| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,182,782 | A→T | Q513L (CAG→CTG) | rpoB → | RNA polymerase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,182,782 | 0 | A | T | 92.7% | 167.3 / 3.4 | 55 | Q513L (CAG→CTG) | rpoB | RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base A (1/3); new base T (26/25); total (27/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.94e-01 | |||||||||||
GGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGT‑CTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGT‑CCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGA > NC_000913/4182556‑4183027 | ggTAAAGGGGAAGTCGATGGGATCGACCACCTCGGCAACCGTCGTATCCGTTCCGGTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCTTGGTACGGTTAGAGCGGTCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCGGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCTGTTTATGGACCAGAACAACCCGcc < 2:306835/251‑2 (MQ=255) aGGCGAAGTCGATGAGATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCTGTTTATGGACCAGAACAACCCGCTGT‑CTg < 1:11446/251‑1 (MQ=255) aaGTCGATGAGATCGACGACCGCGGCAAGCGTCGTATCCGTGCCGTTGGCGAAATGGCGGAAAACCAGTTCTGCGTTGGACTGGTACGTGTAGAGCGTGCGTTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGAGATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCTGTTTATGGACCAGAACAACCCGCTGT‑CTGAGAtt < 1:262184/251‑1 (MQ=255) atCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCTGTTTATGGACCAGAACAACCCGCTGT‑CTGAGATTACGCACAAAcg < 1:164444/251‑1 (MQ=255) cccTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCTGTTTATGGACCAGAACAACCCGCTGT‑CTGAGATTACGCACAAACGTCGTAtc < 1:172927/250‑1 (MQ=255) ggccaCCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGCGCGGTGCAAGCGCGTCTGTCTCTGGGCGATCTGGATACCATGATGCCACAGGATATGATCAACGCCAAGCAGATTTCCGCAGCAGTGAACGAGTTATTCGGTTCCAGCCAGCTGTCTCTGTTTATGGACCAGGACCACCCGCTGT‑CTGAGATTACGCACAAACGTCGTCTCACCGc > 2:64480/5‑251 (MQ=255) ccGTTGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTGCCGCGTTGGCCTGGTACGTGTAGGGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCTGTTTATGGACCAGAACAACCCGCTGT‑CTGAGATTACGCACAAACGGCGTATCTCCGCACTCg < 2:188518/251‑1 (MQ=255) ccGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTGCCGCGTTGGCCTGGTACGTGGAGAGCGTGCGGTGAAAGAGCGTCTTTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCTGTTTATGGACCAGAACAACCCGCTGT‑CTGAGATTACGCACAAACGTCGTATCTCCGCACTCg < 2:26978/251‑1 (MQ=255) gtATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCTGTTTATGGACCAGAACAACCCGCTGT‑CTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGccc > 1:26308/1‑250 (MQ=255) ccgttccgttGGCCGAATGTCGGAAACCCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCTGGCGTTGAAAGAGCGTCTGTATCTGGGCGATCTGGACACCCTGATGCCACAGGATATTATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCTGTTTATGGACCAGAACAACCCGCTGT‑CGGAGATTACGCTCAAACGTCGTATCTCCGCATTCGCCCCAGGCg < 2:273429/251‑1 (MQ=255) gttccgttGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCTGTTTATGGACCAGAACAACCCGCTGT‑CTGAGATTACGCCCAAACGTCGTATCTCCGCACTCGGCCCcggcggg > 2:64568/1‑250 (MQ=255) cgttGGCGGAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCTGTTTATGGCCCAGAACAACCCGCTGT‑CTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAAGCgggcatc > 2:231143/1‑246 (MQ=255) cgttGGCGAAAGGGCGGAAAAACAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGAGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGACGATTTCCGCAACAGTGAAAGAGATCTTCGGTTCAAGCCAGCTGTCTCTGTTTATGGACCAGAACAACCCGCTGC‑CTGAGATTACGCACAAATGTCGTATCTCCGCAATCAGCCCAGGGGGTCTgc > 2:231137/1‑250 (MQ=255) gggAAAACCAGGTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGGGCGTCTGGCTCTGGGCGATCTGGATCCCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCTGTTTATGGACCAGAACAACCCGCTGT‑CTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCa < 2:40589/250‑1 (MQ=255) ggAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCTGTTTATGGACCAGAACAACCCGCTGT‑CTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAg < 1:355656/251‑1 (MQ=255) gcgTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCTGTTTATGGACCAGAACAACCCGCTGT‑CTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCga < 1:72308/251‑1 (MQ=255) cgTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCTGTTTATGGACCAGAACAACCCGCTGT‑CTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCg > 1:333749/1‑249 (MQ=255) tGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCTGTTTATGGACCAGAACAACCCGCTGT‑CTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACg > 1:214059/1‑251 (MQ=255) tGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCTGTTTATGGACCAGAACAACCCGCTGT‑CTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGCCTGACCCGTGAACGTGCCGGCTTCGAAGTTCggaacg > 2:133790/1‑246 (MQ=255) tGGCCTGGTACGGGTAGAGCGTGCGGTGAAAGTGCGTCTGTCGCTGGGCGATCTGGAGGCCCGGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCTGTTTATGGACCAGAACAACCCGCTGT‑CTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGGCCCGTGAACGTGCAGGCTTCGAAGGTCGAGAc < 2:170012/250‑1 (MQ=255) ggCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCTGTTTATGGACCAGAACAACCCGCTGT‑CTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGt > 2:240105/1‑251 (MQ=255) ggTACGTGTAGAGCGTGCGGTGAGAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTACAGCCAGCTGTCTCTGTTTATGGACCAGAACAACCCGCTGT‑CTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTAGACGTTCGAGACGGACAcc > 2:326722/1‑251 (MQ=255) acgggtAGAGCGGGCGGTGAAAGAGCGTCTGTCTCGGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCTGTTTATGGACCAGAACAACCCGCTGT‑CTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCg < 2:347723/246‑1 (MQ=255) aagCGTGCGGTGAAAGAGCGTTTGTCTCTTGGCTAACTGGATACCCTGAGGCCACAGGATATGAAAAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGT‑CTGAGATTACGCACAAACGTCGTATCTCCGCCCTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGATACGTACCCCCGACCCACTa < 2:29125/250‑1 (MQ=255) agCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCTGTTTATGGACCAGCACAACCCGCTGT‑CTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCCAGCCGTACACCCGACTccctcc > 2:340023/1‑246 (MQ=255) cGTGCGGTGAAAGGGCGTCTTCCTCTGGGCGATCTGGATACCCTGATGCCACAGGTTATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCTGTTTATGGACCAGAACAACCCGCTGT‑CTGAGATTACGCACAAACGTCGTAGCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACgg < 2:330427/251‑1 (MQ=255) tGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCTGTTTATGGACCAGAACAACCCGCTGT‑CTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGCCGTACACCCGACTCACTACGGGCGCGTATGT‑CCAATCGAAAccccc > 2:312118/1‑250 (MQ=255) ggggCGATCTGGATACCCTGAGGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCTTTTTATGGACCAGAACAACCCGCTGT‑CTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGT‑CCAATCGAAACCCCt < 1:1278/250‑1 (MQ=255) gATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCTGTTTATGGACCAGAACAACCCGCTGT‑CTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTAGGT‑CCAATCGAAACCCCTGAAgg > 2:63162/1‑251 (MQ=255) gATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCAGATTTCCGCAGCAGTGAAAGAGTTCTTCGATTCCAGCCAGCTGTCTCTGTTTATGGACCAGAGCAACCCGCTGT‑CTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTAACCCGTGACCGTTCAGACTTCGAAGTTCGAGCCAGACACCCGACTCACTACCGTCGCGTA‑GTACCAAACGAAACCCCTgcac > 2:83865/1‑247 (MQ=255) ggATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCTGTTTATGGACCAGAACAACCCGCTGT‑CTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGT‑CCAATCGAAACCCCTGAAGGt < 1:267198/247‑1 (MQ=255) aTACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCTGTTTATGGACCAGAACAACCCGCTGT‑CTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGT‑CCAATCGAAACCCCTGAAGGTCCGAAc > 1:223871/1‑251 (MQ=255) aTACCCTGATGCCACACGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCTGTTTATGGACCAGAACAACCCGCTGT‑CTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGACCGTGCAGGCTTCGAAGTTAGAGACGTTCACACGACTCACTACGGTCGCGGATGT‑CCCACCGAAACCCCCGAAGGTCCGacc > 2:188380/1‑249 (MQ=255) actctgttgacacaGGATATGATTAACGCCAAGCAGATTTCCGCAGTAGTTAAAGAGTTCTCCGGTGCCAGCCAGCTGTCTCTGTTTATGGACCAGAACAACCCGC‑GTGCTGCGATTAATCAAAAACGTCGTATCGTCGCATTCGGCTCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCTAGACGTTCTCCCGTCTCACTACGGGCGCGTATGT‑CCAATCGAAACCCCTGAAGGTCCTAACa < 2:162167/240‑1 (MQ=255) aCCCTGATGCCACAGGATATGATCAACGCCATGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCTGTTTATGGACCAGAACAACCCGCTGT‑CTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGT‑CCAATCGAAACCCCTGAAGGTCCGAACAt < 1:150089/251‑1 (MQ=255) aCCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCTGTTTATGGACCAGAACAACCCGCTGT‑CTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGT‑CCAATCGAAACCCCTGAAGGTCCGAACAt > 1:168982/1‑251 (MQ=255) aCCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGT‑CTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGT‑CCAATCGAAACCCCTGAAGGTCCGAACa < 1:149513/250‑1 (MQ=255) cccTGATGCAACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTGCTCCGGTTCCAGCCAGCTGTCTCTGTTTATGGACCGGAACAACCCGCTGT‑CTGAGATTTCGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGCCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGT‑CCAATCGAAACCCCTGAAGGTCCGAACATc < 2:119539/251‑1 (MQ=255) acaGTATATTATCAACGACAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCTGTTTATGGACCAGAACAACCCGCTGT‑CTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGT‑CCAATCGAAACCCCTGAAGGTCCGAGCATCAGTCTGATCa < 1:319045/251‑1 (MQ=255) atGATCAACGCCAAGCCGATTCCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTATCTGTTGATGGACCAGAACAAACCGCTGT‑CTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCCGACCCGTGACCGTGCAGGCTTCGATGTTCGAGACGTACACCAGACTCACTACGGTCGCGTCTGT‑CCACTCGAAACCCAGGAAGGTCCGAACCTCgtgcagatcacctccac > 2:376172/1‑235 (MQ=255) aTCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCTGTTTATGGACCAGAACAACCCGCTGT‑CTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGT‑CCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTc > 1:351637/1‑251 (MQ=255) aTCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCTGTTTATGGACCAGAACAACCCGCTGT‑CTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGT‑CCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTc > 1:185036/1‑251 (MQ=255) cAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCTGTTTATGGACCAGAACAACCCGCTGT‑CTGAGATTACGCACAACCGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGCCTCACTACGGTCGCGTATGT‑CCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCACTGACCGTGTACg > 2:235405/1‑251 (MQ=255) cGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCTGTTTATGGACCAGAACAACCCGCTGT‑CTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGT‑CCAATCGAAACCCCTGCAGATCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGccc > 1:275068/1‑247 (MQ=255) gATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCTGTTTATGGACCAGAACAACCCGCTGA‑CTGAGATTCCGCACAAACGTCGTATCTCCGCAATCGGCCCAGGCGGTCTGACCCGTGAACGTGCAAGCTTCGAAGTTCGAGACGTCCACCCGACTCACAACGGTAGCGTATTT‑CCAATCGACACCCCTGACGGACCGAACCTCGGTCTGATCCACTCTCTGTCCGTGTACCcacaac > 2:328424/1‑249 (MQ=255) ccgtagcagTGAAAGAGTTGTTCGGTTCCAGCCAGCTGTCTCTGTTTATGGACCAGAACAACCCGCTGT‑CTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACCCCCGACTCACTACGGTCGCGTATGT‑CCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTaa < 1:233709/246‑1 (MQ=255) aaGAGTTCTTCGGTTCCAGCCAGCTGTCTCTGTTTATGGACCAGAACAACCCGCTGT‑CTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGT‑CCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGGGTACGCACAGACTAACGAATACGGCTTc > 2:127218/1‑251 (MQ=255) agagTTCTTCGGTTCCAGCCAGCTGTCTCTGTTTATGGACCAGAACAACCCGCTGT‑CTGAGATTACGCACACACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGACGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGT‑CCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCCCCGCCTAACGAATACGGCTTcc > 2:48148/1‑251 (MQ=255) gagTTCTTCGGTTCCAGCCGGCTGTCTCTGTTTATGGACCAGAACAACCCGCTGT‑CTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGT‑CCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCt < 2:214059/251‑1 (MQ=255) gagTTCTTCGGTTCCAGCCAGCTGTCTCTGTTTATGGACCAGAACAACCCGCTGT‑CTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGT‑CCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCt < 1:240105/251‑1 (MQ=255) tttcttcGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGACCAACCCGCTGT‑CTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTTAACGTGCAGGCTTCAAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGT‑CCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCtt < 2:128605/249‑1 (MQ=255) ttcttcGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGT‑CTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCAAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGT‑CCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCtt > 1:128605/1‑249 (MQ=255) gcGGTTCCAGCCAGCTGTCTCTGTTTATGGACCAGAACAACCCGCTGT‑CTGAGATTACGCACTAACGTCGTATCTCCGAGCTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGTGTATGT‑CCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACACACAGACTAACGAATACGGCTTCCTTgag < 1:120273/247‑1 (MQ=255) ccagccagCTGTCTCTGTTTATGGACCAGAACAACCCGCTGT‑CTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGCAGTTCGAGCCGTACACCCGACTCACAACGGTCGCGTATGT‑CCAATCGACACCACTGAAGGTCCGAACCTCGGTCTGCTCCACTCTCTGTCCGTGTACGCACAGGCTAACGAATACGGCTTCCTTGGGCCTCCGTac > 2:137817/1‑250 (MQ=255) gTCTCTGTTTATGGACCAGAACAACCCTCTGT‑CTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGT‑CCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGa < 1:231143/251‑1 (MQ=255) | GGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGT‑CTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGT‑CCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGA > NC_000913/4182556‑4183027 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |