Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,641,483	C→T	A181V (GCC→GTC)	yjtD →	putative tRNA/rRNA methyltransferase YjtD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,641,483	0	C	T	100.0%	182.2 / NA	59	A181V (GCC→GTC)	yjtD	putative tRNA/rRNA methyltransferase YjtD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/28);  total (31/28)

CTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGTTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCGGATTATCCTTCGCTCAATCTGGGGCAGGCGGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGGCGAAAAGTGATGCAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGCCATGACATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAACAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4641246‑4641652                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                    cTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGCTTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGCGTCATGGCATTGCTg                                                                                                                                                                                                                        >  1:312749/1‑251 (MQ=255) cTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTg                                                                                                                                                                                                                        >  1:237463/1‑251 (MQ=255)   tcggggATGAGCCATGCTGCGCGGGTGTGTGGTCGCGAAGATCACGGGCTGACTAACGAAGAGTTAGCGGTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCTCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACAGCAACTGCAAGCTTGACGCGAACGAGTCATGGCATTGcg                                                                                                                                                                                                                         <  2:309752/244‑2 (MQ=255)    cATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAAAGGCAGACAAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTgacg                                                                                                                                                                                                                     >  1:562866/1‑251 (MQ=255)    cATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACGAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACGGCAAGCTTTACGCGAACGAGTCATGGCATTGCTgac                                                                                                                                                                                                                      <  2:76388/250‑1 (MQ=255)     aTGGCTGAGCCATGCCGAGCTGGTGTTTGGTCGCGAAGATTCCGTGCTGACTAACGAAGCGTTAGCGTTGGCTGACGGTCTTACTGGTGTGCCGATGGAGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGCTGGTCTATTGCTATCAATTAGCAACATTAATACAAAAACCGCCGAAAAGTGACACAACGGCAGACCACAATCAACAGCAAGCTATACGCGAACGCGTCATGGCATAGCTgacac                                                                                                                                                                                                                    >  2:447802/1‑249 (MQ=255)     aTGGATGAGCCATGCCGCGCTGGTGTTTGGTAGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGACGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCCGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACAAACATCAACCGCAAGCTTTACGCGAACGAGTCATGGCATTGCTaacgc                                                                                                                                                                                                                    >  2:419488/1‑250 (MQ=255)      tggtgggggcaggCCGCGCTGGTGTTTTGTCGCGAAGATGCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGTCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATGAATACAACAACCTACGAAAAGTGACACAACGGCAGACCAACATCAACCGCAAGCTTTACGCGAACGAGTCATTGCATTGCTgac                                                                                                                                                                                                                      <  2:253360/236‑1 (MQ=255)            aGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTAACACAACGGCAGACCAACATACACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCtggc                                                                                                                                                                                                             >  2:248383/1‑251 (MQ=255)             gCCATGCCGAGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGCTGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAACGTGACACAAAGGCAGACCAAAATCAACTGAAAGCTTTACGCGACCGAGTCATGGCATTGCTGACGACTCtggc                                                                                                                                                                                                             >  2:659286/1‑250 (MQ=255)                       gcTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGGGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATg                                                                                                                                                                                                  >  1:356824/1‑251 (MQ=255)                          ggggtTTGGTCGCGAATATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGGTCTTACTGGGGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACa                                                                                                                                                                                               <  2:166650/248‑1 (MQ=255)                           gtgtTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACAt                                                                                                                                                                                              <  1:659286/251‑1 (MQ=255)                             gtTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATa                                                                                                                                                                                             >  1:416180/1‑250 (MQ=255)                                tGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATaaaa                                                                                                                                                                                          <  1:25293/250‑1 (MQ=255)                                      cgAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCg                                                                                                                                                                                   >  1:36120/1‑251 (MQ=255)                                          gATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGCTTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAACGTGACCCAACGGCAGACCAACATCCACTGCCAGCTTTACGCGAACGAGTCATGCCTTTGCTGACGACTCTGGCAGTGGCAGCTGACATaaatctagccgac                                                                                                                                                                                 >  2:426633/1‑239 (MQ=255)                                             tCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGt                                                                                                                                                                             <  2:416180/250‑1 (MQ=255)                                               cGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTac                                                                                                                                                                          <  1:64595/251‑1 (MQ=255)                                                 gtctgattaactaatagTTAGCGGTGGCTGACGTTCTGCCTGGTGTGCCGATGGTGGCAGATTTTCCTTCGCTCAAGCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACTAAAAGTGACACAACGGCAGACCAACAGCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTTGttccag                                                                                                                                                                        <  2:562866/236‑5 (MQ=255)                                                 ggctGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTacag                                                                                                                                                                        <  2:183533/248‑2 (MQ=255)                                                       cTAACGAAGAGTTAGAGTTGGCTGACGTTCTTACTGGTGTGCCGATGGAGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGTCCGTGGCAGATGAAATAAAACTGGTCGACTGGTTACAGCAACGc                                                                                                                                                                  >  2:663495/1‑251 (MQ=255)                                                                    aGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑ag                                                                                                                                                    <  1:447802/251‑1 (MQ=255)                                                                              gACGTTCTTACTGGTGGGCCGATGGTGGCAGATTATCCTTCGCGCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACgaga                                                                                                                                          <  1:632161/251‑1 (MQ=255)                                                                               aCGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGac                                                                                                                                         >  1:168758/1‑251 (MQ=255)                                                                                     ttACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGAATGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGc                                                                                                                                    >  1:505968/1‑250 (MQ=255)                                                                                       aCTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAAt                                                                                                                                 <  1:426633/251‑1 (MQ=255)                                                                                           gtgtGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACg                                                                                                                                      <  1:290186/242‑1 (MQ=255)                                                                                           gtgtGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAAAGAGAAACgagatcgg                                                                                                                               >  2:290186/1‑242 (MQ=255)                                                                                            tgtgCCGCTGGTGGAAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGATGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCCACGCCTGGGGCTTTTCAGGACAA‑GAGAAACGGCAATGtttc                                                                                                                            >  2:609754/1‑249 (MQ=255)                                                                                                 cGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGGTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGAAATGTTGCACCgg                                                                                                                       >  1:459086/1‑250 (MQ=255)                                                                                                     ggtggCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTtgc                                                                                                                   >  1:84677/1‑251 (MQ=255)                                                                                                         gcagATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCa                                                                                                               <  1:45680/248‑1 (MQ=255)                                                                                                                  ccTTCGCTCAATCGTGGGCAGGCAGTGCTGGTCTTTTGCTACCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCATCTGCAAGCTTTACGCGAACTAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGGCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGa                                                                                                      <  2:462160/251‑1 (MQ=255)                                                                                                                        cTCAATCTGGGGCAGGCAGTGATGGTCTATTGATATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCACGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGACTGGGGCTTTT‑AGAGCACCGAGACACGGCAATGTTGCACCGTTTGCTGCATGCAATTGaaaaca                                                                                                 >  1:317139/1‑248 (MQ=255)                                                                                                                          cAATCTGGGGTAGTCAGTGATGGTTTATTTCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAAACCAACATCAACTGCAAGCTGTCCGCGAACGAGTCATGTCATTTCTGACGTCTCTGTCAGTGGCTGATGCCATAAAACTGGGCAACTGGTTACAGCAACGCCTGGCGCTTTT‑ATAGCAACGAGAAACGGCAATGTTGCGCAGTTTGCTGCATGATATTGaaaaaatta                                                                                              <  2:505968/251‑4 (MQ=255)                                                                                                                                ggggCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCa                                                                                           <  1:313924/248‑1 (MQ=255)                                                                                                                                     aGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATaa                                                                                   <  1:672066/251‑1 (MQ=255)                                                                                                                                       gcagTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATaaaa                                                                                 <  1:419488/248‑1 (MQ=255)                                                                                                                                       gcagTGATGGGCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCGTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATaaaa                                                                                 <  1:648342/248‑1 (MQ=255)                                                                                                                                             aTGGGCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACGGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATAGCACCAAATAAAAAACGcc                                                                           <  2:136783/251‑1 (MQ=255)                                                                                                                                                       gCTATCAATTAGCAACATTACTACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCCCCGTTtgct                                                                                                                  >  2:201425/1‑202 (MQ=255)                                                                                                                                                       gCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTtgct                                                                                                                  <  1:201425/202‑1 (MQ=255)                                                                                                                                                                tAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTc                                                           >  1:299430/1‑248 (MQ=255)                                                                                                                                                                aagaaacactAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTAACGCGAACGAGTCATGGCAGTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTAGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTCag                                                         >  2:299430‑M2/5‑248 (MQ=255)                                                                                                                                                                  gCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTCagctt                                                      >  1:248383‑M2/1‑246 (MQ=255)                                                                                                                                                                   cAACATTAATAAAACAACCGACGAAAAGGGACACAACGGCCGCCCAACATCAACTGCAAGCTTTACGAGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGCCTGGTTACAGCCACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGGTGCACCGTTTGCTGCATGATTTTGAGAAAAATCTCACCAAATAAAAAACGACTTAGTACGTATTTTTcagcttt                                                     >  2:100764/1‑245 (MQ=255)                                                                                                                                                                    aaCATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACAAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGCCTCTGGCAGTGGCAGCTGACATCAAACTGGTCAACTGGTTACAGCAAAGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGGTTGCTGCATGATATTGAAAAAAATATAACCAAATAAAAAACGCCTTATAAAGTATTTTTTagctttt                                                    <  2:585642‑M2/251‑8 (MQ=255)                                                                                                                                                                      cATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTCagcttttc                                                   <  1:310760‑M2/250‑9 (MQ=255)                                                                                                                                                                       aTTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTCagcttttcat                                                 <  1:323976‑M2/251‑11 (MQ=255)                                                                                                                                                                        ataAAACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTCagcttttcatt                                                >  2:323976‑M2/2‑240 (MQ=255)                                                                                                                                                                             acaacaACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAA‑CGCCTTAGTAAGTATTTTTCagcttttcattctgac                                           >  1:351304‑M2/1‑234 (MQ=255)                                                                                                                                                                                      gacgaAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTCagcttttcattctgactgcaacggg                                  >  1:356518‑M2/3‑226 (MQ=255)                                                                                                                                                                                           aaaGTGACACAACGGCAGACCCACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATACAAAACGCCTTAGTAAGTATTTTTCagcttttcattctgactgcaacgggcaat                              <  2:304990‑M2/250‑30 (MQ=255)                                                                                                                                                                                              gtgacacaacggcAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTCagcttttcattctgactgcaacgggcaatatgt                          >  1:581653‑M2/7‑218 (MQ=255)                                                                                                                                                                                                   acaACGGCAGACCAACAGCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCTACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTCagctttgcattctgactgccacgggcaatatgtctctg                     >  2:36120‑M2/2‑213 (MQ=255)                                                                                                                                                                                                   acAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAata                                                                                              >  1:517719/2‑178 (MQ=255)                                                                                                                                                                                                                     cAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTCagcttttcattctgactgcaacgggcaatatgtctctgtgtggattaaaaaaagag   >  1:100764‑M2/1‑195 (MQ=255)                                                                                                                                                                                                                      aaCTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTCagcttttcattctgactgcaacgggcaatatgtctctgtgtggattaaaaaaagagt  <  1:68481‑M2/251‑58 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                    CTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGTTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCGGATTATCCTTCGCTCAATCTGGGGCAGGCGGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGGCGAAAAGTGATGCAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGCCATGACATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAACAACGCCTGGGGCTTTT‑AGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4641246‑4641652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑