Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,182,576 T→G D444E (GAT→GAG)  rpoB → RNA polymerase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,182,5760TG94.0% 163.3 / ‑0.0 50D444E (GAT→GAG) rpoBRNA polymerase subunit beta
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base G (17/30);  total (19/31)
Fisher's exact test for biased strand distribution p-value = 5.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01

GCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCA‑CAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATT  >  NC_000913/4182328‑4182816
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 
gcACTGGTAGAAATCTACCGCATGATGCGCCCGGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCGGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGAAATCATTGATGTTATGAAAA‑GCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGAGATc                                                                                                                                                                                                                                                <  2:84977/251‑1 (MQ=255)
 cACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGAGATc                                                                                                                                                                                                                                                <  1:337099/251‑1 (MQ=255)
         gAAATCTACCGCATGATGCGCCCTGTCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGGCGATGAGATCGACCACCt                                                                                                                                                                                                                                        <  1:122400/251‑1 (MQ=255)
                aCCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGAGATCGACCACCTCgg                                                                                                                                                                                                                                     <  1:25751/247‑1 (MQ=255)
                       gatgCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGAGATCGACCACCTCGGCAACCGTCGTa                                                                                                                                                                                                                          <  1:308044/251‑1 (MQ=255)
                             cccTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGAGATCGACCACCTCGGCAACCGTCGTAtccgtt                                                                                                                                                                                                                    >  1:188518/1‑251 (MQ=255)
                               cTGGCGAGCCGCCGACTCGTGAAGCAGCTGAACGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGAGATCGACCACCTCGGCAACCGTCGTAtccgttcc                                                                                                                                                                                                                  >  2:371842/1‑251 (MQ=255)
                                           cgACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGAGATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATg                                                                                                                                                                                                      <  1:231712/251‑1 (MQ=255)
                                                             aaaGCCTGTTCGAGATCCTGTTCTTCTCCGAAGACCGGTCTGACTTGTCTGCCGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGATGACATCGCCGGTTCCGGACTCCTGAGCAACGCCGCCATACTTGATGTTATGACAAAGCTCATCGATCTCCGTACCGGTAAAGGCGAGGTCGATGATATCCACCACCTCGGCCACCGTCGTATCCGTTCCGTTGGCGCAATGGCGGAAAACCatgtccgc                                                                                                                                                                                    >  2:28118/1‑244 (MQ=255)
                                                               aGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGAGATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGACATGGCGGAAAACCAGTTCCGCGt                                                                                                                                                                                  >  1:196712/1‑251 (MQ=255)
                                                               aGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGAGATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGt                                                                                                                                                                                  >  2:174154/1‑251 (MQ=255)
                                                                gCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGAGATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGtt                                                                                                                                                                                 <  1:168117/251‑1 (MQ=255)
                                                                  cggTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATAGCCGTAACGGTAAAGGCGAAGTCGATGAGATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTgg                                                                                                                                                                               <  2:196712/249‑1 (MQ=255)
                                                                    gTTCGAGAACCTGTTCTTTTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTAGGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGAGAGCGACCACCTCGGCATCCGGCGTATCCGGTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGc                                                                                                                                                                              <  2:224921/250‑1 (MQ=255)
                                                                    gTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGAGATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTgg                                                                                                                                                                               <  1:205994/249‑1 (MQ=255)
                                                                    gTTCGAGAACCGGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGAGATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGcc                                                                                                                                                                             <  2:7508/251‑1 (MQ=255)
                                                                        gagaACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGAGATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGCAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGt                                                                                                                                                                         >  1:233395/1‑251 (MQ=255)
                                                                                        ccGAAGACCGTTATGACTAGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGCAGAAATCGAAGGGTCCGGTATCCTGAGCGAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCAATGAGATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCg                                                                                                                                                         >  1:96815/1‑251 (MQ=255)
                                                                                                      gACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGAGATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCgtct                                                                                                                                           >  2:297397/1‑251 (MQ=255)
                                                                                                      gACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGAGATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCGGGTACGTGTAGAGCGGGCGGTGAACGAGCgtct                                                                                                                                           >  2:52902/1‑251 (MQ=255)
                                                                                                           gTCTGCGGTTGTTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGGCGATGAGATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGGCTGGTACTTGGAGAGCGTGCGGTGAAAGAGCgtctggct                                                                                                                                       <  1:334719/250‑4 (MQ=255)
                                                                                                            tCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGAGATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTctct                                                                                                                                     >  2:267198/1‑251 (MQ=255)
                                                                                                                 ggTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGAGATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCg                                                                                                                                <  2:98386/251‑1 (MQ=255)
                                                                                                                     ggTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGAGATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATc                                                                                                                             <  1:297397/250‑1 (MQ=255)
                                                                                                                                       cGTTCTCTGCGGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGAGATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCca‑ca                                                                                                         <  1:236280/251‑1 (MQ=255)
                                                                                                                                        gTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGAGATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCca‑c                                                                                                          >  1:347723/1‑249 (MQ=255)
                                                                                                                                            tctGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGAGATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCA‑CAGGat                                                                                                     >  1:40589/1‑250 (MQ=255)
                                                                                                                                               gctgcGCGAAGAAATCTAAGGGTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAATCTCATCGATAGCCGTAACGGTAAAGGCGAAGTCGATGAGAGCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCA‑CAGGata                                                                                                    <  2:224405/248‑1 (MQ=255)
                                                                                                                                                  gcgcGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGAGATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCA‑CAGGATATGAt                                                                                                <  2:80285/249‑1 (MQ=255)
                                                                                                                                                    gcgAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGAGATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCA‑CAGGATATGATCAAc                                                                                            <  1:196192/251‑1 (MQ=255)
                                                                                                                                                      gaagaaATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGAGATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCA‑AAGGATATGATCAACGc                                                                                          >  1:262165/1‑251 (MQ=255)
                                                                                                                                                         gaaATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAATGCGATGAGATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCA‑CAGGATATGATCAACGCCaa                                                                                       <  2:225586/251‑1 (MQ=255)
                                                                                                                                                         gaaATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGAGATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCA‑CAGGATATGATCAACGCCaa                                                                                       <  1:174154/251‑1 (MQ=255)
                                                                                                                                                         gaaATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGAGATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCA‑CAGGATATGATCAACGCCaa                                                                                       <  1:284086/251‑1 (MQ=255)
                                                                                                                                                              cGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGAGATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCAGCGTTGGCCTGGTACGTGTAGAGCGGGCGGTGAAAGAGCGTCTGTCTCTGGGCGGACTGGATACCCTGCTGCCAACAG‑AGATGATCAACGCCAAGCCg                                                                                   >  2:213980/1‑250 (MQ=255)
                                                                                                                                                                        ggTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGAGATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCA‑CAGGATATGATCAACGCCAAGCCGATTTCCgcagc                                                                        <  1:371842/251‑1 (MQ=255)
                                                                                                                                                                                    aaaGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGAGATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCA‑CAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGtt                                                            <  1:175600/251‑1 (MQ=255)
                                                                                                                                                                                    aaaGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGACGATGAGATCGACCACCTCGGCAACCGTCGTATCCGTGCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCA‑CAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGtt                                                            <  1:375241/251‑1 (MQ=255)
                                                                                                                                                                                      aGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCA‑CAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGttct                                                          >  2:302075/1‑251 (MQ=255)
                                                                                                                                                                                      aGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGAGATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCA‑CAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGttct                                                          >  1:283112/1‑251 (MQ=255)
                                                                                                                                                                                         cgacATCATGGATGTTATGAAAAAGCCCATCGATATCCGTAACGGTAAAGGCGAAGTCGAGGAGATCGACCCCCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCA‑CAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTtcgtcg                                                       <  2:233395/251‑1 (MQ=255)
                                                                                                                                                                                          gacATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCA‑CAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCgg                                                      <  1:302075/251‑1 (MQ=255)
                                                                                                                                                                                                atTGATGGTATGAAAAAGCTCATCGATATCCGGAACGGGAAAGGCGAAGTCGATGAGATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTGCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCA‑CAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTccag                                                <  2:55524/251‑1 (MQ=255)
                                                                                                                                                                                                    aTGTTATGAAAAAGCTCATCGATATCGGTAACGGTAAAGGCGCAGTCGATGAGATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCA‑CAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAGAGAGTCATTCGGTTccagcc                                              >  1:297443/1‑249 (MQ=255)
                                                                                                                                                                                                      gTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGAGATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCA‑CAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCcagccagc                                           <  1:191039/250‑1 (MQ=255)
                                                                                                                                                                                                       ttATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGAGATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCA‑CAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTg                                         >  2:11446/1‑251 (MQ=255)
                                                                                                                                                                                                        tATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGAGATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGCTGCCA‑CAGGATATGATCAACGCCAAGCCGATTTCCGCAGCCGTGAACGAGTTCTTCGGTTCCAGCCAGATGt                                        >  2:1278/1‑251 (MQ=255)
                                                                                                                                                                                                                                    ggTAAAGGGGAAGTCGATGGGATCGACCACCTCGGCAACCGTCGTATCCGTTCCGGTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCTTGGTACGGTTAGAGCGGTCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCGGATGCCA‑CAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCTGTTTATGGACCAGAACAACCCGcc            <  2:306835/251‑2 (MQ=255)
                                                                                                                                                                                                                                         aGGCGAAGTCGATGAGATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCA‑CAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCTGTTTATGGACCAGAACAACCCGCTGTCTg       <  1:11446/251‑1 (MQ=255)
                                                                                                                                                                                                                                              aaGTCGATGAGATCGACGACCGCGGCAAGCGTCGTATCCGTGCCGTTGGCGAAATGGCGGAAAACCAGTTCTGCGTTGGACTGGTACGTGTAGAGCGTGCGTTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCA‑CAGGAGATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCTGTTTATGGACCAGAACAACCCGCTGTCTGAGAtt  <  1:262184/251‑1 (MQ=255)
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 
GCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCA‑CAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATT  >  NC_000913/4182328‑4182816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑