Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 362,204 IS1 (–) +9 bp coding (968‑976/1254 nt) lacY ← lactose permease

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 19796 =NA (NA)63 (1.040) 56/482 0.1 100% noncoding (768/768 nt) IS1 repeat region
?NC_000913 = 362212 0 (0.000)coding (968/1254 nt) lacY lactose permease
* ? NC_000913 = 258675NA (NA)65 (1.080) 62/482 0.1 100% noncoding (1/768 nt) IS1 repeat region
?NC_000913 362204 = 0 (0.000)coding (976/1254 nt) lacY lactose permease

TGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTC  >  NC_000913/258452‑258703
                                                                                                                                                                                                                               |                            
tGATTTTCTGGTGCGTAAAGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGAAGAGGTAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCCACAACCCGTCAGTAGCTGAACAGGGGGGCCAGCTGATAGCAAAAGAACCCACTGGAGCACCTCAAAACCCACATCATACCCTAAATCAGTAAGTTGGCAGCATC‑CCTGatacgccccttatcctggattatctg  >  2:458479/1‑226 (MQ=255)
                                                                                                                                                                                                                               |                            
TGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTC  >  NC_000913/258452‑258703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.