Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 2,718,617 IS2 (–) +5 bp intergenic (‑89/‑114) yfiF ← / → trxC putative methyltransferase YfiF/reduced thioredoxin 2

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 2718617 =2 (0.030)48 (0.800) 45/482 0.3 95.9% intergenic (‑89/‑118) yfiF/trxC putative methyltransferase YfiF/reduced thioredoxin 2
?NC_000913 = 2997691 NA (NA)noncoding (1/1331 nt) IS2 repeat region
* ? NC_000913 = 27186212 (0.030)61 (1.010) 55/482 0.2 96.8% intergenic (‑93/‑114) yfiF/trxC putative methyltransferase YfiF/reduced thioredoxin 2
?NC_000913 2996361 = NA (NA)noncoding (1331/1331 nt) IS2 repeat region

TTGCTTACGCCAGAGAAATAACTGGCTGGCTGCTACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTTCTCTGTAATGTTTATCTTTTTCCAGATAACCTGGGGATTCTCCCCCTAAATCAAGCTGTAACGTTATTCTTTCATAACCTTTATCTATGAGGTGGTTCAACCTCGTTGTTGAAAGAGTTTCATCCAGTTCATTGACTTTAGCTTCGCGTAGTAAATCAGAGACA  >  NC_000913/2997445‑2997932
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 
ttGCTTACGCCAGAGAAATAACTGGCTGGCTGCTCCACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACt                                                                                                                                                                                                                                               >  1:187359/1‑251 (MQ=11)
  gCTTACGACAGATAAATAACTGGCTGGCTGCTACACCATGGTGCCGGGCAACGAGTGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTGCCTGTGTGGTACGCCGTCTGCGTTTCTCCTGCCCTAAGACATCAATCATCTGTGCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCTCTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCTAGTCTAAACtt                                                                                                                                                                                                                                              <  2:570669/250‑1 (MQ=11)
      aCGCCAGAGAAATAACTGGCTGGCTGCTTCACCATGTTGCCGGGCAACGAGGTAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTTTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTTATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACTTGctct                                                                                                                                                                                                                                         <  1:242561/251‑1 (MQ=12)
            gataatgaaCTGGCTCGCTTCTACACCTTGGTGCCGTGCAACTCGTGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGACCACATGCGCTGTTTTCCTTTGTTGTACGCCGTCTGCGATTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACGATTCTTAAAACTAGTATTAAGGCTATTACTTATTTAATTGATATTGGGTGTCTTGAAATTCATGGGGCCAGTCTAAACTTGCTCtttccgt                                                                                                                                                                                                                                   <  2:231347/244‑5 (MQ=2)
                aaTAACTGGCTGGCTGCTACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACTTGCTCTTTTCTTCTgg                                                                                                                                                                                                                               >  1:157631/1‑251 (MQ=37)
                                    accctGTTGCCTGGCAACGAGTGAGTACGTCATCCCCATTTTATAGCTCTGCTGAACAATGGTGATCGTTTCCTGTGTGGTACGTCGTCTGCTTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAACAATAGTATTAAGACTATCACTGATTGAAGGGATATTGTTTGTCGTGATATTCAGGGGGCCAGTTTAAACTTGCTCTTGTCTTCTGATGTTTATCTTTGATAGAttt                                                                                                                                                                                                           <  2:198402/247‑1 (MQ=9)
                                                                        cGGTTCAAAGCGCTGCTTAACAATTGCGATCTTTTCATGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACAACAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCt                                                                                                                                                                       <  2:22155/251‑1 (MQ=255)
                                                                           ttCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCACTGACTAGTCTAAAACCTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGTTTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTtct                                                                                                                                                                    >  2:383346/1‑251 (MQ=255)
                                                                               aaGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTTCTCTGt                                                                                                                                                                <  2:241851/251‑1 (MQ=255)
                                                                                              aTTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTTCTCTGTAATGTTTATCttttt                                                                                                                                                 >  1:220901/1‑251 (MQ=255)
                                                                                                   gATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTTCTCTGTAATGTTTATCTTTTTcc                                                                                                                                               >  1:512495/1‑248 (MQ=255)
                                                                                                     tCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTTCTCTGTAATGTTTATCTTTTTCCAGAt                                                                                                                                           >  1:565045/1‑250 (MQ=255)
                                                                                                       tgttCCTGTGTGGTACGCAGCCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGGGATTCAAGGGGCCAGTCTAATCTTGATCTTTTCTTCTGGTGTTTATCTTTGCAAGCTTTGTAGGAAGCATAACATTACGAAAAGCCGCATCGTCTTCTCTGTCATGTTTATCTTTTTCCAGATaa                                                                                                                                         >  2:485714/3‑250 (MQ=18)
                                                                                                                 cggTACGCCGTCTGCCTTTCCCCGGCCCTAAGACATCAATCAGCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTTCTCTGTAATGTTTATCTTTTTCCAGATAACCTGGGGATtc                                                                                                                              <  2:233570/250‑1 (MQ=255)
                                                                                                                    tACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTTCTCTGTAATGTTTATCTTTTTCCAGATAACCTGGGGATTCTcc                                                                                                                           >  1:573446/1‑251 (MQ=255)
                                                                                                                                 tttCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGTTAAATAACATTAAGTAAAGCCGCATCGGCTTCTCTGTAATGTTTATCTTTTTCCAGATAACCTGGGGATTCTCCCCCTAAATCAAGc                                                                                                              >  1:24775/1‑251 (MQ=255)
                                                                                                                                                gACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTTCTCTGTAATGTTTATCTTTTTCCAGATAACCTGGGGATTCTCCCCCTAAATCAAGCTGTAACGTTATTCtt                                                                                               <  1:430626/251‑1 (MQ=255)
                                                                                                                                                                                 aaaaaCTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTTCTCTGTAATGTTTATCTTTTTCCAGATAAACTGGGGATTCTCCCCCTAAATCAAGCTGTAACGTTATTCTTTAATAACCTTTATCTATGAGGTGGTTCAACCTc                                                              >  2:151907/1‑251 (MQ=255)
                                                                                                                                                                                     aCTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTTCTCTGTAATGTTTATCTTTTTCCAGATAACCTGGGGATTCTCCCCCTAAATCaa                                                                                                                <  1:350954/197‑1 (MQ=255)
                                                                                                                                                                                     aCTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTTCTCTGTAATGTTTATCTTTTTCCAGATAACCTGGGGATTCTCCCCCTAAATCaa                                                                                                                >  2:350954/1‑197 (MQ=255)
                                                                                                                                                                                              aaGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGTCCAGTCTAAACTTGCTCTTTTCTTCTGGTGTTTAGCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTTCTCTGTAATGTTTATCTTTTTCCAGATAACCTGGGGATTCTCCCCCTAAATCAAGCTGTAACGTTATTCTTTCATAACCTTTATCTATGAGGTGGTTCAACCTCGTTGTTGAAaga                                                  <  1:658091/250‑1 (MQ=255)
                                                                                                                                                                                                gACTATCACTTATTTCAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTTCTCTGTAATGTTTATCTTTTTCCAGATAACCTGGGGATTCTCCCCCTAAATCAAGCTGTAACGTTATTCTTTCATAACCTTTATCTATGAGGTGGTTCAACCTCGTTGTTGAAAGCGtt                                               >  2:581413/1‑251 (MQ=255)
                                                                                                                                                                                                gACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTTCTCTGTAATGTTTATCTTTTTCCAGATAACCTGGGGATTCTCCCCCTAAATCAAGCTGTAACGTTATTCTTTCATAACCTTTATCTATGAGGTGGTTCAACCTCGTTGTTGAAAGAGtt                                               >  1:308144/1‑251 (MQ=255)
                                                                                                                                                                                                 aCTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTTCTCTGTAATGTTTATCTTTTTCCAGATAACCTGGGGATTCTCCCCCTAAATCAAGCTGTATCGTTATTCTTTCATAACCTTTATCTATGAGGTGGTTCACCCTCGTTGTTGAAAGAGttt                                              >  2:107416/1‑251 (MQ=255)
                                                                                                                                                                                                    aTCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTTCTCTGTAATGTTTATCTTTTTCCAGATAACCTGGGGATTCTCCCCCTAAATCAAGCTGTAACGTTATTCTTTCATAACCTTTATCTATGAGGTGGTTCAACCTCGTTGTTGAAAGAGTTTCa                                            <  1:109058/250‑1 (MQ=255)
                                                                                                                                                                                                                agggtttatggttggtgtgagaTTCAGGGGGTCAGTCTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGTTAAATAACATTAAGTAAAGCCGCATCGGCTTCTCTGGAATGTTTATCTTTTTCCAGATAACCTGGGTATTCTCCCCCTGAATCAAGCTGTAACGTTATTCTTTCATAACCTTTATCTATTAGGTGGTTTAACCTCGTTGTTGAAAGAGTTTCATCCAGTTCAtgga                               <  2:565045/233‑4 (MQ=255)
                                                                                                                                                                                                                                tGGAGATTCAGGGGGCCAGTCTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTTCTCTGTAATGTTTATCTTTTTCCAGATAACCTGGGGATTCTCCCCCTAAATCAAGCTGTAACGTTATTCTTTCATAACCTTTATCTATGAGGTGGTTCAACCTCGTTGTTGAAAGAGTTTCATCCAGTTCATTGACTTTAGCTTCGCgt                 >  1:139925/1‑249 (MQ=255)
                                                                                                                                                                                                                                             ggCCATTCTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAATATTTGTAGTATAAATAACATTAAGTAAAGCCGCATCGGCTTCTCTGTAATGTTTATCTTTTTCCAGATAACCTGGGGATTCTCCCCCTAAATCAAGCTGTAACGTTATTCTTTCATAACCTTTATCTATGATGTTTTTCAACCTCGTTGTTGAAAGAGTTTCATCCAGTTCATTGACTTTAGCTTCTCGTAGTAAATCAGAGACa  <  2:308144/251‑1 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 
TTGCTTACGCCAGAGAAATAACTGGCTGGCTGCTACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTTCTCTGTAATGTTTATCTTTTTCCAGATAACCTGGGGATTCTCCCCCTAAATCAAGCTGTAACGTTATTCTTTCATAACCTTTATCTATGAGGTGGTTCAACCTCGTTGTTGAAAGAGTTTCATCCAGTTCATTGACTTTAGCTTCGCGTAGTAAATCAGAGACA  >  NC_000913/2997445‑2997932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.