Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 10,260 G→A L79L (CTG→TTG)  satP ← acetate/succinate:H(+) symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091310,2600GA95.8% 134.0 / ‑4.4 51L79L (CTG→TTG) satPacetate/succinate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (22/24);  total (24/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

GCAGATCAGCCCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACGGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCA‑ACCAGCGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCC  >  NC_000913/10015‑10500
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                
acAGATCAGACCAAGCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCA‑ACCAACGTCa                                                                                                                                                                                                                                             <  1:175496/250‑1 (MQ=255)
         accAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCA‑ACCAACGTCAGCCagaa                                                                                                                                                                                                                                      >  1:191910/2‑249 (MQ=255)
            aaTCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAACCGCA‑ACCAACGTCCGCCAGAAAGAAc                                                                                                                                                                                                                                 >  2:423127/1‑251 (MQ=255)
             gtCCAGCCGGAAAAGTGGATGATTGCGGAGTTACCGGCAACGTTACCGAACGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAAAGCGTGCGCATTTAAGCGTGCCGAAGAACATAAACAGCGTAAATACGCACCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATAGGACAACCCCAGTTTCGACATCAGCAGAATCGCC‑ACAAACGTCGGCCAGAAAGAAcc                                                                                                                                                                                                                                >  2:522076/2‑251 (MQ=255)
                  gCCGGCAAAGTGGATGCTTGCGGCGTTACCGGCAATGTTCCCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCA‑ACCAACGTCAGCCAGAAAGAACCGTa                                                                                                                                                                                                                             >  1:565609/1‑249 (MQ=255)
                    cGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCA‑ACCAACGTCAGCCAGAAAGAACCGTAagag                                                                                                                                                                                                                         <  2:108100/251‑1 (MQ=255)
                     ggCAAAGTGGATGATTGCGGCGTTACCGGCAAAGTTACCGCTCGCCAGCAGGGCAAACAGCACAGTCAGGCTCCAGAAAACGAATTGAAGAACGCGTGCGCCTTTCAGAGTGCCGAAGAACCTAAACAGCGTAAATCCGACCCACAGACCCAGGTAGACACAAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATAGAA‑AACAACGTCAGCCAGACAGAACCgacagagg                                                                                                                                                                                                                        >  1:27164/1‑244 (MQ=255)
                               atgatTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACATCCCAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGTTAGACTACAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCA‑ACCAACGTCAGCCAGAAAGAACCGTAATAGGTGATTGCGGt                                                                                                                                                                                                              <  2:312248/251‑1 (MQ=255)
                                tgatTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCA‑ACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAAAGCGGtt                                                                                                                                                                                                             >  2:243059/1‑251 (MQ=255)
                                       ggcgTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCA‑ACCAACGTCAGCCAGAAAGCACCGTAAGAGGTGAATGCGGTTaa                                                                                                                                                                                                           >  2:199217/1‑246 (MQ=255)
                                       ggcgTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCA‑ACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTaa                                                                                                                                                                                                           <  1:199217/246‑1 (MQ=255)
                                                      gTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCA‑ACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCttttt                                                                                                                                                                                       <  2:386344/251‑1 (MQ=255)
                                                         aCCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGTCCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCAACGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCA‑ACCAACGGCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAATGTGTTGCCTTTTTTg                                                                                                                                                                                     <  2:581663/250‑1 (MQ=255)
                                                          ccGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCA‑ACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGt                                                                                                                                                                                    >  1:447557/1‑250 (MQ=255)
                                                            gATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCA‑ACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACt                                                                                                                                                                                 <  1:423127/251‑1 (MQ=255)
                                                                   agccgtgaaaaCCGCACAGTCAGGCTAAAGAAAACGCATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGACGAACATAAACAGCGTAACTACGCCACACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCGGTTTCGCCATCAGCATCATCGCACACCA‑CGTCATACATAAAGAAACGTACGAGGTAAATGCGATTAAACCGAAAGAGTTTCCTTTTTTGtcatacagc                                                                                                                                                                            >  2:300041/9‑241 (MQ=255)
                                                                   agcagGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTTCGCCTTACAGCGTGCCGAAGAACATAAACAGAGTAAATACACCCCCCAGACTCAGGTAGACAACAAGGAACTGTGCATTTGGCGCATCGGCCAGACCCAGTTTGGGAATCAGCAGAATAGCA‑ACCAACGTCAGCCAGAAAGAACCGAAAGAGAAGAATGCGGTTAAACCGAAAGCGTAGACTTTTTTGTACTCcagc                                                                                                                                                                            >  1:429258/1‑249 (MQ=255)
                                                                       gggCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCA‑ACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGAccg                                                                                                                                                                      >  1:368142/1‑250 (MQ=255)
                                                                               aGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCA‑ACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAAt                                                                                                                                                              >  1:264176/1‑251 (MQ=255)
                                                                                        aGGCTAAAGAAAACGAAATGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCA‑ACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCTGCAGACCGGCAAAAATTTGCGCGAt                                                                                                                                                     <  2:565609/251‑1 (MQ=255)
                                                                                              aaGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCA‑ACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATgccgcc                                                                                                                                               >  1:232568/1‑251 (MQ=255)
                                                                                              aaGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCA‑ACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGGACTCCAGCAGACCGGCAAAAATTTGCGCGATgccgcc                                                                                                                                               <  1:288299/251‑1 (MQ=255)
                                                                                                 aaaaCGAATTGCAGAACGCGTGCGCCGTTCAGCGTGCCAAAGAACATAAACCGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCA‑ACCAACGTCAGCCAGAAAGAACCGTAAGAGGTTAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTTCAGCAGACCGGCAAAAATTTGCGCGATGCTGCCGTa                                                                                                                                            <  2:653212/251‑1 (MQ=255)
                                                                                                  aaaCGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCA‑ACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGACTGCGGTTAAACCGAAAGTGTTGCCTTTTTTGGACTCAAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTa                                                                                                                                            >  1:202593/1‑250 (MQ=255)
                                                                                                     cGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCA‑ACCAACGTCAGcc                                                                                                                                                                                                                                          <  1:245261/153‑1 (MQ=255)
                                                                                                     cGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCA‑ACCAACGTCAGcc                                                                                                                                                                                                                                          >  2:245261/1‑153 (MQ=255)
                                                                                                       aaTTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCA‑ACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAAt                                                                                                                                      <  1:38884/251‑1 (MQ=255)
                                                                                                             aGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCA‑ACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGCTGCCGCCGTAGAAAATGCCCAt                                                                                                                                >  1:43913/1‑251 (MQ=255)
                                                                                                               aaCGCGTGCGCCTTTCAGCGTGCCGAAGAACATATACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCA‑ACCAGCGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCATTTTTTGTACTCAAGCAGACCAGCAAAACTTTGCGCGATGCCGCCGTAGAAAATGCCCCTgg                                                                                                                              >  2:307868/1‑251 (MQ=255)
                                                                                                               aaCGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCA‑ACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATgg                                                                                                                              >  1:40821/1‑251 (MQ=255)
                                                                                                                   cgTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCA‑ACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCa                                                                                                                            >  2:132254/1‑249 (MQ=255)
                                                                                                                    gTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCCTCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCA‑ACCAACGTCAGCCAGAAAGAACCGTAAGAGGAGCATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCa                                                                                                                            >  1:650331/1‑248 (MQ=255)
                                                                                                                      gcgcCATTCAGCGTGCCGAAGAACATAAACAGCGTAAATACTCCCCACAGACCCAGGTATACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCAAGTTTCGGTATCAGCAGAATCGCA‑ACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGaat                                                                                                                       <  2:191910/251‑1 (MQ=255)
                                                                                                                          cTTTCAGCGTGCAGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCA‑ACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAACAATTTGCGCGATGCCGCCGTATAAAATGCCCATGGCAAGaataat                                                                                                                    >  1:125194/1‑250 (MQ=255)
                                                                                                                             tCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCA‑ACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAATTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATAcc                                                                                                                 <  1:637237/250‑1 (MQ=255)
                                                                                                                                gCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCAAAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCA‑ACCAACGGCAGCTAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTcc                                                                                                             <  1:622368/251‑1 (MQ=255)
                                                                                                                                 cGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCA‑ACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCa                                                                                                            <  1:132254/251‑1 (MQ=255)
                                                                                                                                            acaaaaacgacGTAAATCCGCCCCACAGCCCCAGGTAGACACCAAGGAACTTCGCATTTGGCGCATCTGTCAGACCCAGTTTCGGCAACAGCAGAATCGCA‑ACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAAGGCGGTAAAACCGAAAGTGTTGCCTTTGTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGAGGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATaa                                                                                                 <  2:396684/241‑1 (MQ=255)
                                                                                                                                                      cGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCAGCGGTCAGACCCAGTTGCGGCAACAGCAGAATCGCA‑ACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTAAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTtgt                                                                                       <  2:264176/251‑1 (MQ=255)
                                                                                                                                                                ccccACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCCGCAGAATCGCA‑ACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGGCCAGAGCGAAATAACCCCCGTTGTGCAGGTTa                                                                               >  2:509807/1‑249 (MQ=255)
                                                                                                                                                                    acaGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCA‑ACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCt                                                                                                                                                                                           <  1:448292/137‑1 (MQ=255)
                                                                                                                                                                    acaGACCCAGGTAGACACCAAGGAACTGTGCATTAGGCGAATCGGTCAGACCCCGTTTCGGCATCAGCAGAATCGCA‑ACCAACGTCAGCCAGAAAGAACCGTACGAGGAGAATGCGGTTAAACCGAAAGTGTTGCCt                                                                                                                                                                                           >  2:448292/1‑137 (MQ=255)
                                                                                                                                                                                aGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGAATCAGCAGAATCGCA‑ACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATg                                                             <  2:207852/251‑1 (MQ=255)
                                                                                                                                                                                      cAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCA‑ACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGCCCAGAGCGAAATAACCCACGTTGTGCCGGTTAAGCAGAATGGTGGTCATGCCGaa                                                        >  2:119463/1‑250 (MQ=255)
                                                                                                                                                                                                         cgcATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCA‑ACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTg                                    <  1:638544/251‑1 (MQ=255)
                                                                                                                                                                                                                gTCAGACCCAGTTTCGGCATCAGCAGAATCGCA‑ACCACCGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATTCCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGAtt                             <  1:623128/251‑1 (MQ=255)
                                                                                                                                                                                                                 ccAGACCCAGTTTCGGCAACAGCATAATGGAA‑ACCACCGTAAGCCAGAAAGACCCGTAAGAGGTAAATGCGGTTAAACCGAAAGTGTGGCTTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATATCCCACGTTGTGCAGGTTAAGCAGAATGGTGTTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTa                            <  2:447557/250‑1 (MQ=255)
                                                                                                                                                                                                                     aCCCAGTTTCGGCAACAGCAAAATCGCA‑ACCAACGTCAGCCACAAAGAACCGGAAGAGGAGAATGGGGTTAAACCGAAAGTGTTGCCTTTGTAGTACGCCAGCAGACCGACAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGGCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGTCCAGCGGTGCCGGATTAGCCa                        <  2:110624/251‑1 (MQ=255)
                                                                                                                                                                                                                             ccggtaccagcagAATCGCA‑ACCAACGACAGCCATAAAGACCCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGGTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAgtg                <  2:43913/244‑1 (MQ=255)
                                                                                                                                                                                                                                gCATCAGTAGAATCGCA‑ACCAGCCTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGCGTTGCCTTTTTTGTACCCCAGCAGACCGGCAAAAATTTGCGCGATGCAGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCGCGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGGGTTg             <  2:48425/251‑1 (MQ=255)
                                                                                                                                                                                                                                           aTCGCA‑ACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAATTGTTTCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTcc  <  2:179995/251‑1 (MQ=255)
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GCAGATCAGCCCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACGGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCA‑ACCAGCGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCC  >  NC_000913/10015‑10500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑