Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,641,544	A→G	Q201Q (CAA→CAG)	yjtD →	putative tRNA/rRNA methyltransferase YjtD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,641,544	0	A	G	100.0%	171.5 / NA	53	Q201Q (CAA→CAG)	yjtD	putative tRNA/rRNA methyltransferase YjtD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/26);  total (27/26)

CTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCGGATTATCCTTCGCTCAATCTGGGGCAGGCGGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGGCGAAAAGTGATGCAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGCCATGACATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAACAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4641300‑4641652                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                  cTAACGAAGAGTTAGAGTTGGCTGACGTTCTTACTGGTGTGCCGATGGAGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGTCCGTGGCAGATGAAATAAAACTGGTCGACTGGTTACAGCAACGc                                                                                                                                                                                                                                             >  2:663495/1‑251 (MQ=255)              aGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑ag                                                                                                                                                                                                                               <  1:447802/251‑1 (MQ=255)                        gACGTTCTTACTGGTGGGCCGATGGTGGCAGATTATCCTTCGCGCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACgaga                                                                                                                                                                                                                     <  1:632161/251‑1 (MQ=255)                         aCGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGac                                                                                                                                                                                                                    >  1:168758/1‑251 (MQ=255)                               ttACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGAATGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGc                                                                                                                                                                                                               >  1:505968/1‑250 (MQ=255)                                 aCTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAAt                                                                                                                                                                                                            <  1:426633/251‑1 (MQ=255)                                     gtgtGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACg                                                                                                                                                                                                                 <  1:290186/242‑1 (MQ=255)                                     gtgtGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAAAGAGAAACgagatcgg                                                                                                                                                                                                          >  2:290186/1‑242 (MQ=255)                                      tgtgCCGCTGGTGGAAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGATGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCCACGCCTGGGGCTTTTCAGGACAA‑GAGAAACGGCAATGtttc                                                                                                                                                                                                       >  2:609754/1‑249 (MQ=255)                                           cGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGGTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGAAATGTTGCACCgg                                                                                                                                                                                                  >  1:459086/1‑250 (MQ=255)                                               ggtggCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTtgc                                                                                                                                                                                              >  1:84677/1‑251 (MQ=255)                                                   gcagATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCa                                                                                                                                                                                          <  1:45680/248‑1 (MQ=255)                                                            ccTTCGCTCAATCGTGGGCAGGCAGTGCTGGTCTTTTGCTACCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCATCTGCAAGCTTTACGCGAACTAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGGCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGa                                                                                                                                                                                 <  2:462160/251‑1 (MQ=255)                                                                  cTCAATCTGGGGCAGGCAGTGATGGTCTATTGATATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCACGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGACTGGGGCTTTT‑AGAGCACCGAGACACGGCAATGTTGCACCGTTTGCTGCATGCAATTGaaaaca                                                                                                                                                                            >  1:317139/1‑248 (MQ=255)                                                                    cAATCTGGGGTAGTCAGTGATGGTTTATTTCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAAACCAACATCAACTGCAAGCTGTCCGCGAACGAGTCATGTCATTTCTGACGTCTCTGTCAGTGGCTGATGCCATAAAACTGGGCAACTGGTTACAGCAACGCCTGGCGCTTTT‑ATAGCAACGAGAAACGGCAATGTTGCGCAGTTTGCTGCATGATATTGaaaaaatta                                                                                                                                                                         <  2:505968/251‑4 (MQ=255)                                                                          ggggCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCa                                                                                                                                                                      <  1:313924/248‑1 (MQ=255)                                                                               aGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATaa                                                                                                                                                              <  1:672066/251‑1 (MQ=255)                                                                                 gcagTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATaaaa                                                                                                                                                            <  1:419488/248‑1 (MQ=255)                                                                                 gcagTGATGGGCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCGTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATaaaa                                                                                                                                                            <  1:648342/248‑1 (MQ=255)                                                                                       aTGGGCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACGGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATAGCACCAAATAAAAAACGcc                                                                                                                                                      <  2:136783/251‑1 (MQ=255)                                                                                                 gCTATCAATTAGCAACATTACTACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCCCCGTTtgct                                                                                                                                                                                             >  2:201425/1‑202 (MQ=255)                                                                                                 gCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTtgct                                                                                                                                                                                             <  1:201425/202‑1 (MQ=255)                                                                                                          tAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTc                                                                                                                                      >  1:299430/1‑248 (MQ=255)                                                                                                          aagaaacactAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTAACGCGAACGAGTCATGGCAGTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTAGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTCag                                                                                                                                    >  2:299430‑M2/5‑248 (MQ=255)                                                                                                            gCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTCagctt                                                                                                                                 >  1:248383‑M2/1‑246 (MQ=255)                                                                                                             cAACATTAATAAAACAACCGACGAAAAGGGACACAACGGCCGCCCAACATCAACTGCAAGCTTTACGAGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGCCTGGTTACAGCCACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGGTGCACCGTTTGCTGCATGATTTTGAGAAAAATCTCACCAAATAAAAAACGACTTAGTACGTATTTTTcagcttt                                                                                                                                >  2:100764/1‑245 (MQ=255)                                                                                                              aaCATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACAAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGCCTCTGGCAGTGGCAGCTGACATCAAACTGGTCAACTGGTTACAGCAAAGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGGTTGCTGCATGATATTGAAAAAAATATAACCAAATAAAAAACGCCTTATAAAGTATTTTTTagctttt                                                                                                                               <  2:585642‑M2/251‑8 (MQ=255)                                                                                                                cATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTCagcttttc                                                                                                                              <  1:310760‑M2/250‑9 (MQ=255)                                                                                                                 aTTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTCagcttttcat                                                                                                                            <  1:323976‑M2/251‑11 (MQ=255)                                                                                                                  ataAAACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTCagcttttcatt                                                                                                                           >  2:323976‑M2/2‑240 (MQ=255)                                                                                                                       acaacaACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAA‑CGCCTTAGTAAGTATTTTTCagcttttcattctgac                                                                                                                      >  1:351304‑M2/1‑234 (MQ=255)                                                                                                                                gacgaAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTCagcttttcattctgactgcaacggg                                                                                                             >  1:356518‑M2/3‑226 (MQ=255)                                                                                                                                     aaaGTGACACAACGGCAGACCCACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATACAAAACGCCTTAGTAAGTATTTTTCagcttttcattctgactgcaacgggcaat                                                                                                         <  2:304990‑M2/250‑30 (MQ=255)                                                                                                                                        gtgacacaacggcAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTCagcttttcattctgactgcaacgggcaatatgt                                                                                                     >  1:581653‑M2/7‑218 (MQ=255)                                                                                                                                             acaACGGCAGACCAACAGCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCTACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTCagctttgcattctgactgccacgggcaatatgtctctg                                                                                                >  2:36120‑M2/2‑213 (MQ=255)                                                                                                                                             acAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAata                                                                                                                                                                         >  1:517719/2‑178 (MQ=255)                                                                                                                                                               cAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTCagcttttcattctgactgcaacgggcaatatgtctctgtgtggattaaaaaaagag                                                                              >  1:100764‑M2/1‑195 (MQ=255)                                                                                                                                                                aaCTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTCagcttttcattctgactgcaacgggcaatatgtctctgtgtggattaaaaaaagagt                                                                             <  1:68481‑M2/251‑58 (MQ=255)                                                                                                                                                                                              cATTGCTGACGGCTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTCagcttttcattctgactgcaacgggcaatatgtctctgtgtggattaaaaaaagagtgtctgatagcagcttctgaactggttacct                                               >  2:84677‑M2/1‑164 (MQ=255)                                                                                                                                                                                                     gacgacTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTCagcttttcattctgactgcaacgggcaatatgtctctgtgtggattaaaaaaagagtgtctgatagcagcttctgaactggttacctgccgtga                                        >  1:585642‑M2/1‑157 (MQ=255)                                                                                                                                                                                                     gacgacTCTGGCAGTGGCAGATAACATAAAACGGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGAATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTCagcttttcattctgactgcaacgggcaatatgtctttgtgtggattaaaaaaagagtgtctgatagcagcttctgaactggttacctgccgtga                                        >  2:644999‑M2/1‑157 (MQ=255)                                                                                                                                                                                                         acTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTCagcttttcattctgactgcaacgggcaatatgtctctgtgtggattaaaaaaagagtgtctgatagcagcttctgaactggttacctgccgtgagta                                     <  1:570276‑M2/250‑98 (MQ=255)                                                                                                                                                                                                              ggcagtggcagATGACATAAAACTGGTCGCCTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTCagcttttcattctgactgcaacgggcaatatgtctctgtgtggattaaaaaaagagtgtctgatagcagcttctgaactggttacctgccgtgagtaaatta                                <  1:525835‑M2/250‑103 (MQ=255)                                                                                                                                                                                                               gcagtggcagATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTCagcttttcattctgactgcaacgggcaatatgtctctgtgtggattaaaaaaagagtgtctgatagaagcttctgaactggttacctgccgtgagtaaattac                               <  2:641063‑M2/250‑104 (MQ=255)                                                                                                                                                                                                               gcagtggcagATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAAAGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTCagcttttcattctgactgcaacgggcaatatgtctctgtgtggattataaaaagagtgtctgatagcagcttctgaactggttacctgccgtgagtaaattca                               <  2:130185‑M2/250‑104 (MQ=255)                                                                                                                                                                                                                      cagATTACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCCCCAAATAAAAAACGCCTTAGTAAGTATTTTTCagcttttcattctgcctgcaacgggcaatatgtctctgtttggattaaaaaaagagtgtctgatagcagcttctgaactggttacctgccgtgagtaaattaaaattttat                       >  2:323082‑M2/1‑140 (MQ=255)                                                                                                                                                                                                                          tGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGTGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTCagcttttcattctgactgcaacgggcaatatgtctc                                                                                                  >  2:200001‑M2/1‑136 (MQ=255)                                                                                                                                                                                                                          tGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTCagcttttcattctgactgcaacgggcaatatgtctc                                                                                                  <  1:200001‑M2/172‑37 (MQ=255)                                                                                                                                                                                                                                  aaCTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGGTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTCagcttttcattctgactgcaacgggcaatatgtctctgtgtggattaaaaaaagagtgtctgatagcagcttctgaactggttacctgccgtgagtaaattaaaattttattgacttaggtc            <  2:430861‑M2/250‑123 (MQ=255)                                                                                                                                                                                                                                   aCTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTCagcttttcattctgactgcaacgggcaatatgtctctgtgtggattaaaaaaagagtgtctgatagcagcttctgaactggttacctgccgtgagtaaattaaaattttattgacttaggtcac          >  1:663495‑M2/1‑127 (MQ=255)                                                                                                                                                                                                                                      ggTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTCagcttttc                                                                                                                              >  2:311522‑M2/1‑124 (MQ=255)                                                                                                                                                                                                                                      ggTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTCagcttttc                                                                                                                              <  1:311522‑M2/132‑9 (MQ=255)                                                                                                                                                                                                                                           aCTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTAGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAACACGCCTTAGTAAGAATTTTTCagctttacattctgactgcaacgggcaaaatgtctctgtgtagattaaaaaaatagtgtctgatagcagcttctgaactggttacctgccgagggtaatttaaaattttcttgacttaggacactaactact  <  2:503385‑M2/251‑133 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                  CTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCGGATTATCCTTCGCTCAATCTGGGGCAGGCGGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGGCGAAAAGTGATGCAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGCCATGACATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAACAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4641300‑4641652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑