Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	10,985	A→G	N124N (AAT→AAC)  V52V (GTA→GTG)	yaaW ← mbiA →	putative enzyme‑specific chaperone YaaW uncharacterized protein MbiA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	10,985	0	A	G	100.0%	204.0 / NA	65	N124N (AAT→AAC)  V52V (GTA→GTG)	yaaW mbiA	putative enzyme‑specific chaperone YaaW uncharacterized protein MbiA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/37);  total (28/37)

CCCCATTTAGTGCCGCACCTACAGGGCCTCCCAGCCCCGCGCCGCGCAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACACCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATTTTCTTCCAGGTATTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATTTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGCATCAGT  >  NC_000913/10738‑11231                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       ccccATTTAATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTa                                                                                                                                                                                                                                                     <  1:594424/251‑1 (MQ=255)  cccATTTAATGCTGCGCCCACAGGGCATCCCAGACCCGCGCCGCGCAGCAAACCACGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCCTCGCCCGCGCGCAGACACTCCAGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTAc                                                                                                                                                                                                                                                    >  2:366859/1‑251 (MQ=255)                                cagacccGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCaa                                                                                                                                                                                                                      <  1:589966/247‑1 (MQ=255)                                   ccccGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAAtct                                                                                                                                                                                                                   <  1:366859/251‑3 (MQ=255)                                       gcgccgcgcAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCaaa                                                                                                                                                                                                               <  1:86445/251‑1 (MQ=255)                                           cgcgcAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGt                                                                                                                                                                                                            >  1:298110/1‑250 (MQ=255)                                               cagcaAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGCCCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCACATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGCTGCTCAATATCAAACGTAGAc                                                                                                                                                                                                        >  1:66848/1‑250 (MQ=255)                                               cagcaAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGGGTTACGCAGCACTTGTTCCAGTAACTGTTGCTCAATCTCAAACTTAGAc                                                                                                                                                                                                        >  2:342096/1‑250 (MQ=255)                                                   aaaCCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGCCCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACAtctc                                                                                                                                                                                                   >  2:264673/1‑251 (MQ=255)                                                          gCCCAAGCACGCTCATTGCTGCGTGGGTGCGTCAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGCAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATATCTTTGTCg                                                                                                                                                                                            >  2:60940/1‑251 (MQ=255)                                                          gCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCg                                                                                                                                                                                            <  2:421635/251‑1 (MQ=255)                                                                 caCGCTCATTGCTGCGTGGGGGCTTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGAAACGCCTTTTGCCAATAATTTGGCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGAAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGTCTTTCa                                                                                                                                                                                     <  2:477370/250‑1 (MQ=255)                                                                    gCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCt                                                                                                                                                                                  >  2:218760/1‑251 (MQ=255)                                                                      tCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCCGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTc                                                                                                                                                                                >  2:617259/1‑251 (MQ=255)                                                                       cATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCtt                                                                                                                                                                                 <  1:202153/249‑1 (MQ=255)                                                                        aTTGCTGCGTGGGTGCGAAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCa                                                                                                                                                                               <  2:400146/250‑1 (MQ=255)                                                                           gctgcGTGGGTGCGTAAAATGCGGGTCAATTGTCTGGAAAGCAAAGGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAATCTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACGGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCg                                                                                                                                                                           <  1:601720/251‑1 (MQ=255)                                                                            ctgcGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGCAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATATCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCgt                                                                                                                                                                          >  1:323740/1‑250 (MQ=255)                                                                            ctgcGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCgt                                                                                                                                                                          >  1:456523/1‑250 (MQ=255)                                                                             gtcgggggggtgTAAAATGCGTGTCAAATGGCTGGAGAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCAACAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGGAGACATCACTTTGTCGGCTTTCAGCTTCAATCgtt                                                                                                                                                                         <  2:351970/240‑3 (MQ=255)                                                                               cGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTGTGTCGGCTTTCAGCTTCAATCGtttt                                                                                                                                                                       <  1:264673/251‑1 (MQ=255)                                                                                  gtgTGCGTAAAATGCGGGACAAATGTCTGGAAAGCAAATGCGACACGTCTTTTGCCAATTAATTGTTTGTCATCAGCAGCGGCAGCAGCTCTTCCAGGTCATTCACCCTGGCATCGACCGCGTGCAGACACTCCGTCTTATGTTCCTCGTCCTTATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACTTAGACATCTCTTTTTCGTATTTCAGCTTCAATCGTTTTGaa                                                                                                                                                                    <  2:579250/249‑1 (MQ=255)                                                                                          aaaaTGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAAGTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGa                                                                                                                                                             <  1:344835/250‑1 (MQ=255)                                                                                               gCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCaaaa                                                                                                                                                       <  1:616718/251‑1 (MQ=255)                                                                                                  ggTCAATTGGCTGGAAAGCAAATTCTACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGAATCTCTTCCAGCTCATGCACCCTGGCATCGACCGCGTTCAGAAACTCCTGCTTATGTTCCTCTTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCGCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACAGCGAGCAAAATgg                                                                                                                                                    <  2:671098/251‑1 (MQ=255)                                                                                                           gCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCCGCAGCTCTTCCCGCTCCTTCACCCTGGCATCGACCGCGTGCCGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGCGTTAAGCAGAAATTGTTCCAGTAACTGTTGCTCACTCTCAACCGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATAGTTTTGAAACATCGAGCAAAATAGCCCTATAcc                                                                                                                                           >  2:215341/1‑250 (MQ=255)                                                                                                            cTGGAAAGCAAAAGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTCGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCAAGCAAAATGGCCCGATACa                                                                                                                                           >  2:423824/1‑250 (MQ=255)                                                                                                             tGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAAt                                                                                                                                         >  1:73109/1‑251 (MQ=255)                                                                                                                 aaGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTAc                                                                                                                                     >  1:617612/1‑251 (MQ=255)                                                                                                                     taaTGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCCGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGTCCGCGTGCAGAAAATCATGCTTATGTTCCTCGTCCATATACTTCCCGGGGTTACCAAGAACGTGTTACAGTTACTGTTGATCGATCTCCAACGCAGAAATCTCTTAGTCTGCTTTAAGCTTCAATCGTGTTGTAACATCGAGAACAATGGCACGATACACTTTACCgtt                                                                                                                                 >  2:185307/2‑250 (MQ=255)                                                                                                                                 ccTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAGCATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGttt                                                                                                                     >  1:638642/1‑251 (MQ=255)                                                                                                                                     ttGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTATACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTATCGTATCCGCGCAGTttgttt                                                                                                                 >  2:182749/1‑250 (MQ=255)                                                                                                                                      tGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCTCCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTttgttg                                                                                                                 <  1:580999/250‑1 (MQ=255)                                                                                                                                             aatTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACt                                                                                                         <  1:218760/251‑1 (MQ=255)                                                                                                                                                   ccTTTCATCAGCAGCGGAAGCAGCTCTGCCAGCTCATTCACCCTTGCATCGACCGCGGGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGGTTGTTGGCGATACTATCGca                                                                                                   <  2:456523/250‑2 (MQ=255)                                                                                                                                                      ttCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGcca                                                                                                  <  1:573100/249‑1 (MQ=255)                                                                                                                                                         atcaGCCGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCTCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCTAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTTTTGGCGGTTCTATAGCCACCaa                                                                                              >  2:61306/1‑250 (MQ=255)                                                                                                                                                         atcaGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAACTGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCaa                                                                                              >  2:108775/1‑250 (MQ=255)                                                                                                                                                                  ggCAGCAGCTCTTCCAGCTCATTCACCCTGGCAGCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCTTGTCCGCGCAGTGTGTTGGCGATACTATCGCCACCAAAATGCTGTaa                                                                                    <  1:622933/251‑1 (MQ=255)                                                                                                                                                                       cagatctTCCAGCTCAGTCTCCCTGGCTTCGAGTGCGTGCAGAAACTCCTGCTTATGTTCCTCGCCCATATTCTGCCAGGGGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTATACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCTAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGGGGGCGATACTTTCTCCACCAAAATTCTGTAATtctc                                                                               <  2:175103/247‑1 (MQ=255)                                                                                                                                                                           tctTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGACACATCGAGCAAAATGGCCCGCTACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTccagc                                                                           >  1:314550/1‑248 (MQ=255)                                                                                                                                                                            ctTCCAACTTATTCACCCTGTCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACTTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCATCAAAATGCTGTAATTCTccag                                                                            <  2:319235/249‑3 (MQ=255)                                                                                                                                                                              tCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCAGCa                                                                          <  1:60940/249‑1 (MQ=255)                                                                                                                                                                                cAGCTCATTCCCCCTGGCACCGCCCGAGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATTTCAAACGTAGACATCTCTTTGGCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTAGCGCCACCAAAATGCTGTAATGCTCCAGCAATCa                                                                      <  2:654241/251‑1 (MQ=255)                                                                                                                                                                                     cATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCAGCAATCAGCTg                                                                  <  1:217794/250‑1 (MQ=255)                                                                                                                                                                                           cccTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACACTTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCACCACCAAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGTTg                                                           >  2:424272/1‑251 (MQ=255)                                                                                                                                                                                                  aTCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGCAACATCGAGCAAAATGGCCCGATACACTTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGTTTCGGCGAt                                                    >  2:547100/1‑251 (MQ=255)                                                                                                                                                                                                     gACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGtt                                                 <  1:547100/251‑1 (MQ=255)                                                                                                                                                                                                      aCCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATg                                                   <  2:314550/248‑1 (MQ=255)                                                                                                                                                                                                         gcgTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGGTGCTc                                             <  1:108117/251‑1 (MQ=255)                                                                                                                                                                                                             gCAGAAACACCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGGTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCTCCAAAATGCTGTAATTCTCCAGCAATCATCTGCCAGTTGCGGAGATGTTGCTCggg                                          <  2:340001/250‑1 (MQ=255)                                                                                                                                                                                                              cAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGAt                                        <  1:215341/251‑1 (MQ=255)                                                                                                                                                                                                               aGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATg                                       <  1:9616/251‑1 (MQ=255)                                                                                                                                                                                                               aGAAACCCCTGATTATGTTCCTCGTCCATATTCTTCCAGGGGGTACGCATAAATTGTTCCATTAACTGTTGCTCAATCCCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAATCATCGAGCAAAATGTCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATg                                       <  2:562704/251‑1 (MQ=255)                                                                                                                                                                                                                  aaCTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGccc                                    >  1:638239/1‑251 (MQ=255)                                                                                                                                                                                                                   aCTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCt                                   <  1:679355/251‑1 (MQ=255)                                                                                                                                                                                                                     tCCTGATTATGTTCCTCGTCCATATTCTTACAGGTGCTACGCAGACATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCAACCAAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGTTGCCCTTc                                 >  2:388952/1‑251 (MQ=255)                                                                                                                                                                                                                                  ccTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTaaa                     <  1:594745/250‑1 (MQ=255)                                                                                                                                                                                                                                    tCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATa                                                                                                                                             <  1:614756/128‑1 (MQ=255)                                                                                                                                                                                                                                    tCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGACCGATa                                                                                                                                             >  2:614756/1‑128 (MQ=255)                                                                                                                                                                                                                                        ccATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCg              <  1:662177/251‑1 (MQ=255)                                                                                                                                                                                                                                         catatacttcCAGGCGTTACGCAGAAATTGTTCCAGTAACTGTTGATCAATCTCCAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATCCAATTTACCGTGTCCGCGAAGTTTGTTGGCGATACTATCGCCACAAAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGTTGCCCTTCACTCGCTTTAAACATTTCGt             >  2:433074/7‑251 (MQ=255)                                                                                                                                                                                                                                                ttcCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTAt                                                                                                       >  2:532940/1‑154 (MQ=255)                                                                                                                                                                                                                                                ttcCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTAt                                                                                                       <  1:532940/154‑1 (MQ=255)                                                                                                                                                                                                                                                    aGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGTATCAGt  >  1:54947/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       CCCCATTTAGTGCCGCACCTACAGGGCCTCCCAGCCCCGCGCCGCGCAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACACCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATTTTCTTCCAGGTATTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATTTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGCATCAGT  >  NC_000913/10738‑11231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑