Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,025,601 (T)5→4 coding (1251/1401 nt) xanQ → xanthine:H(+) symporter XanQ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,025,6010T.92.5% 152.2 / 6.6 40coding (1251/1401 nt)xanQxanthine:H(+) symporter XanQ
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base . (14/23);  total (16/24)
Fisher's exact test for biased strand distribution p-value = 5.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACGTTATGTCGGGCGAACCATCGCGGTAATGCTGGTTATCCTCGGCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTA‑TTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAACAGTAAAGGAGTCAATGATGTCAGGAGAACACACGTTAAAAGCGGTACGAGGCAGTTTTATTGATGT‑CACCCGTACGATCGATAACCCGGAAG  >  NC_000913/3025357‑3025844
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     
cccGTTTTGTCGGGCTAACCAGTGCGTTAATGCTGGTTATCCTCGGCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGGTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCATCGGTTTAAAGCGCCGTGAAACACTTATTGTCGCAACTTCTTTAGGTTTAGGTCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAatat                                                                                                                                                                                                                                                <  2:375540/249‑1 (MQ=255)
 aCGTTATGTCGGGCGAACCATCGCGGTAATGCTGGTTATCCTCGGCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATAtt                                                                                                                                                                                                                                               >  1:420379/1‑251 (MQ=255)
       tGTCGGGCGAACCATCGCGGTAATGCTGGTTATCCTCGGCTTATTTCCGATGATTGGCGGCTTCTTCACGATCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATAtt                                                                                                                                                                                                                                               <  2:395686/245‑1 (MQ=255)
       tGTCGGGCGAACCATCGCGGTAATGCTGGTTATCCTCGGCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATAtt                                                                                                                                                                                                                                               >  1:395686/1‑245 (MQ=255)
        gTCGGGCGAACCATCGCGGTAATGCTGGTTATCCTCGGCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGc                                                                                                                                                                                                                                         <  2:108861/250‑1 (MQ=255)
               gAACCATCGCGGTAATGCTGGTTATCCTCGGCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTAttt                                                                                                                                                                                                                                 <  1:36159/251‑1 (MQ=255)
                   cATCGCGGTAATGCTGGTTATCCTCGGCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGt                                                                                                                                                                                                                             <  2:188169/251‑1 (MQ=255)
                        cgGTAATGCTGGTTATCCCCGGCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTA‑TTAg                                                                                                                                                                                                                       <  1:14427/251‑1 (MQ=255)
                          ggaatgttggtttgccTCGGCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCTTCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTTTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACTATCCCGAAATTTT‑AAAATATTGCCAGCTTCTATTTATGTA‑TTAGtt                                                                                                                                                                                                                     <  2:438644/237‑1 (MQ=255)
                                cTGGTTATCCTCGGCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTA‑TTAGTTGAAAAc                                                                                                                                                                                                               >  1:631413/1‑251 (MQ=255)
                                cTGGTTATCCTAGGCTTATTTCCGATGATTGGCGGCTTCTTAACGACCATTCCCTCGGCAGATCTTGGAGGCGCAATGACGTTGATGTTTTCCCTTATTGCCATCGCCGGGATTCGCATCATCATCACCAACGGTTTAAAGAGCGCTGAAACACTTATTGTCGCAAATTCTTTAGAGTTAGGGCATAGCGTCTCCTACGAACCCGAAATTTT‑AAAATATTTCCAACCTTTATTTATGTAATTAGtgcaaa                                                                                                                                                                                                                 >  2:502560/1‑245 (MQ=255)
                                    ttctCCTCGGCGTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGATGCGCAATGACGTTGATGTTTTACATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACCCTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAA‑TATTGCCAGCCTATATTTCTGTA‑TTAGTTGaaagcaca                                                                                                                                                                                                            >  2:374143/4‑245 (MQ=255)
                                      aTCCGCGGCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCGTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTA‑TTAGTTGAAAACCCTAtt                                                                                                                                                                                                         <  1:405319/251‑1 (MQ=255)
                                             gCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTA‑TTAGTTGAAAACCCTATTTGTGCTg                                                                                                                                                                                                  <  1:639569/251‑1 (MQ=255)
                                                        atgatTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCGCCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTA‑TTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACt                                                                                                                                                                                       <  1:394588/251‑1 (MQ=255)
                                                               gcggcTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTA‑TTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAAATGCGAttt                                                                                                                                                                                >  1:227643/1‑251 (MQ=255)
                                                                         cACGGCCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTTATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAA‑GCGCCGTGAAACACTTATTGCCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTA‑TTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTATTAAatat                                                                                                                                                                      <  2:601864/250‑1 (MQ=255)
                                                                         cACGACCATTCCCCCGGCAGTTCTGGGAGGCGCAACGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTA‑TTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTATTAAata                                                                                                                                                                       <  1:15052/250‑1 (MQ=255)
                                                                          aCGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTA‑TTAGTTGAAAACCCTATTTGTGATGGCGGGTTAACTGCGGTTTTATTAAAtatt                                                                                                                                                                     >  1:585550/1‑251 (MQ=255)
                                                                            gACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTA‑TTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTATTAAAtattat                                                                                                                                                                   <  1:113438/251‑1 (MQ=255)
                                                                             aCCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGGTGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTA‑TTAGTTGAAAACCATTTTTTTGCTGGCGGGtgtaattcggttttttttaaattttt                                                                                                                                                                   >  2:160685/1‑226 (MQ=255)
                                                                                  tCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCATCGGTTTAAAGCACCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTA‑TTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTATTAAATATTAtcctcc                                                                                                                                                              <  1:45769/250‑1 (MQ=255)
                                                                                    ccTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAGTATTGCCAGCCTCTATTTATGTA‑TTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGGGTTTTTATTAAATATTATCCTccc                                                                                                                                                             >  1:300351/1‑249 (MQ=255)
                                                                                                                       ttttCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCACCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTA‑TTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTTTTAAATATTTTTCTCCCTGGGGGCCACCCAAAGGAAAACGTTCTTCCTGGTAtt                                                                                                                        >  1:419272/1‑251 (MQ=255)
                                                                                                                             aTGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACTCTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTA‑TTAGTTGAAAACCCTATTTGTGCTGGCGGTTTAACTGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGTTATTTCCTCa                                                                                                                  <  2:569648/251‑1 (MQ=255)
                                                                                                                                  tGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTA‑TTAGTTGAAAACCCTATTTGTGCTGGCGGGTTTACTGCCGTTTTATTTTATATTATTCTCACTTGTGGCTACCGACAGGAAAACGTTTTTGCTGGTTTTAACTCAGCggg                                                                                                             >  1:619879/1‑250 (MQ=255)
                                                                                                                                                   gCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTTCCAGCCTCTATTTATGTA‑TTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAACAGt                                                                                            <  1:661898/251‑1 (MQ=255)
                                                                                                                                                   gCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTA‑TTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTATTAAATATTATCCTCCCTGGTGGGTCCCGACAGGAAAACGTTTTGCCTGGTTTTACCCCAGCGGGAGAGATGGGTTAAAAGt                                                                                            >  1:147667/1‑251 (MQ=255)
                                                                                                                                                    catcatcatCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTA‑TTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAACAGTa                                                                                           <  1:443242/251‑1 (MQ=255)
                                                                                                                                                              cccAACGGTTTAAAGCGCCGTGAAACCCTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATAGTGCCAGCCTCTATTTATGTA‑TTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTATTAAATATTATCCTCCCTGGTGTCTACCGACAGGAAAACGATCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAACAGTAAAGGAGTCaa                                                                                 <  2:420379/250‑1 (MQ=255)
                                                                                                                                                                                  tGAAACCCTTATTGTCGCCACTTCGTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTA‑TTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCg                                                                                                                                                 <  2:513430/167‑1 (MQ=255)
                                                                                                                                                                                  tGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTA‑TTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAAATGCGATTTTTTTAAATATTATTCTCCCTGGGGGcgtccg                                                                                                                                                 >  1:513430/1‑162 (MQ=255)
                                                                                                                                                                                  tGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTA‑TTAGTTGAAAACCCTATTTGTGCTGGCGGGGTAAATGCGGTTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGAGGGATTAACAGGAAAGGAGTCAATGATGTCCGGAGAACAAACg                                                             >  1:197594/1‑251 (MQ=255)
                                                                                                                                                                                          ttATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTA‑TTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGGTTTTATTAAATATTATTCTCCCTGGGGGCTACCGACAGGAAAAAGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAAAAGTAAAGGGGTCAATGATGTCAGGAGAACACACGTTAAAAGc                                                     >  1:556726/1‑251 (MQ=255)
                                                                                                                                                                                                   ccACGTCTTTAGGTTTAGGGTTTGGCGGCTCCTACGATACCGAAATTTT‑AAAATATTGCCAGCCTCTATTTAAGTA‑TTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAACAGTAAAGGAGTCAAGGATGTCAGGAGAACACACGTTAAAAGCGGTACGAgg                                            <  2:220925/251‑1 (MQ=255)
                                                                                                                                                                                                          tttaggtttaggGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTA‑TTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTATTAAATATTATCCTCCCTGGTGGCTAc                                                                                                                                                   <  1:629146/141‑1 (MQ=255)
                                                                                                                                                                                                                           gCGTCTACTACGATCCCGAAATTTT‑AAAATAGTGCCATCCTCTATTTATGTA‑TTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGAGGGATTAACAGTAAAGGAGTCAATGATGGCAGGAGACCACACGTTAATAGCGGTACGAGGCAGTTTTATTGATGT‑CACCCGTAc                   <  2:235779/251‑1 (MQ=255)
                                                                                                                                                                                                                                  cgaCGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTAGGTA‑TTAGTTGAAAACCCTATTTTTGCTGGCGGGTTAACTGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTATTTCCTCAGCGGAAGAGATGGATTAACAGTAAAGGAGTCAATGATGTCAGGAGAACACACGTTAAAAGCGGTACGAGGCAGTTTTATTGATGT‑CACCCGTAcgatcgat            <  2:197594/249‑1 (MQ=255)
                                                                                                                                                                                                                                  cTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTA‑TTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTATTAAATATTTTCCTCCCTGGTGGCTACCGACAGGAAAAAGTTCTGCCTGGGATTACCTCAGCGGAAGAGATGGATTAACAGTAAAGGAGTCAATGATGTCAGGAGAACACACGTTAAAAACGGTACGGGGCAGGTTTATTTATGT‑CACCCGTAcgatcgat            >  1:139391/1‑251 (MQ=255)
                                                                                                                                                                                                                                           cGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTA‑TTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTATTAAATATTATCCTCCCTGGTAGCTACCGACAGGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAACAGTAAAGGAGTCAATGATGTCAGGAGAACACACGTTAAAAGCGGTACGAGGCAGTTTTATTGATGT‑CACCCGTACGATCGATAACCCGGa    <  1:160685/251‑1 (MQ=255)
                                                                                                                                                                                                                                               aattttAAAATATTGCCAGCCTCTATTTATGTA‑TTAGTTGAAAACCCTATTTGTGCTGGCGGGGTAACTGCGATTTTATTAAATATTATTCTCCCTGGTGGCTACCGAAAAGAAAAAGATCTGCCTGGTATTACCTCAGCGGGAGAGATGGATTAAAAGTAAAGGAGGCAAAGATGTCAGGAGAACAAACGTTAAAAAGAGTAAGAGGGAGGTTTTTTGGTGTTCACC‑GTACGATCGTTAACCCGGAag  >  1:397880/3‑249 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     
CACGTTATGTCGGGCGAACCATCGCGGTAATGCTGGTTATCCTCGGCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTA‑TTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAACAGTAAAGGAGTCAATGATGTCAGGAGAACACACGTTAAAAGCGGTACGAGGCAGTTTTATTGATGT‑CACCCGTACGATCGATAACCCGGAAG  >  NC_000913/3025357‑3025844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑