Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 310 (T)9→8 intergenic (+55/‑27) thrL → / → thrA thr operon leader peptide/fused aspartate kinase/homoserine dehydrogenase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133100T.97.0% 126.6 / ‑4.3 33intergenic (+55/‑27)thrL/thrAthr operon leader peptide/fused aspartate kinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base . (19/13);  minor base C (0/1);  total (19/14)
Fisher's exact test for biased strand distribution p-value = 4.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATT  >  NC_000913/74‑546
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                              
tGAACTGGTTACCTGCCGTGAGTAAATTAGAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGAGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAACCCGCACCTGACAGTGCGGGCTTTTTTTTCGACCAAAgtaaa                                                                                                                                                                                                                                   >  2:638676/1‑245 (MQ=255)
   actggtctccTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTAACTAAATACTTTAACCAATATAGGCATAGCGCACAGACATATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTCCCACAGGTAACGGTGCGGGCTGACGCATACAGTAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTCGACCAAAGGTAACGAgg                                                                                                                                                                                                                              <  2:168758/243‑1 (MQ=255)
   aCTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTCGACCAAAGGTAACGAgg                                                                                                                                                                                                                              >  1:117738/1‑251 (MQ=255)
               ccGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGCCAGTGCGGGCTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGc                                                                                                                                                                                                                  >  2:300529/1‑251 (MQ=255)
                               aTTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTCGACCAAAGGAAACGAGGTAACAACCATGCGAGTGTTGAAGTTcgg                                                                                                                                                                                                  >  1:98042/1‑251 (MQ=255)
                                 tttATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGGACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTcggc                                                                                                                                                                                                 <  2:570276/250‑1 (MQ=255)
                                  ttATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCGTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTcggcg                                                                                                                                                                                                <  1:430861/250‑1 (MQ=255)
                                       gACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACAt                                                                                                                                                                                          <  1:300529/251‑1 (MQ=255)
                                               tCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGCTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCCCCACCATTACCACCACCATCACCATTACCACAGGTACCGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCCTTTTTTTCGACCAAAGGTAACGAGGTAACACCAATGCGAGAGTTGATTTTCGGCGGTACATCAGTGGcg                                                                                                                                                                                  >  2:236283/1‑250 (MQ=255)
                                                    aactACTTTAACCAATATAGGCATAGCGCACAGAACGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGAGCGGGCTGACGCGTAAAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTACGACCAAAGGTAACGAGGTAACAACAATTCGCGAGTTGAAGTACGGCGGGAAATCAGGGGCAAAtac                                                                                                                                                                             >  2:629068/4‑249 (MQ=255)
                                                     aaTACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGc                                                                                                                                                                             >  1:158704/1‑250 (MQ=255)
                                                             aaCCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGttt                                                                                                                                                                    <  1:641063/251‑1 (MQ=255)
                                                             aaCCAATAAAGGCATAGCGCCAATAACAATAAAAATTTCAGAATTCCCAACATCCATGAAACGTATTAGCACCCCCCATACCCCCACCATCCCCAATACCACAGGTAACGGTGGGGGCTGGAGCGGTCAGGAAACACAGAAAAAAGCCCGGCCCTGACAGTTCGGGCTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTGCGGCGGTACATCAGTGGCAAATGCAGAACGttt                                                                                                                                                                    <  2:98042/251‑1 (MQ=255)
                                                                                                     aaagTACAAAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGCGCTGACGCGTACAGGAAACACAGAAAAAAGCACGCACATGACAGTGAGGACTTATTTTTCGACCAAAGGGAACGAAGTAACAACCATGAGAGGGTTGAGGTTAGCAGGGACAAAAGTAGCAACTGCAGAACGTTTGCTGTGGGTTGACGAAATTCTGGaaaagcattgcaggc                                                                                                                              >  2:433329/3‑238 (MQ=255)
                                                                                                        gTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAggcaggg                                                                                                                          >  1:518965/1‑251 (MQ=255)
                                                                                                             caacaTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAggcaggggcagg                                                                                                                     >  1:255277/1‑251 (MQ=255)
                                                                                                             caacaCCCATGAAACGCATTAGCACCACCATTACCACCACCAGCACCATTACCACAGGTAACGGGGCGTGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAggcaggggcagg                                                                                                                     <  2:518965/251‑1 (MQ=255)
                                                                                                               acaTCCATGAAACGCATTAGCACCACCATGACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGAGCGGGCTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGGACATCAGTGGCAAATGAAGAACGTTTTATGTGGGTTGTCGCGATTCTCGAAAGCAATGcctggaagtgagagggg                                                                                                                   >  2:471157/1‑236 (MQ=255)
                                                                                                                           cGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGAGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCctc                                                                                                      >  1:23601/1‑251 (MQ=255)
                                                                                                                                           ttACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCaaa                                                                                       >  1:355054/1‑250 (MQ=255)
                                                                                                                                                         cccactACCACAGGTAACGGTGCGGGCTGACGCTGCCGGGAAACACTAAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTGGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGCCAGGTGGCACCGGCCCTCTCTGCCCCCGCCAAAATCACCAACCACCtg                                                                       <  2:122079/246‑1 (MQ=255)
                                                                                                                                                                                   cTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCccca                                                                               >  2:183687/1‑217 (MQ=255)
                                                                                                                                                                                   cTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCacca                                                                               <  1:183687/217‑1 (MQ=255)
                                                                                                                                                                                          gTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGcc                                        >  1:270585/1‑249 (MQ=255)
                                                                                                                                                                                          gTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAg                                      <  1:535731/251‑1 (MQ=255)
                                                                                                                                                                                             cAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCAGGCTTTTTTTTCGACCAAAGGAAACGGGGTAACAACCATGCGATTGTTGAAGTTCGGCGGTACATCCGTGGCAAATGCAGCACGTTTTCTGCGGGTTGTCGATATTCTGGAAAGCAATGCCAGGCAGGGGCCGGGGGCCGCCGCCATCTCTCCCCCCGCCACAATCACCAACCACCTGGGGGCGGTGATTGAACAAACCATTacagcccagggt                                   >  1:327687/1‑240 (MQ=255)
                                                                                                                                                                                                     acaGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTAcc                           <  1:638676/251‑1 (MQ=255)
                                                                                                                                                                                                     aaaaaaaaaaACCCCCACCTTAACAGTGGGGGTTTTTTTTCCGACCAAAGTTAAAGCGGTAACAACCATGCGAGGGTGTAAGTTCGGCGGTACATCAGTGGCAAATGCAGACCGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTAc                            <  2:158704/247‑1 (MQ=255)
                                                                                                                                                                                                      caaaaaaaaGCCCGCCCCGGACGGTGCGGGCTTTTTTTTCTACCAAAGTTAGCGAGGTAACAACCGTGCGAGTGTGGAAGATGGGCGGTACATCAGTGGCAAGTGCAAACCGTTCTCCGCGGGTTGCCGATATCCTGGAAAGCAAGCCCAGGCAGGGGCGGGTGGCCACCGTCCTCGCTACCCCCGCCAAAATCACCAACCACCTGGTGTCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTAccc                          <  2:310760/248‑1 (MQ=255)
                                                                                                                                                                                                       aGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTAcccc                         >  1:463083/1‑250 (MQ=255)
                                                                                                                                                                                                       aGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCa                         <  1:503385/251‑1 (MQ=255)
                                                                                                                                                                                                                  cGCAACTGACAGTGCGGGCTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGAGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCCGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATACACAACCACCTGGTGGCGATGCTTGAAAAAACCATTAACGGCCCGGATACTTTACCCCATATCAGCGAt              >  2:587779/1‑251 (MQ=255)
                                                                                                                                                                                                                               tGCGGGCTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTAtt  >  1:12157/1‑251 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                              
TGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATT  >  NC_000913/74‑546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: