Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,829,887	G→A	A293A (GCC→GCT)	astA ←	arginine N‑succinyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,829,887	0	G	A	97.6%	114.6 / NA	42	A293A (GCC→GCT)	astA	arginine N‑succinyltransferase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (18/23);  total (19/23)
Fisher's exact test for biased strand distribution p-value = 4.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01

CATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATT‑GGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGAT‑GTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAG  >  NC_000913/1829649‑1830130                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                     cATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGGTGCCGGGTCGGGACGCACCAGACCCACGCGGAAATGGGGGAAATTTTCATT‑AGCGACcaggc                                                                                                                                                                                                                                            >  1:120509/1‑249 (MQ=255)  aTAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATT‑AGCGACcaggcagg                                                                                                                                                                                                                                         <  1:92765/251‑1 (MQ=255)    aaCACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATT‑AGCGACCAGGCAGGCt                                                                                                                                                                                                                                       <  1:305924/251‑1 (MQ=255)     acacCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATT‑AGCGACCAGGCAGGCTg                                                                                                                                                                                                                                      >  1:171072/1‑251 (MQ=255)          tCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATT‑AGCGACCAGGCAGGGTgggga                                                                                                                                                                                                                                  >  1:57398/1‑248 (MQ=255)            gCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCGCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATT‑AGCGACCAGGCAGGCTGGGAAATc                                                                                                                                                                                                                               <  2:332196/251‑1 (MQ=255)             cccGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATT‑AGCGACCAGGCAGGCTGGGAAATCg                                                                                                                                                                                                                              <  1:242736/251‑1 (MQ=255)                gATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACCCTCGGGTGCCGGATCGGTACGCACCAGCACCACGCGGGAATGGGGATAATTTTCATT‑AGCGACCAGGCAGGCTGGGAAATCGccc                                                                                                                                                                                                                           >  1:90984/1‑251 (MQ=255)                 aTACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTTCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGAGGTTAAAATCAAACGCTCGGTTGCCGGATCGGGACGCACCAACACCACACGGAAATGGGGATAATTTTAATT‑AACAACCAGCCAGTCTGGGTAATCGcccc                                                                                                                                                                                                                          >  1:4923/1‑250 (MQ=255)                         ttACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCCCGGTTGCCGGATCGGTACGCCCCAGCACCACGCGGGAATAGTGATAATTTTCATT‑AGCGACCAGGCAAGCTTGGAAAtaacccttcgcaagc                                                                                                                                                                                                                  >  2:281826/1‑237 (MQ=255)                           aCGCTTCCCACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCCCAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATT‑AGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTg                                                                                                                                                                                                                <  1:210437/251‑1 (MQ=255)                                       cgATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGAGCACCAGACGAACGCGATCCACGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGATAAAATCCAACGCTCGGTTGCCGGATCGGTACGCACAAGAACCACGCGGAAATGGGGATAATTTTTATT‑ATCGACCAGGCAGGCTGGGGAAACGCCCTGCGCAGGGTGCCCCTCTGCCAc                                                                                                                                                                                                    >  1:167605/1‑251 (MQ=255)                                                  ggctcGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCTCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATT‑AGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGcc                                                                                                                                                                                         <  1:178587/247‑1 (MQ=255)                                                                 cACCGTTAATCCCTAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGGTGACGGATCGGTGCGCACCAGCACCCCGCGGAAAAGGTGGTAATTTTCATT‑AGGGACCAGGCAGGCTGGGAAAACGCCCCGCGCAAGCTTGCCTTCTGCCACTTCCACCCGCCCGCTTTTACGGGTgg                                                                                                                                                                          >  2:243905/1‑251 (MQ=255)                                                                                      tGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTTAAATCAAACGCTCGGTTGCCGGGACGGGACGCCCCAGCACCCCGCGGAAAAGGTGAAAATTTTTATTAGGC‑ACCAGGCAGGCTGGGAAAACGCCCTCCGCCGGGTGGCCTTTTGCCCCTTCCACCCACCGGCTTTTACggggggggaggaaccggtcgacgttac                                                                                                                                                     >  2:104523/1‑226 (MQ=255)                                                                                                 cgtctGCGCACAGGCGCGCCGGCCGATCGCGATCCCCGGCGTGGCATGGGAGGGCATCCAGTTTTGCGGCGGTTAAAATTAAGCGCGCGGTTGCCGTATCGGTACGCACCAGCCCCACGCGGAAATGGTGATAATTTTCATT‑AGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCGGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTc                                                                                                                                          <  2:298355/249‑1 (MQ=255)                                                                                                 cctcTGCGCACAGGCGCACCAGACGAACGCGATCCCCGTCGTGTCAGTGGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGAGCGGTACGCCCCAGCACCACGCGGAAATGGTGATAATTTTCATT‑AGCGACCAGGCAGGCTGGGAAATCGCCCTGCGGAGGCTGCCGTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGc                                                                                                                                             <  2:194658/248‑1 (MQ=255)                                                                                                 cctcTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAACCGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTGCATT‑AGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTGCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGc                                                                                                                                             <  2:362895/248‑1 (MQ=255)                                                                                                             ggCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATT‑AGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCa                                                                                                                              <  1:104523/251‑1 (MQ=255)                                                                                                             ggCGCACCAGACGAACGCATTCCCCGGCGTGGCATTTGAGGTCTTCCGTTTGTGCGGCGGTGAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATT‑AGCGCCCAGGCAGGCTGGGAAATCGCCCTGCGCGGGCTGCCCTTCTGCCACTTCCTCCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCTGCCCACCGTCa                                                                                                                              <  2:347135/251‑1 (MQ=255)                                                                                                                                            gCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGGTCGGTACGCACCAGCACCACGCGGAAATGGGGATAATTTTCATT‑AGCGACCAAGCAGGCTGGGAAAACGCCCTGCGCAGGCTGCCCTTCTGCCACCTCCACCAGCCGACTTTTACGGGTGGCGCGCACGCGGTCGCTTGCACACCCAAGCGTCGGCCCCCCCTCAAA‑ATAGTCGATAAAATTACGGTAGCGgaaa                                                                                               >  2:262738/1‑250 (MQ=255)                                                                                                                                                ttgggggCATCCCGTTGGGCGGCGTATAAAATCACACTCTCGGTGGCGGGAGCGGTACGCACCAGCACCACTCGGAAAGGGGGATAATTTGCATG‑AGGTACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCGGCCCTTCTGCCGCTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGGCGATGTCACACTCAAGCGTCGGTCCACCGTCAAAGAT‑GTCGATAGAGTTACGGTAGCGAAAACctt                                                                                           <  2:190904/246‑1 (MQ=255)                                                                                                                                                    gggCATCCCGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATT‑AGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGAT‑GTCGATATAGTTACAGTAGCGAAAACCttctttc                                                                                      <  2:120509/251‑1 (MQ=255)                                                                                                                                                    gggCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATT‑AGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGAT‑GTCGATATAGTTACGGTAGCGAAACCCttctttc                                                                                      <  1:56476/251‑1 (MQ=255)                                                                                                                                                        aTCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATT‑AGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGAT‑GTCGAtata                                                                                                               <  2:18202/222‑1 (MQ=255)                                                                                                                                                          ccAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATT‑AGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGGCGGCCCCCCGTCAAAGAT‑GTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTcccgc                                                                                >  1:271330/1‑248 (MQ=255)                                                                                                                                                                    ggcggTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATT‑AGCGACCAGGCATGCTGGGAAATCGCCCGGCGCAGGCTGCCCTTCTTCCACTTCCACCAGCCGACTTTTACGGAGGGCGCGCACGCGGTCGATGTCACTCTCAAGCGTCGGCCCTCCGCCAAAGAT‑GTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCTCCGCGCggg                                                                      <  1:222350/251‑1 (MQ=255)                                                                                                                                                                      cggTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATT‑AGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGAT‑GTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGc                                                                     >  1:260173/1‑250 (MQ=255)                                                                                                                                                                       ggTTAAAATCAAACTCGCGGTTGCCGGATCGGTACGCTCCAGCCCCACGCGGAAATGGGGATAATTTTCATT‑AGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGAT‑GTCGATATAGTTACGGGAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAg                                                                   <  2:79933/251‑1 (MQ=255)                                                                                                                                                                              aTCAAACGCTCGGTTGCCGGATCGGTCCGCACCAGCACCACGCGGAAATGGAGATAATTTTCATT‑AGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGAT‑GTCGATATAGTTACGGGAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGt                                                            >  1:109495/1‑251 (MQ=255)                                                                                                                                                                              aTCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATT‑AGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGAT‑GTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGt                                                            >  1:74165/1‑251 (MQ=255)                                                                                                                                                                                    cGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGGGATAAATTTCATT‑AGCGACCAGGCAGGATGGGTAATCGCACTGCGCAGGCTGCCCTTCTGCCCCTTCCACCAGCCGACTTTTACGGATCGCGCGCACGCGGTCGATGTCGCACTCAAGCGTCGGCCCACCGTCCAAGAT‑GGCGATATAGTTACGGTGACGAACACCTTCTTTCCACAGCACAGCACGGGCAGGCGCgggtttcgg                                                      >  1:37011/1‑244 (MQ=255)                                                                                                                                                                                            tGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATT‑AGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGGCGATGTCACCCTAAAGCGTCGGCCCACCGTCAAAGAT‑GTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCCCGGGCAGGCGAGGGTTGCGGATGAACCt                                              >  2:263089/1‑251 (MQ=255)                                                                                                                                                                                                ggATCGGTACGCACCAGCACCACGCGGAAAGGGTGATAATTTTCCTT‑AGCGACCAGGCAGGCTGGGAAATCGCCCTGTGCAGCCTGCCCGTCTGCCACTCCCACCAGCCGACTTTTACGGATGGCGTGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCCCCGTCAAAGAT‑GTCGATATAGTTACGGTAGCGAAAACATTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGACGTACCTGAcc                                          <  2:215292/251‑1 (MQ=255)                                                                                                                                                                                                     ggTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATT‑AGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGAT‑GTCGCTATAGTTACGGTAGCGAAAACCTTCATTCTCCAGCACCGCCCGGGCAGGCGAGGTTTGCGGaagttactgacccacgg                                     >  2:241620/1‑235 (MQ=255)                                                                                                                                                                                                              cagcaccaCGCGGAAAGGGTGATAATTTTCATT‑AGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGAT‑GTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTggg                            <  2:57398/251‑1 (MQ=255)                                                                                                                                                                                                                  accacGCGGAAATGGTGATAATTTTCATT‑AGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGAT‑GTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTc                        <  1:297076/251‑1 (MQ=255)                                                                                                                                                                                                                     ccgcgGAAATGGTGATAATTTCCATT‑AGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGAT‑GTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTg                     <  2:98964/250‑1 (MQ=255)                                                                                                                                                                                                                      cgcgGAAATGGTGATAATTTTCATT‑AGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGAT‑GTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTgg                    >  2:135381/1‑251 (MQ=255)                                                                                                                                                                                                                                 tGATAATTTTCATT‑AGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGAT‑GTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTg         >  1:236261/1‑251 (MQ=255)                                                                                                                                                                                                                                     aaTTTTCATT‑AGCGACCGGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCCCTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGAT‑GTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGta     <  2:90984/251‑1 (MQ=255)                                                                                                                                                                                                                                         ttCATT‑AGCGACCATGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTGACGGATGGCGCGCACGCGGTCGATGTCCCACTCAAGCGTCGGCCAACCGTCAAAGAT‑GTCGATATAGTTACGGTAGCGAAAACCTGCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAg  <  1:184362/250‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                     CATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATT‑GGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGAT‑GTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAG  >  NC_000913/1829649‑1830130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑