Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	2,238,267	IS1 (+) +9 bp	coding (1015‑1023/1521 nt)	mglA ←	D‑galactose/methyl‑galactoside ABC transporter ATP binding subunit

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1978503 =	NA (NA)	77 (1.280)	68/482	0.1	100%	noncoding (768/768 nt)	IS1	repeat region
	?	NC_000913	2238267 =	0 (0.000)					coding (1023/1521 nt)	mglA	D‑galactose/methyl‑galactoside ABC transporter ATP binding subunit
*	?	NC_000913	= 1979270	NA (NA)	44 (0.730)	39/482	0.5	100%	noncoding (1/768 nt)	IS1	repeat region
	?	NC_000913	= 2238275	0 (0.000)					coding (1015/1521 nt)	mglA	D‑galactose/methyl‑galactoside ABC transporter ATP binding subunit

CAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTG  >  NC_000913/1979044‑1979295                                                                                                                                                                                                                                   |                          cgatgatgTTCGGGTGCGTACGGGGTTGAGAAGCGGTGGTAGTGAGCTGCAGTGGTCAGGTTTTGCGGCAGGTAGAGCAGAGATGGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGTGCCTCCTCGTCAGTAGCTGAACAGGAGGTACAGCTGGCAGAAACAGAAGGCAATGGAGCACCTCAAAAACACCGTGATACACTAAATCAGTAAGTTGGCATTATCACCGATacgcgacttatccagtattat   <  2:193913/249‑21 (MQ=255)  attaaTTTTCTGGGGCGTACCGGGGTGCGAAGCGTTGTAAGTGACCTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGTCGTCAGGCGTTGCTTATGCAATGACGCACCAACCAGGCAGGCGCTGGACAGGAGGGACAGCTGATTGAAACAGAAACCCCGGGAGCACCTCAAAAACACATTCAAACACTAAATCCATAAGTTAGAAGCA‑CACCCGTaaacctcttgataattgtattt  >  2:543386/5‑231 (MQ=255)                                                                                                                                                                                                                                   |                          CAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTG  >  NC_000913/1979044‑1979295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.