| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 7,939 | G→A | F7F (TTC→TTT) | yaaJ ← | putative transporter YaaJ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 7,939 | 0 | G | A | 89.8% | 141.1 / 7.0 | 59 | F7F (TTC→TTT) | yaaJ | putative transporter YaaJ |
| Reads supporting (aligned to +/- strand): ref base G (4/2); new base A (23/30); total (27/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.98e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.05e-01 | |||||||||||
GTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGAT‑GTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTT‑ATTGCCGGTCGCGATGAC‑TTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCTGCGTAGCATACATGAGGTTTTGTATAAAAATGGCGGGCGATATCAACGCAGTGTCAGAAATCCGAAACAGTCTCGCCTGGCGATAACCGTCTTGTCGGCGGTTGCGCTGACGTTGCGTCGTGATATCA > NC_000913/7695‑8183 | gtcacccGGGGGTAATTGCCGGCGCACGGCCGGCCAGGTGGCAGCTCCCCCCCCGCGCTGCAAGACGGGTACACAATGAGGTAAATGAGTGTAACCCGCCGGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGAT‑GTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGTGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGgag < 2:283527/246‑1 (MQ=255) cgctactggCCAGCGCAAGGCCTGCCAGGTGGCCTCGACCCCAGCGCGCCGAAAGACTGGTAAACAATTACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTGTAAGACTTGTGCCAAACTGGCGGAT‑GTAGCGAAACTGCACAAAACCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGGAAATCAGTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAGAAAATCt < 2:274353/243‑1 (MQ=255) tAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGAT‑GTAGCGAAACTGCACAAATCCGGTGCGAAAAGGGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGGGGAAAAATCTggg > 2:663552/1‑250 (MQ=255) aattgCCAGCGCACCGCCGGCCAGGTGGCCGCTACCCACGCGCTCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGCCTTTTGCCAAACTGGCGGAT‑GTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGgca < 1:376219/247‑1 (MQ=255) aatggccaggcaaaaaccGGCAAGTTTGCCCCTACCCACGCGGGCCTCAAGATTGTTACACAATTACGTAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTATGCCAAACTGGCGGAT‑GTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGgca < 2:67150/235‑1 (MQ=255) cAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGAT‑GTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCata < 1:508498/251‑1 (MQ=255) gcAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGAT‑GTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATccc < 1:663552/251‑1 (MQ=255) aGGTTGCCGATACCCAAGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGCCTTTTGCCAAACTGGCGGAT‑GGAGCGAAACTGCACAAATACGGTGCGAAAAGTGAACCAACAACCGGCGCAGCAGAGCAGGGAAATCATTACCGATACCCAAATGACGCTTGTAGTAAAGGAGAAAAAATCTGGCCAGCATATCCCTCTT‑ATTGCCgg > 2:519399/1‑251 (MQ=255) cTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATCAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGAT‑GTAGCGAAACTGCACAAATCCGGGGCGAAAAGTGAACCAACAACCTGGGCCGAAGAGCAGGGAAATCATTACCGATCCCCAAAGGACGCTGGTAATAAAGAAGAAAAAATCTTGCATGCTCATCCCTCTT‑ATTGCCggcaccgatg > 2:103408/1‑242 (MQ=255) cccACGCGCGCCGCAAGACTGTTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGAT‑GTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTT‑ATTGCCGGTCGCGATGAC‑tt < 1:657629/251‑1 (MQ=255) ccACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGAT‑GTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTT‑ATTGCCGGTCGCGATGAC‑ttt < 1:120823/251‑1 (MQ=255) cgcgcCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGAT‑GTAGCGAAACTGCACAAATCCGGTGCGAAAAGGGAACCAACAACCTGCGCCGGAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTT‑ATTGCCGGTCGCGATGAC‑TTTACtgt > 2:266422/1‑251 (MQ=255) ccgcAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGAT‑GTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTT‑ATTGCCGGTCGCGATGAC‑TTTCCTGTGTaa < 1:20626/251‑1 (MQ=255) cAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGATGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGGT‑GTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGCTCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTT‑ATTGCCGGTCGCGATGAC‑TTTCCTGTGTAAACg > 1:262154/1‑251 (MQ=255) aGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATTGTA‑CGAAACTGCACAAATCCGGTGCGAAAAGGGAACCAACAAACTGCGCCGAAGAACAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAAAAAAGGAGGAAAAATCTGGCATGCAAATCCCTCTT‑ATTTCCGGTCGCGAT‑ACATTTCCTGTGTAAAAGtt > 2:475894/1‑251 (MQ=255) gTACACAATGACGGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGAT‑GTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTT‑ATTGCCGGTCGCGATGAC‑TTTCCTGTGTAAACGTTACCAAt < 1:85672/251‑1 (MQ=255) gTACACAATGAATGAAAGGGGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGAT‑GTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTT‑ATTGCCGGTCGCGATGAC‑TTTCCTGTGTAAACGTTACCAAt < 2:522018/251‑1 (MQ=255) tACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGAT‑GTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGga > 2:220793/1‑181 (MQ=255) tACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGAT‑GTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGga < 1:220793/181‑1 (MQ=255) acaATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGAT‑GTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTT‑ATTGCCGGTCGCGATGAC‑TTTCCTGTGTAAACGTTACCAATTGtt < 1:199050/251‑1 (MQ=255) acaATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGAT‑GTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTT‑ATTGCCGGTCGCGATGAC‑TTTCCTGTGTAAACGTTACCAATTGtt < 1:516741/251‑1 (MQ=255) caagtaCTGAAAGTAGGTTAAACCGCATGGCTGTGTATGAATGCTATTTTTAAGAATTTTGCAAAACTGGCGGAT‑GTAGCGAAACTGCAAAAATCCGGTGCGAAAAGTGAACCAACAGCCTGCGCCGAAGAGCAGGTAAATCATGACAGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTT‑ATTGCCGGTCGCGATGAC‑TTTCCTGTGTAAACGTTACCAAtttttt < 2:364508/246‑1 (MQ=255) caATGACTGAAAGGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGAT‑GTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTT‑ATTGCCGGTCGCGATGAC‑TTTCCTGTGTAAACGTTACCAATTGttt < 1:195973/251‑1 (MQ=255) aTGACTGAAATGATGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGAT‑GTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTT‑ATTGCCGGTCGCGATGAC‑TTTCCTGTGTAAACGTTACCAATTGTTTaa < 1:103408/251‑1 (MQ=255) ggTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGAT‑GTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAACAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCGTATCCCTCTT‑ATTGCCGGTCGCGATGAC‑TTTCCTGTGTAAAAGTTACCAATTGTTTAAGAAGTATCTACGc > 1:646635/1‑251 (MQ=255) aaaCCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGAT‑GTAGCGAAACTGCACAAATCCGGAGCGCAAAGTGAACCAACAACATGCGCCAAAGAGCAGGTCAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTT‑ATTGCCAGTCGCGATGAC‑TTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACg > 1:413949/1‑251 (MQ=255) cGCCTGGCGGTGGATTAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGAT‑GTCGCGAAACTGTACAAATTCCGTGCGATAATTGACTCAACTACCTGCGCCGAAGCGCAGGTAAATCATTCCCGATCTCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTTATT‑CTGGTCTCGATGAC‑CTTCCGGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGt < 2:501607/251‑1 (MQ=255) gagggggATGAATGCTATTTTTAAGACGTTTGCCAATCTGGCGGAT‑GTAGCGAAACTGCTCAAATCCGTTGCGAAAAGTGAACCAACAACCTGCGTCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTGAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTT‑ATTGCCGGTCGCGACGAC‑TTTACTGTGTAAACGTGACCAATTGTTTAAGAAGTATATCCGCGACGATGTACTTGa < 2:227743/246‑1 (MQ=255) tgtgGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGAT‑GTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTT‑ATTGCCGGTCGCGATGAC‑TTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGAt > 1:337071/1‑250 (MQ=255) gtgGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGAT‑GTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTT‑ATTGCCGGTCGCGATGAC‑TTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATa < 1:526969/250‑1 (MQ=255) aaTGCTATTTTTAAGACTTTTGCCAAACTGGCGGAT‑GTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTT‑ATTGCCGGTCGCGATGAC‑TTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCTGc > 1:482912/1‑251 (MQ=255) cTTTTGCCAAACTGGCGGAT‑GTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTT‑ATTGCCGGTCGCGATGAC‑TTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCTGCGTAGCATACATGAGGt > 1:670600/1‑251 (MQ=255) ttttGCCAAACTGGCGGAT‑GTAGCGAAACTGCACAAATCCGGGGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTT‑ATTGCCGGTCGCGATGAC‑TTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGCACTTGATAACTTCTGCGTAGCATACATGAGGtt > 1:170649/1‑251 (MQ=255) gCCAAACTGGCGGAT‑GTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGCAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTT‑ATTGCCGGTCGCGATGAC‑TTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCTGCGTAGCATACCTGCGGATTTGt > 2:444413/1‑251 (MQ=255) cAAACTGGCGGAT‑GTAGCGAAACTGCCCAAATCCGGTGCGAAAAGTGAGCCAACAACCTGCGCCGAAGGGCAGGTAAATCATTACCGATCCCCAAAAGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTT‑ATTGCCGGTCGCGATGCT‑TTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGCGGTACTTGATAACTTCTACGAAGCATAcctgcgggtttggat > 1:209078/1‑237 (MQ=255) gAT‑GTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTT‑ATTGCCGGTCGCGATGAC‑TTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCTGCGTAGCATACATGAGGTTTTGTATAAAAATggcg > 1:464553/1‑251 (MQ=255) gTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAGCAGCCTGCGCCTAAGAGCAGGTAAAACATTACCGAGCCCCAAAGGACGCTGTTAAGAAAGTAGAAAAAATCGGGCATGCATATCCCTCTT‑ATTGCCGGTCGCGATGAC‑TTTCCTGTGTAAACGTTGCCAATTGGTTAAGAAGTATATACGCTACGAGTTACTTGATAACGTCTGCGTAGCATACATGAGGTTTTGTATATAAATggcgggc < 2:617306/251‑1 (MQ=255) aGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTT‑ATTGCCGGTCGCGATGAC‑TTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCTGCGTAGCATACATGAGGATTTGTATAAAAATggcgggcgg > 2:307438/1‑250 (MQ=255) aaaCTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTT‑ATTGCCGGTCGCGATGAC‑TTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCTGCGTAGCATACATGAGGTTTTGTATAAAAATGGCGGGCGATATc < 1:676572/251‑1 (MQ=255) cTGCACAAATACGGAGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCACAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTT‑ATTGCCGGTCGCGATGAC‑TTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCTGCGTAGCATACATGAGGTTTTGTATAAAAATGGCGGGGGatataaa > 2:428135/1‑247 (MQ=255) cacaAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGAACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTT‑ATTGCCGGTCGCGATGAC‑TTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCTGCGTAGCATCCATGAGGTTTTGTATACAAATGGCGGGCGGTATccacac > 2:263319/1‑245 (MQ=255) aaaTCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTT‑ATTGCCGGTCGCGATGAC‑TTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCTGCGTAGCATACATGAGGTTTTGTATAAAAATGGCGGGCGATATCAACGCAgtg < 1:415830/251‑1 (MQ=255) cGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTT‑ATTGCCGGTCGCGATGAC‑TTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCTGCGTAGCATACATGAGGTTTTGTATAAAAATGGCGGGCGATATCAACGCAGTGTCAGa < 2:464553/251‑1 (MQ=255) cGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTT‑ATTGCCGGTCGCGATGAC‑TTCCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCTGCGTAACATACATGAGGTTTTGTATAAAAATGGCGGGGGATGTAAACGCAgtgaca > 2:173988/1‑246 (MQ=255) gTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGCGAACACATCTGGCATGCATATCCCTCTT‑ATTGCCGGTCGCGATGAC‑TTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGAACTTGATAACTTCTGCGTAGCATACATGAGGTTTTGTATAAAAATGGCGGGCGATATCCACGCCGTGTCAgga > 1:273343/1‑248 (MQ=255) cGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTT‑ATTGCCGGTCGCGATGAC‑TTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGt < 1:488088/158‑1 (MQ=255) cGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTT‑ATTGCCGGTCGCGATGAC‑TTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGt > 2:488088/1‑158 (MQ=255) aaGTGAACCTACAACCTGCGCCGAAGAGCAGGTAAATCATTAAAGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTT‑ATTGCCGGTCGCGATGAC‑TTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCTGCGTAGAATACATGAGGTTTTGTATAAAAATGGCGGGGGATATCAACGCaaatgcacaaattcgaa > 2:444666/1‑234 (MQ=255) aaGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTT‑ATTGCCGGTCGCGATGAC‑TTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCTGCGTAGCATACATGAGGTTTTGTATAAAAATGGCGGGCGATATCAACGCAGTGTCAGAAATCCGaaa < 1:468907/251‑1 (MQ=255) ccAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTT‑ATTGCCGGTCGCGATGAC‑TTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCTGCGTAGCATACATGAGGTTTTGTATAAAAATGGCGGGCGATATCAACGCAGTGTCAGAAATCCGAAACAGtctc < 1:493752/251‑1 (MQ=255) agagCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTT‑ATTGCCGGTCGCGATGAC‑TTTCCTGTGTAAACGtt < 1:517324/112‑1 (MQ=255) agagCAAGAAAATCCTTACCGATCCCCACAGGACTCTGTTAATAAAGGAGGAAAAATAGGGCATGCATATCCATCTT‑ATTGCCGGTCGCGATGAC‑TTTCCTGTGTAAACGtt > 2:517324/1‑112 (MQ=255) aGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTT‑ATTGCCGGTCGCGATGAC‑TTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCTGCGTAGCATACATGAGGTTTTGTATAAAAATGGCGGGCGATATCAACGCAGTGTCAGAAATCCGAAACAGTCTCGCCTGGCGATAACCGTCTTGTc < 1:225550/251‑1 (MQ=255) tCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTT‑ATTGCCGGTCGCGATGAC‑TTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCTGCGTAGCATACATGAGGTTTTGTATAAAAATGGCGGGCGATATCAACGCAGTGTCAGAAATCCGaa < 2:128125/214‑1 (MQ=255) tCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTT‑ATTGCCGGTCGCGATGAC‑TTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCTGCGTAGCATACATGAGGTTTTGTATAAAAATGGCGGGCGATATCAACGCAGTGTCAGAAATCCGaa > 1:128125/1‑214 (MQ=255) tACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTT‑ATCGCCGGTCGCGATGAC‑TTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCTGCATAGCATACATGAGGTTTTGTATAAAAATGGCGGGCGATATCAACGCAGTGTTAGAAATCCGAAACAGTCTCGCCTGGCGATAACCGTCTTGTcggcgggtgtgc > 1:322110/1‑245 (MQ=255) tACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTT‑ATTGCCGGTCGCGATGAC‑TTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCTGCGTAGCATACATGAGGTTTTGTATAAAAATGGCGGGCGATATCAACGCAGTGTCAGAAATCCGAAACAGTCTCGCCTGGCGATAACCGTCTTGTCGGCGGGTgcgc > 1:569366/1‑251 (MQ=255) aGGACGCTGTTAATAAAGGAGAAAAATTCTGGCATGCATATCCCTCTT‑ATTGCCGGTCGCGATGAC‑TTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCTGCGTAGCATACATGAGGTTTTGTATAAAAATGGCGGGCGATATCAACGCAGTGTCAGAAATCCGAAACAGTCTCGCCTGGCGATAACCGTCTTGTCGGCGGTTGCGCTGACGTTGcgtcg < 1:49004/251‑1 (MQ=255) gTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTT‑ATTGCCGGTCGCGATGAC‑TTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCTGCGTAGCATACATGAGGTTTTGTATAAAAATGGCGGGCGATATCAACGCAGTGTCAGAAATCGGAAACAGTCTCGCCTGGCGATAACCGTCTTGTCGGCGGTTGCGCTGACGTTGCGTCGTGATatca < 1:330703/251‑1 (MQ=255) | GTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGAT‑GTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTT‑ATTGCCGGTCGCGATGAC‑TTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCTGCGTAGCATACATGAGGTTTTGTATAAAAATGGCGGGCGATATCAACGCAGTGTCAGAAATCCGAAACAGTCTCGCCTGGCGATAACCGTCTTGTCGGCGGTTGCGCTGACGTTGCGTCGTGATATCA > NC_000913/7695‑8183 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |