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAggggg                                                           >  1:36208/1‑250 (MQ=255)                                                                                                                                                                       ttCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACCAATGTCATGGTCATGGTCGTGTTGAGCGAAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGac                                                       >  1:434790/1‑251 (MQ=255)                                                                                                                                                                       ttCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCTTGTTGAGCGCAGCAAAACGCGGTCCGTTAAAATCCCGGCAGGGGTGGac                                                       >  1:434745/1‑251 (MQ=255)                                                                                                                                                                         ccGACTCTGGAAGAGTGTG‑ACGTTTACCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTCAAATCCCGGCAGGGGTGGacac                                                     >  1:107125/1‑251 (MQ=255)                                                                                                                                                                            aCTCTGGAAGAGGGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTAAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGACGCGGTACGCTGATCAAAGATCCGTGCAACAACTGTCATGGGCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGGGGGCACTgg                                                  >  2:494426/1‑251 (MQ=255)                                                                                                                                                                                    agagTGTG‑ACGTTTGCCACGGAAGCGGAGCAAGACCAGGTACACAGCCGCAGACTTGGCCGACCTGTAATGGTTCTGGTCAGGTGCAGATGCGCCAGGGATTCTTCGCTGAACAGC‑AAACCTGTCCACACTGTCAGAGCCGCGGTACGCTGATCAAAGACCCGTGCAACAAATGTCATGCTCATGGTCGTGTTGCGCGCACAAAAACGCTATCAGGTAAAATCCCGGCGGGGGTGGACCCAGGCGACCGcc                                          >  2:351149/1‑250 (MQ=255)                                                                                                                                                                                       gtgtG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGGCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGTACACTGGAGACTGCATcc                                       <  2:94133/251‑1 (MQ=255)                                                                                                                                                                                         gtg‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGt                                     >  1:581874/1‑251 (MQ=255)                                                                                                                                                                                           g‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCt                                   >  1:367476/1‑251 (MQ=255)                                                                                                                                                                                             aCGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACCAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACCATGGGGACCGCATCCGTCtt                                  >  1:532170/1‑251 (MQ=255)                                                                                                                                                                                              cGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGACGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTg                                 >  1:549574/1‑251 (MQ=255)                                                                                                                                                                                                  tGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGAGGGAAACTGGAGACCGCATCCGTCTTTCggg                             >  2:642351/1‑251 (MQ=255)                                                                                                                                                                                                     cACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCg                           >  1:512015/1‑250 (MQ=255)                                                                                                                                                                                                        ggTAGCGGTGCAAAACAAGGCACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCAGCGGTACGCTGATCAACGATCCGTGCAACACATGTCATGGTCATGGTCGTGTTGAGCGCAGCTAAACGCTGTCCGTTAAAATCCCGGCAGGGGGGGACCCTGGAGAcgacatgcggattgaggtcgacgg                       >  2:310865/1‑228 (MQ=255)                                                                                                                                                                                                            gCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAAGCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGaa                   <  1:406188/251‑1 (MQ=255)                                                                                                                                                                                                                 gCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCggg              <  1:132665/251‑1 (MQ=255)                                                                                                                                                                                                                 gCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCgg               <  1:561286/250‑1 (MQ=255)                                                                                                                                                                                                                     aaCCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTACTGGGCAGGTGCAGATGCGTCAGGGATTCGTTGCCGTGCAGC‑AGACCGGTCCACACTGTCAGGGCCGCGGTACGCTGAACAAAGATCCCTGCAACAAATGTCATGGTCATGGGCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTCGACACTGGAGACCGCATCCGTCTTGCGGGc                            <  1:257726/233‑1 (MQ=255)                                                                                                                                                                                                                            gacacAGCCGCAGACCTGGCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGgcg   <  1:429653/250‑1 (MQ=255)                                                                                                                                                                                                                             acacAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGAGCAGATGCGTCAGGGATTCTTTGCCGTGCAGC‑AACCCTGTCCACACTGTCAGAGCCGCGGTACGCTGATAAAAGATCCGTGCAACAAATGTCATGGACATGGTCATGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATACCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGGAGGAGAAGCGGGCGTGCATGgcgc  >  1:630821/1‑251 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                              GGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTG‑ACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACTTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGCCAGGGATTCTTCGCTGTACAGC‑AGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGC  >  NC_000913/14414‑14883

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑