Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,220,685	A→G	F601F (TTT→TTC)	arpA ←	regulator of acetyl CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,220,685	0	A	G	90.0%	23.2 / ‑5.3	10	F601F (TTT→TTC)	arpA	regulator of acetyl CoA synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (5/4);  total (5/5)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTTGTGGGGCTTTGTGTTGCCAAAAATCCATAATATCTTCAGCGGTAAATCCAAACAGGCGTGCATGGTCAGATAAAGCAAGATAAACCGTCTCTACAACATTTTGTTGTTTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTTATCGAATTTAAACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGT  >  NC_000913/4220479‑4220901                                                                                                                                                                                                               |                                                                                                                                                                                                                         ttttGTGGGGCTTTGTGTTGCCAAAAATCCATAATATCTTCAGCGGTAAATCCAAACAGGCGTGCATGGTCAGATAAAGCAAGATAAACCGTCTCTACAACATTTTGTTGTTTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTTATCGAATTTAAACGTATTGAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCAt                                                                                                                                                                              >  1:71967/1‑251 (MQ=255)       ggggCTTTGTGTTGCCAAAAATCCATAATATCTTCAGCGGTAAATCCAAACAGGCGTGCATGGTCAGATAAAGCAAGATAAACCGTCTCTACAACATTTTGTTGTTTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTTATCGAATTTAAACGTATTGAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTcc                                                                                                                                                                        <  1:169946/251‑1 (MQ=255)                                               tAAATCCAAACAGGCGTGCATGGTCAGATAAAGCAAGATAAACCGTCTCTACAACATTTTGTTGTTTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTTATCGAATTTAAACGTATTGAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCAATAAACAAACCCGTGTCGCGCGCa                                                                                                                                   >  2:259166/1‑248 (MQ=255)                                                           ggCGTGCATGGTCAGATAAAGCAAGATAAACCGTCTCTACAACATTTTGTTGTTTATGCTGGATCGCTGAGAACAAACCGGGATATTCATTAGAATTATTTGACAGGAAGCAGTGCTTCATATTTTTTTTATCGAATTTAAACGTATTGAACAGAGTGGATAATGCGTTAAAAATAGGATTAATAACGTTCATGGGTCCACGCTGCATGGCCATAACCAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTc                                                                                                                    >  1:198940/1‑251 (MQ=255)                                                                                 aaGATAAACCGTCTCTACAACATTTTGTTGTTTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGATGGGCTTCATATTTTTTTTATCGAATTTAAACGTATTGAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAg                                                                                              <  2:347954/251‑1 (MQ=255)                                                                                       aaCCGTCTCTACAACATTTTGTTGTTTATGCGGTAGCGCTGAAAACAAACCGGGATATTCATTAGAATTAGTTGCCAGGAGGAGGGGCTTCATATTTTTTTTATCGAATTTAAACGTATTGAACAGATGGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATAGCGGAAGCTGaaa                                                                                        <  2:92860/251‑1 (MQ=255)                                                                                        aCCGTCTCTACAACATTTTGTTGTTTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTTATCGAATTTAAACGTATTGAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGaaa                                                                                        >  2:205647/1‑250 (MQ=255)                                                                                            gctatacaaaaatttGGTGTTTATGCGGGATTGCGGAAAACAAACTGGGATATGAATGAGAATTATTTGCCAGGAGGGGGGGCTTCATATTTTTTGTAACGAATTTAAACTTATTGAACAGAGTGGGCCATGCGTTAAAAATAGTATTAATAACGCTCATGTGTACGAGCTGCCTGGCAATAAAAAAACCCGTGTCGCCCGCAAGCCTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTa                                                                                   <  2:198940/239‑1 (MQ=255)                                                                                                                                                                 ccAGGAGGAGGGGCTTCATATTTTTTTTATCGAATTTAAACGTATTGAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAAGGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCAAGATATCGCTATGTCCTTTTTGCATc              >  2:294137/1‑251 (MQ=255)                                                                                                                                                                             gCTTCATATTTTTTTTATCGAATTTAAACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGt  <  1:344656/251‑1 (MQ=255)                                                                                                                                                                                                               |                                                                                                                                                                                                                         TTTTGTGGGGCTTTGTGTTGCCAAAAATCCATAATATCTTCAGCGGTAAATCCAAACAGGCGTGCATGGTCAGATAAAGCAAGATAAACCGTCTCTACAACATTTTGTTGTTTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTTATCGAATTTAAACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGT  >  NC_000913/4220479‑4220901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑