Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	10,772	C→A	G195G (GGG→GGT)	yaaW ←	putative enzyme‑specific chaperone YaaW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	10,772	0	C	A	97.4%	104.9 / NA	40	G195G (GGG→GGT)	yaaW	putative enzyme‑specific chaperone YaaW
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (25/13);  total (26/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01

GAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTA‑GTGCCGCACCTACAGGGCCTCCCAGCCCCGCGCCGCGCAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACACCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATTTTCTTCCAGGTATTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATTT  >  NC_000913/10527‑11022                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                           gAAATAATTTCCCTGAATAACAGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCAATGCCGCTGACCGCTTTAACCCCATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACccgc                                                                                                                                                                                                                                                       >  1:671098/1‑251 (MQ=255)       atTTCCCTGAATAACAGTAGTGTTTTCAGGGCGCGGCATAATCATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGACTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGATTTAACCCCATTTA‑ATTCTGCGCCCACAGGGCCTCACAGACccgcgccgcg                                                                                                                                                                                                                                                 >  2:594424/1‑251 (MQ=255)                   aacagTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCTCGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCTCGCAGCAAACCAt                                                                                                                                                                                                                                      <  1:544018/246‑1 (MQ=255)                       gTAGTGTTTTCAGGGCGCGGCCTAATAATCAGCCAGTGGGGCAGTGTCTCCGCTCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGAACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCCTTTA‑ATGCTGCGCCCACAGAGCCTCCCAGACCCGACCCGCGCAGCAAACCATGcacc                                                                                                                                                                                                                                 >  2:573100/1‑248 (MQ=255)                       gTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGccc                                                                                                                                                                                                                                  >  1:543978/1‑250 (MQ=255)                       gTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGccc                                                                                                                                                                                                                                  >  1:428882/1‑250 (MQ=255)                                   gggCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCCAGCACGCTCAtt                                                                                                                                                                                                                     >  1:175103/1‑251 (MQ=255)                                                 aatCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCg                                                                                                                                                                                                       >  1:340001/1‑251 (MQ=255)                                                  atCAGCCAGTGGGGCAGTGTCTTCGAGCTTTGGAGGGGAAAATGAAAATTTGCCCCGGGTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTA‑ATGCTGCGCCCACAGTGCTTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACTCTCATTGCTGCGTGGGTGCGt                                                                                                                                                                                                      <  2:543978/251‑1 (MQ=255)                                                    cAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCCCTGCCGCTGACCGCTTTAACCCCATTTA‑TTGCTGCGCACACAGGGCCTCCCAGaccc                                                                                                                                                                                                                                                         >  2:13605/1‑198 (MQ=255)                                                    cAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGaccc                                                                                                                                                                                                                                                         <  1:13605/198‑1 (MQ=255)                                                      gCCAGTGGGGCAGTGTCTACGATCTTGTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGTTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTaaaa                                                                                                                                                                                                  <  2:479303/251‑1 (MQ=255)                                                       ccAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAACTTTTCCCCGGTTTACGGTGTCAGCCCTGAGTGGAGCTAACCATCCGGCGCAGGCAGGCGCTTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCACATTTA‑ATGCTGCGCCCACAGGGCATCCCAGACCCCCTCCGCGCAGCAAACCATGCCCAAGCCCGCTCATTGCTACGAGGGGGCGTaatat                                                                                                                                                                                                 >  2:594745/1‑248 (MQ=255)                                                         aGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTTCTGCGTGGCGGCGTAAAATGc                                                                                                                                                                                               >  2:92522/1‑251 (MQ=255)                                                                     tCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGGGGGTGCGGAAAATGCGGGTCAATTGGc                                                                                                                                                                                   >  2:86445/1‑251 (MQ=255)                                                                          gATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCCCGCTCATTGATGCg                                                                                                                                                                                                               >  2:233777/1‑218 (MQ=255)                                                                          gATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCg                                                                                                                                                                                                               <  1:233777/218‑1 (MQ=255)                                                                                            tgaaaatTTTCCCCGGTTTACGGTGTCCGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGAACGCTTTAACCCCATTTA‑ATGCTGCGCCCCCAGGGCCTCCCCGACCCGAGCCGCGCAGCAAACCAAGCCCCAGCACGCTCATTGCTGCGTGGGt                                                                                                                                                                                                          >  2:27616/1‑205 (MQ=255)                                                                                            tgaaaatTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGt                                                                                                                                                                                                          <  1:27616/205‑1 (MQ=255)                                                                                                         cGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGACAGGCACTGCCGCTGGCCGCTTTAACCCCATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATACCCAAGCACGCCAATTGCCGCGTGGGAGCGGCAACTGCGGGTCAATTGCCTGGAAAGCAAATGCGACACCCCtttacccatttatt                                                                                                                                               >  2:580999/1‑240 (MQ=255)                                                                                                          ggTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGCCACGCCTTTTGCCAATAAttt                                                                                                                                              >  1:477370/1‑251 (MQ=255)                                                                                                                              aGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGgcc                                                                                                                          >  1:421635/1‑250 (MQ=255)                                                                                                                                     cTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCTCATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCAAGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCcgcagcgc                                                                                                                   >  2:303943/1‑249 (MQ=255)                                                                                                                                      tAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCAGACCGCTTTAACCCCATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGAGACCCGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGctct                                                                                                                  >  2:169097/1‑251 (MQ=255)                                                                                                                                             ccGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCt                                                                                                           >  1:562704/1‑251 (MQ=255)                                                                                                                                               ggCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTc                                                                                                          >  1:242494/1‑250 (MQ=255)                                                                                                                                                             aTTTGAAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCGGACCGCTTTAACCCCATTTA‑ATGCTTCGCCCACCGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAATCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCAt                                                                                            <  2:200148/250‑1 (MQ=255)                                                                                                                                                                 caaGTACGGCTGGAAGTGTCACGCGATAGGCACGGCCGCTGACCGCTTTAACCCCATCTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCTCGCTCAGTGCTGCGTGGGTGCGTAAAATGCGTGTCAAGGGGCTGGAAAGCAAATGCGACACGCCGTTTGCCAATAATTTGTCTTTCATCAGCCGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGAATCGAc                                                                                        <  2:242494/248‑1 (MQ=255)                                                                                                                                                                        ggCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCa                                                                                <  1:93534/251‑1 (MQ=255)                                                                                                                                                                               attgTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTc                                                                         >  1:654241/4‑251 (MQ=255)                                                                                                                                                                                  tctccgcgATAGGCACTACCTCTAACCGCTTTAACCACATTTACATCTCGC‑CCTCCAGGGCCGCCCATGCCCGCGCCTCACACAAAACCATGCCCAAGCACGCTCATTGCTGCGTGGTTGCTTAAAATGCGGGTCAATTGGCTTGAAAGCAAATGCGACACGCCTTTTGCCAACTATTTGTCTTCCATCAGCAGCGGCAGCAGCTCGTCCAGCGTAGTCACCCTGGCATCGACCGCGTGCAGAAAcgcca                                                                       <  2:344785/246‑5 (MQ=255)                                                                                                                                                                                    cACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGc                                                                     <  1:92522/250‑1 (MQ=255)                                                                                                                                                                                        cgATAGGCACTGCCGCTGACCGCTTTAACCCCATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCCGCTCTTCCAGCTCATTCACCATGGCATCGACCGCGTGCAGAAACTCCTGCTTATg                                                                >  2:9616/1‑251 (MQ=255)                                                                                                                                                                                             ggcacTGCCGCTGACCGCTTTAACCCCATTTA‑ATGCTGCGCCCCCAGCGCCTCCCAGCCCCGCGCCGAGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCACTTGGCTGGAAAGCAACTGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTAtactccc                                                           >  2:679355/5‑245 (MQ=255)                                                                                                                                                                                                 cTGCCGCTGACCGCTTTAACCCCATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACAATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTACTGCTACTGTTCCTCGTc                                                       >  2:447729/1‑251 (MQ=255)                                                                                                                                                                                                   gCCGCTGACCGCCTTAACCCCATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCa                                                     <  1:17093/251‑1 (MQ=255)                                                                                                                                                                                                    ccGCTGACCGCTTTAACGCTAATTA‑ATGCGGCGCCCTCAGGGCCTCCCAGACCCGCGCCTCGCAGCAAACCATGCCCAAGCACGCTCATTGCTTCGTGGGTGCGTAAAATGCGGGTCAATGGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCAt                                                    <  2:489802/251‑1 (MQ=255)                                                                                                                                                                                                     cGCTGAACGCTTTAACCCCATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCACGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTTCTCGTCCAtt                                                   >  2:662177/1‑251 (MQ=255)                                                                                                                                                                                                      gCTGACCGCTTTAACCCCATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGGAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCAtat                                                  <  1:575848/251‑3 (MQ=255)                                                                                                                                                                                                               tttAACCCCATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAgg                                         >  1:278852/1‑251 (MQ=255)                                                                                                                                                                                                                    ccccATTTA‑ATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTa                                    <  1:594424/251‑1 (MQ=255)                                                                                                                                                                                                                     cccATTTA‑ATGCTGCGCCCACAGGGCATCCCAGACCCGCGCCGCGCAGCAAACCACGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCCTCGCCCGCGCGCAGACACTCCAGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTAc                                   >  2:366859/1‑251 (MQ=255)                                                                                                                                                                                                                                                       ccccGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAAtct  <  1:366859/251‑3 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                           GAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTA‑GTGCCGCACCTACAGGGCCTCCCAGCCCCGCGCCGCGCAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACACCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATTTTCTTCCAGGTATTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATTT  >  NC_000913/10527‑11022

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑