| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,154,691 | G→A | Q53* (CAG→TAG) | argE ← | acetylornithine deacetylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,154,691 | 0 | G | A | 92.6% | 74.2 / ‑0.7 | 27 | Q53* (CAG→TAG) | argE | acetylornithine deacetylase |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (9/16); total (10/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.26e-01 | |||||||||||
GGTTTTTTCAGTTTCGTGACGTCGACATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCTCCGTCAGTGTAAACGGATCGCGCGTCCAGCGACCGTCATCAAATGGCACCGTATCGGT‑ATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAACAGGCTGCACTTCCACATTGAAGCCCAAATCTTTAAACCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCAC > NC_000913/4154444‑4154936 | ggTTTTTTCAGTTTCGTGACGTCGACAACGCGTAGCGCATCAAGAATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCTCCGTCAGTGTAAACGGATCGCGCGTCCAGCGACCGTCATCAAATGGCACCGTATCGGT‑ATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAACAGGCTacac < 1:30922/251‑1 (MQ=255) cAGTTTCGTGACGTCGACATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCCCCGTCAGTGTAAACGGATCGCGCGTCCAGCGACCGTCATCAAATGGCACCGTATCGGT‑ATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAACAGGCTACACTTCcaca < 1:142632/250‑1 (MQ=255) cGTCGACATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCTCCGTCAGTGTAAACGGATCGCGCGTCCAGCGACCGTCATCAAATGGCACCGTATCGGT‑ATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAACAGGCTGCACTTCCACATTGAAGCCCaa > 1:191870/1‑250 (MQ=255) cGACATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCTCCGTCAGTGTAAACGGATCGCGCGTCCAGCGACCGTCATCAAATGGCACCGTATCGGT‑ATGCCCCGCCAGCAACAAGCCACCAGCCCCCTGTCCGATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAACAGGCTACACTTCCACATTGAAGCCCAActct > 1:126893/1‑251 (MQ=255) cATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCTCCGTCAGTGTAAACGGATCGCGCGTCCAGCGACCGTCATCAAATGGCACCGTATCGGT‑ATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAACAGGCTACACTTCCACATTGAAGCCCAAATCTTTa < 1:149606/251‑1 (MQ=255) cacAAGGATAACCCAAAAAAAGCTTTGCATGTCCGCGGGGCCTAAGCCGAAAAGCTTGCCGTCATGCTCCGTCAGTGTAAACGATTCGCCCCTCCAGGGCCCGTCATCAAATGGCCCCGTATCGTT‑ATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAACAGGCTACACTTCCACATTGAAGCCCAAATCTTTAAACCAGTCCGCca < 2:199702/249‑1 (MQ=255) aacAAGGATAAACACAAAAAGGCCTTTCAGGTCGGCGGTGCCTAAGCCTTAAAGCTTGCCGTCACGCTCCGCCAGTGTAAACGGAGCGCGCGTCCAGCGACCGTCATCAAATGGCACCGTACCGGT‑ATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATACTTGCCAGCATATAGAATTTGTTGCGAGTTCCTGGAACAGGCTACACTTCCTCATTGAAGCCCAAATCTTTAAACCAGTACGCca < 1:201901/249‑1 (MQ=255) tCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTACCTAAGACGTAAAGCTTGACGTCATGCTCCGTCAGTGTAAACGGATCGCGCGTCCAGCGACCGGCATCAAATGGCACCGTAACGGC‑ATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATACTTGCCAGCATATTGAATTTGGAGGGAGGTCCTGGAACCGGCTAAACTTCCCCATTGAGACCCAACACTTTAAACCAGtacacca > 2:308/1‑244 (MQ=255) aaaaaCGCAAAAAAGCCTTCCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCTCCGTCAGTGTAAACGGATCGCGCGTCCAGCGACCGCCACCAAATGGCCCCGTATCGGT‑ATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAACAGGCTACACTTCcaca < 2:92000/211‑1 (MQ=255) aTAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCTCCGTCAGTGTAAACGGATCGCGCGTCCAGCGACCGTCATCAAATGGCACCGTATCGGT‑ATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAACAGGCTACACTTCcaca > 1:92000/1‑213 (MQ=255) aaaCGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCTCCGTCAGTGTAAACGGATCGCGCGTCCAGCGACCGTCATCAAATGGCACCGTATCGGT‑ATGCCCCGCCAGCCACAAGCCGCCAGACCCCTGTCCGCTACTTGCCAGCATATTGAATTTGTTTCGAGTTCCTGGAACAGGCTACCCTTCCACATTGAAGCCCAAATCTTTAAACCAGTCAGCCAGCagagcgt > 2:102904/1‑248 (MQ=255) gCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCTCCGTCAGTGTAAACGGATCGCGCGTCCAGCGACCGTCATCAAATGGCACCGTATCGGT‑ATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAACAGGCTACACTTCCACATTGAAGCCCAAATCTTTAAACCAGTCCGCCAGCAGAGTGATTa > 2:162015/1‑250 (MQ=255) ttCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCTCCGTCAGTGTAAACGGATCGCGCGTCCAGCGACCGTCATCAAATGGCACCGTATCGGT‑ATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAACAGGCTACACTTCCACATTGAAGCCCAAATCTTTAAACCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCt > 1:176820/1‑251 (MQ=255) gccggcgtTGCCTAAGCCGTAAAGCTTGCCGTCATGCTCCGTCAGTGTAAACGGATCGCGCGTCCAGCGACCTTCATCAAATGGCACCGTATCGGT‑ATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAACAGGCTACACTTCCACATTGAAGCCCAAATCTTTAAACCAGGCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGAt < 1:89595/249‑1 (MQ=255) gtgggtcttaacccttacgtcTTGCCGTCATGCTCCGTCAGTGTAAACGGACCGCGCGCCCAGCGACCGCAATCAACAGGCCCCGGA‑CGGTTATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAACAGGCTACACTTCCACATTGAAGCCCAAATCTTTAAACCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAg < 2:91611/231‑1 (MQ=255) ggTGCCTAAGCCGTAAAGCTTGCCGTCATGCCCCGTCAGTGTAAACGGATCGCGCGTCCAGCGACCGTCATCAAATGGCACCGTATCGGT‑ATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAACAGGCTACACTTCCACATTGAAGCCCAAATCTTTAAACCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTg < 1:262860/251‑1 (MQ=255) catcGACCGTCATCAAATGGCCCCGTATCGGT‑ATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAACAGGCTACACTTCCACATTGAAGCCCAAATCTTTAAACCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATaaa < 1:135967/248‑1 (MQ=255) aaaatggacccgattCGGT‑ATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAACAGGCTACACTTCCACATTGTAGCCCAAATCTTTAAACCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTg < 2:292555/237‑1 (MQ=255) ccGGT‑ATGCCCGGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAACAGGCTACACTTCCACATTGAAGCCCAAATCTTTAAACCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGa < 1:213248/250‑1 (MQ=255) ccGCCAGCAACAAGCCACCAGCCCCCTGTCCGATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAACAGGCTACACTTCCACATTGAAGCCCAAATCTTTAAACCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTg < 2:126893/251‑1 (MQ=255) gCAACAAGCCGCCAGCCCCCTGTCCGATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAACAGGCTGCACTTCCACATTGAAGCCCAAATCTTTAAACCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACCCACCTCTGGTCATg < 2:191870/251‑1 (MQ=255) ccAGCCCCCTGTCCGATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAACAGGCTACACTTCCTCATTGAAGCCCAAATCTTTAAACCAGTCCGCCAGCAGAGTGAGTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTGCATTGTTGACACACCTCTTGTCATGATAGTATCAat < 2:97680/251‑1 (MQ=255) cAGCCCCCCGTCAGATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAACAGGCTACACTTCCACATTGAAGCCCAAATCTTTAAACCAGTCCGCCAGCAGAGTGATTAAATTTGCATTGATTTGATCGAGTACCTCTTCCGTGGCGCTTATTGAAGGTGTGGAAATCAGAGCGCGGTACTTCTCGATAAATGGCGGTAATTTGGTTTTCATTGTTTCAACACCCCTGGGTATGATAGTATCAat > 2:179907/1‑250 (MQ=255) ccccTGTCCGATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAACAGGCTACACTTCCACATTGAAGCCCAAATCTTTAAACCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTc > 1:357468/1‑250 (MQ=255) gATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAACAGGCTACACTTCCACATTGAAGCCCAAATCTTTAAACCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATAAATATTCCTGCAGTAtt > 2:65383/1‑251 (MQ=255) ccGAGTTCCTGGAACATGCTACCCTTCCACATTGAAGCCCAAATCTTTAAACCAGTCCTCCAGCAGAGTTATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGTCGCTTATTTAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGTCGGTAATTTTTTTTTCATTGTTGACACACCTCTGTTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACTTTGac < 2:167578/250‑2 (MQ=255) ggAACAGGCTACACTTCCACATTGAAGCCCAAATCTTTAAACCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGtaataa < 1:162015/249‑1 (MQ=255) aGGCTACACTTCCACATTGAAGCCCAAATCTTTAAACCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTGTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACccac < 2:176820/251‑1 (MQ=255) | GGTTTTTTCAGTTTCGTGACGTCGACATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCTCCGTCAGTGTAAACGGATCGCGCGTCCAGCGACCGTCATCAAATGGCACCGTATCGGT‑ATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAACAGGCTGCACTTCCACATTGAAGCCCAAATCTTTAAACCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCAC > NC_000913/4154444‑4154936 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |