Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	8,996	G→T	A253A (GCG→GCT)	talB →	transaldolase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	8,996	0	G	T	96.5%	179.0 / ‑0.5	58	A253A (GCG→GCT)	talB	transaldolase B
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (22/34);  total (23/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.30e-01

CGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTG‑ACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGC  >  NC_000913/8764‑9239                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                    cGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAAGTCTGGAACTGGCAGGCTGCGACCGTCTT‑ACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGc                                                                                                                                                                                                                                       <  1:441323/247‑1 (MQ=255)        tgttgGCCGTATTCTTGGCTAGTCCAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGAAGAAGATCCAGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACACAGAGCACGGTTATGAACCCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAATTTCTGGAACTGACAGGCGGAGGCCGTCAT‑ACCATCGCACCTTAACTTCTGAAAGAGCTGGATGAGGACGCAGGGGCTATctcacgt                                                                                                                                                                                                                             >  2:69604/1‑244 (MQ=255)          ttgGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTT‑ACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTaa                                                                                                                                                                                                                           >  1:334059/1‑250 (MQ=255)              ccGTATTCTTGACTGGTACAAAGCGAAGACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTT‑ACCATCGCCCCTACTCTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGt                                                                                                                                                                                                                      <  1:491214/251‑1 (MQ=255)               cGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTT‑ACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTc                                                                                                                                                                                                                     >  2:245930/1‑251 (MQ=255)                gttttCTTGACTGCTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATGCTGGAACTGGCAGGCTGCGACCGTCTT‑ACCATCGCACCTACACTGCTGAAAGAGCTGGTTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCt                                                                                                                                                                                                                    <  2:640292/248‑1 (MQ=255)                  aTTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTT‑ACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCtt                                                                                                                                                                                                                   <  1:129773/250‑1 (MQ=255)                  aTTCGTGACTGGTACAAAGCTAATACCGATACGAAAGAGTACGATACTGCAGAAGATCCGGGCGTGTTTTCTGTATCTGAAATCTACCAGTACTAGACAGAGCACGGTTATGAAACCGTGGTTAGGGGCGCAAGCTTCCGTTACATCGGCGTATTTCTTGAACTGGCAGGCTGCGACCGTCTT‑ACCATCGCACATACACTGCTGAAATAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCtt                                                                                                                                                                                                                   <  2:409709/250‑1 (MQ=255)                     cTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTT‑ACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAAAGCGAAGGGGCTCTCGAACGTAAACTGACTTacac                                                                                                                                                                                                               >  2:78522/1‑251 (MQ=255)                       tGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTT‑ACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTaca                                                                                                                                                                                                                <  1:617074/248‑1 (MQ=255)                       tGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTT‑ACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACAcc                                                                                                                                                                                                              <  1:619031/250‑1 (MQ=255)                          cTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTT‑ACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCg                                                                                                                                                                                                          >  1:513840/1‑251 (MQ=255)                          cTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTACGGCAGAAGGTCCGGGCGTGGTTTCTGTATCTAAAATCTGCCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTAAGGGCGCAAGCTTCAGTAACATCGGCGAAATTCTGGGACTGGCAGGCTGCGACCGTCTT‑ACCATCGCACCGACACTGCTGAAAGAGCTGGCTTAGATCGAAGGGGCTATCGAACGTAAACTTCCTTACACCGGCg                                                                                                                                                                                                          >  1:302319/1‑251 (MQ=255)                                 aaaGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTT‑ACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGaa                                                                                                                                                                                                   <  1:552061/251‑1 (MQ=255)                                    gCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTT‑ACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAACCTGTCTTACACCGGCGAAGTGAAAgc                                                                                                                                                                                                >  1:449963/1‑251 (MQ=255)                                       aaTACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTAGCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTT‑ACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGcgcg                                                                                                                                                                                             <  2:669653/251‑1 (MQ=255)                                               tAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTT‑ACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGgcgc                                                                                                                                                                                     >  2:592902/1‑251 (MQ=255)                                                        gTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTT‑ACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTg                                                                                                                                                                            >  1:194028/1‑251 (MQ=255)                                                          ccGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACCAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTT‑ACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAg                                                                                                                                                                          <  2:194028/250‑1 (MQ=255)                                                                cGGCCGAAGAGCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTGATTGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTT‑ACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAACCTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAg                                                                                                                                                                    <  2:264019/251‑1 (MQ=255)                                                                 ggCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTT‑ACCATCGCACCTACACTGCTGAAAGAGCTGGCTGCGAGCGCAGGGGCTATCGAACGAAAACTGTCTTACACCGGCGAAGAGAAAGCGCGTCCGGCGCGTCTCACTGAGTCCGAGt                                                                                                                                                                   >  2:175862/1‑251 (MQ=255)                                                                 ggCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTT‑ACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGCGTCCGAGt                                                                                                                                                                   >  1:5365/1‑251 (MQ=255)                                                                   cAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTT‑ACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTACACTGTCTTACACCGGCGAAGGGAAAGCGCGTCCGGCGCGTAACACTGAGTCCGGGTTc                                                                                                                                                                 >  2:674092/1‑251 (MQ=255)                                                                                 gTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTT‑ACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGcaca                                                                                                                                                    >  1:625837/1‑250 (MQ=255)                                                                                    gTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTT‑ACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCa                                                                                                                                                <  1:492517/251‑1 (MQ=255)                                                                                       tCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTG‑ACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGcgcg                                                                                                                                                                                    >  2:251238/1‑212 (MQ=255)                                                                                       tCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTG‑ACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGcgcg                                                                                                                                                                                    <  1:251238/212‑1 (MQ=255)                                                                                          gTATCTGAAATCTACCAGTACTACAAAGAGCATGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTT‑ACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGAt                                                                                                                                            <  1:515846/249‑1 (MQ=255)                                                                                          gTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTT‑ACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGa                                                                                                                                             <  1:626036/248‑1 (MQ=255)                                                                                           tATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTT‑ACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGAGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATcc                                                                                                                                          >  1:554604/1‑250 (MQ=255)                                                                                              ttGAAATCTCCCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATGCTGGAACTGGCAGGCTGCGACCGTCTT‑ACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAAt                                                                                                                                       <  2:486648/249‑1 (MQ=255)                                                                                                 aaaTCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTT‑ACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGGCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCa                                                                                                                                   <  1:640019/251‑1 (MQ=255)                                                                                                                acAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTT‑ACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCg                                                                                                                    <  1:314753/251‑1 (MQ=255)                                                                                                                   aaGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATAGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTT‑ACCATCGAACCTACACTGCTGAAAGAGCTGACTGAGAGCGACGGGGCTAACGAACGTAAACAGTCTTAAACCGGCGAAGTGAACGCGCGTCCGGCGCGTATCACTGCGTCCGAATTCCTGTGGCAGCCCAACCAGGATCAAATGGCCGTAGGTAAACTGGCGGa                                                                                                                  >  2:346714/1‑250 (MQ=255)                                                                                                                        cACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTT‑ACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTAt                                                                                                            <  1:78522/251‑1 (MQ=255)                                                                                                                             ttATGAATCCGTGGTTATGGGCTCACGTGTCCGGCACATCGTCGAACTTCTGGAATTGTCAGTCTGTGACCGT‑TGTACCATCGCATCTACACTGCTGAACTGGCTGGCTGAGAGCAAAGGGGCTATCGCACTTAAGCTGTCTTACATCGGCGAAGTGAAAGCGCGTCCGGCGCTTACCTCTGAGGCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATccta                                                                                                        <  2:467216/250‑3 (MQ=255)                                                                                                                               aTGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTT‑ACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTaa                                                                                                      <  1:516890/250‑1 (MQ=255)                                                                                                                                            tattggCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTT‑ACCATCGCACCTACACTGCTGAAAGAGCTGGGTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGAc                                                                                         <  2:109445/246‑1 (MQ=255)                                                                                                                                               gggCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTT‑ACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAgg                                                                                     <  1:592902/251‑1 (MQ=255)                                                                                                                                                 gcgcAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTT‑ACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGa                                                                                    >  1:314384/1‑250 (MQ=255)                                                                                                                                                   gcAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTT‑ACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGaaaa                                                                                 <  1:26594/251‑1 (MQ=255)                                                                                                                                                   gcAAGCTTCCGTAACACCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTT‑ACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGaaaa                                                                                 <  2:113579/251‑1 (MQ=255)                                                                                                                                                               aaCATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTT‑ACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGaaaaaa                                                                      <  1:166882/250‑1 (MQ=255)                                                                                                                                                               aaCATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTT‑ACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAAt                                                                     <  1:346714/251‑1 (MQ=255)                                                                                                                                                                aCATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTT‑ACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGTTAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATg                                                                    <  2:625837/251‑1 (MQ=255)                                                                                                                                                                 cATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTT‑ACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGa                                                                   <  1:368330/251‑1 (MQ=255)                                                                                                                                                                         aaaTTCTGGAACTGGCAGGCTGCGACCGTCTT‑ACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGAt                                                           >  1:477385/1‑251 (MQ=255)                                                                                                                                                                           aTTCTGGAACTGGCAGGCTGCGACCGTCTT‑ACCATCGCACCTACACTGCTGAAATAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATct                                                         <  2:291601/251‑1 (MQ=255)                                                                                                                                                                            ttCTGGAACTGGCAGGCTGCGACCGTCTT‑ACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACAGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGCTAAACTGGCGGAAGGTATCCGTAAGGTTGCTATTGACCAGGAAAAACTGGGAAAAATGATCGGCGATctg                                                        >  1:27872/1‑251 (MQ=255)                                                                                                                                                                                         aGGCTGCGACCGTCTT‑ACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGcaca                                                                                                                                                    <  1:447661/146‑1 (MQ=255)                                                                                                                                                                                         aGGCTGCGACCGTAAT‑ACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACAGAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGcaca                                                                                                                                                    >  2:447661/1‑146 (MQ=255)                                                                                                                                                                                                   cGTCTT‑ACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGAcc                                 <  1:245930/251‑1 (MQ=255)                                                                                                                                                                                                    gTCTT‑ACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCCATGGCAGTAGATaa                                                                                                                           >  2:608804/1‑160 (MQ=255)                                                                                                                                                                                                    gTCTT‑ACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATaa                                                                                                                           <  1:608804/160‑1 (MQ=255)                                                                                                                                                                                                            cATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTATTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCGGCTGTAATCATTCTTAGCGTGACCGGGAAGTc                         <  2:314384/251‑1 (MQ=255)                                                                                                                                                                                                                              gAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCtcttc        <  1:50970/250‑1 (MQ=255)                                                                                                                                                                                                                                 cgagCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTCCACCGGCGGAGTGAAACCGCGCCCGGCGCGGATCACATCGTCCGCGTTCCCGTGGCAGCACAACCAGGATCCAATAGCAGTAGATAAACTGGCGGCAGCTATGCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAACTGATCGGCGATCTGCTGTAATCATTTTTAGGGTAACCGAGAAGTCGGACAAGCTACctctcctaa     >  1:380229/2‑245 (MQ=255)                                                                                                                                                                                                                                   agCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGc  >  1:552968/1‑251 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                    CGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTG‑ACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGC  >  NC_000913/8764‑9239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑