| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,407 | C→T | S357S (AGC→AGT) | thrA → | fused aspartate kinase/homoserine dehydrogenase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,407 | 0 | C | T | 97.1% | 209.1 / NA | 71 | S357S (AGC→AGT) | thrA | fused aspartate kinase/homoserine dehydrogenase 1 |
| Reads supporting (aligned to +/- strand): ref base C (1/0); major base T (37/31); minor base G (1/1); total (39/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAACATGGCAATGTTCAGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCG‑AAATTCTTTGCCGCACTGGCCCGCGCCAATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATC > NC_000913/1169‑1653 | tCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAACATGGCAATGTTCAGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATTCAGCATCAGTTTCTGc > 1:422321/1‑251 (MQ=255) cTTGCCTGATTAAAAATACCGGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGGGTGTTTGCAGGGATGTCACGGGCCCGTATTTCCGGGGGGGTGATTTAGAGATAATCTTCCGAATACAGTATCAGTTTCTGCggt > 2:268555/1‑249 (MQ=255) gCCTGATTAAAAATACCGGAAATCCTCAAGCTCCAGGGACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCACGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTTAAGCGATGTCACGCGCCCGTATTTCAGTGGTGCTGATTACACAATCATCTTCCTAAACCAGTATCCGTTTCTGCTTTccg > 1:117153/1‑250 (MQ=255) tAAAAATACCGGAAATCCTCAAGCTCCAGGTACGATCATTGGCGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGAGCGCGTGTTTGCAGCGAGGTCACGAGCCCGTATTTCCGTGGAGCTGATTACGCCATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCg > 1:523969/1‑251 (MQ=255) aaaaaTACCGGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCg > 1:543366/1‑250 (MQ=255) aaTACCGGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGCCtg > 1:241550/1‑251 (MQ=255) ccTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGAGCGCGTGCTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAAACCTCTTCCGAATACAGTATCAGTTTCTACGGTTCGCAAAGCGACTGTGTGCgggccg > 2:501096/1‑245 (MQ=255) cAGGTACGCTCATGGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTGCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATg < 2:284096/251‑1 (MQ=255) aGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGc > 1:321352/1‑251 (MQ=255) ggtggctgcctgtattaaGACGAACTACCGGGCAAGGGCATTTCCAAGCTGAATAATATGCAAATGTTCAGCGTGTCCTGTCCGGGGATGAAAGGGATGGTTGGCTGGGCGGCGCGCTGGGTTACATCAAGGTCACGCTCCCGTATTACCGTGGTGCTGATTACGCAATCATCTTCCGACTACAGGATCAGTTTCTGCTTTCCGCAAAGCGACTGGCTGCGAGCTGAACGGGCAATGCAGCAagtggtcta < 2:250280/235‑8 (MQ=255) ccgggatgaatgcgcatgacctgTCAAGGGCTTTGCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCTTGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCTCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGGGCGAGCTGAACGGGCAAGGCAGGAAGAGTTCTTCCTGGAAc < 2:551203/229‑1 (MQ=255) tgatgaAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGAGTGTTTTCAGCGGTGTCCCGCGCCCGGATTTCCGTGGGGCTGGTTACGCAATCATCTTCCGGATACAGGATATGTTTTTGCGTTCCGCAAAGCGACTGTGTGGGCGCTAGACGGGGAATGCCGGAagggatctcaccgggactg > 2:13668/1‑233 (MQ=255) aTTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCTGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTAc < 1:245272/251‑1 (MQ=255) acctgTCAAGGGCATTTCCAAGCAGAATAATATGGCAATGTGCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCGTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTg < 2:239904/246‑1 (MQ=255) aCCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGACAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGGGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGACGAGTTCTACCTGGAACTGCaagacagcttcatgg > 2:35666/1‑240 (MQ=255) aCCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCCATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTgg > 1:324984/1‑251 (MQ=255) aaGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTGCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCt < 1:368464/251‑1 (MQ=255) gggCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACggg > 1:644821/1‑197 (MQ=255) gggCAGTTCCAAGCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACggg < 2:644821/197‑1 (MQ=255) ttCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTgg > 1:552754/1‑250 (MQ=255) tCCAATCTGAATAATATGGCAAGGTTCATCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTTCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGa < 1:394236/250‑1 (MQ=255) ccAATCTTAATAATATGTCAATGTGCAGCGTTTCCGGCCCGGGGATTAAAGGGATGGTTTGCATGGGGGCGCGCGTGATTTCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCACTCATCATCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGAAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTTGCGGTGAc < 2:594500/250‑1 (MQ=255) aaTCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGGGAC‑ggg > 1:340465/1‑250 (MQ=255) aaTCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGGTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCa > 1:246934/1‑189 (MQ=255) aTCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGCTGACACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCCATGCAGGAAGAGGTTTAACTGGAACTGAAAGAAGGCTTACAGGGGCCGCTGGcgggggggg > 2:87618/1‑241 (MQ=255) aTCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTgg < 1:643848/210‑1 (MQ=255) aTCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGGATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAACGACTGTGTGCGAGCTGAACGGGCACTGCAGGAAAAGTTCTACCTgg > 2:643848/1‑210 (MQ=255) ataataTGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTg < 1:35666/251‑1 (MQ=255) ataataTGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTg < 2:266950/251‑1 (MQ=255) ataTGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGGATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGcc > 1:251315/3‑251 (MQ=255) taTGGCAATGTTCAGCGTTTCCGGCCCGGGGCTGCACGGGCTGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGCTGTCCCGCGCCCGTATTTCCGTGGTGCTGGTTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGGACTTAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACTGGAACgcttgccc > 1:254445/2‑244 (MQ=255) ggCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCAtt < 1:604702/250‑1 (MQ=255) cAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGGC‑GGAAAGGcggggcactttt > 2:237356/1‑240 (MQ=255) tGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGTTTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGGGTGCGAGCTGAACGGGCAATGAAGGAAGAGTTCTACCAGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGGGGC‑GGGACGGCTgggcacttatt > 2:570280/1‑240 (MQ=255) cAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAGATGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGGACGGCAGGCCATTATCTCgaggg > 1:24906/1‑247 (MQ=255) agcgtgtccggcccGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCggtggt < 2:302104/239‑1 (MQ=255) ccggcccGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGAt < 1:123992/246‑1 (MQ=255) ggcccGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGGCATTATTTCGGTGGG‑AGG‑GGATg‑g > 1:374563/4‑251 (MQ=255) cccGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGAGAC‑GGAACGGCTGGCCATTCACTCGGTGGT‑AGG‑GGATg‑gg > 1:198460/2‑249 (MQ=255) ccGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GTAt < 1:3999/251‑1 (MQ=255) ccGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GTAt < 1:323048/251‑1 (MQ=255) ccGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGGGAC‑TGGACGGCTGGCCCTTATCTCGGTGGT‑AGG‑GGATG‑GTAt > 1:319608/1‑251 (MQ=255) gAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTTTCTCGGGGGT‑AGG‑TGATG‑GTATGCGCACCt > 1:606164/1‑251 (MQ=255) gggtggCAGGGCGGCGCGCGTGGTTGCTGAGATATGACGCGTCCGTATTGCCGTGGTGCTGATTGCGCAATCATCTTCCGAATACAGTATCAGGTTCCGCGTTCCGCAAAGCGACTGGGTGCTAGCTGAACGGGCCATGCAGGAGGAGTTCTACTGGGAACTGAAAGAAGGCTTACTGGAGCCGCGGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GTATGCGCACCTTGCGTGGGa < 2:162740/247‑1 (MQ=255) ggCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GTATGCGCACCTTGCGTGGGAtct < 1:570280/250‑1 (MQ=255) ggCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCACGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GTATGCGCACCTTGCGTGGGAtctc < 1:343346/251‑1 (MQ=255) cggcgCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCG‑aaa < 1:196652/251‑1 (MQ=255) gcgTGTTTGCAGCGATGTCACGCGCTCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATGCAGTATCAGTTTCTGCGTTCCGCAAAGCGACCGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCG‑AAATTCttt < 1:643932/251‑1 (MQ=255) gtttGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGACTACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGc > 2:232861/2‑168 (MQ=255) gtttGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGc < 1:232861/167‑1 (MQ=255) gtttGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGGACGGATGGCCATTAACACGG‑GGTGAGGGTG‑TT‑GTATGCGCACCATGGGTGGGATTTCGGCGCAAATT‑TTTGccc > 2:268561/2‑250 (MQ=255) tttGCAGCGATGTCACGCGCCCGTATGTCCTTGGTGGTGATTACGCAAGCGTCTACCGAATGCAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCGGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCG‑AAATTCTTTGCCg < 2:164807/250‑1 (MQ=255) aGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCt > 1:257070/1‑100 (MQ=255) aGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTGCCGCAAAGCGACTGTGTTCGAGCt < 2:257070/100‑1 (MQ=255) aCGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑GGATG‑GTATGCGGAACTTGCGTGGGATCTCGGCG‑AAATTCTTTGCCGCGCTGGCCCGCGccc > 1:10537/1‑250 (MQ=255) gTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCG‑AAATTCTTTGCCGCGCTGGCCCGCGCCAATATCAAc < 1:150850/251‑1 (MQ=255) ttCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GGATGCGCACCTTGCGTGGGATCTCGGCG‑AAATTCTTTGCCGCGCTGGCCCGCGCCAATATCAACATTg > 1:93488/1‑251 (MQ=255) ccGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑GGATGTGT‑TGCGCACCTTGCGTGGGATCTCGGCG‑AAATTCTTTGCCGCGCTGGCCCGCGCCAATATCACCATTGTc > 2:110947/1‑251 (MQ=255) gtggtgCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAaaga < 1:461598/116‑1 (MQ=255) gtggtgCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAaaga > 2:461598/1‑116 (MQ=255) gtggtgCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GTATGCGCAACTTGCGTGGGGTCTCGGCG‑AAATTCTTTGCCGCGCTGGCCCGCGCCAATATCAACATTTTC‑Gc > 1:655808/1‑251 (MQ=255) aCGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCG‑AAATTCTTTGCCGCGCTGGCCCGCGCCAATATCAACATTGTC‑GCCATTGCTCAgg‑g < 1:8296/251‑1 (MQ=255) cGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCG‑AAATTCTTTGCCGCGCTGGCCCGCGCCAATATCAACATTGTC‑GCCATTGCTCAgg‑g > 1:288402/1‑250 (MQ=255) cGCAATCATCTGCCGAATACGGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCG‑AAATTCTTTGCCGCGCTGGCCCGCGCCAATATCAACATTGTC‑GCCATTGCTCAGG‑Ga < 2:340465/251‑1 (MQ=255) cAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGG‑AGG‑GGATG‑GTCAGGGCACCCTTCGGGGGATCTCGGCG‑AAAATCTTTGCCGCCCTGGCCCCCCCCAAGAACCACATTGTCCGCC‑TTGCTCAgg‑ggt > 2:290569/1‑248 (MQ=255) atcTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑GGATG‑GGATGCGCACCTTGCGTGGGATCTCGGCG‑AAATTCTTTGCCGCGCTGGCCCGCGCCAATATCAACATTGTC‑GCCCTTGCTCAGG‑GATCTTCTg > 1:400836/1‑251 (MQ=255) ctCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCG‑AAATTCTTTGCCGCGCTGGCCCGCGCCAATATCCACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAAc > 1:193722/2‑251 (MQ=255) tCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCG‑AAATTCTTTGCCGCGCTGGCCCGCGCCAATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGa < 1:352537/248‑1 (MQ=255) ccGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCG‑AAATTCTTTGCCGCGCTGGCCCGCGCCAATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACg < 1:274611/250‑1 (MQ=255) aaTACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGATCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGG‑AGG‑GGAGG‑GGAAGAGAACCCTGAGTGGGCTATCGGCG‑AAAATCTTTGCCGCGCTGGCCCGCGACAAAATAAACAATGTC‑GCACTTGCTCAGGAGA‑CTTCTGAAcccacc > 2:439439/1‑246 (MQ=255) aCAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCG‑AAATTCTTTGCCGCGCTGGCCCGCGCCAATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAAtc < 1:237356/251‑1 (MQ=255) | TCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAACATGGCAATGTTCAGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGT‑AGG‑TGATG‑GTATGCGCACCTTGCGTGGGATCTCGGCG‑AAATTCTTTGCCGCACTGGCCCGCGCCAATATCAACATTGTC‑GCCATTGCTCAGG‑GATCTTCTGAACGCTCAATC > NC_000913/1169‑1653 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |