Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,358,859 T→C Y110C (TAT→TGT)  puuP ← putrescine:H(+) symporter PuuP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,358,8590TC90.9% 84.6 / ‑5.9 33Y110C (TAT→TGT) puuPputrescine:H(+) symporter PuuP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (15/15);  minor base A (2/1);  total (17/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.08e-01

GTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACC  >  NC_000913/1358619‑1359097
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                               
gTGCCAACGCCTTCTCCTTTAGGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATGGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCcagcag                                                                                                                                                                                                                                       <  2:189623/251‑1 (MQ=255)
        gCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACACACAGGGAATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGCTAAAATGACGGCGCAGGGTACCACCCCCACCCACAGCAGCACATCCGCGAAGAGGGGGGGGAGAAAGGATTTCGCCCACCAAACGGTTATTATCGGGAAAAAGAGACAATCCAGCAGCGATTAcc                                                                                                                                                                                                                               >  1:19751/1‑251 (MQ=255)
         ccTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTCTTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGCTAAAATGGCGGCGCAGGTTACCCCCCAACCCCACGCCGGCACTTCCCGGAAGAGGGGGGAGAGATAAATTTTCGCCAAACAGACGATAATAATCGGGCAAAAAAGACAATCCAGCAGCGATGAcc                                                                                                                                                                                                                               >  2:32282/1‑250 (MQ=255)
                           cccTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTAGTGAAGTTTGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGATAGATATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCACCCGACCAGAAATCCGAc                                                                                                                                                                                                            <  2:216938/251‑1 (MQ=255)
                                               aaaCACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAAGCCGACGTGCGGGTTAATCGACtttt                                                                                                                                                                                        <  1:302076/251‑1 (MQ=255)
                                                aaCACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTAACACCCACAACCACGGCGGCACTTCCGGGAAGAGGGCGGGGAGATAGGTTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGGGAAAATCCAGCAGCGATGACCAGCCGACCATAAAACCGACGAGCGGGGTAATCGACTTTTg                                                                                                                                                                                       >  1:346198/1‑251 (MQ=255)
                                                aaCACCACCATGATGGAGATTGGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTg                                                                                                                                                                                       <  1:109720/251‑1 (MQ=255)
                                                                     tGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATAGGGACGAAGGTTACCACCCCCACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGGGGTAGATTTTTGCCACAAAGGCGTTTATCATCGAGAAAAAAGAACAAACCCCCAGCGATGACCAGACCACCAAAAATACGCCGTGAGGGTTAATAGACATTTGcgcggtggtaatagccgcac                                                                                                                                                                   >  2:259975/1‑234 (MQ=255)
                                                                                  aCAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGGGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGGGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCCCCGGAAAc                                                                                                                                                     >  1:192251/1‑251 (MQ=255)
                                                                                   cAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGGCGAAGGGTTCCACCCACACCCCCCGCGGCCATTCCGGGAAGAGGGGGGGGAGATCGATTTTTGCCCACCAAACGTTAATCATCGGCAAAAAGAGACAAACCCACAGCGGTGCCCAGCCCCCCATAAAAACGACCGGCGGGGTAATCGGCCTTTGCGCGTAGGGATAGGCCGCAcccgaccccgggacac                                                                                                                                                    >  2:17581/1‑237 (MQ=255)
                                                                                    aGGGTATTGAAGTTAGCGACCAGGTTGCAGGTCTTCAGATGCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTg                                                                                                                                                   <  2:273143/251‑1 (MQ=255)
                                                                                                       ccAGGTTTACTCTCGTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAg                                                                                                                                <  1:369903/251‑1 (MQ=255)
                                                                                                       ccAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAg                                                                                                                                <  1:158007/251‑1 (MQ=255)
                                                                                                              gACGCCCTGCAGATTCGCGGCTGTTAAAATGGCGACGAAGGTTACCTCCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATa                                                                                                                          <  2:61995/250‑1 (MQ=255)
                                                                                                                                   ggTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCcgcc                                                                                                    >  1:96626/1‑250 (MQ=255)
                                                                                                                                       aaaaTGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGCACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCcgcacgcac                                                                                               >  2:259259/1‑245 (MQ=255)
                                                                                                                                                                     cacGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTc                                                                 >  1:54829/1‑251 (MQ=255)
                                                                                                                                                                      acGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGta                                                                                                                                                                             >  2:108549/1‑143 (MQ=255)
                                                                                                                                                                      acGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGta                                                                                                                                                                             <  1:108549/143‑1 (MQ=255)
                                                                                                                                                                      acGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCg                                                                >  1:305915/1‑251 (MQ=255)
                                                                                                                                                                            gcACTTCGGGGAAGAGGCCGGAGAGATAGATTTCCGCAAACAAGACGTTAACCATCGGCAAAAAGAGAAAATCCAGCAGCGATGCCCAGCCGACCATAAACCCGACGTGCGGGTTAATCGTCTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACGGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATg                                                          <  2:192251/251‑1 (MQ=255)
                                                                                                                                                                             cACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGCCCAGCCGACCATAAACCCGACGTGGGGGTTAATCGCCTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCATTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGc                                                         <  1:173208/251‑1 (MQ=255)
                                                                                                                                                                                  ccGGGGAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCgg                                                      >  2:367940/1‑249 (MQ=255)
                                                                                                                                                                                                    gatagatTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATc                                   >  1:12757/1‑250 (MQ=255)
                                                                                                                                                                                                     atagatTTCCGCCAAAAAGGCGTTAACCATCGGAAAAAAGAGACAACCCAGCAGCGAGGACCAGCCAACCATAAATCCGCCGTGCGGGTTAATCGACTTTTGCGCGTGGGTATAGGCCGAACCGGCCTCCGGAAACTGGCAAACCAGTTTGCCGAAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTTACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCaa                                 <  1:106092/251‑1 (MQ=255)
                                                                                                                                                                                                       agatTTTCGCCAACAAGACGTTAATCATCGGCAAAACGAGAAAATCCAGCAGCGATGACCAGCCGTCCATAAATCCGACGAGCGGGATAATCTACTTTTGCGCGTAGGTATAGGACGAACCGGCCTCCGGAAACAGGCGAACACGTTTGCCGTAGCTGATAGCGGGAAACAGCACACCAGCCACCGCGAGCAAATAGGACGCCGAAACGTGACCGTCGCCAATGCCGGAGACAATACCaaaaataacaca                                >  2:101176/1‑242 (MQ=255)
                                                                                                                                                                                                             tccccaacacaaataaaaTCATCGGCAAAAAGAGACAATCCAGCAGCGAAGCCCAGCCGACCAAAAACCCGCCGGGCGGGTAAATCGACTTTTGCGCGTGGGTATAGGCCGACCCGGCCGCCGGAAACTGGCGACCCAGTTTGCCGTAGCGGATAGCGGTAAACAGACCACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACg                            <  2:346198/232‑1 (MQ=255)
                                                                                                                                                                                                                       gACGTTAATCATCGCCAAAAAGAGACAACCCAGCAGCGATGACCAGCCTACCATAAACCCGACGTGCGGTTTAATCTACTTTCGCGCGTAGGTAGAGGCCTAACCGGCCCCCGTAACCTGGGGACCCAGTGTGCCGTAGCTGATAGCGGGAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGGGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTga               <  1:26526/251‑1 (MQ=255)
                                                                                                                                                                                                                          gcgtaaaacagcggaaaaaaGAGACAATCCAGCGGCGATGACCCTCGGACAATAAACCGGAGGGGCGGGTTAATCGCCTTTGGCGCGTAGGTATAGGCGTAACCGGCCTCGGGAACCTGGCGACCCATTTGGCCTTAGTCGAAAGCGGAAAACAGCAACGCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGGCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTgag              <  1:40367/236‑1 (MQ=255)
                                                                                                                                                                                                                            tAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCCGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAACGAATCAAATACGGTCATCGGCGTGAGAt            >  2:206327/1‑249 (MQ=255)
                                                                                                                                                                                                                              atcatcGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGc        >  1:280253/1‑251 (MQ=255)
                                                                                                                                                                                                                                   cGGCAAAAAGAGACAATCCGGCAGGGTTGCCCAGCCGACCATAAATCCGTCGTGCGGGTTAATCGACTTTGGCGCGTAGGTAAAGTCCGAACCGGCCCCCGAAAACTGGCGAACCAGTTGGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGa    <  1:224857/250‑1 (MQ=255)
                                                                                                                                                                                                                                    ggCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAgccc  >  1:363145/1‑248 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                               
GTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACC  >  NC_000913/1358619‑1359097

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: