Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,111,727	2 bp→TT	coding (865‑866/2544 nt)	opgH →	osmoregulated periplasmic glucans biosynthesis protein H

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,111,727	0	C	T	94.1%	102.4 / ‑1.8	35	P289M (CCG→TTG)	opgH	osmoregulated periplasmic glucans biosynthesis protein H
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base T (18/14);  total (20/15)
Fisher's exact test for biased strand distribution p-value = 5.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01
*	NC_000913	1,111,728	0	C	T	94.3%	106.9 / ‑3.4	35	P289M (CCG→TTG)	opgH	osmoregulated periplasmic glucans biosynthesis protein H
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base T (18/15);  total (20/15)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGACGGCGTTAATGGGCTTCCTGCAACTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTG‑GCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCC  >  NC_000913/1111491‑1111962                                                                                                                                                                                                                                              ||                                                                                                                                                                                                                                           ggACGGCGTTAATGGGCTTCCTGCAACTGCTTATTGGTCGCGATAAATACAGTATTTCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTG‑GCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTc                                                                                                                                                                                                                               >  1:133427/1‑251 (MQ=255)        gTTAATGGGCTTCCTGCAACTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTG‑GCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGc                                                                                                                                                                                                                        <  1:225493/251‑1 (MQ=255)                                tATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTG‑GCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATc                                                                                                                                                                                                 <  2:142176/250‑1 (MQ=255)                                     gTCGCGATAACTACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACACGTAGCATCGCACGGCGTTGTTCATGCCTATCTGTAACGAAGACGTGAACCGTGGTTTTGCTG‑GCCTGCGTGCAACGTGGGAATCAGTACAAGCCACCGGGAATGCCAAACACTCTGATGTATACTTTCTTAGTGACAGTAATAACCCGGATAACAGCGTCGCAGCGCACAACGCGTGGATGGAGCTTATCGCTgc                                                                                                                                                                                            >  2:43437/1‑249 (MQ=255)                                      tCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTG‑GCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGa                                                                                                                                                                                            >  2:208759/1‑249 (MQ=255)                                              aaTACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTG‑GCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGc                                                                                                                                                                                  <  2:94791/251‑1 (MQ=255)                                              aaTACAGTATATCTGCGGCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTG‑GCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGc                                                                                                                                                                                  <  2:184286/251‑1 (MQ=255)                                                           tGCGTCAACAGTTGGCGATGCACCATTAAACCCGGAGCATCGCACGGCGTTAATCATGCCTCTCTGTAACGAAGCCGTGCACCGTGTTTTTGCTG‑GCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGCTGTCTACCTTCTTAGTGACAGTAATAACTTGGATATCTGCGTCGCAGAACAAAAAGCCTGGTTGGAGCTTATCGCGTAAGTCGGTGGCGAAGTGCAGAttt                                                                                                                                                                     >  2:221559/1‑251 (MQ=255)                                                             cGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTG‑GCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGGGGCGCAGGTCAGATTTTc                                                                                                                                                                   >  1:62466/1‑251 (MQ=255)                                                                 aaCAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTG‑GCCTGCGTGCAACGTGGGAATCCGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCACAAAGCCTGGATGGAGCTTATCGCTGAAGTAGGTGGCGAAGGTCCGATTTTCTATc                                                                                                                                                               >  2:369618/1‑251 (MQ=255)                                                                  aCAGTTGGCGATGAACCCTTAAACCCGGAGCATCGCAAGGCGTTGATCATGCCTATCTGCAACGACGACGTGAACCGTGTTTTTGCTG‑GCCTGCGTGCAACGTGGGAATCAGTAAAAGACACCGGGAATGCCAAACACTTTGATGTCTACATTCTTGGCGACAGTTATAACTTGGATATATGCGTCGAAGACCAAAAGGCCTGGCTGGCGCTAATCGCTGAAGTCGGTGGCGAAGGTCCGCTTTTCTATcg                                                                                                                                                              >  2:350793/1‑251 (MQ=255)                                                                   cagatGGCGATGAACCATTAAACCCGGGGCCTCGCACGGAGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTG‑GCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGACGGTCAGATTTTCTATcgc                                                                                                                                                             >  1:295963/5‑251 (MQ=255)                                                                   cAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTG‑GCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATcgc                                                                                                                                                             >  1:295953/1‑251 (MQ=255)                                                                     gTTGGCGATGAACCATTAAACCCGGAGTATCGCACGGCGTTGATCATGCCTAGCTGTAACGAAGACGTGAACCGTGTTTTTGCTG‑GCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCGTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATcgccg                                                                                                                                                           <  2:249728/251‑1 (MQ=255)                                                                       tGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTG‑GCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATcgccgcc                                                                                                                                                         >  1:238731/1‑251 (MQ=255)                                                                                      aaaCCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTG‑GCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGACGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTg                                                                                                                                           >  1:20490/1‑250 (MQ=255)                                                                                                   cGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTG‑GCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCgg                                                                                                                             >  1:248631/1‑251 (MQ=255)                                                                                                         gCGTTGATCATGCCTATCTGTAACGAAGACTTGAACCGTGTTTTTGCTG‑GCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAatat                                                                                                                       <  2:324198/251‑1 (MQ=255)                                                                                                                        aTCTGTAACGAAGACGTGAACCGTGTTTTTGCTG‑GCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCg                                                                                                        >  1:206771/1‑251 (MQ=255)                                                                                                                              aaCGAAGACGTGAACCGTGTTTTTGCTG‑GCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGTAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGAGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCt                                                                                                   <  1:221559/250‑1 (MQ=255)                                                                                                                                  aaGACGTGAACCGTGTTTTTG‑TGCGCCTGCGTGCAACGTGTGAATTAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTATGGACAGGTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCCGGATTGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAATCGGTAATATCGATGACTTCTGCCGTCGCTGGGGc                                                                                              <  2:310987/251‑1 (MQ=255)                                                                                                                                     aCGTGAACCGTGTTTTTGCTG‑GCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGAAAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGGCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGACGCGTAAAAGCGGTAATATCGAAGACTTCTGCCGTCGCTGGGGCAGc                                                                                           >  2:307901/1‑251 (MQ=255)                                                                                                                                                    ttGCTG‑GCCTGCGTTCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTTCATTCTTAGTGACAGTTATAACTTGGATACCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCTGTCGCTGGGGCAGCCAGTACAGCTACAt                                                                             <  1:379275/250‑1 (MQ=255)                                                                                                                                                         tggccatcgtgaaccGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGACTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTGCTATCGCCGCCGCCGGCGCCGCGTGAAGCGTAAAAGCGGTAATATCGAGGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACAtggtg                                                                         <  2:317194/236‑1 (MQ=255)                                                                                                                                                         g‑gCCTGCTGGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATggtggt                                                                       <  1:208965/251‑1 (MQ=255)                                                                                                                                                           gCCTGCTTGCAACGTGTGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTGGATGTCTACATGCCTAGGGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCGGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGgtggtg                                                                      <  2:73627/251‑1 (MQ=255)                                                                                                                                                               gCGTGCAACGTGGGAATCAGTAAAAGCCACCGGTAATGCCAAACCCTTTGATGTCTACATTCTTAGTGACAGTTATAACGTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTgg                                                                  <  1:345224/251‑1 (MQ=255)                                                                                                                                                                     aaCGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTg                                                            >  1:379731/1‑251 (MQ=255)                                                                                                                                                                              aTCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACCGCTACATGGTGGTGCTGGATGCTGACGCGGTa                                                    >  2:145185/1‑250 (MQ=255)                                                                                                                                                                                           aCCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGAttg                                      <  1:217987/251‑1 (MQ=255)                                                                                                                                                                                                     ccAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGCAGCGTAAAAGCGGTAATCTCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGCAATGACCGGTGATTGTTTGTGCggg                            >  1:176105/1‑251 (MQ=255)                                                                                                                                                                                                                       aCATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGa           >  1:268809/1‑250 (MQ=255)                                                                                                                                                                                                                            cTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGCGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCAGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCa      >  1:249180/1‑250 (MQ=255)                                                                                                                                                                                                                              tAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAACGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAAcc   >  1:327790/1‑251 (MQ=255)                                                                                                                                                                                                                               aGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAAccc  <  2:205756/251‑1 (MQ=255)                                                                                                                                                                                                                                              ||                                                                                                                                                                                                                                           GGACGGCGTTAATGGGCTTCCTGCAACTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTG‑GCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCC  >  NC_000913/1111491‑1111962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑