Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,329,743	G→T	D261Y (GAT→TAT)	dacB →	peptidoglycan DD‑endopeptidase DacB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,329,743	0	G	T	100.0%	132.4 / NA	38	D261Y (GAT→TAT)	dacB	peptidoglycan DD‑endopeptidase DacB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (16/22);  total (16/22)

AGTGCCCCAAAGCCTGGTGATATGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAAGATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGC‑GCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGT‑CGGACAAC‑ATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGG‑CCGGGTCGGACGCCGTGCGTCAGATCCTGCGCCA  >  NC_000913/3329500‑3329987                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                         agtgaccaacaGCCGGGTGGTATGGCTTTTAGCCGCGTTGTATCTTATTCCCCCGGTACGATGTCCAGCAAGGTTCGCCCCCTCCCCCGGGGTGCTCCCGAAGCGCAATTCTGCGAACTGGATGTGGTCCCAGGCGACCTGATCCGCTGTACGCTGACGGGATGCCTGCCACAACGTTCGGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGTAGGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGtt                                                                                                                                                                                                                                                   <  2:326833/241‑1 (MQ=255)      cccAAAGCCTGGTGATATGGCTTTTATACGAGTGGCATCTTATTACCCCGTTACGCTGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGCTGGAGCCAGCTATGCCGGGGCAATTCTGAAATATGGGTTaaatc                                                                                                                                                                                                                                              >  2:51764/1‑249 (MQ=255)           aGCCTGGGGATATGGCTTTTATACGCGGGGCATCTTATTACACCGTTGAGGTGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGCAGCGCAATACTGCGAACTGGATGAGGTGCCAGGCGACCTGAACCGCTTTACGATGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCGTTTGCCGAGCAGGCTGGGGCCAGCTATGCCGGTGCAATTCTGATATATGAGTTACACCAGGCg                                                                                                                                                                                                                                         >  2:95519/1‑251 (MQ=255)             ccTGGTGATATGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGACGGTGCAATTCTGAAATATGAGTTAAAAAAGGAggg                                                                                                                                                                                                                                       >  2:149973/1‑251 (MQ=255)                            tttatcctcggggcctCTTATTACCCCGTTACGATGTCTAGCCAGGTTCGCACCCTCCCCCGTGGTTCTGCCGAATCGCAATACTGCGAACTGGATGTGGTGCTAGGCGTCCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCg                                                                                                                                                                                                                         <  2:48790/235‑1 (MQ=255)                                          aTCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAAc                                                                                                                                                                                                                                              >  2:368947/1‑215 (MQ=255)                                          aTCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAAc                                                                                                                                                                                                                                              <  1:368947/215‑1 (MQ=255)                                          aTCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGCATCACCTGGTGCGGGACTCTGCCGC‑Gcc                                                                                                                                                                                                         >  1:200127/1‑251 (MQ=255)                                             ttattaCCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTCGAGCGGAACACTGCTGC‑GCCAgc                                                                                                                                                                                                      >  1:13991/1‑250 (MQ=255)                                                        gttcGATGTTCAGCCAGGTACGCACCCTACCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGTTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGTCGTTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCATCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGAGGAACACTGTTGC‑GCCAGACTCAGGTTAAc                                                                                                                                                                                           <  1:318084/248‑1 (MQ=255)                                                             aggTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGC‑GCCAGACTCAGGTTAACGAAcc                                                                                                                                                                                      <  1:234228/249‑1 (MQ=255)                                                             aggTTCACCCAGGTCCGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATTCGGCGAACGGGATTGGGTGCCAGGGGACCTGACCCGCTTTACGCTGCCGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGC‑GCCAGACTCAGGTTAACGAAcc                                                                                                                                                                                      <  2:223132/249‑1 (MQ=255)                                                                            cGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGACCTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTTGGGCCGAACAACGCTGCCGCC‑GACTCAGGTTTACGGACCCGGAAAGGTAGGTGc                                                                                                                                                                       >  2:273549/1‑251 (MQ=255)                                                                               ccccgcccccGGGGTTCTGCAGAGGCGCAATACTGCGAACTGGATGGGGTGCAAGGGGGCCTGAACCGCTTTACGCTGCCGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGC‑GCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAg                                                                                                                                                                    <  2:149617/246‑1 (MQ=255)                                                                                     ccccGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCTCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGC‑GCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACa                                                                                                                                                              <  1:123264/251‑1 (MQ=255)                                                                                                 gCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGC‑GCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCt                                                                                                                                                  <  1:63414/251‑1 (MQ=255)                                                                                                              ccTGCGAACTGGAGGTGGTGCAAGGCGACGTGAACCTCTTTACGCTGATGGGGTGCCTGCCACAACTTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCTAGCTATGCCGGTGCAATGCTGAAATATGAGTTAAAACAGGCGGGTAGCACCTGGAGCGGAACACTGCTGC‑GCCAGTCTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCtt                                                                                                                                     <  2:360758/250‑1 (MQ=255)                                                                                                                                            tGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGC‑GCCAGACTCAGGTTAACGAACCTGGAACGGGAGTTGCCAGGAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAAT‑CGGACAAC‑ATg                                                                                                     >  1:109847/1‑251 (MQ=255)                                                                                                                                             gAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGC‑GCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGT‑CGGACAAC‑At                                                                                                      <  1:271947/249‑1 (MQ=255)                                                                                                                                                           tGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGGACACTGCTGC‑GCCAGACTCAGGTTTACGAACCTgg                                                                                                                                                                                   >  2:154223/1‑160 (MQ=255)                                                                                                                                                           tGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGC‑GCCAGACTCAGGTTAACGAACCTgg                                                                                                                                                                                   <  1:154223/160‑1 (MQ=255)                                                                                                                                                            gACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGGATCACCTGGAGCGGAACACTGCTGC‑GCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACCGTCGGCCCCGCTGCCCGAACTGCTTAAGATTATGTTGGAAAAAT‑CGGGAAAC‑ATGATCGCCCATACgggtt                                                                                     >  2:63057/1‑248 (MQ=255)                                                                                                                                                              cGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGC‑GCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGT‑CGGACAAC‑ATGATCGCCGATACGGTTTTc                                                                                   <  1:293912/251‑1 (MQ=255)                                                                                                                                                                    gccttccACAACGTTCTGAGCCGCTCCCGTTGTCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGC‑GCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGT‑CGGACAAC‑ATGATCGCCGATACGGTTTTCCGCAt                                                                              <  2:15355/250‑1 (MQ=255)                                                                                                                                                                    ccctgccGCAACGGTCTGGGCCGCTCCCGTTGGCTTTTGCCGGGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGC‑GCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTAGGCTGAAAAAGT‑CGGACAAC‑ATGATCGCCGATACGGTTTTCCGCATg                                                                             <  2:41874/250‑1 (MQ=255)                                                                                                                                                                        gccacatggTTCTGATCCGCTCACGTTGGCTTTTGCCGGGCAGGATGGAGCCAGCGAGGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACGCTGCTGC‑GCCAGACGCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGT‑CGGACAAC‑ATGATCGCCGATACGGTTTTCCGCATGAt                                                                           <  1:206300/241‑1 (MQ=255)                                                                                                                                                                          cacaACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGC‑GCCAGACTCCGGCTAACGAACCTGGAACCGGAGTTGCCCATAAACAGTCGGCCCCCCCGCCCCATCTGCTTAAGATTATGCTGCAAACGTACGG‑CAACCATTA‑CGCCGATAAGGTTTTCCGCCTGATAggg                                                                       >  2:80868/1‑250 (MQ=255)                                                                                                                                                                                     gAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGC‑GCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGT‑CGGACAAC‑ATGATCGCCg                                                                                              <  2:241493/217‑1 (MQ=255)                                                                                                                                                                                     gAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGC‑GCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGT‑CGGACAAC‑ATGATCGCCg                                                                                              >  1:241493/1‑217 (MQ=255)                                                                                                                                                                                                       tgtGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGC‑GCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGTCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGT‑CGGACAAC‑ATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACAt                                           <  1:171701/248‑1 (MQ=255)                                                                                                                                                                                                              tGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGC‑GCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGATGAAAAAGT‑CGGACAAC‑ATGATCGCCGATACGGTTTTTCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACAAGGCGGG‑cc                                  >  1:302123/1‑251 (MQ=255)                                                                                                                                                                                                                               tATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGGACACTGCTGC‑GCCAGACTCAGGTTAACGAACCTGGGAAGGGAGTTGCCAGTAAACAGTCGGCCCCGCTGCCCGATCTTCTTAAGATTATGCTGAAAAAGA‑CGGACAAC‑ATGATCCCCCATACGGTTTTCCGCATAGTAGGCCATGGGCGCCTCCATGTGCCTGGGACCTTGCGGg‑gcggggcgggcggcgggcg                 >  2:40352/1‑241 (MQ=255)                                                                                                                                                                                                                                  gCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGC‑GCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGT‑CGGACAAC‑ATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGG‑CCGGGTCGGACGCCGTGCGTc               <  1:51764/250‑1 (MQ=255)                                                                                                                                                                                                                                  gCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGC‑GCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGT‑CGGACAAC‑ATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGG‑CCGGGTCGGACGCCGTGCGTc               <  1:95519/250‑1 (MQ=255)                                                                                                                                                                                                                                     ggTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGGGAGGAACACTGCTGC‑GCCAGAATCAGGGTAACGAACCTGGGACGGTAGTTGCCCGGAAACAGTCGGGCCCGCAGCACGCTCTGCTTAAGATTATGCTGGAAAAGT‑CGGCCAAC‑ATGATCGCCGATACGGTTTTACGCATGATAAGCCATGCGCGCTTCAATGTGCCTGGAACCTGGCGGGGCCGGGtggtcgccccgcgggcgat            >  2:75990/1‑233 (MQ=255)                                                                                                                                                                                                                                        gCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGC‑GCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGT‑CGGACAAC‑ATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGG‑CCGGGTCGGACGCCGTGCGTCAGATcc         <  1:107565/250‑1 (MQ=255)                                                                                                                                                                                                                                         cAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGC‑GCCAGACTCAGGTTAACGAACCTGGAACGGGAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAAT‑CGGACAAC‑ATGATCGCCGATACGGTTTTCCGCATGGTAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGG‑CCGGGTCGGACGCCCTGCGTCAGATcccc       >  1:8045/1‑249 (MQ=255)                                                                                                                                                                                                                                                gAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGC‑GCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGT‑CGGACAAC‑ATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGG‑CCGGGTCGGACGCCGTGCGTCAGATCCTGCGCCa  <  2:306521/249‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                         AGTGCCCCAAAGCCTGGTGATATGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAAGATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGC‑GCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGT‑CGGACAAC‑ATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGG‑CCGGGTCGGACGCCGTGCGTCAGATCCTGCGCCA  >  NC_000913/3329500‑3329987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑