Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,686,156	G→A	E108K (GAA→AAA)	hmp →	nitric oxide dioxygenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,686,156	0	G	A	96.8%	100.3 / NA	31	E108K (GAA→AAA)	hmp	nitric oxide dioxygenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (18/12);  minor base C (1/0);  total (19/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

CCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAACGCTATTGCCGCCTACGCCAGTAATATTGAAAACCTGCCTGCGCTGCTGCCAGCGGTAGAAAAAATCGCGCAGAAGCACACCAGCTTCCAGATCAAACCGGAACAGTACAACATCGTCGGTGAACACCTGTTGGCAACGCTGGACGAAATGTTCAGCCCGGGGCAGGAAGTGCTGGACGCGTGGGGTAAAGCCTATGGTGTA‑CTGGCTAATGTATTTATCAATCGCGAGGCGGAAATCTATAACGAAAACGCCAGCAAAGCCGGTGGTTGGGAAGGTACTCGCGATTTCCGCATTGTGGCTAAAACACCGCGCAGCGCGCTTATCACCAGCTTCGAACTGGAGCCGGTCGACGGTGGCGCAGTGGCAGAATACCGTCCGGGGCAATATCT  >  NC_000913/2685912‑2686400                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      ccATTTCTACGACCTGAGTTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCTTAATGGCGATCAACGTGAAGCCCTTTATAACGCTATTGCCGCCTACGCCAGTAATATTGAAAACCTGCCTGCGCTGCTGCCAGCGGTAGAAAAAATCGCGCAGAAGCACACCAGCTTCCAGATCAAACCGGAACAGTACAACATCGTCGGTGAACACCTGTTGGCAACGCTGGACAAAATGt                                                                                                                                                                                                                                                 <  2:84406/251‑1 (MQ=255)      tCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAACGCTATTGCCGCCTACGCCAGTAATATTGAAAACCTGCCTGCGCTGCTGCCAGCGGTAGAAAAAATCGCGCAGAAGCACACCAGCTTCCAGATCAAACCGGAACAGTACAACATCGTCGGTGAACACCTGTTGGCAACGCTGGACAAAATGTTCAGc                                                                                                                                                                                                                                            >  1:231812/1‑251 (MQ=255)           gACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAACGCTATTGCCGCCTACGCCAGTAATATTGAAAACCTGCCTGCGCTGCTGCCAGCGGTAGAAAAAATCGCGCAGAAGCACACCAGCTTCCAGATCAAACCGGAACAGTACAACATCGTCGGTGAACACCTGTTGGCAACGCTGGACAAAATGTTCAGCCCggg                                                                                                                                                                                                                                       >  1:288722/1‑251 (MQ=255)                           aTAACCCAGAACTCAAAGAAAGTTGTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAAAGCTATTGCCGCCTACGCCAGTAATATTGAAAACCTGCCTGCGCTGCTGCCAGCGGTAGAAAAAATCGCGCAGAAGCACACCAGCTTCCAGATCAAACCGGAACAGTACAACATCGTCGGTGAACACCTGTTGGCAACGCTGGACAAAATGTTCAGCCCGGGGCAGGAAGTGCTGGAc                                                                                                                                                                                                                       <  1:96346/251‑1 (MQ=255)                               cccAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAACGCTATTGCCGCCTACGCCAGTAATATTGAAAACCTGCCTGCGCTGCTGCCAGCGGTAGAAAAAATCGCGCAGAAGCACACCAGCTTCCAGATCAAACCGGAACAGTACAACATCGTCGGTGAACACCTGTTGGCAACGCTGGACAAAATGTTCAGCCCGGGGCAGGAAGTGCTGGACGCGt                                                                                                                                                                                                                   >  1:322880/1‑251 (MQ=255)                                                     aaCATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAACGCTATTGCCGCCTACGCCAGTAATATTGAAAACCTGCCTGCGCTGCTGCCAGCGGTAGAAAAAATCGCGCAGAAGCACACCAGCTTCCAGATCAAACCGGAACAGTACAACATCGTCGGTGAACACCTGTTGGCAACGCTGGACAAAATGTTCAGCCCGGGGCAGGAAGTGCTGGACGCGTGGGGTAAAGCCTATGGTGTA‑c                                                                                                                                                                                             <  1:220527/250‑1 (MQ=255)                                                       cATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAACGCTATTGCCGCCTACGCCAGTAATATTGAAAACCTGCCTGCGCTGCTGCCAGCGGTAGAAAAAATCGCGCAGAAGCACACCAGCTTCCAGATCAAACCGGAACAGTACAACATCGTCGGTGAACACCTGTTGGCAACGCTGGACAAAATGTTCAGCCCGGGGCAGGAAGTGCTGGACGCGTGGGGTAAAGCCTATGGTGTA‑CTgg                                                                                                                                                                                          >  1:331715/1‑251 (MQ=255)                                                       cATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAACGCTATTGCCGCCTACGCCAGTAATATTGAAAACCTGCCTGCGCTGCTGCCAGCGGTAGAAAAAATCGCGCAGAAGCACACCAGCTTCCAGATCAAACCGGAACAGTACAACATCGTCGGTGAACACCTGTTGGCAACGCTGGACAAAATGTTCAGCCCGGGGCAGGAAGTGCTGGACGCGTGGGGAAAAGCCTATGGTGTA‑CTgg                                                                                                                                                                                          <  2:36395/251‑1 (MQ=255)                                                                          ggCGCTCACCGTGAAGCCCTGTTTAACGCTTTTGCCGCCTACGCCAGAAATATTGAAAACCTGCCTGCGCTGCTGCCAGCGGGAGAAAAAATCGCGCAGAAGCACACCAGCTTCCCGATCAAACCGGAACAGTACAACATCGTCGGTGAACACCTGTTGGCAACGCTGGACAAAATGTTCAGCCAGGGGCAGGACGTACTGGACGCAGGGGGTAAAGCCTATGG‑GTATCTGGCACATGGTTTTATCAATcg                                                                                                                                                                       >  2:168662/1‑251 (MQ=255)                                                                                              gTTTAACGCTATTGCCGCCTACGCCAGTAATATTGAAAACCTGCCTGCGCTGCTGCCAGCGGTAGAAAAAATCGCGCAGAAGCACACCAGCTTCCAGATCAAACCGGAACAGTACAACATCGTCGGTGAACACCTGTTGGCAACGCTGGACAAAATGTTCAGCCCGGGGCAGGAAGTGCTGGACGCGTGGGGTAAAGCCTATGGTGTA‑CTGGCTAATGTATTTATCAATCGCGAGGCGGAAATCTATAACg                                                                                                                                                   <  2:7554/251‑1 (MQ=255)                                                                                                                  aCGCCAGTAATATTGAAAACCTGCCTGCGCTGCTGCCAGCGGTAGAAAAAATCGCGCAGAAGCACACCAGCTTCCAGATCAAACCGGAACAGTACAACATCGTCGGTGAACACCTGTTGGCAACGCTGGACAAAATGTTCAGCCCGGGGCAGGAAGTGCTGGACGCGTGGGGTAAAGCCTATGGTGTA‑CTGGTTAATGTATTTATCAATCGCGAGGCGGAAATCTATAACGAAAACGCCAGCAAAGCCgg                                                                                                                                <  1:168709/250‑1 (MQ=255)                                                                                                                      cAGTAATATTGAAATCCTGCCGTCGCTGCGGCCAGCAGTAGAAAAAATCGCGCAGAAGCACACCAGCTTCCAGATCAAACCGGAACAGGACAACATCGTCGGTGAACTCCTGTTGGCAACGCTGGACAAAATGTGCAGCCCGGGGCAGGAAGTGCTGGTCGCGTGGGGTAAAGCCTATGGTGTA‑CTGGCTTATGTATTTATCAATCGCTAGTCGGAAATCTATACCGAAAACGTCAGCAAAGCCGGTGGtt                                                                                                                           <  2:224890/251‑1 (MQ=255)                                                                                                                                                      cAGCGGTAGAAAAAATCGCGCAGAAGCACACCAGCTTCCAGATCAAACCGGAACAGTACAACATCGTCGGTGAACACCTGTTGGCAACGCTGGACAAAATGTTCAGCCCGGGGCAGGAAGTGCTGGACGCGTGGGGTAAAGCCTATGGTGTA‑CTGGCTAATGTATTTATCAATCGCGAGGCGGAAATCTATAACGAAAACGCCAGCAAAGCCGGTGGTTGGGAAGGTACTCGCGATTTCCGCATTGTGGCt                                                                                           >  1:63320/1‑251 (MQ=255)                                                                                                                                                       aGCGGTAGAAAAAATCGCGCAGAAGCACACCAGCTTCCAGATCAAACCGGAACAGTACAACATCGTCGGTGAACACCTGTTGGCAACGCTGGACAAAATGTTCAGCCCGGGGCAGGAAGTGCTGGACGCGTGGGGTAAAGCCTATGGTGTA‑CTGGCTAATGTATTTATCAATCGCGAGGCGGAAATCTATAACGAAAACGCCAGCAAAGCCGGTGGTTGGGAAGGTACTCGCGATTTCCGAAtttgggct                                                                                           >  2:296199/1‑244 (MQ=255)                                                                                                                                                             aGAAAAAATCGCGCAGAAGCACACCAGCTTCCAGATCAAACCGGAACAGTACAACATCGTCGGTGAACACCGGTTGGCAACGCTGGACAAAATGTTCAGCCCGGGGCAGGAAGTGCTGGACGCGTGGGGTAAAGCCTATGGTGTA‑CTGGCTAATGTATTTATCAATCGCGAGGCGGAAATCTATAACGAAAACGCCAGCAAAGCCGGTGGTTGGGAAGGTACTCGCGATTTCCGCATTGTGGCTAAAacac                                                                                    <  2:331715/251‑1 (MQ=255)                                                                                                                                                                      cgcgCAGAAGCACACCAGCTTCCAGATCAAACCGGAACAGTACAACATCGTCGGTGAACACCTGTTGGCAACGCTGGACAAAATGTTCAGCCCGGGGCAGGAAGTGCTGGACGCGTGGGGTAAAGCCTATGGTGTA‑CTGGCTAATGTATTTATCAATCGCGAGGCGGAAATCTATAACGAAAACGCCAGCAAAGCCGGTGGTTGGGAAGGTACTCGCGATTTCCGCATTGTGACTAAAACACCGCGCAgc                                                                            >  1:95074/1‑250 (MQ=255)                                                                                                                                                                                   acCAGCTTCCAGATCAAACCGGAACAGTACAACATCGTCGGTGAACACCTGTTGGCAACGCTGGACAAAATGTTCAGCCCGGGGCAGGAAGTGCTGGACGCGTGGGGTAAAGCCTATGGTGTA‑CTGGCTAATGTATTTATCAATCGCGAGGCGGAAATCTATAACGAAAACGCCAGCAAAGCCGGTGGTTGGGAAGGTACTCGCGATTTCCGCATTGTGGCTAAAACACCGCGCAGCGCGCTTATCAcc                                                                >  1:102901/1‑249 (MQ=255)                                                                                                                                                                                          tCCAGAACAAACCGGAACAGTACAACATCGGCGGTGAACACCTGTTGGCAACGCTGGACAAAATGTTCAGCCCGGGGCAGGAAGTGCTGGACGCGTGGGGTAAAGCCTATGGTGTA‑CTGGCTAATGTATTTATCAATCGCGAGGCGGAAATCTATAACGAAAACGCCAGCAAAGCCGGTGGTTGGGAAGGTACTCGCGATTTCCGCATTGTGGCTAAAACACCGCGCAGCGCGCTTATCACCAGCTTCg                                                         <  2:66748/249‑1 (MQ=255)                                                                                                                                                                                                tCAAACCGGAACAGTACAACATCGTCGGTGAACACCTGTTGGCAACGCTGGACAAAATGTTCAGCCCGGGGCAGGAAGTGCTGGACGCGTGGGGTAAAGCCTATGGTGTA‑CTGGCTAATGTATTTATCAATCGCGAGGCGGAAATCTATAACGAAAACGCCAGCAAAGCCGGTGGTTGGGAAGGTACTCGCGATTTCCGCATTGTGGCTAAAACACCGCGCAgcgcg                                                                         >  1:218911/1‑227 (MQ=255)                                                                                                                                                                                                tCAAACCGGAACAGTACAACATCGTCGGTGAACACCTGTTGGCAACGCTGGACAAAATGTTCAGCCCGGGGCAGGAAGTGCTGGACGCGTGGGGTAAAGCCTATGGTGTA‑CTGGCTAATGTATTTATCAATCGCGAGGCGGAAATCTATAACGAAAACGCCAGCAAAGCCGGTGGTTGGGAAGGTACTCGCGATTTCCGCATTGTGGCTAAAACACCGCGCAgcgcg                                                                         <  2:218911/227‑1 (MQ=255)                                                                                                                                                                                                     ccGGAACAGTACAACATCGTCGGTGAACACCTGTTGGCAACGCTGGACAAAATGTTCAGCCCGGGGCAGGAAGTGCTGGACGCGTGGGGTAAAGCCTATGGTGTA‑CTGGCTAATGTATTTATCAATCGCGAGGCGGAAATCTATAACGAAAACGCCAGCAAAGCCGGTGGTTGGGAAGGTACTCGCGATTTCCGCATTGTGGCTAAAACACCGCGCAGCGCGCTTATCACCAGCTTCGAACTGGAGCCGGt                                            >  1:88880/1‑251 (MQ=255)                                                                                                                                                                                                       ggAACAGTACAACATCGTCGGTGAACACCTGTTGGCAACGCTGGACAAAATGTTCAGCCCGGGGCAGGAAGTGCTGGACGCGTGGGGTAAAGCCTATGGTGTA‑CTGGCTAATGTATTTATCAATCGCGAGGCGGAAATCTATAACGAAAACGCCAGCAAAGCCGGTGGTTGGGAAGGGACTCGCGATTTCCGCATTGTGGCTAAAACACCGCGCAGCGCGCTTATCACCAGCTTCGAGCTGGAGCCGGTCg                                          >  1:141217/1‑251 (MQ=255)                                                                                                                                                                                                          aCAGAACAACATCGTCGGTAAACACCTGTTGGCAACGCTGGACAAAATGTTCAGCCCGGGGCAGGAAGTGCTGACCGCGTGGGGTAAAGCCTATGGTGTA‑CTGGCTAATGTATTTATCACTCGCGAGGCGGAAATCTATAACGAAAACGCCAGCAAAGCAGGTGGTTGGGAAGGTACTCGCGATTTACGCATTGTGGCTAAAACCACGCGCCGCGCGCTTATACCCAGCTTCGAACGGGAGCCGGTCGACg                                       >  2:242949/1‑251 (MQ=255)                                                                                                                                                                                                                caacaTCGTCGGTGAACACCTGTTGGCAACGCTGGACCAAATGTTCAGCCCGGGGCAGGAAGTGCTGGACGCGTGGGGTAAAGCCTATGGTGTA‑CTGGCTAATGTATTTATCAATCGCGAGGCGGAAATCTATAACGAAAACGCCAGCAAAGCCGGTGGTTGGGCAGGTACTCGCGATTTCCGCATTGTGGCTAAAACACCGCGCAGCGCGCTTATCCCCAGCTTCGCACTGGAGCCGGTCGACGGGGgcg                                 >  2:181420/1‑251 (MQ=255)                                                                                                                                                                                                                 aacaTCGTCGGTGAACACCTGTTGGCAACGCTGGACAAAATGTTCAGCCCGGGGCAGGAAGTGCTGGACGCGTGGGGTAAAGCCTATGGTGTA‑CTGGCTAATGTATTTATCAATCGCGAGGCGGAAATCTATAACGAAAACGCCAGCAAAGCCGGTGGTTGGGAAGGTACTCGCGATTTCCGCATTGTGGCTAAAACACCGCGCAGCGCGCTTATCCCCAGCTTCGAACTGGAGCCGGTCGACGGTGgcgc                                >  2:184199/1‑251 (MQ=255)                                                                                                                                                                                                                    aTCGTCGGTGAACACCTGTTGGCAACGCTGGACAAAATGTTCAGCCCGGGGCAGGAAGTGCTGGACGCGTGGGGTAAAGCCTATGGTGTA‑CTGGCTAATGTATTTATCAATCGCGAGGCGGAAATCTATAACGAAAACGCCAGCAAAGCCGGTGGTTGGGAAGGTACTCGCGATTTCCGCATTGTGGCTAAAACACCGCGCAGCGCGCTTATCACCAGCTTCGAACTGGAGCCGGTCGACGGTGGCGCAGt                             >  1:106469/1‑251 (MQ=255)                                                                                                                                                                                                                             gAACACCTGTTGGCAACGCTGGACAAAATGTTCAGCCCGGGGCAGGAAGTGCTGGACGCGTGGGGTAAAGCCTATGGTGTA‑CTGGCTAATGTATTTATCAATCGCGAGGCGGAAATCTATAACGAAAACGCCAGCAAAGCCGGTGGTTGGGAAGGTACTCGCGATTTCCGCATTGTGGCTAAAACACCGCGCAGCGCGCTTATCACCAGCTTCGAACTGGAGCCGGTCGACGGTGGCGCAGTGGCAGAAt                     >  1:91144/1‑250 (MQ=255)                                                                                                                                                                                                                              aaCACCTGTTGGCAACGCTGGACAAAATGTTCAGCCAGGGGCAGGAAGTGCTGGACGCGTGGGGTAAAGCCTATGGTGTA‑CTGGCTAATGTATTTATCAATCGCGAGGCGGAAATCTATAACGAAAACGCCAGCAAAGCCGGTGGTTGGGAAGGTACTCGCGATTTCCGCATTGTGGCTAAAACACCGCGCAGCGCGCTTATCACCAGCTTCGAACTGGAGCCGGTCGACGGTGGCGCAGTGGCAGAATAc                   <  1:28681/251‑1 (MQ=255)                                                                                                                                                                                                                                 aacggTTGGCAACGCTGGACAAAATGTTCAGCCCGGGGCAGGAAGTGCTGGACGCGTGGGGTAAAGCCTATGGTGTA‑CTGGCTAATGTATTTATCAATCGCGAGGCGGAAATCTATAACGAAAACGCCAGCATAGCCGGTGGTTGGGAAGGTACTCGCGATTGCCGCATTGTGGCTAAAACACCGCGCAGCGCGCTTATCACCAGCTTCGAACTGGAGCCGGTCGACGGTGGCGCAGTGGCAGAATACCGt                <  1:121838/247‑1 (MQ=255)                                                                                                                                                                                                                                            aCGCTGGACAAAATGTTCAGCCCGGGGCAGGAAGTGCTGGACGCGTGGGGTAAAGCCTATGGTGTA‑CTGGCTAATGTATTTATCAATCGCGAGGCGGAAATCTATAACGAAAACGCCAGCAAAGCCGGTGGTTGGGAAGGTACTCGCGATTTCCGCATTGTGGCTAAAACACCGCGCAGCGCGCTTATCACCAGCTTCGAACTGGAGCCGGTCGACGGTGGCGCAGTGGCAGAATACCGTCCGGGGCAat      >  1:74568/1‑250 (MQ=255)                                                                                                                                                                                                                                               cTGGACAAAATGTTCAGCCCGGGGCAGGAAGTGCTGGACGCGTGGGGTAAAGCCTATGGTGTA‑CTGGCTAATGTATTTATCAATCGCGAGGCGGAAATCTATAACGAAAACGCCAGCAAAGCCGGTGGTTGGGAAGGTACTCGCGATTTCCGCATTGTGGCTAAAACACCGCGCAGCGCGCTTATCACCAGCTTCGAACTGGAGCCGGTCGACGGTGGCGCAGTGGCAGAATACCGTCCGGGGCAATAtct  >  1:183291/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      CCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAACGCTATTGCCGCCTACGCCAGTAATATTGAAAACCTGCCTGCGCTGCTGCCAGCGGTAGAAAAAATCGCGCAGAAGCACACCAGCTTCCAGATCAAACCGGAACAGTACAACATCGTCGGTGAACACCTGTTGGCAACGCTGGACGAAATGTTCAGCCCGGGGCAGGAAGTGCTGGACGCGTGGGGTAAAGCCTATGGTGTA‑CTGGCTAATGTATTTATCAATCGCGAGGCGGAAATCTATAACGAAAACGCCAGCAAAGCCGGTGGTTGGGAAGGTACTCGCGATTTCCGCATTGTGGCTAAAACACCGCGCAGCGCGCTTATCACCAGCTTCGAACTGGAGCCGGTCGACGGTGGCGCAGTGGCAGAATACCGTCCGGGGCAATATCT  >  NC_000913/2685912‑2686400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑