Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	12,846	G→T	G228G (GGG→GGT)	dnaK →	chaperone protein DnaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	12,846	0	G	T	92.2%	157.5 / 6.7	67	G228G (GGG→GGT)	dnaK	chaperone protein DnaK
Reads supporting (aligned to +/- strand):  ref base G (3/2);  new base T (31/28);  total (36/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.45e-01

CGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGCACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTG  >  NC_000913/12613‑13090                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                     cGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTAACATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAg                                                                                                                                                                                                                                     <  2:646815/251‑1 (MQ=255)    cAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCTACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCCCCTGGGTGGTGAAGCCTTCGACAGcc                                                                                                                                                                                                                                   <  2:88220/250‑1 (MQ=255)    cAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGcc                                                                                                                                                                                                                                   <  1:359242/250‑1 (MQ=255)     aGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGt                                                                                                                                                                                                                                 >  1:217711/1‑251 (MQ=255)           ccAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGCACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATc                                                                                                                                                                                                                           >  2:56840/1‑251 (MQ=255)            cAAAGACGCCGGCCGTCTCGCTGGTCTGGCAGTTAAATGTATCCTCAACTAACCGATCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGATTCTGGCAACCGTACGATCGCTGTTTATGTCCTGGGTGGTGGTACTTTCCATATTTCTATTATCGAAATCGACGAAGTCTACGGCGAAAATACCGTCGAACTTCTGGCAACCAACGGTGATCCCCACCTTGGTGGTGAATACTTCGACAGCCTTCTGATCa                                                                                                                                                                                                                          <  2:542179/251‑1 (MQ=255)                             ccGCTGGTCGGGAATTAAAACGTAACATCAACGAACCGACCGCAGCTGCGCTGGTTTACGGTCTGGAAAAAGGTACTGGCAACCGGACTATCGCGGTTTATGCCCTGGGTGGTGGTACTTTCTATATTTCTATTATCTAAATCGACGAAGTTGACGGCGAAAAAACCTTCGACGTTCTGTCAACCAACGGTGATACCCACCTGGTTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTtaagaa                                                                                                                                                                                                         <  2:449273/250‑1 (MQ=255)                              cacgtgtctagaacgaaaacggttcatcaTCGAACCGACCGCAGCTGCGCTGTCTTACTGTCTGTAAACATGTACGTGCAACCTTACCATCGCGGTTTAGGACCTGGGTTGGGGTACATACGATATTTCTATTACCGAAATCGACGAAGTAGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGTTGACATCCCCCTGGGTGGTGAATACGTCGACAGTCGGCTTAGCAACTTCCTGGTTgaagaa                                                                                                                                                                                                         <  2:330456/227‑1 (MQ=255)                                  ggTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCa                                                                                                                                                                                                     >  1:358995/1‑250 (MQ=255)                                   gTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTCTCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAACCCTTAGAAGTTCTGGCAACCAACGGAGATACCCACCTGGGAGGTGAAGACTTCGACAACCGTCTGATCACCTACATGGTTGACGAAttaa                                                                                                                                                                                                     >  2:442594/1‑247 (MQ=255)                                      tGGAAGTAAAACGTATCATCCACGAACCGACCGCAGCTGCGCTGGCATAAGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGCTATTTCTATTATCGAAATCGACGACGATGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGATGATACCAACCTGGGTGGTGAAAACTCCGACAGCCGGCTGATAAACAACCTGATTGAAGAATGCaagaa                                                                                                                                                                                                 >  2:481796/1‑250 (MQ=255)                                                   tATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATAGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGCAGTTCTGGAAACAAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATAAACTACCAGGTGGAAGGATTAAAGAAAGATCAAGGCATTg                                                                                                                                                                                   >  2:456975/1‑251 (MQ=255)                                                      catcaACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGAcc                                                                                                                                                                                <  2:111663/251‑1 (MQ=255)                                                       agcaACGAACCGACCGCAGCTGCGGTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTAGCGCGGTTTATGACCTGGGTGGTGGTACTTTAGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCGGGTTGAATAATTCAAGAAAGATCAGGGCATTGACCt                                                                                                                                                                               <  2:322370/249‑1 (MQ=255)                                                          aaCGAACCGTCCTCAGCTGCGCTGGTTTACGGTCTGGCCAAAGGTACTTGCAACCGTACTATCGCGGTTTATGGCCTGGGTGGTGGTATTTACGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACGTCGACAGCCTTCTGAGCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGTTCATTGGCCTgcg                                                                                                                                                                            <  2:652953/251‑1 (MQ=255)                                                            cGAACCGACCGCAGCTGCGCTGGCTTACGGGCTGGACAAAAGTACTGGCACCCGGACTCTCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGGCGGCGAAAAACCCTTCGAAGTTCTGGCACCCCACGGTGATACCCACCTGGGTGGGGACGACTTCGACCGCCATCTGATCCACTACCTGGTTGAAGTATTCaggaagtataagggacatgacctgcgc                                                                                                                                                                           >  2:174271/1‑228 (MQ=255)                                                             gAACCGACCGCAGCCGCGCGGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTCCGACAGCCGTCTGATCAACTACCTGGTTGAAGAAt                                                                                                                                                                                                        <  1:101373/220‑1 (MQ=255)                                                               accgaccgCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACg                                                                                                                                                                       >  1:170583/1‑251 (MQ=255)                                                                             cgcTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATg                                                                                                                                                         >  1:457894/1‑251 (MQ=255)                                                                              gcTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGAGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGc                                                                                                                                                        >  1:426652/1‑251 (MQ=255)                                                                                 ggcgtgcGGTCTGGACAATGGTACTGGCAAACGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTTACGGCTAAAAAACCTTCGAAGGTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCACCTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAgc                                                                                                                                                     <  2:678034/245‑1 (MQ=255)                                                                                 ggCTTACGGTCTGGACAAAGGCACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAg                                                                                                                                                      <  1:56840/250‑1 (MQ=255)                                                                                   cTTACGGTCTGGACAAAGGTACTGGCAAACGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTAACGACATCGACGAAGTTGGCGGCGAAAAAACCTTCGAAGTTCTGGCCACCAACGGTGATACACACCTGGGTGGTGAAGACTTAGACCGCCGTCTGATCAGATACCTGGTTGTAGAATGCAAGGAAGGTCAAGACATTGACCTGAGCAACGacccactgaccaagcaactc                                                                                                                                                   >  2:312103/1‑232 (MQ=255)                                                                                               aCAAAGGTACTGGCAACCGTAATATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGATTTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCg                                                                                                                                       >  1:101112/1‑251 (MQ=255)                                                                                                  aaGGTACTGGCAACCGTACTATCGCGGTTTATGCCCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCa                                                                                                                                    <  1:28539/251‑1 (MQ=255)                                                                                                           gCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCg                                                                                                                           <  1:650643/251‑1 (MQ=255)                                                                                                            cAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGAACAAGGCATTGACATGCGCAACGAACAGCTGGCATTGCATCGCCTGACAGAAGCGGCAGaaaaatcg                                                                                                                           >  2:283169/1‑247 (MQ=255)                                                                                                             aaCCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGaa                                                                                                                         <  1:314256/251‑1 (MQ=255)                                                                                                               ccGTACTATCGCGGTTTATGACCTGGGGGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTCCCTGGCTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCACCGCCTGAAAGAAGCGGCAGAAAAAGCGaaa                                                                                                                        >  2:91002/1‑250 (MQ=255)                                                                                                                      aTCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGTTGATACCCACCTGGGTGTTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAAc                                                                                                                 <  2:626074/250‑1 (MQ=255)                                                                                                                      aTCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAAc                                                                                                                 <  1:280995/250‑1 (MQ=255)                                                                                                                           ggTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCtt                                                                                                           <  2:170583/251‑1 (MQ=255)                                                                                                                                   aCCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGACAAAACCTTCGAAGTTCTGGCAACCAACGGCGATACCCACCTGGGTGGTGAAGACTTCGACAGCAGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAACAGCGAAAATCGAACTGTCTTCCGCTc                                                                                                     >  1:641777/1‑249 (MQ=255)                                                                                                                                             tggtACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAAAGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACg                                                                                         >  2:429219/1‑251 (MQ=255)                                                                                                                                              tgtACTTTCGATATTTCTATTATCAAAATCGACGACGTTGCCGGCGAAAAAACCTTCGAAGTTCTGGCACCCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGACAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCCGACAAAGCGAAAATCGAACTATCTTCCCCCCAGCcgaccgacct                                                                                        >  2:344765/2‑249 (MQ=255)                                                                                                                                                 actatCGAGATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAATGCCCTCGAAGTTCTGACAACCAACGGTGATACCCACCTGGGCGGTGAAGACTTCGACAGCCTTCTGACCAACTACCTGGTTGAATAATTCAAGAAATATCAGTTAATTGACCGGCGGAACAATCCGCGCGCAATGCAGCGCCCCTAAGTCGCGGCACAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTaa                                                                                     <  1:403627/247‑1 (MQ=255)                                                                                                                                                   tttCGATCTTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGACGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGCAGACTTCGACAGCCGTCTGATCAACTACATGGTTGAAGCATTCAAGAAAGCTCAGGGCATTGACCTGCGCAACGATCCGATGGCAATGCAGCGCCTTAAAGAAGCGGCAGAAAAAGCGAAAATCGAAATGGCTTCCGACCAGCAACCCGACGtacaac                                                                                   >  2:8678/1‑246 (MQ=255)                                                                                                                                                    ttCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCt                                                                                  >  2:567715/1‑251 (MQ=255)                                                                                                                                                      cGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCAACCTGGGAGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTAAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCCAAAATCGAACTGTCTTCCGCTCCGCAGACCGACGTTAAccggc                                                                                >  2:44820/1‑248 (MQ=255)                                                                                                                                                               tattatCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAATGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATc                                                                        >  1:211237/1‑250 (MQ=255)                                                                                                                                                                 ttatCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCATCTACCTGGTTGAAGAATTCAAGAAAGATCAGGGTATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAg                                                                                                                                   <  1:680384/189‑1 (MQ=255)                                                                                                                                                                 ttatCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACt                                                                     >  1:571416/1‑251 (MQ=255)                                                                                                                                                                  taacGAAATCGACGAAGGTGACGGCGAAAAAACCGACGAAGTTCTGGCAACCAACTGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACt                                                                     <  2:130886/247‑1 (MQ=255)                                                                                                                                                                   atCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGc                                                                   >  2:286867/1‑251 (MQ=255)                                                                                                                                                                                aaGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCCACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGt                                                      >  1:639562/1‑251 (MQ=255)                                                                                                                                                                                    tGACGGCGAAAAAACCTTCGAAGTGCTGGCAACCAACGGTGATACCCTCCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCg                                                   <  2:217711/250‑1 (MQ=255)                                                                                                                                                                                      ccctacacgacactctcccgactttGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGAAAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGTAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGaaa                                                <  2:369292/227‑1 (MQ=255)                                                                                                                                                                                        ggCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGAt                                                                                                                                                                     >  1:551996/1‑132 (MQ=255)                                                                                                                                                                                        ggCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGAt                                                                                                                                                                     <  2:551996/132‑1 (MQ=255)                                                                                                                                                                                               aaaCCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAAc                                       >  1:667448/1‑251 (MQ=255)                                                                                                                                                                                                aaCCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACa                                      <  1:456975/251‑1 (MQ=255)                                                                                                                                                                                                 aCCTTAGAAGTTCTGGCAACCAAAGGTAACACCAAACTGGGTGGTGACGACTCCGACCGCAGTCAGATCAACTACCTGGTTGAAGAATTCAAGAAAGCTCAGGGCATTGACCTGCGCAACGATACGCTGGCAATGCAGCGCCTGCAAGAAGAAGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGCCCGCAGTTAACCAGCCGTCAATCACTGCAGATGCGACCGCCACGAAACACATGaaat                                      >  2:659860/1‑248 (MQ=255)                                                                                                                                                                                                       gAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGt                               >  1:294601/1‑251 (MQ=255)                                                                                                                                                                                                              tGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGaaa                                                >  1:369292/1‑227 (MQ=255)                                                                                                                                                                                                                  aaccaacGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCg                     >  1:326817/1‑250 (MQ=255)                                                                                                                                                                                                                     caacGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAAc                 <  1:286867/251‑1 (MQ=255)                                                                                                                                                                                                                         ggTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATAAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCCGAAAAAGCGAAAATCGACCTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGaaccggg              >  2:306015/1‑245 (MQ=255)                                                                                                                                                                                                                          cctaTACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGACCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAA‑GCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGaaa           <  1:342057/248‑1 (MQ=255)                                                                                                                                                                                                                           ggATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGaaa           <  1:567715/250‑1 (MQ=255)                                                                                                                                                                                                                            gATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGACAGATCAGGGCATTGACCTGCGCAACGATACGCTGGAAAAGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGACAATCGCACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACAAATGAACATCAAAGTGACTCGTGCGAAACTGGaaa           >  2:399771/1‑250 (MQ=255)                                                                                                                                                                                                                             aTACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGCAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAA‑GCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGaaa           >  2:342057/1‑248 (MQ=255)                                                                                                                                                                                                                               aCCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAACATCGAACTGTCTTCCGCTCAGCAGACAGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGCAACACATAAACATCAAAGAGACTCGTGCGCAACTGGACAGCCt       >  2:284925/1‑251 (MQ=255)                                                                                                                                                                                                                                cccACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTg      <  1:49258/251‑1 (MQ=255)                                                                                                                                                                                                                                  cACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGt    >  1:427149/1‑251 (MQ=255)                                                                                                                                                                                                                                   cccTGGGTGGGGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAACTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGtt   <  1:481796/250‑1 (MQ=255)                                                                                                                                                                                                                                   aCCTGGGTGGTGCAGACTTCGACAGCCGTCTAATCAACTACCTGGTTGAAGCATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGAACCGCTGGCAATGCAGCGCCTGCAAGAAGCGGCAGAACAAGCGAAAATCGAACTGTCTTCCGCTCCGCAGGCCGCCGTTACCCTTCCGTACATCACTGCCGATGCGACCGGTCCGAAACAAATGACAATCAAAGTGACTCGTGCGAAACAGGAAAGCCTGGtt   >  2:673158/1‑251 (MQ=255)                                                                                                                                                                                                                                    ccTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTg  <  1:429219/251‑1 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                     CGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGCACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTG  >  NC_000913/12613‑13090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑