Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	10,802	T→C	V185V (GTA→GTG)	yaaW ←	putative enzyme‑specific chaperone YaaW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	10,802	0	T	C	100.0%	121.4 / NA	43	V185V (GTA→GTG)	yaaW	putative enzyme‑specific chaperone YaaW
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/17);  total (26/17)

GGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTA‑GTGCCGCACCTACAGGGCCTCCCAGCCCCGCGCCGCGCAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACACCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATTTTCTTCCAGGTATTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATTTCAAACGTAGACATCTCTTTGTCG  >  NC_000913/10561‑11045                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    gggCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCCAGCACGCTCAtt                                                                                                                                                                                                                                            >  1:175103/1‑251 (MQ=255)               aatCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCg                                                                                                                                                                                                                              >  1:340001/1‑251 (MQ=255)                atCAGCCAGTGGGGCAGTGTCTTCGAGCTTTGGAGGGGAAAATGAAAATTTGCCCCGGGTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTA‑ATGCTGCGCCCACAGTGCTTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACTCTCATTGCTGCGTGGGTGCGt                                                                                                                                                                                                                             <  2:543978/251‑1 (MQ=255)                    gCCAGTGGGGCAGTGTCTACGATCTTGTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGTTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTaaaa                                                                                                                                                                                                                         <  2:479303/251‑1 (MQ=255)                     ccAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAACTTTTCCCCGGTTTACGGTGTCAGCCCTGAGTGGAGCTAACCATCCGGCGCAGGCAGGCGCTTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCACATTTA‑ATGCTGCGCCCACAGGGCATCCCAGACCCCCTCCGCGCAGCAAACCATGCCCAAGCCCGCTCATTGCTACGAGGGGGCGTaatat                                                                                                                                                                                                                        >  2:594745/1‑248 (MQ=255)                       aGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTTCTGCGTGGCGGCGTAAAATGc                                                                                                                                                                                                                      >  2:92522/1‑251 (MQ=255)                                   tCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGGGGGTGCGGAAAATGCGGGTCAATTGGc                                                                                                                                                                                                          >  2:86445/1‑251 (MQ=255)                                        gATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCCCGCTCATTGATGCg                                                                                                                                                                                                                                      >  2:233777/1‑218 (MQ=255)                                        gATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCg                                                                                                                                                                                                                                      <  1:233777/218‑1 (MQ=255)                                                          tgaaaatTTTCCCCGGTTTACGGTGTCCGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGAACGCTTTAACCCCATTTA‑ATGCTGCGCCCCCAGGGCCTCCCCGACCCGAGCCGCGCAGCAAACCAAGCCCCAGCACGCTCATTGCTGCGTGGGt                                                                                                                                                                                                                                 >  2:27616/1‑205 (MQ=255)                                                          tgaaaatTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGt                                                                                                                                                                                                                                 <  1:27616/205‑1 (MQ=255)                                                                       cGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGACAGGCACTGCCGCTGGCCGCTTTAACCCCATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATACCCAAGCACGCCAATTGCCGCGTGGGAGCGGCAACTGCGGGTCAATTGCCTGGAAAGCAAATGCGACACCCCtttacccatttatt                                                                                                                                                                      >  2:580999/1‑240 (MQ=255)                                                                        ggTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGCCACGCCTTTTGCCAATAAttt                                                                                                                                                                     >  1:477370/1‑251 (MQ=255)                                                                                            aGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGgcc                                                                                                                                                 >  1:421635/1‑250 (MQ=255)                                                                                                   cTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCTCATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCAAGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCcgcagcgc                                                                                                                                          >  2:303943/1‑249 (MQ=255)                                                                                                    tAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCAGACCGCTTTAACCCCATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGAGACCCGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGctct                                                                                                                                         >  2:169097/1‑251 (MQ=255)                                                                                                           ccGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCt                                                                                                                                  >  1:562704/1‑251 (MQ=255)                                                                                                             ggCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTc                                                                                                                                 >  1:242494/1‑250 (MQ=255)                                                                                                                           aTTTGAAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCGGACCGCTTTAACCCCATTTA‑ATGCTTCGCCCACCGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAATCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCAt                                                                                                                   <  2:200148/250‑1 (MQ=255)                                                                                                                               caaGTACGGCTGGAAGTGTCACGCGATAGGCACGGCCGCTGACCGCTTTAACCCCATCTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCTCGCTCAGTGCTGCGTGGGTGCGTAAAATGCGTGTCAAGGGGCTGGAAAGCAAATGCGACACGCCGTTTGCCAATAATTTGTCTTTCATCAGCCGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGAATCGAc                                                                                                               <  2:242494/248‑1 (MQ=255)                                                                                                                                      ggCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCa                                                                                                       <  1:93534/251‑1 (MQ=255)                                                                                                                                             attgTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTc                                                                                                >  1:654241/4‑251 (MQ=255)                                                                                                                                                tctccgcgATAGGCACTACCTCTAACCGCTTTAACCACATTTACATCTCGC‑CCTCCAGGGCCGCCCATGCCCGCGCCTCACACAAAACCATGCCCAAGCACGCTCATTGCTGCGTGGTTGCTTAAAATGCGGGTCAATTGGCTTGAAAGCAAATGCGACACGCCTTTTGCCAACTATTTGTCTTCCATCAGCAGCGGCAGCAGCTCGTCCAGCGTAGTCACCCTGGCATCGACCGCGTGCAGAAAcgcca                                                                                              <  2:344785/246‑5 (MQ=255)                                                                                                                                                  cACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGc                                                                                            <  1:92522/250‑1 (MQ=255)                                                                                                                                                      cgATAGGCACTGCCGCTGACCGCTTTAACCCCATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCCGCTCTTCCAGCTCATTCACCATGGCATCGACCGCGTGCAGAAACTCCTGCTTATg                                                                                       >  2:9616/1‑251 (MQ=255)                                                                                                                                                           ggcacTGCCGCTGACCGCTTTAACCCCATTTA‑ATGCTGCGCCCCCAGCGCCTCCCAGCCCCGCGCCGAGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCACTTGGCTGGAAAGCAACTGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTAtactccc                                                                                  >  2:679355/5‑245 (MQ=255)                                                                                                                                                               cTGCCGCTGACCGCTTTAACCCCATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACAATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTACTGCTACTGTTCCTCGTc                                                                              >  2:447729/1‑251 (MQ=255)                                                                                                                                                                 gCCGCTGACCGCCTTAACCCCATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCa                                                                            <  1:17093/251‑1 (MQ=255)                                                                                                                                                                  ccGCTGACCGCTTTAACGCTAATTA‑ATGCGGCGCCCTCAGGGCCTCCCAGACCCGCGCCTCGCAGCAAACCATGCCCAAGCACGCTCATTGCTTCGTGGGTGCGTAAAATGCGGGTCAATGGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCAt                                                                           <  2:489802/251‑1 (MQ=255)                                                                                                                                                                   cGCTGAACGCTTTAACCCCATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCACGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTTCTCGTCCAtt                                                                          >  2:662177/1‑251 (MQ=255)                                                                                                                                                                    gCTGACCGCTTTAACCCCATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGGAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCAtat                                                                         <  1:575848/251‑3 (MQ=255)                                                                                                                                                                             tttAACCCCATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAgg                                                                >  1:278852/1‑251 (MQ=255)                                                                                                                                                                                  ccccATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTa                                                           <  1:594424/251‑1 (MQ=255)                                                                                                                                                                                   cccATTTA‑ATGCTGCGCCCACAGGGCATCCCAGACCCGCGCCGCGCAGCAAACCACGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCCTCGCCCGCGCGCAGACACTCCAGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTAc                                                          >  2:366859/1‑251 (MQ=255)                                                                                                                                                                                                                  cagacccGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCaa                            <  1:589966/247‑1 (MQ=255)                                                                                                                                                                                                                     ccccGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAAtct                         <  1:366859/251‑3 (MQ=255)                                                                                                                                                                                                                         gcgccgcgcAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCaaa                     <  1:86445/251‑1 (MQ=255)                                                                                                                                                                                                                             cgcgcAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGt                  >  1:298110/1‑250 (MQ=255)                                                                                                                                                                                                                                 cagcaAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGCCCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCACATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGCTGCTCAATATCAAACGTAGAc              >  1:66848/1‑250 (MQ=255)                                                                                                                                                                                                                                 cagcaAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGGGTTACGCAGCACTTGTTCCAGTAACTGTTGCTCAATCTCAAACTTAGAc              >  2:342096/1‑250 (MQ=255)                                                                                                                                                                                                                                     aaaCCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGCCCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACAtctc         >  2:264673/1‑251 (MQ=255)                                                                                                                                                                                                                                            gCCCAAGCACGCTCATTGCTGCGTGGGTGCGTCAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGCAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATATCTTTGTCg  >  2:60940/1‑251 (MQ=255)                                                                                                                                                                                                                                            gCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCg  <  2:421635/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    GGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTA‑GTGCCGCACCTACAGGGCCTCCCAGCCCCGCGCCGCGCAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACACCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATTTTCTTCCAGGTATTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATTTCAAACGTAGACATCTCTTTGTCG  >  NC_000913/10561‑11045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑