Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,339,173	G→A	S83L (TCG→TTG)	gyrA ←	DNA gyrase subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,339,173	0	G	A	97.7%	129.4 / ‑4.0	44	S83L (TCG→TTG)	gyrA	DNA gyrase subunit A
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (24/19);  total (24/20)
Fisher's exact test for biased strand distribution p-value = 4.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.89e-01

CAGGTTAGGAATTTTGGTTGGCATGACGTCCGGAATTTTTTCCGTGCCGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCGGTGTAATTTCTCTCGCAAGGTCG  >  NC_000913/2338923‑2339415                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                   cagGTTAGGAATTTTGGTTGGCATGACGTCCGGAATTTTTTCCGTGCCGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGAccgccg                                                                                                                                                                                                                                                    >  1:99910/1‑251 (MQ=255)                   tggCATGACGTCCGGAATTTTTTCCGTGCCGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCAAGTCACCATGGGGATgg                                                                                                                                                                                                                                   <  2:118019/250‑1 (MQ=255)                   tggCATGACGCCCGGAATTTTTTCCGTGCCGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCAAGTCACCATGGGGATgg                                                                                                                                                                                                                                   <  2:368687/250‑1 (MQ=255)                            gTCCGGAATTTTTTCCGTGCCGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCAAGTCACCATGGGGATGGTATTTACCg                                                                                                                                                                                                                          >  1:235953/1‑250 (MQ=255)                                               ccGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCAAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACg                                                                                                                                                                                                      >  2:158826/1‑251 (MQ=255)                                                 gTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCAAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACggg                                                                                                                                                                                                    <  1:180445/251‑1 (MQ=255)                                                    aTAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGCCCGCCAAGTCACCATGGGGCTGGTATTTACCGCTTACGt                                                                                                                                                                                                                   >  2:327915/1‑233 (MQ=255)                                                    aTAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCAAGTCACCATGGGGATGGTATTTACCGATTACGt                                                                                                                                                                                                                   <  1:327915/233‑1 (MQ=255)                                                              aCGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCAAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTtata                                                                                                                                                                                       <  1:158826/251‑1 (MQ=255)                                                              aCGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGGCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCAAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTtata                                                                                                                                                                                       <  1:248290/251‑1 (MQ=255)                                                                  aaTCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGCTTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCAAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAAAGACAAGGGCAGATTTTTTATAGGCt                                                                                                                                                                                   >  2:52288/1‑251 (MQ=255)                                                                                ttttCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCAAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTc                                                                                                                                                                        <  1:17285/248‑1 (MQ=255)                                                                                         tCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTACCGCCGCAGAGTCGCCGTCGATAGAACAGAAGTTACCCTGACCGTCTACCAGCATCTAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGAACGCCAAGTAACCATGGGGATGGAATTTACCGATTACGTCACCACCGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTAc                                                                                                                                                            >  1:341994/1‑251 (MQ=255)                                                                                                  aGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCAAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGc                                                                                                                                                   >  2:294370/1‑251 (MQ=255)                                                                                                                          cGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCAAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCa                                                                                                                                                                           >  1:20625/1‑203 (MQ=255)                                                                                                                          cGGATTTCCGTATAACGCATTGCCGCCGCAGAGGCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCAAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCa                                                                                                                                                                           <  2:20625/203‑1 (MQ=255)                                                                                                                            gATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGCCCGCCAAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCt                                                                                                                         >  2:17868/1‑251 (MQ=255)                                                                                                                            gATTTCCGGATAACGCATTGCCGCCGCAGCGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCAAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACAAGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATTGAGTTAAGTACGCGGCAGGGTACCGGCt                                                                                                                         >  2:304972/1‑251 (MQ=255)                                                                                                                                            aTTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCCTATCACGCAGCGAGAATGGCTGCGGCATGCGGACGATCGTGTCATAGACCGCCAAGTCACCATGGGGATGGTATTTAACGATTACGTCACAAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGACTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGa                                                                                                          >  2:295490/1‑250 (MQ=255)                                                                                                                                                    cgcAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCAAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCAACTCGGACATCTGGc                                                                                                  >  2:154993/1‑250 (MQ=255)                                                                                                                                                      cAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCAAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCCTGGCGTAAAGTACGCGACGgtgga                                                                                                                               >  2:126616/1‑217 (MQ=255)                                                                                                                                                      cAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCAAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTa                                                                                                                               <  1:126616/219‑1 (MQ=255)                                                                                                                                                                gTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCAAGTCACCATGGGGATGGTa                                                                                                                                                                                                                                 <  1:323264/111‑1 (MQ=255)                                                                                                                                                                gTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCAAGTCACCATGGGGATGGTa                                                                                                                                                                                                                                 >  2:323264/1‑111 (MQ=255)                                                                                                                                                                   gATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCAAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCcaacaa                                                                                  <  1:52288/251‑1 (MQ=255)                                                                                                                                                                    aTAGAACCGAAGTTACCCTGGCCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAAt                                                                                 <  2:52096/251‑1 (MQ=255)                                                                                                                                                                       gAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCAAGTCACCATGGGGATGGTATTTACCGATTACGTCACCACCGAAACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGAGTAAAGTACGCGACGGGGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGAACGGCCAACAATGAc                                                                              >  2:76972/1‑251 (MQ=255)                                                                                                                                                                                  aCCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCAAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCa                                                                    <  2:316851/250‑1 (MQ=255)                                                                                                                                                                                    ccggACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCAGGCGGACGATCGGGTCATAGACCGCCAAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATaa                                                                 <  2:224652/248‑1 (MQ=255)                                                                                                                                                                                    ccTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCAAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATa                                                                  >  1:322753/1‑250 (MQ=255)                                                                                                                                                                                           gTCTACCAGCATATAACGCAGCGAGACAGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCAAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATcc                                                              >  1:327033/1‑247 (MQ=255)                                                                                                                                                                                                      tataACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCAAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGAcc                                                                             >  2:1979/1‑221 (MQ=255)                                                                                                                                                                                                      tataACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCAAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGAcc                                                                             <  1:1979/221‑1 (MQ=255)                                                                                                                                                                                                      gataACGCAGCGCAAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCAAGTCACCATGGGGATGGTATTTACAGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTACTGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGCCCTTTGGCAGCGCACGGCCAACAATGAACGACATCGCATAATCCAGATAGGagctcttc                                               >  2:305951/2‑251 (MQ=255)                                                                                                                                                                                                       ataACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCAAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGagctcttc                                               >  2:65517/1‑250 (MQ=255)                                                                                                                                                                                                              aGCGAGATTGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCAAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGagctcttcagctcttc                                       <  2:295050/251‑1 (MQ=255)                                                                                                                                                                                                                 gagaATGGCTGCGCCATGCGGACGATCGTGTCATAGAACGCCAAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTAAGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCAAGGCCAACAATGCCCGCCATCGCATAATCCAGATAGGAGATCTACAGCTCTTCCTc                                    >  1:203600/1‑251 (MQ=255)                                                                                                                                                                                                                     aTGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCAAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCCACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTACTCAATg                                >  2:243948/1‑251 (MQ=255)                                                                                                                                                                                                                        gcttcgcCATGCGGACGATCGTGTCATAGACCGCCAATTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGTCACGGTCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTGCATTGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACAGCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGTAGCTCTTCAGCTCTTCCTCAATGTTg                             <  1:305951/247‑1 (MQ=255)                                                                                                                                                                                                                                 tGCGGACGATCGTGTCATAGACCGCCAAGTCACCATGGTGATGGTAGTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCACAATGTTGACCGgtg                      <  2:327033/249‑1 (MQ=255)                                                                                                                                                                                                                                    ggACGATCGTGTCATATACCGCCAAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCGCTTCAGCTCTTCCTCAATGTTGACCGGTGTAAtt                 <  1:122926/251‑1 (MQ=255)                                                                                                                                                                                                                                    ggACGATCGTGTCATAGACCGCCAAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTAGGACATCTGGCAGCGCACGGCCAACAATGACCGACGTCGCATAATCCAGATCGGGGCTATTCAGCTCTTCCTCAATGTTTGCCGGTGAAAtt                 >  2:75408/1‑251 (MQ=255)                                                                                                                                                                                                                                        gATCGAGTCATAGACCGCCAAGTCACCCTGGGGCTGGAATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGATTCAGGCCCTCTCGGACATCTGGCAGCGCCCGGCCAACAATGACCGACATCGCCTAATCCAGATAGGAGCACTTCAGCCCTTCCTCCCTGTTGTCCgggtgaatttctt             >  2:23184/1‑240 (MQ=255)                                                                                                                                                                                                                                           cGTGTCATAGACCGCCAAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCGGTGTAATTtctcgc           <  2:235953/250‑3 (MQ=255)                                                                                                                                                                                                                                                   aGACCGCCAAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCGGTGTAATTTCTCTCGCAAGGTCg  >  2:374293/1‑251 (MQ=255)                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                   CAGGTTAGGAATTTTGGTTGGCATGACGTCCGGAATTTTTTCCGTGCCGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCGGTGTAATTTCTCTCGCAAGGTCG  >  NC_000913/2338923‑2339415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑