Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	243,382	IS1 (+) +9 bp	intergenic (‑79/‑153)	fadE ← / → gmhA	acyl‑CoA dehydrogenase/D‑sedoheptulose 7‑phosphate isomerase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	19796 =	NA (NA)	64 (1.060)	57/482	0.1	100%	noncoding (768/768 nt)	IS1	repeat region
	?	NC_000913	243382 =	0 (0.000)					intergenic (‑79/‑161)	fadE/gmhA	acyl‑CoA dehydrogenase/D‑sedoheptulose 7‑phosphate isomerase
*	?	NC_000913	= 243390	0 (0.000)	65 (1.080)	59/482	0.1	100%	intergenic (‑87/‑153)	fadE/gmhA	acyl‑CoA dehydrogenase/D‑sedoheptulose 7‑phosphate isomerase
	?	NC_000913	= 258675	NA (NA)					noncoding (1/768 nt)	IS1	repeat region

TGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTC  >  NC_000913/258452‑258703                                                                                                                                                                                                                                |                             tGATTTTCTGGTGCGTAAAGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGAAGAGGTAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCCACAACCCGTCAGTAGCTGAACAGGGGGGCCAGCTGATAGCAAAAGAACCCACTGGAGCACCTCAAAACCCACATCATACCCTAAATCAGTAAGTTGGCAGCATC‑CCTGatacgccccttatcctggattatctg  >  2:458479/1‑226 (MQ=255)                                                                                                                                                                                                                                |                             TGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTC  >  NC_000913/258452‑258703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.