Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	13,029	C→T	D289D (GAC→GAT)	dnaK →	chaperone protein DnaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	13,029	0	C	T	94.3%	193.6 / 2.2	71	D289D (GAC→GAT)	dnaK	chaperone protein DnaK
Reads supporting (aligned to +/- strand):  ref base C (2/2);  new base T (39/27);  total (42/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.33e-01

AAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGAT‑CAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATCGACGACGTTATCCTCGTTGGTGGTCAGACTCGTATGCCAATGGTTCAGAAGAAAGTTGCTGAGTTCTTTGGTAAAGAGCCGCGTAAAGACGTTAACCCGG  >  NC_000913/12788‑13264                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                            aaGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCCACTACCTGGTTGAAGAATTCAAGAAAGAT‑CAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGt                                                                                                                                                                                                                                    >  1:639562/1‑251 (MQ=255)     tGACGGCGAAAAAACCTTCGAAGTGCTGGCAACCAACGGTGATACCCTCCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGAT‑CAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCg                                                                                                                                                                                                                                 <  2:217711/250‑1 (MQ=255)       ccctacacgacactctcccgactttGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGAAAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGAT‑CAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGTAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGaaa                                                                                                                                                                                                                              <  2:369292/227‑1 (MQ=255)                aaaCCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGAT‑CAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAAc                                                                                                                                                                                                                     >  1:667448/1‑251 (MQ=255)                 aaCCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGAT‑CAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACa                                                                                                                                                                                                                    <  1:456975/251‑1 (MQ=255)                  aCCTTAGAAGTTCTGGCAACCAAAGGTAACACCAAACTGGGTGGTGACGACTCCGACCGCAGTCAGATCAACTACCTGGTTGAAGAATTCAAGAAAGCT‑CAGGGCATTGACCTGCGCAACGATACGCTGGCAATGCAGCGCCTGCAAGAAGAAGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGCCCGCAGTTAACCAGCCGTCAATCACTGCAGATGCGACCGCCACGAAACACATGaaat                                                                                                                                                                                                                    >  2:659860/1‑248 (MQ=255)                        gAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGAT‑CAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGt                                                                                                                                                                                                             >  1:294601/1‑251 (MQ=255)                               tGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGAT‑CAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGaaa                                                                                                                                                                                                                              >  1:369292/1‑227 (MQ=255)                                   aaccaacGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGAT‑CAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCg                                                                                                                                                                                                   >  1:326817/1‑250 (MQ=255)                                      caacGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGAT‑CAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAAc                                                                                                                                                                                               <  1:286867/251‑1 (MQ=255)                                          ggTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGAT‑AAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCCGAAAAAGCGAAAATCGACCTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGaaccggg                                                                                                                                                                                            >  2:306015/1‑245 (MQ=255)                                           cctaTACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGACCAACTATCTGGTTGAAGAATTCAAGAAAGAT‑CAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAA‑GCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGaaa                                                                                                                                                                                         <  1:342057/248‑1 (MQ=255)                                            ggATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGAT‑CAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGaaa                                                                                                                                                                                         <  1:567715/250‑1 (MQ=255)                                             gATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGACAGAT‑CAGGGCATTGACCTGCGCAACGATACGCTGGAAAAGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGACAATCGCACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACAAATGAACATCAAAGTGACTCGTGCGAAACTGGaaa                                                                                                                                                                                         >  2:399771/1‑250 (MQ=255)                                              aTACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGCAAGAT‑CAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAA‑GCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGaaa                                                                                                                                                                                         >  2:342057/1‑248 (MQ=255)                                                aCCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGAT‑CAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAACATCGAACTGTCTTCCGCTCAGCAGACAGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGCAACACATAAACATCAAAGAGACTCGTGCGCAACTGGACAGCCt                                                                                                                                                                                     >  2:284925/1‑251 (MQ=255)                                                 cccACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGAT‑CAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTg                                                                                                                                                                                    <  1:49258/251‑1 (MQ=255)                                                   cACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGAT‑CAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGt                                                                                                                                                                                  >  1:427149/1‑251 (MQ=255)                                                    cccTGGGTGGGGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAACTCAAGAAAGAT‑CAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGtt                                                                                                                                                                                 <  1:481796/250‑1 (MQ=255)                                                    aCCTGGGTGGTGCAGACTTCGACAGCCGTCTAATCAACTACCTGGTTGAAGCATTCAAGAAAGAT‑CAGGGCATTGACCTGCGCAACGAACCGCTGGCAATGCAGCGCCTGCAAGAAGCGGCAGAACAAGCGAAAATCGAACTGTCTTCCGCTCCGCAGGCCGCCGTTACCCTTCCGTACATCACTGCCGATGCGACCGGTCCGAAACAAATGACAATCAAAGTGACTCGTGCGAAACAGGAAAGCCTGGtt                                                                                                                                                                                 >  2:673158/1‑251 (MQ=255)                                                     ccTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGAT‑CAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTg                                                                                                                                                                                <  1:429219/251‑1 (MQ=255)                                                          gtggTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGAT‑CAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGa                                                                                                                                                                               >  1:214669/3‑247 (MQ=255)                                                          gtggTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGAT‑CAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGAt                                                                                                                                                                           <  1:35365/249‑1 (MQ=255)                                                            ggTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGAT‑CAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTACGAAACACATTAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTAGAAGATCt                                                                                                                                                                         >  2:324918/1‑251 (MQ=255)                                                                  gACTTCGCCAGCCGTCTGATCAACTACCTGGTTGAAGAATTCACGACCGAT‑CAGGGCATTGACCTGCGCAACGATCAGCTGGCAATGCAGCGCCTGAAAGAAGCGGAAGAAAAAGCGAAAATAGAACTGTCTTCCGCTCAGCAGACCGCCGTTAACCTGCCGTACATCACTGCAGCAGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGAGAAACTGGAAAGCCTGGTTGAAGATCTggaaa                                                                                                                                                                    >  2:435838/1‑247 (MQ=255)                                                                      tCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGAT‑CAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCg                                                                                                                                                                <  1:399771/250‑1 (MQ=255)                                                                             ccGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGAT‑CAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGAGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTg                                                                                                                                                        >  1:487153/1‑251 (MQ=255)                                                                                tCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGAT‑CAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCGGGTTGAAGATCTGGTAAACCGTTCCATTgcgc                                                                                                                                                     >  2:225634/1‑248 (MQ=255)                                                                                         ctcccTGGTTGAAGAATTCAAGAAAGAT‑CAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGTCGTTAACCTGCCGTACATCACTGCAGATGCGACAGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGACACTGGAAAGACTGGTGGAAGATCTGGTAAAccataccagtgcaccgctgaagg                                                                                                                                            >  2:142895/5‑230 (MQ=255)                                                                                           accTGGTTGAAGAATTCAAGAAAGATACAGG‑CATGGACCTGCGCAACGATCCGCTGGCCATGCAGCGCCAGAAAGAAGCGGCAGAAAAAGCGAAAAGCGAACTGTCTACCGCTCAGCAGACCGCCGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGtt                                                                                                                                          <  2:462373/249‑1 (MQ=255)                                                                                                       aaTTCAAGAAAGAT‑CAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGAc                                                                                                                              <  1:442594/251‑1 (MQ=255)                                                                                                        aTTCAAGAAAGAT‑AAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAACCACATGAACATCAAAGTGACTCGAGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTCCCATTGAGCCGCTGAAAGTTGCAcagcagtac                                                                                                                              >  1:493258/1‑247 (MQ=255)                                                                                                          tCAAGAAAGAT‑CAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCt                                                                                                                           >  1:211645/1‑251 (MQ=255)                                                                                                                       aGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGt                                                                                                                >  1:495653/1‑250 (MQ=255)                                                                                                                            aTTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTg                                                                                                           >  1:112828/1‑250 (MQ=255)                                                                                                                            aTTGACCTGCGCAACGATCCGATGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGATCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTAGAAAGCCTGGTTTAAGATCTGGTAAACCGTTCCATTGATCCGCTGAAAGTTGCACTGCAGGAAGCTGGCCttcccggatctgt                                                                                                          >  2:86269/1‑239 (MQ=255)                                                                                                                               gACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATAAAAGTGACTCGTGCGAACCTGGAACGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCGGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGatat                                                                                                       >  2:242462/1‑251 (MQ=255)                                                                                                                                 ccTGCGCAACCATCCACTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAACAGCGAAAATCGAACTGTCTTCAGCACAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAAAATCAAAGTGACTCGTGCGAACCTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGACAGTTGCACTGCAGGACGCAGGCCTGTACGTATCTGATATcg                                                                                                     >  1:272213/1‑251 (MQ=255)                                                                                                                                    gcgcAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATcga                                                                                                    <  1:27867/249‑1 (MQ=255)                                                                                                                                    gcgcAACGATCCGCGGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATcgac                                                                                                   <  2:571416/250‑1 (MQ=255)                                                                                                                                     cgcAACGACCCGCTGGCAATGCAGCGCCTGAACGAAGCGGCAGAACAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAACCTGGAAAACCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCCCTGCAGGACGCTGGCCTGTCCGTATCTGATATcgacgc                                                                                                 >  2:44759/1‑250 (MQ=255)                                                                                                                                       caactacccGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTTACTCGTGCGAAACTGGAAAGTCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATCGacgacg                                                                                               <  1:174271/244‑1 (MQ=255)                                                                                                                                       cAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGACAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATcgacgccg                                                                                               >  2:83960/1‑248 (MQ=255)                                                                                                                                          cGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATCGACGACGTTa                                                                                            <  1:284925/251‑1 (MQ=255)                                                                                                                                                  tGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATCGACGACGTTATCCTCGtt                                                                                    >  1:23761/1‑251 (MQ=255)                                                                                                                                                    caaagtccgggtcTGAAAGAAGCGGCACAAAAAGCGAAATGCTAACTGTCTTCCGCTCAACAGACCGACGTTAACCTGCCGTACATCACTGCATATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCTTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCTCGGAAAGTTGCACGGCAGGACGCTGGCCTGTCCGTATCTGATATCGACGACGTTATCCTCGTtgg                                                                                  <  2:426652/239‑1 (MQ=255)                                                                                                                                                     cAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATCGACGACGTTATCCTCGTtggg                                                                                 >  1:96310/1‑250 (MQ=255)                                                                                                                                                           aGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATCGACGACGTTATCCTCGTTGGTGGTc                                                                             >  2:601551/1‑249 (MQ=255)                                                                                                                                                                   aaaGAAGCGGCATAAAAAGCGAAAAACGAACTGTCATCGGCTCAGAAGTCCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATCGACGACGTTATCCTCGTTGGTGGTCAGACTCGTAt                                                                   <  2:23761/251‑1 (MQ=255)                                                                                                                                                                     agaagCGGCAGAAAAAGCGAAAATCGCACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGAACATCAATGCAGATGCGTCCGGTCCGAAACACAGGACCATCACAGTGACTCGTGCGAAAATGGAAAGCCGGGGTGAAGATATGGTAAACAGTTCCATTAAGCCGCTGAAATTCGCACTGCAGGACGCGAGCCTGTCTGTATCTGATATCTCCAACGTTATCCTCGTtggtggactgagtcgcgtgc                                                                 >  2:7427/1‑237 (MQ=255)                                                                                                                                                                         gCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATCGACGACGTTATCCTCGTTGGTGGTCAGACTCGTATGCCAAt                                                             <  1:306015/251‑1 (MQ=255)                                                                                                                                                                          cGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATCGACGACGTTATCCTCGTTGGTGGTCAGACTAGTATGCCAATg                                                            >  1:106088/1‑251 (MQ=255)                                                                                                                                                                                      cGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATCGACGACGTTATCCTCGTTGGTGGTCAGACTCGTATGCCAATGGTTCAGAAGaaa                                                <  1:601551/251‑1 (MQ=255)                                                                                                                                                                                           aTCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATCGACGACGTTATCCTCGTTGGTGGTCAGACTCGTATGCCAATGGTTCAGAAGAAAGTTGc                                           <  1:4341/251‑1 (MQ=255)                                                                                                                                                                                              gAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATCGACGACGTTATCCTCGTTGGTGGTCAGACTCGTATGCCAATGGTTCAGAAGAAAGTTGCTg                                         >  1:85554/1‑250 (MQ=255)                                                                                                                                                                                              gAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAAATGGAAAGCCTGGTTGAAGATCTGGTAA‑CCGTTCCATTGAGCCGCTGCAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATCACCGACGTTATCCTCGTTGGTGGTCCGCCTCGGATGCCAAGGGTTCGGAAGAAAGTTGCTg                                         >  2:573809/1‑249 (MQ=255)                                                                                                                                                                                                 cTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCa                                                                                                                                       <  1:552430/153‑1 (MQ=255)                                                                                                                                                                                                 cTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCa                                                                                                                                       >  2:552430/1‑153 (MQ=255)                                                                                                                                                                                                    gcTTCCGCACAGCGGAACGACGTTAACCTGCCGTACATCACTGCGGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATCGACGACGTTATCCTCGTTGGTGGTCAGACTCGTATGCCAATGGTTCAGAAGAAAGTTGCTGAGTTCt                                   <  2:326817/249‑1 (MQ=255)                                                                                                                                                                                                     cTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATCGACGACGTTATCCTCGTTGGTGGTCAGACTCGTATGCCAATGGTTCAGAAGAAAGTTGCTGAGTTCttt                                 >  1:120/1‑251 (MQ=255)                                                                                                                                                                                                        ccGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATCGACGACGTTATCCTCGTTGGTGGTCAGACTCGTATGCCAATGGTTCAGAAGAAAGTTGCTGAGTTCTTTgg                               <  1:659627/250‑1 (MQ=255)                                                                                                                                                                                                           cTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATCGACGACGTTATCCTCGTTGGTGGTCAGACTCGTATGCCAATGGTTCagaa                                                    >  1:260057/1‑226 (MQ=255)                                                                                                                                                                                                             cagcagACCGACGTGAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATCGACGACGTTATCCTCGTTGGTGGTCAGACTCGTATGCCAATGGTTCAGAAGAAAGTTGCTGAGTTCTTTGGTAAaga                         <  1:312103/251‑1 (MQ=255)                                                                                                                                                                                                                 agcccGACGTTAACCTGCCGTACAGCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATCGACGACGTTATCCTCGTTGGTGGTCAGACTCGTATGCCAATGGTTCAGAAGAAAGt                                              <  2:551258/223‑1 (MQ=255)                                                                                                                                                                                                                 agACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATCGACGACGTTATCCTCGTTGGTGGTCAGACTCGTATGCCAATGGTTCAGAAGAAAGt                                              >  1:551258/1‑226 (MQ=255)                                                                                                                                                                                                                     cGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATCGACGACGTTATCCTCGTTGGTGGTCAGACTCGTATGCCAATGGTTCAGAAGAAAGTTGCTGAGTTCTTTGGTAAAGAGCCGCGt                  >  2:544028/1‑250 (MQ=255)                                                                                                                                                                                                                      gACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTg                                                                                                                     <  1:38453/150‑1 (MQ=255)                                                                                                                                                                                                                      gACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTg                                                                                                                     >  2:38453/1‑150 (MQ=255)                                                                                                                                                                                                                         gTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATCGACGACGTTATCCTCGTTGGTGGTCAGACTCGTATGCCAATGGTTCAGAAGAAAGTTGCTGAGTTCTTTGGTAAAGAGCCGCGTaa                >  1:558954/1‑248 (MQ=255)                                                                                                                                                                                                                                 cccgtACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATCGACGACGTTATCCTCGTTGGTGGTCAGACTCGTATGCCAATGGTTCAGAAGAAAGTTGCTGAGTTCTTTGGTAAAGAGCCGCGTAAAGACGTTAAcc     <  2:457894/247‑1 (MQ=255)                                                                                                                                                                                                                                    gtACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATCGACGACGTTATCCTCGTTGGTGGTCAGACTCGTATGCCAATGGTTCAGAAGAAAGTTGCTGAGTTCTTTGGTAAAGAGCCGAGTACAGACGTTAACCCgg  >  1:234517/2‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                            AAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGAT‑CAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATCGACGACGTTATCCTCGTTGGTGGTCAGACTCGTATGCCAATGGTTCAGAAGAAAGTTGCTGAGTTCTTTGGTAAAGAGCCGCGTAAAGACGTTAACCCGG  >  NC_000913/12788‑13264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑