Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,388,041	T→G	T50P (ACG→CCG)	aaeB ←	aromatic carboxylic acid efflux pump subunit AaeB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,388,041	0	T	G	100.0%	104.4 / NA	34	T50P (ACG→CCG)	aaeB	aromatic carboxylic acid efflux pump subunit AaeB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (18/16);  total (18/16)

AATGATCAGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGTACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGCAAA‑GCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATT‑AACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACT  >  NC_000913/3387796‑3388281                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    aaTGATCAGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCAGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACcgg                                                                                                                                                                                                                                                >  2:149370/1‑251 (MQ=255)                   aCCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGACTAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTg                                                                                                                                                                                                                              >  1:196535/1‑251 (MQ=255)                                   gCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCCGTACAGCCCAGCg                                                                                                                                                                                                              >  2:85341/1‑251 (MQ=255)                                     aTACGAGTTTTCTATGCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCAGCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGAGAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTg                                                                                                                                                                                                            <  2:59843/251‑1 (MQ=255)                                     aTACGAGTTTTCTATCCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCAGCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGAGAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCATCAATCGCCGCTGTCAGTACCGCCCAGCGTg                                                                                                                                                                                                            <  2:59837/251‑1 (MQ=255)                                        cgatttttCTATTCGTACCAGCGAGGATATCCCGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCAGCAATAATGGTGCGCGGATCACCGCAAGGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAAGCGCCGCTGTCAGTACCGCCCAGCGTGGCg                                                                                                                                                                                                         <  2:286269/247‑1 (MQ=255)                                          aGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCCGTACCGCCCAGCGTGGCGtt                                                                                                                                                                                                       >  2:291558/1‑251 (MQ=255)                                               tCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCa                                                                                                                                                                                                   <  2:157033/250‑1 (MQ=255)                                                        aCCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGt                                                                                                                                                                                          <  1:98819/250‑1 (MQ=255)                                                                    atCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGGTTCCAGCCGGAAGTGCAAGCcaacaa                                                                                                                                                                              >  2:87107/1‑250 (MQ=255)                                                                                  aCCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACaa                                                                                                                                                               <  1:352247/251‑1 (MQ=255)                                                                                     ggCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGACCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATgg                                                                                                                                                            >  1:126679/1‑251 (MQ=255)                                                                                           gATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCg                                                                                                                                                      >  1:108174/1‑251 (MQ=255)                                                                                                       cAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCg                                                                                                                                         <  1:177350/251‑1 (MQ=255)                                                                                                                              ggATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGCAAA‑GCGAATATGTTGGTTAGCa                                                                                                                 <  1:179864/251‑1 (MQ=255)                                                                                                                              ggATCATCGCAATGATGATCACAAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGAACGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCAAGCTGGAAGAGAAAGCCAACAAACAGCGCCAGAACAATGGCGGACGGC‑AGGTTTaaagcaatggggataagttggtaagg                                                                                                                   >  1:41536/1‑225 (MQ=255)                                                                                                                                         atgaggagcACCAGTCGGGTAAGACAGCCAATAAAGGTGCCGATGATGCGCAAAAAGCCAAGATAGCGAAAAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAAGCGCCGCTGTCAGTCCCGCCCAGCGTGGCGGTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGCAAA‑GCGAATATGTTGGTTAGCAATGGAGaaaac                                                                                                      <  2:6915/243‑2 (MQ=255)                                                                                                                                               atCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑CGGTTTACCGCAAA‑GCGAATATGTTGGTTTGCAATGGAGGAAATAcccct                                                                                                >  1:260167/1‑249 (MQ=255)                                                                                                                                                    cAGTCCGGAAAAACAGCCAAAAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGCAAA‑GCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGa                                                                                           <  2:89952/251‑1 (MQ=255)                                                                                                                                                      gTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑CGTTTTTCCCCAAA‑GAGAGAATATTGGTTAGCAATGgagacaaaaccacacgcgtt                                                                                          >  2:207433/1‑234 (MQ=255)                                                                                                                                                                  aGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAATAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGCAAA‑GCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATT‑AACCAAACTCAc                                                                            <  1:197050/251‑1 (MQ=255)                                                                                                                                                                  aGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTTCCGCAAA‑GCGAATATGTTGGTTAGCAATGGAGAAAATAcgtggggtatcccttatttaattt                                                                             >  1:41024/1‑228 (MQ=255)                                                                                                                                                                  aGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCGCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGCAAA‑GCTAATATGTTGGTTAGCAATGGAGAAAATGCCCAGCGTGATT‑AACCAAACTCAc                                                                            <  2:196535/251‑1 (MQ=255)                                                                                                                                                                     cAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGACCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGCAAAAGGGAAAAT‑TTGGTTAGCAATGGAGAAAATAACCATCGTGATTTAACCaaaacacgc                                                                           >  1:276698/1‑244 (MQ=255)                                                                                                                                                                        tAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGCAAA‑ACGAATATGTTTGTTAGCAATGGGGAAAAAAACCATCGGGATT‑AAACAAACTcccggaagg                                                                      >  1:289733/1‑243 (MQ=255)                                                                                                                                                                           aTGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATCCGGGTCACCGCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGCAAA‑GCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATT‑AACCAAACTCACGCAGGCGAt                                                                   <  2:351280/251‑1 (MQ=255)                                                                                                                                                                            tgtgCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGCAAA‑GCGAATATGTTGGTTAGCAATGGGGAAAATACCCATCGTGATT‑AACCAAACTCACGAAGGCggtg                                                                  >  1:62741/1‑248 (MQ=255)                                                                                                                                                                                              aaaGCCACGATAGCGAATAGCGCCAGAATTCGGTTCACCTCCCGCAGCAAAGTCCTGACCGTCGGCAACAATCGCCGCTGGCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGCAAA‑GCGAATATGTGGGTTAGCAATGGAGAAAATACCCATCGTGATT‑AACCAAACTCACGCAGGCGACGGGCCATTTTACGgaagaa                                                <  2:173878/251‑1 (MQ=255)                                                                                                                                                                                                   cACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGCAAA‑GCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATT‑AACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAAt                                           <  1:291558/251‑1 (MQ=255)                                                                                                                                                                                                     cGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGCAAA‑GCGGAAATGTTGGTTAGCAATGGAGAAAATACCCATCGTAATT‑AACCAAAATCACCCAGGAGGTGGGCCCTTTTAAGGGAGAAAGAAtac                                         >  1:70747/1‑249 (MQ=255)                                                                                                                                                                                                          gCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑CGGTTTTCCGCCAA‑CAGGATATTTTGGTTAGGAATGGGGAAAAAACCCATCGTGATT‑TAACAAACTCACGCCGGCGGTGGGGGATTTTTAGGAagaaagaaaccagaa                                     >  2:327176/1‑243 (MQ=255)                                                                                                                                                                                                          gCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGCAAA‑GCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATT‑AACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATcc                                         <  1:327176/246‑1 (MQ=255)                                                                                                                                                                                                                                           agcaAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGGCGCC‑CGTTTTACCGCCAA‑GCGAATATGTTGGTTAGGAATTGGGAAAATACCCATCGTGCTT‑AAACAAACACACGCAGGCGATGGGCCATTTTTCGGGAGAACGAATCCTGGCTTTCGTCGCGACCTTGTTTGCCCATGaccacccc   >  2:124684/1‑249 (MQ=255)                                                                                                                                                                                                                                            gcaAAGGCCGGACCGGAGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAAAAAACAGCGCCAGTACAATGGCGGTTGCCCGGTTTTACC‑CAAA‑AGGAATATGTTTGGTAGCAATGGAGAAAATAACCCTCGTGATT‑AAACAAACTCCAGCAAGCGAAAGGCCATTTTACGGGAGAACGACACCAGGCTTTCGGCGGGATCTTGTTTTCCAGTggacaacacc  >  2:327115/1‑242 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    AATGATCAGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGTACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGCAAA‑GCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATT‑AACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACT  >  NC_000913/3387796‑3388281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑