Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 11,439 C→G S116S (TCG→TCC)  yaaI ← DUF2541 domain‑containing protein YaaI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091311,4390CG95.5% 123.6 / ‑0.1 44S116S (TCG→TCC) yaaIDUF2541 domain‑containing protein YaaI
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base G (21/21);  total (21/23)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.85e-01

TGCCCTTCCATCGATTTAAACAGTTCGTTGCGCATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGCTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCCGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCGCTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGGCTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTA  >  NC_000913/11193‑11679
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                
tGCCCTTCCATCGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGaa                                                                                                                                                                                                                                               >  1:374904/1‑250 (MQ=255)
  cccTTCCATCGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAg                                                                                                                                                                                                                                            <  1:108775/251‑1 (MQ=255)
     ttCCATCGATTTAAACAGTTCGTTGCGTATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTg                                                                                                                                                                                                                                         >  1:161398/1‑251 (MQ=255)
       tcATCGATTTAAACAGTTCGTTGCCTACCAGTTCGCTGTAGAGGCGCGTTGTGCCTTTTTCATTATTGGTGAGCAATCTGTCGAAATTTGCCATCTGTTCCTCCCTACAATGCTGCAGAAAATCCAGATCCGACTCATTCAGTTAATTACCATTCATTTTTTGTGTTTTCTATATTCTGGCGGTAGCCGTCGCCGATAATTTTCAGCGTGGCCATATCCTCTGAGTTCTCCGTATGATCGGAAAAGGTGa                                                                                                                                                                                                                                        <  2:72226/249‑1 (MQ=255)
                tAAACAGTGCGTTGCGCATCATTACGCTGGAGAGGCGAGCTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTTAAGAAAATCCAGATCTTAATCATTCAGGTAATTAACATTCAGTTTTTGTGGCTGCTATATTCTGGCGTTAGTCATCGCCGATAATTTTCAGCGTGTCCAGATCCGATGAGTTCACCGTATGACCCGAAAAGGTGATTTTTgaga                                                                                                                                                                                                                               <  2:67257/250‑1 (MQ=255)
                          gTTGCGTATCAGTACGCGGGAGAGGCGAGTTTTGCCTTGTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTa                                                                                                                                                                                                                    <  1:61306/251‑1 (MQ=255)
                              cgtaTCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCTATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGt                                                                                                                                                                                                                >  2:309258/4‑251 (MQ=255)
                                     gTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATc                                                                                                                                                                                                         <  2:161398/251‑1 (MQ=255)
                                              atagtagAGTTTTGCCTTATTCATTATGGTTGAGCAATCTGGCGAAATTTGCCATCTGTTCTTCACTACAATTCTGAAGAAAAGCCAGATCTGAATCATTCAGGTAATTAACATACATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCTATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGTCCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTTTCACTGTTAAt                                                                                                                                                                                                <  2:429904/245‑1 (MQ=255)
                                                agGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGCAGAAAATCCAGATCTGAATCATTCAGGTACTTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGCGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCGCTGTTACTGt                                                                                                                                                                                              >  1:499484/1‑251 (MQ=255)
                                                    gaattttGCCTTTTTCATTACGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTACTCACTACAATGCTGAAGAAAAGCCAGATCTGAATCATTCAGGTAATTAACAGTCATTTTTGGTGGTTTCTATATGCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGAt                                                                                                                                                                                          <  1:5061/248‑1 (MQ=255)
                                                      gTTTTGCCTTTTTCATGATGGGTGAGCAATCGGTCGAAATTTGCCAACTGTTCCTCACTACAATTCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATAGTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATc                                                                                                                                                                                         <  2:638642/250‑1 (MQ=255)
                                                               ttttCATTATGGGTGTGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTTAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGTCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATTACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCcagtcagt                                                                                                                                                                                 <  2:38436/249‑1 (MQ=255)
                                                                 ttCATTATGGGTGAGCAATCGGGCGAAATATGCCACCTGTTCCTCCCTACAAAGCTGAAGAATATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGAGGTTTCTATATTCTGGCATTAGTCTTCGCCGATCATTTTAAGCGTGTCAATATCCGCTGAGTTCACCGTATGCACGGTAAAGGTGATTTTTGATACGCAGCGTTTATTGACATTATATCTGTTAATTTTTATCCAGTAAGTgatt                                                                                                                                                                             >  2:473471/1‑248 (MQ=255)
                                                                   cATTATGGGCGAGCAATCGGGCGAAATTTGCCAACTGTGCCTCACTACAATGCTTAAGAAAATCCAGAGCTGAATTATTCAGGTAATTAACATGCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGttt                                                                                                                                                                            <  2:381524/250‑1 (MQ=255)
                                                                   cATTATGGGAGAGCAATCAGGCGAAATATGCCAACTGTTCCTCACTCCAATGCTTAAGAAAACCCAGTTCTGAAACATTCAGGTAATTAACATTCATTTTTTGTGGGTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCTACGAGTTCACCGTATGACCGGAAAAGGTGATTTTCTAGACGCAGCGTTTATTTCCGTTATCACTGTTAATTTTGATCCAGTCAGTGGttt                                                                                                                                                                            <  2:348685/250‑1 (MQ=255)
                                                                             gAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCtttt                                                                                                                                                                 >  1:104789/1‑251 (MQ=255)
                                                                                  aTCGGGCGAAATTAGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGACTCATTCAGGTAATTAACATTCATTTTTTGTGATTTCTATATTCTGGAATTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGTTGAGTTCACCGTATGACCGGAAAAGGTGATTTTTTAGACGAAGCGTTTCTTGTCGTTATCACTGTTAATGTTTATCCAGTCAGTGGTTTGCCCTTCTTTTAtttt                                                                                                                                                            >  2:537259/1‑250 (MQ=255)
                                                                                     gggCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTc                                                                                                                                                            >  2:179599/1‑248 (MQ=255)
                                                                                     gggCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTc                                                                                                                                                            <  1:179599/248‑1 (MQ=255)
                                                                                     gggCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTg                                                                                                                                                          >  1:600325/1‑250 (MQ=255)
                                                                                      ggCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGaa                                                                                                                                                        <  1:195586/251‑1 (MQ=255)
                                                                                                      tGTTCCTCACTACACTGCTGCCGAAACTCCAGATCTGAATCATTCAGGTCATTAACATTCATTTTTTGTGGTTTCTATATTCTTGCGATAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTCTGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGTAGGATTATTCAGActct                                                                                                                                        >  2:94184/1‑251 (MQ=255)
                                                                                                        ttCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCaaactctg                                                                                                                                       >  2:264661/1‑243 (MQ=255)
                                                                                                           cTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTg                                                                                                                                   >  1:676304/1‑251 (MQ=255)
                                                                                                                   aaTGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACg                                                                                                                           <  1:388952/251‑1 (MQ=255)
                                                                                                                                   cAGATCGGAATCATTCAAGTAATTAACATTCAGTTTTTGTGGTTTCTATATTCTGGCGTTAGGCGTCGCCGAGAATTTTCAGCGTGTCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTTAGTGGTTTGCTCTGCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGTTACGGGCGGCTTTGAAATaa                                                                                                           <  2:618039/251‑1 (MQ=255)
                                                                                                                                                 tCAGGTAATTAACATTCATTTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAATTAAACCGATgacccgct                                                                                             >  1:435430/1‑244 (MQ=255)
                                                                                                                                                      tAATTAACATTAATTTTGTGTGGTTTCTATATTCTGGCGTTAGTCGTAGCCGATAATTTTCAGCGTGTCCATATCCGATGAGTTCACCGTATGAACGGAAAAGGTGTTTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGATGCTCCAGTCAGTGGTTTTACCTTCTTTTATTTCTGATGGAATATACAGACTCTGACTGGCGCTACGGGCGGCTTTGACATACACCGATTCACCGTTTAACt                                                                                        >  2:152350/1‑251 (MQ=255)
                                                                                                                                                         ttAACATTCATTTTTTGTGGCTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCCGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCGCTGTTAATGtt                                                                                                                                                                                             <  1:121627/147‑1 (MQ=255)
                                                                                                                                                             cattcattTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCAAATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGAGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATc                                                                                 <  1:433074/251‑1 (MQ=255)
                                                                                                                                                              atacaatctttGTGGTTGCTATATTATGGCGTTAGTCGACGCAGATATTTTTCATAGTGGCCATATTCGATGAGTTCACCGTATGCCAGGAATAGGTGAGTTTTGAGACGCAGCGCTTATTGTCGCTATCACTGTGAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTAGTTCTGCAGGAATATTCAGCCTCTGATTGGCGCTACGAGCGGCTTTGTAATAAACCGATGCACCGATTAACTGTatctcg                                                                                >  2:282005/9‑246 (MQ=255)
                                                                                                                                                                 cattTTTTGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGcc                                                                              >  1:317993/1‑250 (MQ=255)
                                                                                                                                                                    tttttGTGGTTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATg                                                                           <  1:59572/250‑1 (MQ=255)
                                                                                                                                                                        tgtgGTTTCTATATTCTGGAGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCTTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGTTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCg                                                                       <  2:54947/250‑1 (MQ=255)
                                                                                                                                                                                              tAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCCGCGTTTATTGTCGTTATCCCTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGACGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACAGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTcac                                                >  1:416217/1‑251 (MQ=255)
                                                                                                                                                                                                     cgCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCg                                          >  1:48544/1‑250 (MQ=255)
                                                                                                                                                                                                          aTAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGCCCGGTAAAGGTGATTTTTGAGACGCAGCGATTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGCCAAAc                                    >  2:108446/1‑251 (MQ=255)
                                                                                                                                                                                                          aTAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAAc                                    <  1:108446/251‑1 (MQ=255)
                                                                                                                                                                                                                     ggTGGCCATATCCGATGAGTTCAACGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGGTAATGTTGATCCAGTCAGTGGGTTGCCCTTCGTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCa                         <  2:676304/250‑1 (MQ=255)
                                                                                                                                                                                                                        ggCCATATCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAgcg                      <  2:104789/251‑1 (MQ=255)
                                                                                                                                                                                                                            atatCCGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCTATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTTTATGCGTTTCACAATGCGACCAACAGGAAGTTTCAGCGcc                    >  2:436019/1‑249 (MQ=255)
                                                                                                                                                                                                                                ccGATGAGTTCACCGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCg              <  1:309258/251‑1 (MQ=255)
                                                                                                                                                                                                                                             cGTATGACCGGCAATGGTTATTTTTGAACCTCAGCGTTTATTGTCGTTATCAATGTTAATGTTGATCCAGTCAGTGGTTTGCACTTCTTTTATTTATGAAGGAATATTCAGCCTCTGACTGGCGCTACGGGCGGCATTGCAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTAGGCAGAGAGATGTGTGCGTTTCACAATGCGCCAAACAGGAGGTTTCAgcgaacgctggtgggtttcttta  >  2:86248/1‑230 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                
TGCCCTTCCATCGATTTAAACAGTTCGTTGCGCATCAGTACGCTGGAGAGGCGAGTTTTGCCTTTTTCATTATGGGTGAGCAATCGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTAACATTCATTTTTTGTGGCTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCCGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCGCTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGGCTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTA  >  NC_000913/11193‑11679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑