Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	10,642	A→G	intergenic (‑148/+1)	satP ← / ← yaaW	acetate/succinate:H(+) symporter/putative enzyme‑specific chaperone YaaW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	10,642	0	A	G	93.5%	127.8 / 0.2	46	intergenic (‑148/+1)	satP/yaaW	acetate/succinate:H(+) symporter/putative enzyme‑specific chaperone YaaW
Reads supporting (aligned to +/- strand):  ref base A (1/2);  new base G (29/14);  total (30/16)
Fisher's exact test for biased strand distribution p-value = 2.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.16e-01

CGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCACCTACAGGGCCTCCCAGCCCCGCGCCGCGCAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACACCTTTTGCCAATAATTT  >  NC_000913/10410‑10882                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                 cGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACAGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCt                                                                                                                                                                                                                                >  1:479303/1‑251 (MQ=255)          gcgtaaacagaatgttgaacATGCCGCAGACCCTCACGCCCAGCGGAGCCAGATTAGCCAACTTATTGTTGCCCATAATTACTCAACAATCATCATCGAATGAACGGTGAAATAATTTCCCTGAATAAAAGTAGTGTTTTCAGGGCGCGGCATAATACTCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGTGTAAAATGAAAATTTTCCCCGGTTTACGGTGACAGAAATGAGTGGCGGTAACCATcat                                                                                                                                                                                                                       >  2:108117/20‑249 (MQ=255)             tAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACAGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGgcg                                                                                                                                                                                                                    >  1:351970/1‑251 (MQ=255)                  aGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCgcaggc                                                                                                                                                                                                               >  1:288273/1‑251 (MQ=255)                  aGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACAGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCGGACCTGGGTGGCGCTAACCATCCGGCgcaggc                                                                                                                                                                                                               >  2:202153/1‑251 (MQ=255)                         tggtCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACAGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGACGGCAGGCggt                                                                                                                                                                                                        >  2:93534/1‑249 (MQ=255)                                  cGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACAGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTAc                                                                                                                                                                                               >  1:337320/1‑251 (MQ=255)                                    aaGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACAGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACgg                                                                                                                                                                                             >  1:319235/1‑251 (MQ=255)                                           tCAGGCCCAGCGGTGCAGGATTAGCCAACTTAGCGTTGCCCCTAATGCCTCAACAAACATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACAGTAGTGTTTTCACGGCGCGGCATAATAATCTGCCAGTGGGGCAGTGTCTACGCTCTTTAGTGGGGAAAATGCAAATTTTCCCCGGTTTATGGTGTCTGACCTGCGTGTCGCTACACATCCGGCGCAGGCAGGCGATTGGCAGTAAGGcgggcat                                                                                                                                                                                      >  2:29909/1‑245 (MQ=255)                                                 ccAGCGGTGCCGGATTAGCCAATTTAGTGTTGCCCATAATTCCTCAAAAATCATCAACGAATGAATGGTGAAATAATTTCCCTGAATAACAGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCAc                                                                                                                                                                                <  2:529585/251‑1 (MQ=255)                                                  cAGCGGTGCCGGAATAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGAGAAATAATTTCCCTGAATAACAGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCAcg                                                                                                                                                                               <  2:40821/251‑1 (MQ=255)                                                   aGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACAGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCAcgc                                                                                                                                                                              >  1:467283/1‑251 (MQ=255)                                                        tgccgaaatAGCCAACTTAGAGTTGACCATAATTACTCAAAAATAATCATCGAATGAATGGTGAAATAATTTCCCTGAGTAACAGTCGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGAGAAAATGAAAATTTTCCCAGGTTTAAGGTGTCAGACCTGAGTGGAGCTACCAATCAGGCGCAGGCCGTAGATTTGCAGTTCGGCGGGAATTGACACGCGAt                                                                                                                                                                           >  2:622933/9‑249 (MQ=255)                                                                    cAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACAGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTg                                                                                                                                                             <  1:522076/251‑1 (MQ=255)                                                                         tAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACAGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACTGCTGTAATTGTCACGCGATAGGCACTGCCGCTGACCg                                                                                                                                                         <  1:211911/250‑1 (MQ=255)                                                                         tAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACAGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCg                                                                                                                                                         <  1:509807/250‑1 (MQ=255)                                                                                       aTTCCGCAAAAATCATCATCGAAGGAATGGTGAAATAATTTCCCTGAATAACAGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTa                                                                                                                                          <  2:342067/251‑1 (MQ=255)                                                                                             cAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGc                                                                                                                                      <  2:288273/249‑1 (MQ=255)                                                                                             cAAAAAGCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACAGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTAATGctg                                                                                                                                    <  1:238047/251‑3 (MQ=255)                                                                                               aaaaTCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACAGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTAATGctgcg                                                                                                                                  >  1:611597/1‑247 (MQ=255)                                                                                                    catcatcGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCACCTAc                                                                                                                             <  1:307868/251‑1 (MQ=255)                                                                                                     atcatcGAATGAATGGTGAAATAATTTCCCTGAATAACAGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTAATGctgcgcccac                                                                                                                             >  1:400146/1‑241 (MQ=255)                                                                                                                      gAAATAATTTCCCTGAATAACAGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCAATGCCGCTGACCGCTTTAACCCCATTTAATGCTGCGCCCACAGGGCCTCCCAGACccgc                                                                                                           >  1:671098/1‑251 (MQ=255)                                                                                                                            atTTCCCTGAATAACAGTAGTGTTTTCAGGGCGCGGCATAATCATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGACTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGATTTAACCCCATTTAATTCTGCGCCCACAGGGCCTCACAGACccgcgccgcg                                                                                                     >  2:594424/1‑251 (MQ=255)                                                                                                                               tCCCTGAATAACAGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCa                                                                                                                                                                                                   >  1:208618/1‑154 (MQ=255)                                                                                                                               tCCCTGAATAACAGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCa                                                                                                                                                                                                   <  2:208618/154‑1 (MQ=255)                                                                                                                                        aacagTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCTCGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTAATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCTCGCAGCAAACCAt                                                                                          <  1:544018/246‑1 (MQ=255)                                                                                                                                            gTAGTGTTTTCAGGGCGCGGCCTAATAATCAGCCAGTGGGGCAGTGTCTCCGCTCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGAACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCCTTTAATGCTGCGCCCACAGAGCCTCCCAGACCCGACCCGCGCAGCAAACCATGcacc                                                                                     >  2:573100/1‑248 (MQ=255)                                                                                                                                            gTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTAATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGccc                                                                                      >  1:543978/1‑250 (MQ=255)                                                                                                                                            gTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTAATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGccc                                                                                      >  1:428882/1‑250 (MQ=255)                                                                                                                                                        gggCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTAATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCCAGCACGCTCAtt                                                                         >  1:175103/1‑251 (MQ=255)                                                                                                                                                                      aatCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTAATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCg                                                           >  1:340001/1‑251 (MQ=255)                                                                                                                                                                       atCAGCCAGTGGGGCAGTGTCTTCGAGCTTTGGAGGGGAAAATGAAAATTTGCCCCGGGTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTAATGCTGCGCCCACAGTGCTTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACTCTCATTGCTGCGTGGGTGCGt                                                          <  2:543978/251‑1 (MQ=255)                                                                                                                                                                         cAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCCCTGCCGCTGACCGCTTTAACCCCATTTATTGCTGCGCACACAGGGCCTCCCAGaccc                                                                                                             >  2:13605/1‑198 (MQ=255)                                                                                                                                                                         cAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTAATGCTGCGCCCACAGGGCCTCCCAGaccc                                                                                                             <  1:13605/198‑1 (MQ=255)                                                                                                                                                                           gCCAGTGGGGCAGTGTCTACGATCTTGTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGTTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTAATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTaaaa                                                      <  2:479303/251‑1 (MQ=255)                                                                                                                                                                            ccAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAACTTTTCCCCGGTTTACGGTGTCAGCCCTGAGTGGAGCTAACCATCCGGCGCAGGCAGGCGCTTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCACATTTAATGCTGCGCCCACAGGGCATCCCAGACCCCCTCCGCGCAGCAAACCATGCCCAAGCCCGCTCATTGCTACGAGGGGGCGTaatat                                                     >  2:594745/1‑248 (MQ=255)                                                                                                                                                                              aGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTAATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTTCTGCGTGGCGGCGTAAAATGc                                                   >  2:92522/1‑251 (MQ=255)                                                                                                                                                                                          tCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTAATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGGGGGTGCGGAAAATGCGGGTCAATTGGc                                       >  2:86445/1‑251 (MQ=255)                                                                                                                                                                                               gATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTAATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCCCGCTCATTGATGCg                                                                   >  2:233777/1‑218 (MQ=255)                                                                                                                                                                                               gATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTAATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCg                                                                   <  1:233777/218‑1 (MQ=255)                                                                                                                                                                                                                 tgaaaatTTTCCCCGGTTTACGGTGTCCGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGAACGCTTTAACCCCATTTAATGCTGCGCCCCCAGGGCCTCCCCGACCCGAGCCGCGCAGCAAACCAAGCCCCAGCACGCTCATTGCTGCGTGGGt                                                              >  2:27616/1‑205 (MQ=255)                                                                                                                                                                                                                 tgaaaatTTTCCCCGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTAATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGt                                                              <  1:27616/205‑1 (MQ=255)                                                                                                                                                                                                                           ccccGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTAATGCTGCGCCCACAGGGCCt                                                                                                                      >  1:378857/1‑139 (MQ=255)                                                                                                                                                                                                                              cGGTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGACAGGCACTGCCGCTGGCCGCTTTAACCCCATTTAATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATACCCAAGCACGCCAATTGCCGCGTGGGAGCGGCAACTGCGGGTCAATTGCCTGGAAAGCAAATGCGACACCCCtttacccatttatt   >  2:580999/1‑240 (MQ=255)                                                                                                                                                                                                                               ggTTTACGGTGTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCACGCGATAGGCACTGCCGCTGACCGCTTTAACCCCATTTAATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGCCACGCCTTTTGCCAATAAttt  >  1:477370/1‑251 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                 CGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCACCTACAGGGCCTCCCAGCCCCGCGCCGCGCAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACACCTTTTGCCAATAATTT  >  NC_000913/10410‑10882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑