Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 11,861 C→T intergenic (‑75/‑302) yaaI ← / → dnaK DUF2541 domain‑containing protein YaaI/chaperone protein DnaK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091311,8610CT92.1% 95.2 / ‑2.1 39intergenic (‑75/‑302)yaaI/dnaKDUF2541 domain‑containing protein YaaI/chaperone protein DnaK
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base T (18/17);  total (21/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

TTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACTAGTTTACTGCTGATAAAGAG‑AAGATTCAGGCACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATATGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGACGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCA  >  NC_000913/11629‑12097
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                            
ttCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCATTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCCCAGCATCAGGCTAATCGCCAGGCTGGCGGGAATCGTAACAACGAAGTTCATAAGGCTTCTCTTAGTGGGAAGAGGAAGAGGGTTGAATTCCCACTAGTTTCTTGATGATATAGAA‑AAGGTTCAGGTACGGAATCTTTTCttttt                                                                                                                                                                                                                            >  2:495844/1‑251 (MQ=255)
           aCAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAG‑AAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAAtttt                                                                                                                                                                                                                 >  2:676361/1‑251 (MQ=255)
               ccAGGAAGGTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGTCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAG‑AAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTtga                                                                                                                                                                                                             <  1:43514/250‑1 (MQ=255)
                    aaGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAG‑AAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAAt                                                                                                                                                                                                        <  1:264661/251‑1 (MQ=255)
                        ttCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGGTTTCATAAGGATTCTCTTAGTGGGAAGAGGGCGAGGGATGAATACCCACTAGTTTACTGCTGGTAA‑GAGGAAGGTTCAGGTACGTAATCTTTTTTTTTTATTACACTTTTTTGATGAAGGCCt                                                                                                                                                                                                    >  2:473955/1‑251 (MQ=255)
                           aGCGCCAGATCGATGGTTTCGTTACGCGGCAGTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTACTCGCCAGGCTGGCGGCAGTCGTAAAAACGGTTTTCATCAAGATTCTCTTAGTGGATAGAGGTAGAGGGTTGATTCCCCACTCGTTTACTGCTGATAAAGAG‑ACGGTTAAGGAACTTACTCTTGTTTTTTTTTTACAATTTTTTGATGAATTCCtttg                                                                                                                                                                                                 >  2:533352/1‑249 (MQ=255)
                            gcgcCAGATCGGTGGTTTCGTTACGCGGCATTGCAATGACGACGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAG‑AAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGc                                                                                                                                                                                                <  2:171420/251‑1 (MQ=255)
                             cgcCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAG‑AAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCt                                                                                                                                                                                               <  1:196838/251‑1 (MQ=255)
                                        tGGTTTCGTTACGCGTCATTGCAATAACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGACAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCCCTAGTTTACTGCTGATAAGGAG‑AAGGATAAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGattcattc                                                                                                                                                                                    >  2:424231/1‑251 (MQ=255)
                                          gttgcGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATTGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAG‑AAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCtt                                                                                                                                                                                  <  1:473471/247‑1 (MQ=255)
                                              cGTTACGCGGCATTGAAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAG‑AAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCTATTCATTCTTtat                                                                                                                                                                               <  1:537259/250‑1 (MQ=255)
                                                ttACGCAGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAG‑AAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTtatttg                                                                                                                                                                            >  2:652294/1‑248 (MQ=255)
                                                  aCGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGCCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACTAGTTTACTGCTGATAAAGAG‑AAGATTCAGGCACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATATGaa                                                                                                                                                                          <  1:89729/251‑1 (MQ=255)
                                                        cATTGCAAAGACGCCGAGGAGTTTATGGACGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTACAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAG‑AAGGTTCAGGCACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAAt                                                                                                                                                                    >  1:680082/1‑251 (MQ=255)
                                                                       gaggagTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAG‑AAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCTGTCAATTTTAc                                                                                                                                                     <  1:544046/251‑1 (MQ=255)
                                                                                               cgcCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAG‑AAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCTGTCAATTCTACGTCTTTTCCTGCCATATCGCGaa                                                                                                                              >  2:460808/1‑250 (MQ=255)
                                                                                                gccgtgacgagcagagtCAGGCTAATAGCCGCGCTCGCGGAAATCGTAAAAACGGGTTTCATAAGGATACTCTTAGTGGGAAGAGGTAGAGGGGTAAATACCCACTAGTTTACTGCTGATAAAGAG‑ACGGTTCAGATACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCATTGGATGCGATTCATTCTTTATCTGAATAAAATTGCTGTCAATTTTACGTCTTATCCTGCCATATCGCGGAAt                                                                                                                            >  2:83223/17‑251 (MQ=255)
                                                                                                                              aGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAG‑AAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGc                                                                                               >  1:9487/1‑250 (MQ=255)
                                                                                                                                  tGGCGGAAATCGTAAAAACGTAGTTCATAAGGGTTCTCTGAGTGGGAAGAGGGAGGGGGATGAATAACCACTAGTTTACTGCTGATAAAGAG‑AGGATTCAGGCACGTAATCTTTTCTTTTTATAACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATATGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCGGCACAAAAGCACAAAAAATTTTTGCAtatc                                                                                          >  2:192684/1‑248 (MQ=255)
                                                                                                                                     cGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAG‑AAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTg                                                                                                                                                                  >  1:661560/1‑176 (MQ=255)
                                                                                                                                     cGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAG‑AAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTAGCTGAATAAAATTg                                                                                                                                                                  <  2:661560/176‑1 (MQ=255)
                                                                                                                                        aaaTCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAG‑AAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCtt                                                                                    <  1:460808/251‑1 (MQ=255)
                                                                                                                                             gTAAAAACGGATTGCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAG‑AAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTtgatga                                                                               <  1:495844/251‑1 (MQ=255)
                                                                                                                                                 aaaCGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAG‑AAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGACGTg                                                                           >  1:358792/1‑251 (MQ=255)
                                                                                                                                                          tCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTATTTTACTGCTGATAAAGAG‑AAGGGTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGACGTGGGTTACGa                                                                   <  1:473955/250‑1 (MQ=255)
                                                                                                                                                              atgtatcctctgagggggAAGATGTATAGGGATGACTACACTCTATTTTACTGCTGCTAAATAG‑AAGGTTTAGGTCCTGATTCTTTTCTTTTTAGTCAAATTTTTTGATGAATGCTTTGGCTGTGTTTCATTCTTTATCTAAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGATATTGCTGCGCAAAAGCACAAAAAATTTTTGCATCTCTCCCTTGATGACGTGGTTTTCGAcccc                                                               <  2:435430/235‑1 (MQ=255)
                                                                                                                                                                     ctctTAATGAGACGAGGTAGAGGGATGAATACCAACTAGTTTACTGCTGATAAAGAG‑AAGGATCAGGTAAGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGCTTAATTCTTTATCTGAATAAAATTGCTGTCAATTTTACGTATTGTCCTGCAATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGACGTGGGATACGACCCCATTaagt                                                        >  2:627679/1‑250 (MQ=255)
                                                                                                                                                                         tAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAG‑AAGTTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGACGTGGGTTACGACCCCATTtagtag                                                      <  1:152350/248‑1 (MQ=255)
                                                                                                                                                                                aaGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAG‑GAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGACGTGGGTTACGACCCCATTTAGTAGTCAActgc                                              >  2:501075/1‑246 (MQ=255)
                                                                                                                                                                                     ttagagggATGAATAACCACTAGTTTACTGCTGATAAAGAG‑AAGATTCAGGTACGTAATCTTTTCTTTTTAGTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAACTTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGACGGGGGTTACGACCCCATTTAGTAGTCAACCGCagtaagt                                       >  2:160228/6‑251 (MQ=255)
                                                                                                                                                                                      tagagggATGAATACCCACTAGTTTACTGCTGATAAAGAG‑AAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGACGTGGGTTACGACCCCATTTAGTAGTCAACCGCagtgagtg                                      >  2:492061/5‑251 (MQ=255)
                                                                                                                                                                                              ggAATACCCACTAGTTTACTGCTGATAAAGAG‑AAGGTTCAGGTACTTAATCTTTTCTTTTTATTACAATGTTTTGATGATTGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCGTGATGACGTGGGTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCa                              <  1:86248/250‑1 (MQ=255)
                                                                                                                                                                                                   aCCCACTAGTTTACTGCTGATAAAGAG‑AAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGACGTGGGTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCaaaaa                          >  1:113325/1‑250 (MQ=255)
                                                                                                                                                                                                    ccctcTAGTTTACTGCTGATAAAGAG‑AAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTGGATGAATGCCTTGGCGGCGTTTCATTCTTTAGTGGAAAAAAATTGCTGCCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGACGTGGGTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCaaaaaaa                        <  2:227507/247‑1 (MQ=255)
                                                                                                                                                                                                          aGTTTACTGCTGATAAAGAG‑AAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGACGTGGGTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATGaa                    <  1:676361/249‑1 (MQ=255)
                                                                                                                                                                                                                 tgctgATAAAGAG‑AAGGGTCAGGTACGTAATCTTTTCTTTTTATTACAATTCTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGACGTGGGTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGc             >  1:191576/1‑249 (MQ=255)
                                                                                                                                                                                                                         aaGAG‑AAGGTTCAGGTACTTATTTTTTTCTTTTTATTACAATTTTTGGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCTGTCAATTTTACGTCTTTTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGACGTGGGTTACGACCCCATTTAGTAGTCa                                                   <  2:644126/203‑1 (MQ=255)
                                                                                                                                                                                                                         aaGAG‑AAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGACGTGGGTTACGACCCCATTTAGTAGTCa                                                   >  1:644126/1‑203 (MQ=255)
                                                                                                                                                                                                                           gag‑aAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGACGTGGGTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGCGTCTGCAAAAAAATGAAATCGGGCAGTTGAAAccg  >  2:154278/1‑249 (MQ=255)
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                            
TTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACTAGTTTACTGCTGATAAAGAG‑AAGATTCAGGCACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATATGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGACGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCA  >  NC_000913/11629‑12097

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑