Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	365,306	G→A	R334C (CGC→TGC)	lacZ ←	beta‑galactosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	365,306	0	G	A	100.0%	15.5 / NA	7	R334C (CGC→TGC)	lacZ	beta‑galactosidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/4);  total (3/4)

CGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTC‑GCGGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTCACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCACCCTGCCATAA  >  NC_000913/365133‑365525                                                                                                                                                                               |                                                                                                                                                                                                                            cGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTC‑GCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCAAGAtgggaat                                                                                                                                                >  2:82528/1‑245 (MQ=255)                                                                cTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACTGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTC‑GCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTCGTGAGACGCGATCgg                                                                                 >  1:55859/1‑251 (MQ=255)                                                                                      cgtggATGATGCTCGGGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCCCCTC‑GCAGAAACCGACAGCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGAt                                                           <  2:152486/248‑1 (MQ=255)                                                                                        agGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTC‑GCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAaccacc                                                                                                                                                             >  2:332304/1‑151 (MQ=255)                                                                                        agGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCGC‑GCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAaccacc                                                                                                                                                             <  1:332304/151‑1 (MQ=255)                                                                                                                 ccTCGAATCAGCAACGTCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCCCCTC‑GCAGAAACCTACATCGCAGGCTTCTGCTTCAATCAGCGGGCCGTCGGGGGTGTGCATTTCAACCACCGCACGAGGGAGATTCGGGATGTCGGCGCTCCACATTTTCGGGTTTTCGACGTTCAGACGTAGTGCGACGCGATCGGCATAACCACCACGCTCACCGATAATTTCACCGCCGAAAGGCGCGGTGcc                                <  2:37970/251‑1 (MQ=255)                                                                                                                                               ggcagaagcccATTTTCAATCCGC‑CCTCCGAAGAAACCGACAACGCAGGCTTCTGCTTCAATCAGCGGGCCGTCGGCGGTGTGCAGTTCAACCCCCGCCGGATAGAGATCCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTCACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCACCCTGCCATaa  <  1:82528/242‑1 (MQ=255)                                                                                                                                                                               |                                                                                                                                                                                                                            CGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTC‑GCGGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTCACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCACCCTGCCATAA  >  NC_000913/365133‑365525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑